Emerging trends in organ-on-a-chip systems for drug screening

Acta Pharmaceutica Sinica B, Journal Year: 2023, Volume and Issue: 13(6), P. 2483 - 2509

Published: Feb. 15, 2023

New drug discovery is under growing pressure to satisfy the demand from a wide range of domains, especially pharmaceutical industry and healthcare services. Assessment efficacy safety prior human clinical trials crucial part development, which deserves greater emphasis reduce cost time in discovery. Recent advances microfabrication tissue engineering have given rise organ-on-a-chip, an

Language: Английский

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Organ-on-a-chip meets artificial intelligence in drug evaluation

Theranostics, Journal Year: 2023, Volume and Issue: 13(13), P. 4526 - 4558

Published: Jan. 1, 2023

Drug evaluation has always been an important area of research in the pharmaceutical industry. However, animal welfare protection and other shortcomings traditional drug development models pose obstacles challenges to evaluation. Organ-on-a-chip (OoC) technology, which simulates human organs on a chip physiological environment functionality, with high fidelity reproduction organ-level physiology or pathophysiology, exhibits great promise for innovating pipeline. Meanwhile, advancement artificial intelligence (AI) provides more improvements design data processing OoCs. Here, we review current progress that made generate OoC platforms, how single multi-OoCs have used applications, including testing, disease modeling, personalized medicine. Moreover, discuss issues facing field, such as large reproducibility, point integration OoCs AI analysis automation, is benefit future Finally, look forward opportunities faced by coupling AI. In summary, advancements development, combinations AI, will eventually break state

Language: Английский

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Parkinson’s disease and gut microbiota: from clinical to mechanistic and therapeutic studies

Translational Neurodegeneration, Journal Year: 2023, Volume and Issue: 12(1)

Published: Dec. 15, 2023

Parkinson's disease (PD) is one of the most prevalent neurodegenerative diseases. The typical symptomatology PD includes motor symptoms; however, a range nonmotor symptoms, such as intestinal issues, usually occur before symptoms. Various microorganisms inhabiting gastrointestinal tract can profoundly influence physiopathology central nervous system through neurological, endocrine, and immune pathways involved in microbiota-gut-brain axis. In addition, extensive evidence suggests that gut microbiota strongly associated with PD. This review summarizes latest findings on microbial changes their clinical relevance, describes underlying mechanisms which bacteria may mediate PD, discusses correlations between microbes anti-PD drugs. this outlines status research therapies for future directions PD-gut research.

Language: Английский

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Biosensors integrated 3D organoid/organ-on-a-chip system: A real-time biomechanical, biophysical, and biochemical monitoring and characterization

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 231, P. 115285 - 115285

Published: April 7, 2023

Language: Английский

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3D Printing Techniques and Their Applications to Organ-on-a-Chip Platforms: A Systematic Review

Sensors, Journal Year: 2021, Volume and Issue: 21(9), P. 3304 - 3304

Published: May 10, 2021

Three-dimensional (3D) in vitro models, such as organ-on-a-chip platforms, are an emerging and effective technology that allows the replication of function tissues organs, bridging gap amid conventional models based on planar cell cultures or animals complex human system. Hence, they have been increasingly used for biomedical research, drug discovery personalized healthcare. A promising strategy their fabrication is 3D printing, a layer-by-layer process construction structures. In contrast, bioprinting, evolving biofabrication method, focuses accurate deposition hydrogel bioinks loaded with cells to construct tissue-engineered The purpose present work conduct systematic review (SR) published literature, according guidelines Preferred Reporting Items Systematic Reviews Meta-Analyses, providing source information evolution platforms obtained resorting printing bioprinting techniques. literature search, PubMed, Scopus, ScienceDirect databases were used, two authors independently performed study selection, data extraction. goal this SR highlight importance advantages using techniques obtaining also identify potential gaps future perspectives research field. Additionally, challenges integrating sensors organs-on-chip briefly investigated discussed.

Language: Английский

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In Vitro Strategies to Vascularize 3D Physiologically Relevant Models

Advanced Science, Journal Year: 2021, Volume and Issue: 8(19)

Published: Aug. 5, 2021

Abstract Vascularization of 3D models represents a major challenge tissue engineering and key prerequisite for their clinical industrial application. The use prevascularized built from dedicated materials could solve some the actual limitations, such as suboptimal integration bioconstructs within host tissue, would provide more in vivo‐like perfusable organ‐specific platforms. In last decade, fabrication vascularized physiologically relevant constructs has been attempted by numerous strategies, which are classified here microfluidic technology, coculture models, namely, spheroids organoids, biofabrication. this review, recent advancements prevascularization techniques increasing natural synthetic to build physiological discussed. Current drawbacks each future perspectives, translation toward clinics, pharmaceutical field, industry also presented. By combining complementary these envisioned be successfully used regenerative medicine drug development near future.

Language: Английский

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Recent developments in microfluidic technology for synthesis and toxicity-efficiency studies of biomedical nanomaterials

Materials Today Advances, Journal Year: 2022, Volume and Issue: 13, P. 100205 - 100205

Published: Jan. 6, 2022

Microfluidic technology is a relatively new field among the various conventional bulk techniques employed for nanomaterial synthesis. Nanoparticles (NPs) produced using such methods generally have poor surface features, morphology, uncontrolled size and broad distributions. NPs especially used in biomedical sector require degree of control over their size, polydispersity to improve functionality. To synthesize NPs, microfluidic devices are ideal as parameters mixing rates, flow volumes, multiphase reactions can be controlled on micro/nanoscale. There several strategies challenges synthesis nanoparticles from organic inorganic materials platform. The faced during design implementation these devices, large scale production interaction with soft lithography materials, which overcome by use technique conjugation microfluidics, parallelisation processes search fabrication materials. On other hand, organ-on-chip (OOC) analytical cell cutures present mimic an organ along its physio-chemical properties study cell-nanoparticle interaction. issues construction cultures within device affected As biocompatible being printed into more appropriate designs, utilized contain said reduce risk error analysis NP properties, efficiency toxicity. This review aims discuss recent developments well toxicity efficacy evaluations technology.

Language: Английский

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3D cancer models: One step closer to in vitro human studies

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: April 11, 2023

Cancer immunotherapy is the great breakthrough in cancer treatment as it displayed prolonged progression-free survival over conventional therapies, yet, to date, only a minority of patients. In order broad clinical applicability some roadblocks need be overcome, first among all lack preclinical models that faithfully depict local tumor microenvironment (TME), which known dramatically affect disease onset, progression and response therapy. this review, we provide reader with detailed overview current 3D developed mimick complexity dynamics TME, focus on understanding why TME major target anticancer We highlight advantages translational potentials spheroids, organoids immune Tumor-on-a-Chip modeling therapeutic response, while outlining pending challenges limitations. Thinking forward, possibility integrate know-hows micro-engineers, immunologists, pharmaceutical researchers bioinformaticians meet needs clinicians interested using these platforms high fidelity for patient-tailored drug discovery.

Language: Английский

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Vertical Organic Electrochemical Transistors and Electronics for Low Amplitude Micro‐Organ Signals

Advanced Science, Journal Year: 2022, Volume and Issue: 9(8)

Published: Jan. 22, 2022

Abstract Electrical signals are fundamental to key biological events such as brain activity, heartbeat, or vital hormone secretion. Their capture and analysis provide insight into cell organ physiology a number of bioelectronic medical devices aim improve signal acquisition. Organic electrochemical transistors (OECT) have proven their capacity neuronal cardiac with high fidelity amplification. Vertical PEDOT:PSS‐based OECTs (vOECTs) further enhance amplification device density but not been characterized in applications. An electronic board individually tuneable transistor biases overcomes fabrication induced heterogeneity metrics allows quantitative experiments. Careful exploration vOECT electric parameters defines voltage compatible reliable function experiments provides useful maximal transconductance values without influencing cellular generation propagation. This permits successful application monitoring micro‐organs prime importance diabetes, the endocrine pancreatic islets, which known for far smaller amplitudes compared neurons heart cells. Moreover, vOECTs single‐cell action potentials multicellular slow reflecting micro‐organ organizations well modulation by physiological stimulator glucose. opens possibility use new biomedical fields beyond classical

Language: Английский

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Advances and challenges in organ-on-chip technology: toward mimicking human physiology and disease in vitro

Medical & Biological Engineering & Computing, Journal Year: 2024, Volume and Issue: 62(7), P. 1925 - 1957

Published: March 4, 2024

Language: Английский

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