The Long-Noncoding RNA TUG1 Regulates Oxygen-Induced Retinal Neovascularization in Mice via MiR-299 DOI Creative Commons
Yue Wang, Xue Wang, Yuexia Wang

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2022, Volume and Issue: 63(1), P. 37 - 37

Published: Jan. 27, 2022

Purpose: The oxygen-induced retinal neovascularization mouse model closely approximates pathological changes associated with human neovascularization-associated diseases, including retinopathies. We used this and endothelial cells (HRECs) under hypoxia to explore the relationship between taurine upregulated gene-1 (TUG1), vascular growth factor (VEGF), miR-299-3p on retinopathy of prematurity (ROP). Methods: An (OIR) was established; mice were divided into a normal control group, OIR TUG1 group (lentivirus control), TUG1-knockdown group. apoptosis evaluated using TUNEL assay. Angiogenic, apoptotic, inflammatory factors detected by Western blot, immunohistochemistry, immunofluorescence analyses. HRECs cultured assessed for VEGF expression, apoptosis, tubule formation, migration ability. TUG1, VEGF, via dual luciferase reporter gene Results: Intravitreal injection lentivirus reduced response in tissue markedly retina. Overexpression miR-299 rate, tube ability hypoxia-treated cells, thereby inhibiting formation new blood vessels. assay suggested that has binding sites VEGF. Conclusions: reduces expression VEGFA competitively adsorbing facilitates regulation neovascularization, suggesting it may serve as therapeutic target neovascular diseases.

Language: Английский

N6-Methyladenosine-modified circSAV1 triggers ferroptosis in COPD through recruiting YTHDF1 to facilitate the translation of IREB2 DOI
Haibo Xia,

Yan Wu,

Jing Zhao

et al.

Cell Death and Differentiation, Journal Year: 2023, Volume and Issue: 30(5), P. 1293 - 1304

Published: Feb. 24, 2023

Language: Английский

Citations

79
Understanding exosomes: Part 2—Emerging leaders in regenerative medicine DOI Creative Commons
Richard J. Miron, Nathan E. Estrin, Anton Sculean

et al.

Periodontology 2000, Journal Year: 2024, Volume and Issue: 94(1), P. 257 - 414

Published: Feb. 1, 2024

Abstract Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with ability to communicate other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due their be utilized as therapeutic options for a wide array diseases/conditions. Over 5000 publications currently being published yearly on this topic, number is only expected dramatically increase novel strategies continue developed. Today exosomes have been applied numerous contexts including neurodegenerative disorders (Alzheimer's disease, central nervous system, depression, multiple sclerosis, Parkinson's post‐traumatic stress disorders, traumatic brain injury, peripheral nerve injury), damaged organs (heart, kidney, liver, stroke, myocardial infarctions, ovaries), degenerative processes (atherosclerosis, diabetes, hematology musculoskeletal degeneration, osteoradionecrosis, respiratory disease), infectious diseases (COVID‐19, hepatitis), procedures (antiaging, bone regeneration, cartilage/joint osteoarthritis, cutaneous wounds, dental dermatology/skin erectile dysfunction, hair regrowth, intervertebral disc repair, spinal cord vascular regeneration), cancer therapy (breast, colorectal, gastric osteosarcomas), immune function (allergy, autoimmune regulation, inflammatory diseases, lupus, rheumatoid arthritis). This scoping review first its kind aimed at summarizing extensive potential broad range disorders.

Language: Английский

Citations

19
APAF1‐Binding Long Noncoding RNA Promotes Tumor Growth and Multidrug Resistance in Gastric Cancer by Blocking Apoptosome Assembly DOI
Qiang Wang, Chen Chen, Xiao Xu

et al.

Advanced Science, Journal Year: 2022, Volume and Issue: 9(28)

Published: Aug. 17, 2022

Abstract Chemotherapeutics remain the first choice for advanced gastric cancers (GCs). However, drug resistance and unavoidable severe toxicity lead to chemotherapy failure poor prognosis. Long noncoding RNAs (lncRNAs) play critical roles in tumor progression many cancers, including GC. Here, through RNA screening, an apoptotic protease‐activating factor 1 (APAF1)‐binding lncRNA (ABL) that is significantly elevated cancerous GC tissues independent prognostic patients identified. Moreover, ABL overexpression inhibits cell apoptosis promotes survival multidrug xenograft organoid models. Mechanistically, directly binds RNA‐binding protein IGF2BP1 via its KH1/2 domain, then further recognizes METTL3‐mediated m6A modification on ABL, which maintains stability. In addition, can bind WD1/WD2 domain of APAF1, competitively prevent cytochrome c from interacting with blocking apoptosome assembly caspase‐9/3 activation; these events death cells. Intriguingly, targeting using encapsulated liposomal siRNA enhance sensitivity cells chemotherapy. Collectively, results suggest be a potential biomarker therapeutic target

Language: Английский

Citations

52
Leucine improves the aerosol performance of dry powder inhaler formulations of siRNA-loaded nanoparticles DOI Creative Commons
You Xu,

Laure Harinck,

Abhijeet Lokras

et al.

International Journal of Pharmaceutics, Journal Year: 2022, Volume and Issue: 621, P. 121758 - 121758

Published: April 25, 2022

Thermostable dry powder inhaler (DPI) formulations with high aerosol performance are attractive inhalable solid dosage forms for local treatment of inflammatory lung diseases. We recently demonstrated that lipidoid-polymer hybrid nanoparticles (LPNs) loaded small interfering RNA (siRNA) directed against tumor necrosis factor alpha (TNF-α) mediate efficient intracellular siRNA delivery and reduce inflammation in vivo. Here, we show mixtures the stabilizing excipients trehalose (Tre) dextran (Dex), combination shell-forming dispersion enhancer leucine (Leu), stabilize TNF-α siRNA-loaded LPNs during spray drying into nanocomposite microparticles, result DPI performance. At low Leu content (0 to 10%, w/w), were amorphous, exhibited poor When was increased from 20 60% (w/w), surface 39.2 68.1 mol%, flowability significantly improved. Microscopy analysis suggest improved dispersibility is a wrinkled morphology, which reduces area available interparticle interactions. Increasing further (to above w/w) did not influence performance, yield maximal at 30-40% (w/w). Formulations containing 40% Tre:Dex ratio 10:90 (w/w) displayed fine particle fraction properties suitable inhalation. The chemical integrity preserved state, biodistribution studies mice showed pulmonary administration resulted homogenous deep deposition. Our results demonstrate optimal ratios, ternary excipient Leu, Tre Dex protect drying. Hence, this study shows microparticles an amorphous Tre/Dex matrix crystalline shell efficiently ensure

Language: Английский

Citations

43
Applications and advancements of nanoparticle-based drug delivery in alleviating lung cancer and chronic obstructive pulmonary disease DOI
Gabriele De Rubis, Keshav Raj Paudel, Leander Corrie

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2023, Volume and Issue: 397(5), P. 2793 - 2833

Published: Nov. 22, 2023

Language: Английский

Citations

33
mRNA Technology in Modern Medicine: Review and Future Prospects DOI Creative Commons
Heslley Machado Silva

Deleted Journal, Journal Year: 2025, Volume and Issue: 2, P. 1 - 1

Published: Jan. 17, 2025

Messenger RNA (mRNA) technology has revolutionized modern medicine, particularly in developing vaccines and gene therapies. While its prominence soared during the COVID-19 pandemic, foundation was built on decades of meticulous research. This review explores historical evolution mRNA technology, stabilization delivery breakthroughs, applications combating infectious diseases, cancer, genetic disorders. The study utilized a systematic search peer-reviewed articles from leading databases such as PubMed Scopus, focusing advancements clinical applications. Future potential treating chronic enhancing immunotherapy, addressing public health emergencies is also discussed, emphasizing need for sustained research innovation to harness transformative capabilities fully.

Language: Английский

Citations

1
Pulmonary siRNA delivery for lung disease: Review of recent progress and challenges DOI
Ling Ding, Siyuan Tang, Todd A. Wyatt

et al.

Journal of Controlled Release, Journal Year: 2020, Volume and Issue: 330, P. 977 - 991

Published: Nov. 9, 2020

Language: Английский

Citations

60
Aerosol pulmonary immune engineering DOI Creative Commons
Emma R. Sudduth, Michael Trautmann-Rodriguez,

Nicole Gill

et al.

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 199, P. 114831 - 114831

Published: April 24, 2023

Aerosolization of immunotherapies poses incredible potential for manipulating the local mucosal-specific microenvironment, engaging specialized pulmonary cellular defenders, and accessing mucosal associated lymphoid tissue to redirect systemic adaptive memory responses. In this review, we breakdown key inhalable immunoengineering strategies chronic, genetic, infection-based inflammatory disorders, encompassing historic use immunomodulatory agents, transition biological inspired or derived treatments, novel approaches complexing these materials into drug delivery vehicles enhanced release outcomes. Alongside a brief description immune targets, fundamentals aerosol delivery, preclinical models response, survey recent advances inhaled immunotherapy platforms, ranging from small molecules biologics particulates cell therapies, as well prophylactic vaccines. each section, address formulation design constraints advantages platform in driving desirable modifications. Finally, prospects clinical translation outlook engineering are discussed.

Language: Английский

Citations

19
The Potential Roles of Exosomes in Chronic Obstructive Pulmonary Disease DOI Creative Commons
Nan Wang, Qin Wang, Tiantian Du

et al.

Frontiers in Medicine, Journal Year: 2021, Volume and Issue: 7

Published: Jan. 14, 2021

Currently, chronic obstructive pulmonary disease (COPD) is one of the most common lung diseases. Chronic characterized by progressive loss function due to inflammatory responses in lungs caused repeated exposure harmful environmental stimuli. a persistent disease, with an estimated 384 million people worldwide living COPD. It listed as third leading cause death. Exosomes contain various components, such lipids, microRNAs (miRNAs), long non-coding RNAs(lncRNAs), and proteins. They are essential mediators intercellular communication can regulate biological properties target cells. With deepening exosome research, it found that exosomes strictly related occurrence development Therefore, this review aims highlight unique role immune-cell-derived through complex interactions their potentials potential biomarkers new types

Language: Английский

Citations

36
Advanced human-relevant in vitro pulmonary platforms for respiratory therapeutics DOI
Arbel Artzy‐Schnirman,

Sivan Arber Raviv,

Ofri Doppelt Flikshtain

et al.

Advanced Drug Delivery Reviews, Journal Year: 2021, Volume and Issue: 176, P. 113901 - 113901

Published: July 29, 2021

Language: Английский

Citations

33