Journal of Controlled Release, Journal Year: 2024, Volume and Issue: 377, P. 54 - 80
Published: Nov. 17, 2024
Language: Английский
Trends in biotechnology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Language: Английский
Citations
25
Circulation Research, Journal Year: 2024, Volume and Issue: 135(1), P. 198 - 221
Published: June 20, 2024
From their humble discovery as cellular debris to cementing natural capacity transfer functional molecules between cells, the long-winded journey of extracellular vesicles (EVs) now stands at precipice a next-generation cell-free therapeutic tool revolutionize modern-day medicine. This perspective provides snapshot EVs emergence vibrant field biology and renaissance they usher in biomedical sciences agents for cardiovascular pathologies. Rapid development bioengineered is providing innovative opportunities overcome biological challenges such potency, cargo loading enhanced secretion, targeting circulation half-life, localized sustained delivery strategies, approaches enhance systemic circulation, uptake lysosomal escape, logistical hurdles encompassing scalability, cost, time. A multidisciplinary collaboration beyond extends chemistry, physics, biomaterials, nanotechnology, allowing rapid designer that are entering late-stage human clinical trials.
Language: Английский
Citations
19
Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)
Published: Feb. 24, 2025
Human umbilical cord mesenchymal stem cell-derived small extracellular vesicles (hucMSC-sEV) have recently garnered attention as a potential therapeutic approach for kidney diseases with anti-inflammatory effects. Infiltrated macrophages play an important role in facilitating tissue regeneration. However, the intricate regulatory effects of hucMSC-sEV on during cisplatin-induced acute injury (AKI) remain unknown. In this study, we uncovered that exhibited potent anti-inflammation and effectively inhibited polarization M1 phenotype macrophages. Mechanically, miRNA sequencing analysis qRT-PCR indicated novel miRNA, named miR-13896, was enriched hucMSC-sEV. When transfected miR-13896 mimic, displayed M2 elevated levels Arg1 IL-10, while inhibitor promoted phenotype. Furthermore, firstly established repressed Tradd expression by targeting its 3' untranslated region subsequently NF-κB signaling pathway Additionally, to improve effects, were engineered through electroporation, which resulted promoting macrophages, inhibiting inflammatory factors, enhancing repair. Conclusively, our findings provide insights into mechanisms underlying AKI, also highlighting electroporation promising strategy treating AKI.
Language: Английский
Citations
3
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6533 - 6533
Published: June 13, 2024
Extracellular vesicles (EVs) hold great promise for clinical application as new diagnostic and therapeutic modalities. This paper describes major GMP-based upstream downstream manufacturing processes EV large-scale production, also focusing on post-processing technologies such surface bioengineering uploading studies to yield novel EV-based diagnostics advanced therapy medicinal products. focuses the quality, safety, efficacy issues of bioengineered drug candidates before first-in-human studies. Because trials involving extracellular are global rise, this encompasses different registered clinical-trial register platforms, with varying levels advancement, highlighting growing interest in EV-related programs. Navigating regulatory affairs EVs poses real challenges, obtaining marketing authorization medicines remains complex due lack specific guidelines discusses state-of-the-art knowledge date products, further research needs safe reliable implementation tools settings. Post-marketing pharmacovigilance products is presented, mainly addressing topics risk assessment management.
Language: Английский
Citations
14
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: March 21, 2024
Abstract Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) have garnered extensive attention as natural product-based nanomedicines and potential drug delivery vehicles. However, the specific mechanism for regulating MSC-EVs secretion remains unclear. Here, we demonstrate that matrix (ECM) stiffness regulates of EVs by affecting MSCs' cargo sorting mechanically. Using multi-omics analysis, found a decrease in ECM impeded vesicular transport-related proteins autophagy-related lipids into MSC-EVs, impairing their subsequent uptake macrophages. Hence, with different behaviors can be produced changing culture substrates. This study provides new insights MSC-EV biology establishes connection between from biophysical perspective, providing basis rational design biomedical materials. Graphical
Language: Английский
Citations
10
International Journal of Oral Science, Journal Year: 2025, Volume and Issue: 17(1)
Published: Feb. 3, 2025
Abstract The oral and maxillofacial region is a highly complex area composed of multiple tissue types bears various critical functions the human body. Diseases in this pose significant diagnostic management challenges; therefore, exploring new strategies for early diagnosis, targeted treatment, reconstruction key to improving patient prognosis quality life. Extracellular vesicles are group heterogeneous lipid-bilayer membrane structures secreted by most cell types, including exosomes, microvesicles, apoptotic bodies. Present body fluids tissues, they act as messengers via transfer nucleic acids, proteins, metabolites recipient cells. To date, studies have revealed different roles extracellular physiological or pathological processes, well applications disease prognosis, treatment. importance specificity dental tissues indicate that derived from promising further research. This paper reviews published data on cells, fluids, regions, summarizes latest advances extensive sources, concludes with focus current research progress application prospects engineered exosomes science.
Language: Английский
Citations
1
Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)
Published: Feb. 4, 2025
Extracellular vesicles (EVs) secreted by mesenchymal stromal cells (MSCs) have been shown to provide significant protection against renal ischemia–reperfusion injury (IRI). Hypoxia has emerged as a promising strategy enhance the tissue repair capabilities of MSCs. However, specific effects hypoxia on MSCs and MSC-EVs, well their therapeutic potential in IRI, remain unclear. In this study, we investigated alterations occurring production MSC-EVs following pre-treatment, further explored key intrinsic mechanisms underlying hypoxic treatment IRI. Human umbilical cord were cultured under normoxic conditions. Proliferation related pathways measured, RNA sequencing was used detect changes transcriptional profile. from both conditions isolated characterized. vivo, localization assessed rat IRI model. Histological examinations conducted evaluate structure, proliferation, apoptosis kidney respectively. Renal function measuring serum creatinine blood urea nitrogen levels. vitro, measured tubular epithelial injured antimycin A. Protein analysis performed, depletion Glutathione S-Transferase Omega 1 (GSTO1) carried out verify its role alleviating injury. alters profile, promotes increases EVs. Hypoxia-pretreated demonstrated superior ability mitigate enhancing proliferation reducing vivo vitro. profiling EVs revealed an accumulation numerous anti-oxidative stress proteins, with GSTO1 being particularly prominent. Knockdown significantly reduced antioxidant MSC-EVs. generation enhances The effect induced is identified one most critical mechanisms. Our findings highlight that hypoxia-pretreated represent novel for
Language: Английский
Citations
1
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 25, 2025
Extracellular vesicles (EVs) are critical mediators of intercellular communication, carrying bioactive cargo and displaying diverse surface components that reflect their cellular origins functions. The EV surface, composed proteins, lipids, glycocalyx elements, plays a pivotal role in targeting recipient cells, mediating biological interactions, enabling selective delivery. This review comprehensively examined the molecular architecture surfaces, linking biogenesis to functional diversity, highlights therapeutic diagnostic potential diseases such as cancer cardiovascular disorders. Additionally, we explore emerging applications EVs, including machine-learning-assisted analysis, chemical integration, cross-system combinations. also discusses some key challenges clinical translation EV-related technologies.
Language: Английский
Citations
1
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: June 26, 2024
Abstract Background The use of stem cell-derived exosomes (Exos) as therapeutic vehicles is receiving increasing attention. Exosome administration has several advantages over cell transplantation, thus making promising candidates for large-scale clinical implementation and commercialization. However, exosome extraction purification efficiencies are relatively low, heterogeneity high due to differences in culture conditions viability. Therefore, this study, we investigated a priming procedure enhance the production effects from human umbilical cord mesenchymal cells (hucMSCs). After preconditioning hucMSCs with agonists/inhibitors that target Wnt/β-catenin pathway, assessed both efficacy optimized context diabetic wound healing, hoping provide safer, more stable effective option application. Results Wnt signalling pathway agonist CHIR99021 increased by 1.5-fold without causing obvious changes characteristics or size particles. Further studies showed promoted facilitating exocytosis. This process was partly mediated SNAP25. To further explore whether changed cargo loaded into its effects, performed proteomic transcriptomic analyses primed control hucMSCs. results significantly upregulated expression proteins associated migration healing. Animal experiments confirmed that, compared hucMSC-derived exosomes, CHIR99021-pretreated (CHIR-Exos) accelerated healing mice, enhanced local collagen deposition, angiogenesis, reduced chronic inflammation. Subsequent vitro CHIR-Exos migration, inhibiting oxidative stress-induced apoptosis, preventing cycle arrest. Conclusions secretion hucMSCs, which Notably, treatment also exosomal levels resulting acceleration All these suggested pretreatment not only but improved efficacy, providing Graphical
Language: Английский
Citations
4
Pharmaceutics, Journal Year: 2024, Volume and Issue: 16(10), P. 1306 - 1306
Published: Oct. 7, 2024
Biomimetic nanoparticles (BMNPs) are innovative nanovehicles that replicate the properties of naturally occurring extracellular vesicles, facilitating highly efficient drug delivery across biological barriers to target organs and tissues while ensuring maximal biocompatibility minimal-to-no toxicity. BMNPs can be utilized for therapeutic payloads imparting novel other nanotechnologies based on organic inorganic materials. The application specifically modified membranes coating has potential enhance their efficacy biocompatibility, presenting a promising pathway advancement technologies. This manuscript is grounded in fundamentals biomimetic technologies, offering comprehensive overview analytical perspective preparation functionalization BMNPs, which include cell membrane-coated (CMCNPs), artificial cell-derived vesicles (ACDVs), fully synthetic (fSVs). review examines both "top-down" "bottom-up" approaches nanoparticle preparation, with particular focus techniques such as membrane coating, cargo loading, microfluidic fabrication. Additionally, it addresses technological challenges solutions associated large-scale production clinical related
Language: Английский
Citations
4