Seminal Plasma: Relevant for Fertility?

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(9), P. 4368 - 4368

Published: April 22, 2021

Seminal plasma (SP), the non-cellular component of semen, is a heterogeneous composite fluid built by secretions testis, epididymis and accessory sexual glands. Its composition, despite species-specific anatomical peculiarities, consistently contains inorganic ions, specific hormones, proteins peptides, including cytokines enzymes, cholesterol, DNA RNA-the latter often protected within epididymis- or prostate-derived extracellular vesicles. It beyond question that SP participates in diverse aspects sperm function pre-fertilization events. The also interacts with various compartments tubular genital tract, triggering changes gene prepares for an eventual successful pregnancy; thus, it ultimately modulates fertility. Despite these concepts, imperative to remember SP-free spermatozoa (epididymal washed ejaculated) are still fertile, so this review shall focus on differences between vivo roles following semen deposition female those regarding additions handled artificial reproduction, cryopreservation, from insemination vitro fertilization. This attempts, our own results model animal species, critically summarize current knowledge reproductive played components, particularly which increasingly affected infertility. ultimate goal reconcile delicate balance molecular concentration their concerted effects after temporal exposure vivo. We aim appraise functions relevance as diagnostic biomarkers value additives refine strategies, biotechnologies, livestock models humans.

Language: Английский

---

An updated review on the application of proteomics to explore sperm cryoinjury mechanisms in livestock animals

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 263, P. 107441 - 107441

Published: Feb. 21, 2024

Language: Английский

---

Reproductive physiology of the boar: What defines the potential fertility of an ejaculate?

Animal Reproduction Science, Journal Year: 2024, Volume and Issue: 269, P. 107476 - 107476

Published: April 18, 2024

Despite decades of research and handling semen for use in artificial insemination (AI) other assisted reproductive technologies, 5-10% selected boar sires are still considered sub-fertile, escaping current assessment methods sperm quality resilience to preservation. As end-product, the ejaculate (emitted spermatozoa sequentially exposed composite seminal plasma, SP) ought define homeostasis testes, epididymis, accessory sexual glands. Yet, linking findings production biology fertility is suboptimal. The present essay critically reviews how a fertile can help us diagnose both health handling, focusing on -available under development- identify suitable biomarkers cryotolerance fertility. Bulk SP, proteins microRNAs (miRNAs) have, albeit linked function after AI, failed enhance outcomes at commercial level, perhaps just being components complex functional pathway. Hence, focus now interaction sperm-SP, comparing vivo with ex vivo, regarding nano-sized lipid bilayer extracellular vesicles (sEVs) as priority. sEVs transport fragile molecules (lipids, proteins, nucleic acids) which, shielded from degradation, mediate cell-to-cell communication female internal genital tract. Such modulates essential processes, immunological tolerance. be harvested, characterized, stored, manipulated, e.g. used andrological diagnosis, selection breeders, alternatively additives improve cryosurvival

Language: Английский

---

Sperm metabolomics identifies freezability markers in Duroc, Landrace, and Large White boars

Theriogenology, Journal Year: 2025, Volume and Issue: unknown, P. 117395 - 117395

Published: March 1, 2025

Language: Английский

---

In Stallion Spermatozoa, Superoxide Dismutase (Cu–Zn) (SOD1) and the Aldo-Keto-Reductase Family 1 Member b (AKR1B1) Are the Proteins Most Significantly Reduced by Cryopreservation

Journal of Proteome Research, Journal Year: 2021, Volume and Issue: 20(5), P. 2435 - 2446

Published: March 3, 2021

Although cryopreservation is widely used in animal breeding, the technique still suboptimal. The population of spermatozoa surviving procedure experiences changes attributed to alteration their redox regulation. In order expand our knowledge regarding this particular aspect, proteome fresh and frozen thawed aliquots equine was studied identify proteins most severely affected by procedure. If regulation a major factor explaining cryodamage, participating should be principally affected. Using split sample design, 30 ejaculates from 10 different stallions were analyzed as spermatozoa, another aliquot same ejaculate sample. under both conditions using UHPLC-MS/MS bioinformatic analysis conducted discriminant variables between conditions. Data are available through ProteomeXchange Consortium with identifier PXD022236. significantly reduced Aldo-keto reductase family 1 member B (p = 2.2 × 10-17) Superoxide dismutase (Cu-Zn) 4.7 10-14). This first time that SOD1 has been identified discriminating variable analysis, where it one highly seen semen. finding strongly supports theory oxidative stress involved cryodamage suggests control target improve current procedures.

Language: Английский

---

Extracellular vesicles in seminal plasma: A safe and relevant tool to improve fertility in livestock?

Animal Reproduction Science, Journal Year: 2022, Volume and Issue: 244, P. 107051 - 107051

Published: Aug. 3, 2022

Seminal plasma (SP) is not a pre-requisite for pregnancy. Yet, this heterogeneous, composite SP has proven relevant fertility, as mediator cell-to-cell communication between producing cells, spermatozoa and the female internal genital tract, regulating complex reproductive processes. Bearing hormones, proteins, cytokines well nuclei acids in nano-sized lipid bilayer seminal extracellular vesicles (sEVs), concerts signaling to female. Signals influence timing of ovulation, sperm transport and, particularly, enable immune system balance her cryptic choice engage into pregnancy or reject an eventual fertilization. This essay, focusing on livestock general pigs particular, discusses intrinsic roles sEVs with regards homeostasis, while binding internalizing their cargo tract epithelia regulate functional activity. Since prior studies had inconclusive results using bulk single SP-contained free molecules, argumentation hereby provided increase current incipient research sEVs, where fragile molecules fertility are shielded from degradation during handling. EVs isolated can be used andrological diagnosis perhaps select breeders optimal fertility. Moreover, laboratory-uploaded specific even engineered nanodroplets additives extenders improve after artificial insemination (AI) biotechnologies.

Language: Английский

---

Characterization of freezability-associated metabolites in boar semen

Theriogenology, Journal Year: 2022, Volume and Issue: 196, P. 88 - 96

Published: Nov. 11, 2022

Language: Английский

---

Semen Protein CRISP3 Promotes Reproductive Performance of Boars through Immunomodulation

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2264 - 2264

Published: Feb. 14, 2024

Semen proteins play an important role in male reproductive performance and sperm fertilization ability can be used as potential biomarkers to evaluate fertility. The of cysteine-rich secretory protein 3 (CRISP3) reproduction remains unknown. This study aimed investigate the CRISP3 boars. Our results showed that content was significantly positively correlated with boar fertility, sow delivery rate, litter size. is highly expressed bulbourethral gland adult boars enriched seminal plasma. It localized post-acrosomal region head migrates anterior end tail after capacitation. recombinant did not affect motility cleavage but it downregulated mRNA expression inflammatory factors IL-α, IL-1β, IL-6 IL-α lipopolysaccharide (LPS)-induced RAW264.7 cells, indicating has immunomodulatory function. In conclusion, our suggests semen levels correlate performance, which may achieved by regulating immune responses female tract.

Language: Английский

---

Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality

Cell and Tissue Research, Journal Year: 2020, Volume and Issue: 383(2), P. 881 - 903

Published: Nov. 5, 2020

Language: Английский

---

Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine

Animal Reproduction, Journal Year: 2020, Volume and Issue: 17(3)

Published: Jan. 1, 2020

Seminal plasma (SP) supports not only sperm function but also the ability of spermatozoa to withstand biotechnological procedures as artificial insemination, freezing or sex sorting. Moreover, evidence has been provided that SP contains identifiable molecules which can act fertility biomarkers, and even improve output assisted reproductive technologies by acting modulators endometrial embryonic changes gene expression, thus affecting embryo development beyond horizon. In this overview, we discuss current knowledge composition SP, mainly proteins cytokines, their influence on semen basic procedures, such liquid storage cryopreservation. The role modulator molecular lead successful pregnancy will be discussed.

Language: Английский

---