Two‐Photon Laser Printing to Mechanically Stimulate Multicellular Systems in 3D DOI Creative Commons
Federico Colombo, Mohammadreza Taale, Fereydoon Taheri

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(19)

Published: March 4, 2024

Abstract Biological activities take place in 3D environments, where cells interact various directions a defined, often microstructured, space. A sub‐millimeter‐sized stretching device is developed to mechanically stimulate structurally restricted, soft multicellular microenvironment investigate the effect of defined cyclic mechanical forces on system. It consists multi‐material microstructure made Polydimethylsiloxane (PDMS) and gelatine‐based hydrogel, which printed using 2‐photon polymerization (2PP) method. The structures are first characterized microscopically study different printing parameters. Using 2PP, organotypic cell cultures then directly into hydrogel create true culture systems. These systems stimulated with cantilever by indenting at positions. change morphology actin orientation when exposed stretch, even within short timescales 30 min. As proof concept, Medaka retinal organoid encapsulated same structure demonstrate that preformed organoids can be this results highlight capability 2PP for manufacturing multifunctional devices control micrometer resolution thus mimic stresses as they occur vivo.

Language: Английский

Organization, dynamics and mechanoregulation of integrin-mediated cell–ECM adhesions DOI
Pakorn Kanchanawong, David Calderwood

Nature Reviews Molecular Cell Biology, Journal Year: 2022, Volume and Issue: 24(2), P. 142 - 161

Published: Sept. 27, 2022

Language: Английский

Citations

261
Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions DOI Creative Commons

Peilin Lu,

Dongxue Ruan,

Meiqi Huang

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: July 1, 2024

The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements potential therapeutic applications, focusing primarily on two areas: emerging cell-based therapies promising non-cell modalities. Within context cell therapy, discuss capacity overcome existing translational challenges faced by mainstream therapy paradigms, provide a detailed discussion advantages principal design considerations for boosting efficacy as well list specific examples different disease scenarios. We then explore drug delivery, physical intervention therapies, other areas (e.g., bioadhesives, artificial tissues, biosensors), emphasizing utility beyond mere delivery vehicles. Additionally, complement our latest progress clinical application outline future research directions, particularly terms integration with advanced biomanufacturing This review aims present comprehensive view critical insights into selection both tailored meet requirements diverse diseases situations.

Language: Английский

Citations

83
Stimuli-Responsive Hydrogels: The Dynamic Smart Biomaterials of Tomorrow DOI Creative Commons
Myriam Neumann,

Greta Di Marco,

Dmitrii Iudin

et al.

Macromolecules, Journal Year: 2023, Volume and Issue: 56(21), P. 8377 - 8392

Published: Oct. 18, 2023

In the past decade, stimuli-responsive hydrogels are increasingly studied as biomaterials for tissue engineering and regenerative medicine purposes. Smart can not only replicate physicochemical properties of extracellular matrix but also mimic dynamic processes that crucial regulation cell behavior. Dynamic changes be influenced by hydrogel itself (isotropic vs anisotropic) or guided applying localized triggers. The resulting swelling–shrinking, shape-morphing, well patterns have been shown to influence function in a spatiotemporally controlled manner. Furthermore, use bioinks 4D bioprinting is very promising they allow biofabrication complex microstructures. This perspective discusses recent cutting-edge advances current challenges field smart engineering. Additionally, emerging trends potential future directions addressed.

Language: Английский

Citations

53
Extracellular Matrix Cues Regulate Mechanosensing and Mechanotransduction of Cancer Cells DOI Creative Commons
Claudia Tanja Mierke

Cells, Journal Year: 2024, Volume and Issue: 13(1), P. 96 - 96

Published: Jan. 2, 2024

Extracellular biophysical properties have particular implications for a wide spectrum of cellular behaviors and functions, including growth, motility, differentiation, apoptosis, gene expression, cell–matrix cell–cell adhesion, signal transduction mechanotransduction. Cells not only react to unambiguously mechanical cues from the extracellular matrix (ECM), but can occasionally manipulate features in parallel with biological characteristics, thus interfering downstream matrix-based both physiological pathological processes. Bidirectional interactions between cells (bio)materials vitro alter cell phenotype mechanotransduction, as well ECM structure, intentionally or unintentionally. Interactions mechanics vivo are importance variety diseases, primarily cancer. Stiffness values normal cancerous tissue range 500 Pa (soft) 48 kPa (stiff), respectively. Even shear flow increase 0.1–1 dyn/cm2 (normal tissue) 1–10 (cancerous tissue). There currently many new areas activity tumor research on various length scales, which highlighted this review. Moreover, complexity cancer is reduced common different tumors characteristics identify main pathways interaction. This all contributes standardization mechanotransduction models approaches, which, ultimately, increases understanding complex Finally, effects mechanics–biology pairing key insights clinical practice treatment and, consequently, translation.

Language: Английский

Citations

33
The role and regulation of integrins in cell migration and invasion DOI
Megan R. Chastney, Jasmin Kaivola, Veli‐Matti Leppänen

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Language: Английский

Citations

22
Extracellular matrix dynamics: A key regulator of cell migration across length-scales and systems DOI
Dharma Pally, Alexandra Naba

Current Opinion in Cell Biology, Journal Year: 2024, Volume and Issue: 86, P. 102309 - 102309

Published: Jan. 5, 2024

Language: Английский

Citations

19
Harnessing developmental dynamics of spinal cord extracellular matrix improves regenerative potential of spinal cord organoids DOI
Zheng Sun,

Zhenni Chen,

Man Yin

et al.

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(5), P. 772 - 787.e11

Published: April 1, 2024

Language: Английский

Citations

19
Interpenetrating networks of fibrillar and amorphous collagen promote cell spreading and hydrogel stability DOI
Lucia G. Brunel,

Chris M. Long,

Fotis Christakopoulos

et al.

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Recent Progress of the Vat Photopolymerization Technique in Tissue Engineering: A Brief Review of Mechanisms, Methods, Materials, and Applications DOI Open Access
Ying Li, Xueqin Zhang, Xin Zhang

et al.

Polymers, Journal Year: 2023, Volume and Issue: 15(19), P. 3940 - 3940

Published: Sept. 29, 2023

Vat photopolymerization (VP), including stereolithography (SLA), digital light processing (DLP), and volumetric printing, employs UV or visible to solidify cell-laden photoactive bioresin contained within a vat in point-by-point, layer-by-layer, manner. VP-based bioprinting has garnered substantial attention both academia industry due its unprecedented control over printing resolution accuracy, as well rapid speed. It holds tremendous potential for the fabrication of tissue- organ-like structures field regenerative medicine. This review summarizes recent progress VP fields tissue engineering First, it introduces mechanism photopolymerization, followed by an explanation technique commonly used biomaterials. Furthermore, application was discussed. Finally, challenges facing are discussed, future trends projected.

Language: Английский

Citations

26
Synergistic Influence of Fibrous Pattern Orientation and Modulus on Cellular Mechanoresponse DOI
Qian Sun,

Xiaokai Pan,

Peng Wang

et al.

Nano Letters, Journal Year: 2024, Volume and Issue: 24(21), P. 6376 - 6385

Published: May 14, 2024

The fibrous extracellular matrix (ECM) is vital for tissue regeneration and impacts implanted device treatments. Previous research on biomaterials shows varying cellular reactions to surface orientation, often due unclear interactions between topography substrate elasticity. Our study addresses this gap by achieving the rapid creation of hydrogels with diverse topographies moduli through a printing strategy. Cells exhibit heightened traction force nanopatterned soft hydrogels, particularly randomly distributed patterns compared regular hydrogels. Meanwhile, stiff featuring an aligned topography, optimal mechanosensing observed random topography. Mechanistic investigations highlight that force-sensing adhesion are influenced interplay pattern deformability focal subsequently mediating stem cell differentiation. findings importance combining modulus guide behavior in designing advanced engineering biomaterials.

Language: Английский

Citations

13