In Vitro Persistence Level Reflects In Vivo Antibiotic Survival of Natural Pseudomonas aeruginosa Isolates in a Murine Lung Infection Model DOI Creative Commons
Laure Verstraete, Juliana Aizawa, Matthias Govaerts

et al.

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(3)

Published: May 4, 2023

Clinicians are increasingly confronted with the limitations of antibiotics to clear bacterial infections in patients. It has long been assumed that only antibiotic resistance plays a pivotal role this phenomenon. Indeed, worldwide emergence is considered one major health threats 21st century. However, presence persister cells also significant influence on treatment outcomes. These antibiotic-tolerant present every population and result phenotypic switching normal, antibiotic-sensitive cells. Persister complicate current therapies contribute development resistance. In past, extensive research performed investigate persistence laboratory settings; however, tolerance under conditions mimic clinical setting remain poorly understood. study, we optimized mouse model for lung opportunistic pathogen Pseudomonas aeruginosa. model, mice intratracheally infected P. aeruginosa embedded seaweed alginate beads subsequently treated tobramycin via nasal droplets. A diverse panel 18 strains originating from environmental, human, animal sources was selected assess survival model. Survival levels were positively correlated determined time-kill assays, common method study laboratory. We showed comparable thus classical assays indicative setting. The enables us test potential antipersister relevant settings. IMPORTANCE importance targeting becoming more evident, as these underlie relapsing development. Here, studied clinically pathogen, six ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, aeruginosa, Enterobacter spp.), which threats. mostly known cause chronic cystic fibrosis mimicked conditions. shown natural isolates measured vitro. results not validate use our techniques but open opportunities new mechanisms or evaluate strategies vivo.

Language: Английский

Facile synthesis of N-doped carbon nanorods for antibiotics degradation via PMS activation: Mechanism insight and biotoxicity assessment DOI
Fang Chen, Jiaying Yan, Yanlan Wang

et al.

Separation and Purification Technology, Journal Year: 2024, Volume and Issue: 340, P. 126849 - 126849

Published: Feb. 20, 2024

Language: Английский

Citations

34
Phage Paride can kill dormant, antibiotic-tolerant cells of Pseudomonas aeruginosa by direct lytic replication DOI Creative Commons
Enea Maffei,

Anne-Kathrin Woischnig,

Marco Burkolter

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 2, 2024

Abstract Bacteriophages are ubiquitous viral predators that have primarily been studied using fast-growing laboratory cultures of their bacterial hosts. However, microbial life in nature is mostly a slow- or non-growing, dormant state. Here, we show diverse phages can infect deep-dormant bacteria and suspend replication until the host resuscitates (“hibernation”). newly isolated Pseudomonas aeruginosa phage, named Paride, directly replicate induce lysis While non-growing notoriously tolerant to antibiotic drugs, combination with Paride enables carbapenem meropenem eradicate vitro reduce resilient infection tissue cage implant mice. Our work might inspire new treatments for persistent infections and, more broadly, highlights two strategies (hibernation direct replication) will guide future studies on phage-host interactions.

Language: Английский

Citations

32
Environmental, mechanistic and evolutionary landscape of antibiotic persistence DOI
Celien Bollen, Elen Louwagie, Natalie Verstraeten

et al.

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(8)

Published: July 3, 2023

Language: Английский

Citations

28
Biofilm antimicrobial susceptibility through an experimental evolutionary lens DOI Creative Commons
Tom Coenye, Mona Bové, Thomas Bjarnsholt

et al.

npj Biofilms and Microbiomes, Journal Year: 2022, Volume and Issue: 8(1)

Published: Oct. 18, 2022

Abstract Experimental evolution experiments in which bacterial populations are repeatedly exposed to an antimicrobial treatment, and examination of the genotype phenotype resulting evolved bacteria, can help shed light on mechanisms behind reduced susceptibility. In this review we present overview why it is important include biofilms experimental evolution, approaches available study what has taught us about tolerance resistance biofilms. Finally, emerging consensus view biofilm susceptibility supported by data obtained during studies.

Language: Английский

Citations

35
Role of (p)ppGpp in antibiotic resistance, tolerance, persistence and survival in Firmicutes DOI Creative Commons
Andrea Salzer, Christiane Wolz

microLife, Journal Year: 2023, Volume and Issue: 4

Published: Jan. 1, 2023

The stringent response and its signalling nucleotides, pppGpp ppGpp, have been the subject of intense research since discovery (p)ppGpp in 1969. Recent studies revealed that downstream events follow accumulation vary among species. Consequently, as initially characterized

Language: Английский

Citations

22
Controlling biofilm and virulence properties of Gram-positive bacteria by targeting wall teichoic acid and lipoteichoic acid DOI
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum

et al.

International Journal of Antimicrobial Agents, Journal Year: 2023, Volume and Issue: 62(4), P. 106941 - 106941

Published: Aug. 2, 2023

Language: Английский

Citations

19
pH-responsive polymeric nanomaterials for the treatment of oral biofilm infections DOI
Geum-Jae Jeong, Muzamil Ahmad Rather, Fazlurrahman Khan

et al.

Colloids and Surfaces B Biointerfaces, Journal Year: 2023, Volume and Issue: 234, P. 113727 - 113727

Published: Dec. 26, 2023

Language: Английский

Citations

18
New Strategies to Kill Metabolically-Dormant Cells Directly Bypassing the Need for Active Cellular Processes DOI Creative Commons
Karolina Stojowska‐Swędrzyńska, Dorota Kuczyńska‐Wiśnik, Ewa Laskowska

et al.

Antibiotics, Journal Year: 2023, Volume and Issue: 12(6), P. 1044 - 1044

Published: June 12, 2023

Antibiotic therapy failure is often caused by the presence of persister cells, which are metabolically-dormant bacteria capable surviving exposure to antimicrobials. Under favorable conditions, persisters can resume growth leading recurrent infections. Moreover, several studies have indicated that may promote evolution antimicrobial resistance and facilitate selection specific resistant mutants; therefore, in light increasing numbers multidrug-resistant infections worldwide, developing efficient strategies against dormant cells paramount importance. In this review, we present discuss efficacy various agents whose activity independent metabolic status as they target cell envelope structures. Since biofilm-environment for formation subpopulations, anti-persister should also include destroy biofilm matrix or inhibit development. This article reviews examples selected wall hydrolases, polysaccharide depolymerases peptides. Their combination with standard antibiotics seems be most promising approach combating persistent

Language: Английский

Citations

15
Antibiotic persistence of intracellular Brucella abortus DOI Creative Commons

Selma Mode,

Maren Ketterer, Maxime Québatte

et al.

PLoS neglected tropical diseases, Journal Year: 2022, Volume and Issue: 16(7), P. e0010635 - e0010635

Published: July 26, 2022

Background Human brucellosis caused by the facultative intracellular pathogen Brucella spp. is an endemic bacterial zoonosis manifesting as acute or chronic infections with high morbidity. Treatment typically involves a combination therapy of two antibiotics for several weeks to months, but despite this harsh treatment relapses occur at rate 5–15%. Although poor compliance and reinfection may account fraction observed relapse cases, it apparent that properties infectious agent itself play decisive role in phenomenon. Methodology/Principal findings We used B . abortus carrying dual reporter macrophage infection model gain better understanding efficacy recommended therapies cellulo For we automated fluorescent microscopy prime read-out developed specific CellProfiler pipelines score infected macrophages population single cell level. Combining constitutive induced reporters classical CFU determination, quantified protective nature lifestyle various ability persist antibiotic treatments. Conclusion/Significance demonstrate concentrations fails fully prevent growth persistence , which be explained niche(s). Moreover, show presence bona fide persisters upon treatment, are metabolically active retain full potential, therefore constituting plausible reservoir relapse. In conclusion, our results highlight need extend spectrum models test new antimicrobial reflect vivo environment, develop therapeutic approaches targeting persister subpopulation.

Language: Английский

Citations

19
Nitrogen-doped porous carbon derived from graphite of solid waste for activating peroxymonosulfate to degradation tetracycline DOI

Zhifei Meng,

Ruixing Mo,

Qian Wang

et al.

Colloids and Surfaces A Physicochemical and Engineering Aspects, Journal Year: 2023, Volume and Issue: 662, P. 130984 - 130984

Published: Jan. 18, 2023

Language: Английский

Citations

11