Frontiers in Pharmacology,
Journal Year:
2025,
Volume and Issue:
16
Published: April 3, 2025
Background
Non-small
cell
lung
cancer
(NSCLC)
is
one
of
the
most
prevalent
and
deadly
malignancies
worldwide.
In
previous
studies,
indacaterol,
a
drug
used
to
manage
chronic
obstructive
pulmonary
disease,
has
shown
antitumor
activity.
However,
its
role
in
context
NSCLC
remains
underexplored.
This
study
aimed
investigate
indacaterol’s
mechanisms
potential
therapeutic
effects
treatment.
Methods
Expression
profiles
clinical
information
from
TCGA
database
were
analyzed
explore
impact
SLC2A1
gene
on
progression
NSCLC.
The
expression
levels
GLUT1
protein,
encoded
by
gene,
MCT4
SLC16A3
both
normal
tissues.
Techniques
such
as
cellular
thermal
shift
assay
(CETSA),
immunofluorescence,
Western
blotting
employed
assess
interaction
between
indacaterol
GLUT1.
Immunohistochemistry
was
human
lines
observed
through
wound
healing
colony
formation
assays.
Additionally,
animal
experiments
combined
with
PD-L1
inhibitors
conducted
evaluate
vitro
vivo
.
Results
Analysis
data
revealed
that
promoting
may
work
concert
MCT4.
Indacaterol
significantly
inhibited
viability
cells
concentration-dependent
manner.
Molecular
modeling
CETSA
further
indicated
bind
affect
expression.
suggested
also
reduces
MCT4,
suggesting
diminish
capacity
tumors
reprogram
stromal
metabolism.
confirmed
combination
synergistically
proliferation
invasion
cells.
Conclusion
Indacaterol,
inhibitor
GLUT1,
significant
Moreover,
immune
checkpoint
enhanced
inhibitory
Our
provides
scientific
evidence
supporting
application
novel
strategy
for
Clinical Epigenetics,
Journal Year:
2024,
Volume and Issue:
16(1)
Published: May 29, 2024
Abstract
Lactic
acid,
traditionally
considered
as
a
metabolic
waste
product
arising
from
glycolysis,
has
undergone
resurgence
in
scientific
interest
since
the
discovery
of
Warburg
effect
tumor
cells.
Numerous
studies
have
proved
that
lactic
acid
could
promote
angiogenesis
and
impair
function
immune
cells
within
microenvironments.
Nevertheless,
precise
molecular
mechanisms
governing
these
biological
functions
remain
inadequately
understood.
Recently,
been
found
to
induce
posttranslational
modification,
lactylation,
may
offer
insight
into
acid's
non-metabolic
functions.
Notably,
modification
proteins
by
lactylation
emerged
crucial
mechanism
which
lactate
regulates
cellular
processes.
This
article
provides
an
overview
acidification,
outlines
potential
“writers”
“erasers”
responsible
for
protein
presents
patterns
across
different
organisms,
discusses
diverse
physiological
roles
lactylation.
Besides,
highlights
latest
research
progress
concerning
regulatory
pathological
processes
underscores
its
significance
future
investigations.
Clinical and Translational Medicine,
Journal Year:
2024,
Volume and Issue:
14(3)
Published: March 1, 2024
Abstract
Heightened
lactate
production
in
cancer
cells
has
been
linked
to
various
cellular
mechanisms
such
as
angiogenesis,
hypoxia,
macrophage
polarisation
and
T‐cell
dysfunction.
The
lactate‐induced
lactylation
of
histone
lysine
residues
is
noteworthy,
it
functions
an
epigenetic
modification
that
directly
augments
gene
transcription
from
chromatin.
This
originating
effectively
fosters
a
reliance
on
transcription,
thereby
expediting
tumour
progression
development.
Herein,
this
review
explores
the
correlation
between
characteristics,
revealing
innovative
process
enhances
vulnerability
malignancy.
Moreover,
imperative
acknowledge
paramount
importance
acknowledging
therapeutic
methodologies
for
proficiently
managing
by
precisely
targeting
signalling.
comprehensive
illuminates
crucial
yet
inadequately
investigated
aspect
lactylation,
providing
valuable
insights
into
its
clinical
ramifications
prospective
interventions
centred
lactylation.
Signal Transduction and Targeted Therapy,
Journal Year:
2025,
Volume and Issue:
10(1)
Published: Feb. 11, 2025
Abstract
Accumulated
evidence
has
implicated
the
diverse
and
substantial
influence
of
lactate
on
cellular
differentiation
fate
regulation
in
physiological
pathological
settings,
particularly
intricate
conditions
such
as
cancer.
Specifically,
been
demonstrated
to
be
pivotal
molding
tumor
microenvironment
(TME)
through
its
effects
different
cell
populations.
Within
cells,
impacts
signaling
pathways,
augments
shuttle
process,
boosts
resistance
oxidative
stress,
contributes
lactylation.
In
various
populations,
interplay
between
immune
cells
governs
processes
differentiation,
response,
surveillance,
treatment
effectiveness.
Furthermore,
communication
stromal/endothelial
supports
basal
membrane
(BM)
remodeling,
epithelial-mesenchymal
transitions
(EMT),
metabolic
reprogramming,
angiogenesis,
drug
resistance.
Focusing
production
transport,
specifically
dehydrogenase
(LDH)
monocarboxylate
transporters
(MCT),
shown
promise
Inhibitors
targeting
LDH
MCT
act
both
suppressors
enhancers
immunotherapy,
leading
a
synergistic
therapeutic
effect
when
combined
with
immunotherapy.
The
review
underscores
importance
progression
provides
valuable
perspectives
potential
approaches
that
target
vulnerability
metabolism,
highlighting
Heel
Achilles
for
cancer
treatment.
Advanced Science,
Journal Year:
2024,
Volume and Issue:
11(14)
Published: Jan. 28, 2024
Abstract
Intracellular
delivery
of
nano‐drug‐carriers
(NDC)
to
specific
cells,
diseased
regions,
or
solid
tumors
has
entered
the
era
precision
medicine
that
requires
systematic
knowledge
nano‐biological
interactions
from
multidisciplinary
perspectives.
To
this
end,
review
first
provides
an
overview
membrane‐disruption
methods
such
as
electroporation,
sonoporation,
photoporation,
microfluidic
delivery,
and
microinjection
with
merits
high‐throughput
enhanced
efficiency
for
in
vitro
NDC
delivery.
The
impact
characteristics
including
particle
size,
shape,
charge,
hydrophobicity,
elasticity
on
cellular
uptake
are
elaborated
several
types
systems
aiming
hierarchical
targeting
vivo
reviewed.
Emerging
ex
human/animal‐derived
pathophysiological
models
further
explored
highly
recommended
use
studies
since
they
might
mimic
features
fill
translational
gaps
animals
humans.
exploration
modern
microscopy
techniques
precise
nanoparticle
(NP)
tracking
at
cellular,
organ,
organismal
levels
informs
tailored
development
NDCs
application
clinical
translation.
Overall,
integrates
latest
insights
into
smart
nanosystem
engineering,
physiological
models,
imaging‐based
validation
tools,
all
directed
towards
enhancing
efficient
intracellular
NDCs.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
2(1)
Published: Jan. 1, 2024
Abstract
Immunotherapy,
specifically
immune
checkpoint
inhibitors,
is
revolutionizing
cancer
treatment,
achieving
durable
control
of
previously
incurable
or
advanced
tumors.
However,
only
a
certain
group
patients
exhibit
effective
responses
to
immunotherapy.
Anti‐angiogenic
therapy
aims
block
blood
vessel
growth
in
tumors
by
depriving
them
essential
nutrients
and
effectively
impeding
their
growth.
Emerging
evidence
shows
that
tumor
vessels
structural
functional
abnormalities,
resulting
an
immunosuppressive
microenvironment
poor
response
Both
preclinical
clinical
studies
have
used
anti‐angiogenic
agents
enhance
the
effectiveness
immunotherapy
against
cancer.
In
this
review,
we
concentrate
on
synergistic
effect
therapies
management,
dissect
direct
effects
underlying
mechanisms
recruiting
activating
cells,
discuss
potential
improve
Lastly,
outline
challenges
opportunities
for
strategy
Considering
increasing
approval
combination
treating
cancers,
comprehensive
review
would
be
timely
important.
