Advanced Drug Delivery Reviews, Journal Year: 2024, Volume and Issue: 217, P. 115486 - 115486
Published: Dec. 10, 2024
Language: Английский
Nature Medicine, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 3, 2025
Language: Английский
Citations
9
Drug Discovery Today, Journal Year: 2024, Volume and Issue: 29(4), P. 103924 - 103924
Published: Feb. 22, 2024
Language: Английский
Citations
10
Gut, Journal Year: 2025, Volume and Issue: unknown, P. gutjnl - 333885
Published: March 3, 2025
Background To treat liver failure, three-dimensional (3D) bioprinting is a promising technology used to construct hepatic tissue models. However, current research on of models primarily relies conventional single-cell-based bioprinting, where individual functional hepatocytes are dispersed and isolated within hydrogels, leading insufficient treatment outcomes due inadequate cell functionality. Objective Here, we aim bioprint model using hepatocyte organoids (HOs) evaluate its liver-specific functions in vitro vivo . Design Human chemically induced pluripotent stem cells (hCiPSCs) were as robust non-genome-integrative source produce highly viable HOs (hCiPSC-HOs). An oxygen-permeable microwell device was enhance oxygen supply, ensuring high viability promoting hCiPSC-HOs maturation. maintain the long-term biofunction hCiPSC-HOs, spheroid-based employed (3DP-HOs). 3DP-HOs intraperitoneally implanted mice with failure. Results demonstrated enhanced when compared fabricated exhibited gene profiles closely resembling while maintaining Moreover, implantation significantly improved survival CCl 4 -induced acute-on-chronic failure also Fah−/− reduced injury, inflammation fibrosis indices regeneration expression. Conclusion Our bioprinted exhibits remarkable therapeutic efficacy for holds great potential clinical field regenerative medicine.
Language: Английский
Citations
1
Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1066 - 1066
Published: Aug. 26, 2024
Skin aging is influenced by intrinsic and extrinsic factors that progressively impair skin functionality over time. Investigating the process requires thorough research using innovative technologies. This review explores use of in vitro human 3D culture models, serving as valuable alternatives to animal ones, research. The aim highlight benefits necessity improving methodology analyzing molecular mechanisms underlying aging. Traditional 2D including monolayers keratinocytes, fibroblasts, or melanocytes, even if providing cost-effective straightforward methods study critical processes such extracellular matrix degradation, pigmentation, effects secretome on cells, fail replicate complex tissue architecture with its intricated interactions. Advanced models (organoid cultures, “skin-on-chip” technologies, reconstructed skin, bioprinting) considerably enhance physiological relevance, enabling a more accurate representation peculiar features. By reporting advantages limitations this highlights importance advanced systems develop practical anti-aging preventive reparative approaches improve translational field. Further exploration these technologies will provide new opportunities for previously unexplored knowledge
Language: Английский
Citations
8
Small Methods, Journal Year: 2024, Volume and Issue: unknown
Published: May 19, 2024
Organ-on-a-chip, also known as "tissue chip," is an advanced platform based on microfluidic systems for constructing miniature organ models in vitro. They can replicate the complex physiological and pathological responses of human organs. In recent years, development bone joint-on-chip platforms aims to simulate processes occurring bones joints, including cell-cell interactions, interplay various biochemical factors, effects mechanical stimuli, intricate connections between multiple future, will integrate advantages disciplines, bringing more possibilities exploring disease mechanisms, drug screening, personalized medicine. This review explores construction application Organ-on-a-chip technology joint research, proposes a modular concept, discusses new opportunities future challenges platforms.
Language: Английский
Citations
6
Biofabrication, Journal Year: 2024, Volume and Issue: 16(3), P. 035026 - 035026
Published: May 29, 2024
The evaluation of anti-tumor drugs is critical for their development and clinical guidance. Tumor organoid models are gaining increased attention due to ability better mimic real tumor tissues, as well lower time economic costs, which makes up the shortcomings cell lines xenograft models. However, current cultures based on Matrigel have limitations in matching with high-throughput engineering methods slow gelation low mechanical strength. Here, we present a novel composite bioink culturing colorectal cancer organoids that provides an environment close tissue growth conditions exhibits excellent photocrosslinking properties rapid gel formation. Most importantly, viability after printing was high 97%, also kept multicellular polar structures consistent traditional culture Matrigel. Using 3D bioprinting this loaded organoids, demonstrated feasibility drug model by validating it clinically used treatment drugs. Our results suggested could effectively cultivate using bioprinting, had potential replace less reliable manual operations promoting application
Language: Английский
Citations
6
Cells, Journal Year: 2024, Volume and Issue: 13(19), P. 1638 - 1638
Published: Oct. 1, 2024
The 3D bioprinting technique has made enormous progress in tissue engineering, regenerative medicine and research into diseases such as cancer. Apart from individual cells, a collection of organoids, can be printed combination with various hydrogels. It hypothesized that will even become promising tool for mechanobiological analyses organoids their matrix environments highly defined precisely structured environments, which the mechanical properties cell environment individually adjusted. Mechanical obstacles or bead markers integrated bioprinted samples to analyze deformations forces within these constructs, perform biophysical analysis complex systems, are still not standard techniques. review highlights advances 4D printing technologies integrating cues so next step detailed key future directions organoid generation development disease model regeneration drug testing perspective. Finally, hydrogels, pure natural synthetic hydrogels mixtures, organoid–cell co-cultures, organ-on-a-chip systems organoid-organ-on-a chip combinations introduces use assembloids determine mutual interactions different types cell–matrix interferences specific biological environments.
Language: Английский
Citations
6
Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)
Published: Nov. 8, 2024
The complexity of tumors and the challenges associated with treatment often stem from limitations existing models in accurately replicating authentic tumors. Recently, organoid technology has emerged as an innovative platform for tumor research. This bioengineering approach enables researchers to simulate, vitro, interactions between their microenvironment, thereby enhancing intricate interplay cells surroundings. Organoids also integrate multidimensional data, providing a novel paradigm understanding development progression while facilitating precision therapy. Furthermore, advancements imaging genetic editing techniques have significantly augmented potential organoids review explores application more precise simulations its specific contributions cancer research advancements. Additionally, we discuss evolving trends developing comprehensive utilizing technology.
Language: Английский
Citations
6
Medical Review, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 13, 2025
Abstract Current two-dimensional (2D) cell models for effective drug screening suffer from significant limitations imposed by the lack of realism in physiological environment. Three-dimensional (3D) organoids hold immense potential mimicking key functions human organs overcoming traditional 2D models. However, current techniques preparation 3D had reproducibility, scalability, and ability to closely replicate complex microenvironment found vivo . Additionally, culture systems often involve lengthy labor-intensive processes that hinder high-throughput applications necessary a large-scale screening. Advancements bioprinting technologies offer promising solutions these challenges enabling precise spatial control over placement material composition, thereby facilitating creation more physiologically relevant than techniques. This review provides comprehensive summary recent advances creating models, which begins with an introduction different types (especially focus on volumetric (VBP) technique), followed overview bioinks utilized bioprinting. Moreover, we also introduce disease efficiency evaluation regenerative medicine. Finally, possible strategies development clinical translation are concluded.
Language: Английский
Citations
0
ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown
Published: March 2, 2025
Mesenchymal stem cells (MSCs) play a critical role in cell therapy due to their tissue-mimicking abilities. However, conventional 2D culture conditions often lead the loss of native hypoxic niche, potentially limiting therapeutic efficacy. 3D bioprinting offers method recreate intricate biological environments by integrating with extracellular matrices. Therefore, it is essential adapt printing techniques accurately replicate MSCs' ecological facilitating integration technology into clinical applications. In this study, we optimized capabilities using performed cellular aggregates (PCA) method. We observed that printed matrix creates microenvironment, resulting significant increase level production several paracrine signaling molecules and immunomodulatory factors MSCs. Furthermore, MSCs exhibited enhanced stemness proliferative capacity early stages culture. RNA-seq analysis revealed these changes behavior were associated environment created during procedure By optimizing bioink composition parameters, successfully simulated vivo leading notable improvements MSC characteristics capacity. RNA sequencing further confirmed activation hypoxia pathways, which are crucial for properties. These findings offer valuable insights leveraging MSC-based therapies regenerative medicine.
Language: Английский
Citations
0