Development of label-free cell tracking for discrimination of the heterogeneous mesenchymal migration DOI Creative Commons

Sota Endo,

S. Yamamoto,

Hiromi Miyoshi

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0320287 - e0320287

Published: March 31, 2025

Image-based cell phenotyping is fundamental in both biology and medicine. As cells are dynamic systems, based on static data complemented by extracted from time-dependent characteristics. We developed a label-free automatic tracking method for phase contrast images. examined the possibility of using motility-based discrimination to identify different types mesenchymal migration invasive malignant cancer non-cancer cells. These were cultured plastic tissue culture vessels, motility parameters trajectories with tracking. Correlation analysis these identified characteristic HT1080 fibrosarcoma 3T3-Swiss fibroblast lines. The parameter “sum turn angles,” combined “frequency turns” at shallow angles “migration speed,” proved effective highlighting characteristics It revealed differences their mechanisms generating propulsive forces. requirements characterize included spatiotemporal resolution segmentation tracking, capable detecting polarity changes associated morphological alterations body displacement. With proposed here, curve computed quadratic angles” turns below 30°” gave best performance 94% sensitivity. Cell process related not only but also healing growth. methodology easy use, enabling anyone without professional skills image analysis, large training datasets, or special devices. has potential application broad range applications basic research. Validating expandability this migration, including scheme force generation, an important consideration future study.

Language: Английский

Cell polarity changes in cancer initiation and progression DOI Creative Commons
Florent Péglion, Sandrine Etienne‐Manneville

The Journal of Cell Biology, Journal Year: 2023, Volume and Issue: 223(1)

Published: Dec. 13, 2023

Cell polarity, which consists of the morphological, structural, and functional organization cells along a defined axis, is feature healthy tissues. In contrast, abnormal polarity hallmark cancer cells. At molecular level, key evolutionarily conserved proteins that control establishment maintenance in various contexts are frequently altered cancer, but relevance these alterations oncogenic processes not always clear. Here, we summarize recent findings, shedding new light on involvement players development, discuss possibility harnessing cell changes to better predict, diagnose, cure cancers.

Language: Английский

Citations

17
Collective cell migration relies on PPP1R15-mediated regulation of the endoplasmic reticulum stress response DOI
Yujun Chen, Jocelyn A. McDonald

Current Biology, Journal Year: 2024, Volume and Issue: 34(7), P. 1390 - 1402.e4

Published: Feb. 29, 2024

Language: Английский

Citations

6
The Bright Side of Curcumin: A Narrative Review of Its Therapeutic Potential in Cancer Management DOI Open Access
Andrea Amaroli, Isabella Panfoli, Matteo Bozzo

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(14), P. 2580 - 2580

Published: July 18, 2024

Curcumin, a polyphenolic compound derived from Curcuma longa, exhibits significant therapeutic potential in cancer management. This review explores curcumin’s mechanisms of action, the challenges related to its bioavailability, and enhancement through modern technology approaches. Curcumin demonstrates strong antioxidant anti-inflammatory properties, contributing ability neutralize free radicals inhibit inflammatory mediators. Its anticancer effects are mediated by inducing apoptosis, inhibiting cell proliferation, interfering with tumor growth pathways various colon, pancreatic, breast cancers. However, clinical application is limited poor bioavailability due rapid metabolism low absorption. Novel delivery systems, such as curcumin-loaded hydrogels nanoparticles, have shown promise improving curcumin efficacy. Additionally, photodynamic therapy has emerged complementary approach, where light exposure enhances modulating molecular crucial for survival. Studies highlight that combining concentrations visible irradiation significantly boosts antitumor efficacy compared alone. The interaction cytochromes or drug transporters may play role altering pharmacokinetics conventional medications, which necessitates careful consideration settings. Future research should focus on optimizing understanding fully harness treatment.

Language: Английский

Citations

6
Cancer Bioenergetics and Tumor Microenvironments—Enhancing Chemotherapeutics and Targeting Resistant Niches through Nanosystems DOI Open Access
Aisha Farhana, Abdullah Alsrhani, Yusuf S. Khan

et al.

Cancers, Journal Year: 2023, Volume and Issue: 15(15), P. 3836 - 3836

Published: July 28, 2023

Cancer is an impending bottleneck in the advanced scientific workflow to achieve diagnostic, prognostic, and therapeutic success. Most cancers are refractory conventional diagnostic chemotherapeutics due their limited targetability, specificity, solubility, side effects. The inherent ability of each cancer evolve through various genetic epigenetic transformations metabolic reprogramming underlies limitations. Though tumor microenvironments (TMEs) quite well understood some cancers, microenvironment differs from other internal perturbations skew thereby impeding development appropriate diagnostics, drugs, vaccines, therapies. associated bioenergetics modulations regulate TME, angiogenesis, immune evasion, generation resistant niches progression, a thorough understanding crucial However, this remains missing element theranostics, necessitating modalities that can be adapted for diagnostics therapeutics. In challenging scenario, nanomaterials modular platforms TME achieving successful theranostics. Several nanoscale particles have been successfully researched animal models, few reached clinical trials, achieved Nanoparticles exhibit intrinsic capability interact with diverse biomolecules modulate functions. Furthermore, nanoparticles functionalized receptors, modulators, drugs facilitate specific targeting reduced toxicity. This review discusses current different theranostic nanosystems, synthesis, functionalization, targetability modulation bioenergetics, microenvironment. We highlight potential nanosystems enhanced chemotherapeutic success emphasizing questions remain unanswered.

Language: Английский

Citations

13
Stromal-Modulated Epithelial-to-Mesenchymal Transition in Cancer Cells DOI Creative Commons
Huda I. Atiya,

Grace Gorecki,

Geyon L. Garcia

et al.

Biomolecules, Journal Year: 2023, Volume and Issue: 13(11), P. 1604 - 1604

Published: Nov. 1, 2023

The ability of cancer cells to detach from the primary site and metastasize is main cause cancer- related death among all types. Epithelial-to-mesenchymal transition (EMT) first event metastatic cascade, resulting in loss cell–cell adhesion acquisition motile stem-like phenotypes. A critical modulator EMT stromal tumor microenvironment (TME), which can promote a mesenchymal phenotype through direct interaction with or changes broader microenvironment. In this review, we will explore role modulating cell EMT, particular emphasis on function stromal/stem (MSCs) activation EMT-inducing pathways, extra cellular matrix (ECM) remodeling, immune alteration, metabolic rewiring.

Language: Английский

Citations

13
The Role of Adipocytes Recruited as Part of Tumor Microenvironment in Promoting Colorectal Cancer Metastases DOI Open Access
Yunxia Ma, Miljana Nenkov,

Yuan Chen

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8352 - 8352

Published: July 30, 2024

Adipose tissue dysfunction, which is associated with an increased risk of colorectal cancer (CRC), a significant factor in the pathophysiology obesity. Obesity-related inflammation and extracellular matrix (ECM) remodeling promote metastasis (CRCM) by shaping tumor microenvironment (TME). When CRC occurs, metabolic symbiosis cells recruits adjacent adipocytes into TME to supply energy. Meanwhile, abundant immune cells, from adipose blood, are recruited TME, stimulated pro-inflammatory factors triggers chronic local TME. Dysregulated ECM proteins cell surface adhesion molecules enhance further increase contractibility between stromal promotes epithelial-mesenchymal transition (EMT). EMT increases migration invasion surrounding tissues or vessels accelerates CRCM. Colorectal symbiotic microbiota also plays important role promotion In this review, we provide its contributions CRC, special emphasis on adipocytes, macrophages, neutrophils, T ECM, gut progression their microenvironment. We highlight interactions potential therapeutic approaches target these interactions.

Language: Английский

Citations

5
The movement of mitochondria in breast cancer: internal motility and intercellular transfer of mitochondria DOI Creative Commons
Sarah Libring, Emily D. Berestesky, Cynthia A. Reinhart‐King

et al.

Clinical & Experimental Metastasis, Journal Year: 2024, Volume and Issue: 41(5), P. 567 - 587

Published: March 15, 2024

Abstract As a major energy source for cells, mitochondria are involved in cell growth and proliferation, as well migration, fate decisions, many other aspects of cellular function. Once thought to be irreparably defective, mitochondrial function cancer cells has found renewed interest, from suggested potential clinical biomarkers mitochondria-targeting therapies. Here, we will focus on the effect movement breast progression. Mitochondria move both within cell, such localize areas high energetic need, between where stroma have been shown donate their via multiple methods including tunneling nanotubes. The donation seen increase aggressiveness chemoresistance which increased recent efforts uncover mechanisms transfer. metabolism energetics gaining attention targets, better understanding implications required developing effective, targeted therapeutics patients.

Language: Английский

Citations

4
The Emerging Role of Ferroptosis in EBV-Associated Cancer: Implications for Cancer Therapy DOI Creative Commons
Shan He, Cheng Luo, Feng Shi

et al.

Biology, Journal Year: 2024, Volume and Issue: 13(7), P. 543 - 543

Published: July 18, 2024

Ferroptosis is a novel and iron-dependent form of programmed cell death, which has been implicated in the pathogenesis various human cancers. EBV well-recognized oncogenic virus that controls multiple signaling pathways within host cell, including ferroptosis signaling. Recent studies show inducing could be an efficient therapeutic strategy for EBV-associated tumors. This review will firstly describe mechanism ferroptosis, then summarize infection tumors, as well crosstalk between pathway, finally discuss role potential application ferroptosis-related reagents

Language: Английский

Citations

4
An alginate-cellulose based bioink mimics the viscoelastic features of the melanoma microenvironment and its influence on cell cycle and invasion DOI Creative Commons

C. F. Eckert,

Steven K. Schmidt, Jessica Faber

et al.

Bioprinting, Journal Year: 2025, Volume and Issue: unknown, P. e00384 - e00384

Published: Jan. 1, 2025

Language: Английский

Citations

0
Survival strategies of cancer cells: the role of macropinocytosis in nutrient acquisition, metabolic reprogramming, and therapeutic targeting DOI Creative Commons

Guoshuai Xu,

Qinghong Zhang, R. Holland Cheng

et al.

Autophagy, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Macropinocytosis is a nonselective form of endocytosis that allows cancer cells to largely take up the extracellular fluid and its contents, including nutrients, growth factors, etc. We first elaborate meticulously on process macropinocytosis. Only by thoroughly understanding this entire can we devise targeted strategies against it. then focus central role MTOR (mechanistic target rapamycin kinase) complex 1 (MTORC1) in regulating macropinocytosis, highlighting significance as key signaling hub where various pathways converge control nutrient uptake metabolic processes. The article covers comprehensive analysis literature molecular mechanisms governing initiation, maturation, recycling macropinosomes, with an emphasis how these processes are hijacked sustain their growth. Key discussions include potential therapeutic targeting such enhancing drug delivery via pathway, inhibiting macropinocytosis starve cells, blocking degradation inducing methuosis – cell death triggered excessive Targeting represents novel innovative approach could significantly advance treatment cancers rely pathway for survival. Through continuous research innovation, look forward developing more effective safer anti-cancer therapies will bring new hope patients.

Language: Английский

Citations

0