bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 14, 2023
Human
Papillomaviruses
(HPVs)
are
associated
with
around
5-10%
of
human
cancer,
notably
nearly
99%
cervical
cancer.
The
mechanisms
HPV
interacts
stratified
epithelium
(differentiated
layers)
during
the
viral
life
cycle,
and
oncogenesis
remain
unclear.
In
this
study,
we
used
single-cell
transcriptome
analysis
to
study
gene
host
cell
differentiation-associated
heterogeneity
HPV-positive
cancer
tissue.
We
examined
HPV16
genes
-
E1,
E6,
E7,
found
they
expressed
differently
across
nine
epithelial
clusters.
that
three
clusters
had
highest
proportion
cells
(33.6%,
37.5%,
32.4%,
respectively),
while
two
exhibited
lowest
proportions
(7.21%
5.63%,
respectively).
Notably,
cluster
most
deviated
significantly
from
normal
layer
markers,
exhibiting
functional
altered
structuring,
indicating
significant
molecular
existed
in
tissues
these
unique/different
signatures
compared
cells.
These
cells,
HPV-negative,
showed
different
expressions
related
extracellular
matrix,
adhesion,
proliferation,
apoptosis.
Further,
oncogenes
E6
E7
appeared
modify
function
via
distinct
pathways,
thus
contributing
progression.
investigated
transcripts
a
novel
viewpoint
focusing
on
heterogeneity.
Our
results
indicated
varied
expression
oncogenes,
biological
insights
critical
field
study.
Biomarker Research,
Journal Year:
2024,
Volume and Issue:
12(1)
Published: Sept. 27, 2024
Abstract
Cells,
as
the
fundamental
units
of
life,
contain
multidimensional
spatiotemporal
information.
Single-cell
RNA
sequencing
(scRNA-seq)
is
revolutionizing
biomedical
science
by
analyzing
cellular
state
and
intercellular
heterogeneity.
Undoubtedly,
single-cell
transcriptomics
has
emerged
one
most
vibrant
research
fields
today.
With
optimization
innovation
technologies,
intricate
details
concealed
within
cells
are
gradually
unveiled.
The
combination
scRNA-seq
other
multi-omics
at
forefront
field.
This
involves
simultaneously
measuring
various
omics
data
individual
cells,
expanding
our
understanding
across
a
broader
spectrum
dimensions.
precisely
captures
aspects
transcriptomes,
immune
repertoire,
spatial
information,
temporal
epitopes,
in
diverse
contexts.
In
addition
to
depicting
cell
atlas
normal
or
diseased
tissues,
it
also
provides
cornerstone
for
studying
differentiation
development
patterns,
disease
heterogeneity,
drug
resistance
mechanisms,
treatment
strategies.
Herein,
we
review
traditional
technologies
outline
latest
advancements
multi-omics.
We
summarize
current
status
challenges
applying
biological
clinical
applications.
Finally,
discuss
limitations
potential
strategies
address
them.
npj Biofilms and Microbiomes,
Journal Year:
2025,
Volume and Issue:
11(1)
Published: Jan. 2, 2025
Emerging
evidence
indicates
that
oral
microbes
are
closely
related
to
gastric
and
lesions,
including
atrophy,
intestinal
metaplasia
cancer
(GC).
Helicobacter
pylori
is
a
key
pathogen
involved
in
GC.
However,
the
increasing
prevalence
of
H.
pylori-negative
GC
dysbiosis
patients
emphasize
potential
role
other
microbial
factors.
In
this
review,
we
discussed
current
about
relationship
between
oral–gastric
axis
health.
Epidemiologic
poor
hygiene
greater
risk.
Multiple
oral-associated
enriched
stomach
patients.
Once
colonizing
stomach,
Streptococcus
anginosus
Prevotella
melaninogenica,
inflammation
or
carcinogenesis.
Microbial
metabolites
such
as
lactate,
nitrite,
acetaldehyde
promote
malignant
transformation.
The
checkpoint
transmission
digestive
tract,
great
importance
since
link
diseases
has
been
emphasized.
Still,
new
technologies
standardized
metrics
necessary
identify
pathogenetic
for
core
microbiota,
interactions,
richness,
colonization,
location
effect
(CIRCLE).
future,
could
be
candidates
noninvasive
indicators
predict
diseases.
Molecular Cancer,
Journal Year:
2025,
Volume and Issue:
24(1)
Published: Jan. 15, 2025
The
polymorphic
microbiome
is
considered
a
new
hallmark
of
cancer.
Advances
in
High-Throughput
Sequencing
have
fostered
rapid
developments
research.
interaction
between
cancer
cells,
immune
and
microbiota
defined
as
the
immuno-oncology
(IOM)
axis.
Fungal
microbes
(the
mycobiome),
although
representing
only
∼
0.1-1%
microbiome,
are
critical
immunologically
active
component
tumor
microbiome.
Accumulating
evidence
suggests
possible
involvement
commensal
pathogenic
fungi
initiation,
progression,
treatment
responsiveness.
tumor-associated
mycobiome
mainly
consists
gut
mycobiome,
oral
intratumoral
mycobiome.
However,
role
remains
poorly
understood,
diversity
complexity
analytical
methods
make
it
challenging
to
access
this
field.
This
review
aims
elucidate
causal
complicit
roles
development
progression
while
highlighting
issues
that
need
be
addressed
executing
such
We
systematically
summarize
advantages
limitations
current
fungal
detection
analysis
methods.
enumerate
integrate
these
recent
findings
into
our
understanding
accompanied
by
prospect
novel
exhilarating
clinical
implications.
Annual Review of Physiology,
Journal Year:
2024,
Volume and Issue:
86(1), P. 453 - 478
Published: Feb. 12, 2024
Studies
in
preclinical
models
support
that
the
gut
microbiota
play
a
critical
role
development
and
progression
of
colorectal
cancer
(CRC).
Specific
microbial
species
their
corresponding
virulence
factors
or
associated
small
molecules
can
contribute
to
CRC
either
via
direct
effects
on
neoplastic
transformation
epithelial
cells
through
interactions
with
host
immune
system.
Induction
DNA
damage,
activation
Wnt/β-catenin
NF-κB
proinflammatory
pathways,
alteration
nutrient's
availability
metabolic
activity
are
main
mechanisms
by
which
CRC.
Within
tumor
microenvironment,
alter
recruitment,
activation,
function
various
cells,
such
as
T
macrophages,
dendritic
cells.
Additionally,
shape
composition
cancer-associated
fibroblasts
extracellular
matrix
components,
fashioning
an
immunosuppressive
pro-tumorigenic
niche
for
Understanding
complex
interplay
between
tumorigenesis
provide
therapeutic
opportunities
prevention
treatment
iScience,
Journal Year:
2024,
Volume and Issue:
27(6), P. 109893 - 109893
Published: May 3, 2024
Recent
advances
in
cancer
research
have
unveiled
a
significant
yet
previously
underappreciated
aspect
of
oncology:
the
presence
and
role
intratumoral
microbiota.
These
microbial
residents,
encompassing
bacteria,
fungi,
viruses
within
tumor
tissues,
been
found
to
exert
considerable
influence
on
development,
progression,
efficacy
therapeutic
interventions.
This
review
aims
synthesize
these
groundbreaking
discoveries,
providing
an
integrated
overview
identification,
characterization,
functional
roles
microbiota
biology.
We
focus
elucidating
complex
interactions
between
microorganisms
microenvironment,
highlighting
their
potential
as
novel
biomarkers
targets.
The
purpose
this
is
offer
comprehensive
understanding
dimension
cancer,
paving
way
for
innovative
approaches
diagnosis
treatment.
Acta Pharmaceutica Sinica B,
Journal Year:
2023,
Volume and Issue:
14(4), P. 1560 - 1591
Published: Nov. 15, 2023
Intratumoral
microbiota
has
become
research
hotspots,
and
emerges
as
a
non-negligent
new
component
of
tumor
microenvironments
(TME),
due
to
its
powerful
influence
on
initiation,
metastasis,
immunosurveillance
prognosis
despite
in
low-biomass.
The
accumulations
microbes,
their
related
components
metabolites
within
tissues,
endow
TME
with
additional
pluralistic
features
which
are
distinct
from
the
conventional
one.
Therefore,
it's
definitely
necessary
comprehensively
delineate
sophisticated
landscapes
microbe
microenvironment,
well
functions
underlying
mechanisms.
Herein,
this
review,
we
focused
fields
including
heterogeneity
intratumor
different
types
tumors,
controversial
roles
intratumoral
microbiota,
basic
microenvironment
(
