Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based on Quorum Sensing (QS) Signal-Response System and Innovative Non-Antibiotic Strategies for Their Elimination

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2655 - 2655

Published: Feb. 24, 2024

One of the key mechanisms enabling bacterial cells to create biofilms and regulate crucial life functions in a global highly synchronized way is communication system called quorum sensing (QS). QS cell-to-cell process that depends on population density mediated by small signalling molecules autoinducers (AIs). In bacteria, controls biofilm formation through regulation gene expression involved extracellular polymeric matrix (EPS) synthesis, virulence factor production, stress tolerance metabolic adaptation. Forming one antimicrobial resistance (AMR). A common feature human pathogens ability form biofilm, which poses serious medical issue due their high susceptibility traditional antibiotics. Because associated with formation, there belief inhibition activity quenching (QQ) may provide alternative therapeutic methods for treating microbial infections. This review summarises recent progress research, focusing biofilms, especially those formed pathogenic become resistant antibiotic treatment. Subsequently, potential approach highlighting innovative non-antibiotic strategies control AMR bacteria has been discussed.

Language: Английский

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The Potential Health Benefits of Gallic Acid: Therapeutic and Food Applications

Antioxidants, Journal Year: 2024, Volume and Issue: 13(8), P. 1001 - 1001

Published: Aug. 18, 2024

Gallic acid (GA), a phenolic found in fruits and vegetables, has been consumed by humans for centuries. Its extensive health benefits, such as antimicrobial, antioxidant, anticancer, anti-inflammatory, antiviral properties, have well-documented. GA's potent antioxidant capabilities enable it to neutralize free radicals, reduce oxidative stress, protect cells from damage. Additionally, GA exerts anti-inflammatory effects inhibiting inflammatory cytokines enzymes, making potential therapeutic agent diseases. It also demonstrates anticancer properties cancer cell growth promoting apoptosis. Furthermore, offers cardiovascular lowering blood pressure, decreasing cholesterol, enhancing endothelial function, which may aid the prevention management of This review covers chemical structure, sources, identification quantification methods, biological GA, along with its applications food. As research progresses, future appears promising, uses functional foods, pharmaceuticals, nutraceuticals aimed at improving overall preventing disease. However, ongoing innovation are necessary fully understand address current challenges, establish mainstay nutritional interventions.

Language: Английский

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The stress of bioactive compounds on microbes in anaerobic digestion of food waste and mitigation strategies: A critical review

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 493, P. 152746 - 152746

Published: June 1, 2024

Language: Английский

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Conserved patterns of sequence diversification provide insight into the evolution of two-component systems in Enterobacteriaceae

Microbial Genomics, Journal Year: 2024, Volume and Issue: 10(3)

Published: March 19, 2024

Two-component regulatory systems (TCSs) are a major mechanism used by bacteria to sense and respond their environments. Many of the same TCSs biologically diverse organisms with different needs, suggesting that functions TCS must evolve. To explore this topic, we analysed amino acid sequence divergence patterns large set broadly conserved across branches Enterobacteriaceae , family Gram-negative includes biomedically important genera such as Salmonella Escherichia Klebsiella others. Our analysis revealed trends in how sequences change proteins or functional domains TCS, lineages. Based on these trends, identified individual exhibit atypical evolutionary patterns. We observed relative extent which given varies lineages is generally well conserved, unveiling hierarchy conservation EnvZ/OmpR most TCS. provide evidence that, for divergent analysed, PmrA/PmrB, alleles were horizontally acquired family, PmrA/PmrB variants have highly signal-sensing domains. Collectively, study sheds light evolve, serves compendium diverged over course evolution.

Language: Английский

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Halotolerant Enterobacter asburiae A103 isolated from the halophyte Salix linearistipularis: genomic analysis and growth-promoting effects on Medicago sativa under alkali stress

Microbiological Research, Journal Year: 2024, Volume and Issue: 289, P. 127909 - 127909

Published: Sept. 19, 2024

Language: Английский

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γ Irradiation Alters the Staphylococcus aureus Proteome and Enhances Pathogenicity

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

Staphylococcus aureus (S. aureus) infection has become one of the most common and severe complications among cancer patients. The impact γ radiation from radiotherapy on S. aureus's growth virulence is not yet fully understood. In this study, was exposed to at a dose 100 Gy, its descendants were cultured under normal conditions. Proteome alternations unirradiated, irradiated, irradiated identified by using data-independent acquisition (DIA) proteomic technology. To investigate consequences proteome induced irradiation in aureus, functional enrichment analysis, pathway protein–protein interaction network analysis performed. Differentially expressed proteins (DEPs) primarily enriched lipoteichoic acid biosynthesis, infection, two-component system, cationic antimicrobial peptide resistance, suggesting an enhanced ability. A strong ability typically associated with increased biofilm formation. Both study assay indicate that enhances likely resulting pathogenicity.

Language: Английский

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Bacteria‐responsive transformable peptide‐based nanomaterials inspired by human α‐defensin 6 for labeling and entrapping pathogenic bacteria

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Antimicrobial resistance caused by overuse of antibiotics has promoted the demand for effective antibacterial materials. However, development existing strategies mostly focuses on direct sterilization, which may lead to flora imbalance and drug resistance. Here, a series peptide‐based aggregation‐induced emssion nanomaterials (PBANs) with multiple structural domains were designed mimicking self‐assembly human α‐defensin 6. Specifically, PBANs self‐assemble form nanoparticles in physiological environments situ transform into nanofibers bacterial surfaces through receptor‐ligand interactions infected microenvironments, resulting enhanced fluorescence signal activation functions, while labeling entrapping bacteria. Different from traditional that directly kill pathogenic microorganisms, can inhibit motility invasion host system physical barriers affecting energy metabolism pathways. In addition, further recruit macrophages infection site engulf entrapped bacteria, thereby synergistically reducing efficiency. mouse piglet systemic models, showed favorable therapeutic efficacy, significantly load levels inflammation factors. Overall, this study provides perspectives developing biomimetic stimuli‐responsive combat infections.

Language: Английский

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Novel and diverse features identified in the genomes of bacteria isolated from a hydrothermal vent plume

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

ABSTRACT Hydrothermal vent plumes (HVPs), formed by high-temperature emissions, are rich in compounds that support chemosynthesis and serve as reservoirs of microbial diversity genetic innovation. Through turbulence, mixing, interaction with subsea currents, communities thought to disperse across ocean basins. In this study, we focused on the plume Moytirra hydrothermal field, a relatively unexplored site, investigate its inhabitants. We cultured bacteria from HVP using 11 different media types performed complete genome sequencing 12 isolates. Our analyses revealed four putatively novel species Thalassobaculum , Sulfitobacter Idiomarina Christiangramia genera. Comparative genomics identified unique genomic islands containing biosynthetic gene clusters, including Non-Ribosomal Peptide Synthetase/Polyketide Synthase cluster, toxin-antitoxin systems, evidence horizontal transfer facilitated prophages. These findings underscore potential HVPs source biotechnologically relevant genes, contributing our understanding biodiversity complexity these extreme environments. IMPORTANCE vents dynamic environments offer nutrients for chemosynthetic organisms drive biology deep-sea. The dynamics ecosystems innovation resident populations. (HVPs) mix surrounding water, carrying local microbiota them dispersing hundreds kilometers. This study isolated capture snapshot community, revealing within three taxonomic classes. addition genomes public databases provides valuable insights into function, architecture, clusters found

Language: Английский

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Antibacterial effects of cinnamaldehyde and hesperitin on resistant Glaesserella parasuis by suppressing QseBC two-component system

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: April 12, 2025

Language: Английский

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ΔFleQ of Aeromonas hydrophila generated as a live attenuated vaccine in common carp (Cyprinus carpio)

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110361 - 110361

Published: April 1, 2025

Language: Английский

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