The uses of zebrafish (Danio rerio) as an in vivo model for toxicological studies: A review based on bibliometrics

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 272, P. 116023 - 116023

Published: Jan. 30, 2024

An in vivo model is necessary for toxicology. This review analyzed the uses of zebrafish (Danio rerio) toxicology based on bibliometrics. Totally 56,816 publications about from 2002 to 2023 were found Web Science Core Collection, with Toxicology as top 6 among all disciplines. Accordingly, bibliometric map reveals that "toxicity" has become a hot keyword. It further most common exposure types include acute, chronic, and combined exposure. The toxicological effects behavioral, intestinal, cardiovascular, hepatic, endocrine toxicity, neurotoxicity, immunotoxicity, genotoxicity, reproductive transgenerational toxicity. mechanisms oxidative stress, inflammation, autophagy, dysbiosis gut microbiota. toxicants commonly evaluated by using nanomaterials, arsenic, metals, bisphenol, dioxin. Overall, provide unique well-accepted investigate mechanisms. We also discussed possible ways address some limitations model, such combination human organoids avoid species differences.

Language: Английский

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Oral exposure to nanoplastics altered lipid profiles in mouse intestine

Food and Chemical Toxicology, Journal Year: 2025, Volume and Issue: 197, P. 115304 - 115304

Published: Feb. 2, 2025

Language: Английский

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The uses of transcriptomics and lipidomics indicated that direct contact with graphene oxide altered lipid homeostasis through ER stress in 3D human brain organoids

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 849, P. 157815 - 157815

Published: Aug. 3, 2022

Language: Английский

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The Development of Yellow Mealworm (Tenebrio molitor) as a Cheap and Simple Model to Evaluate Acute Toxicity, Locomotor Activity Changes, and Metabolite Profile Alterations Induced by Nanoplastics of Different Sizes

Journal of Applied Toxicology, Journal Year: 2025, Volume and Issue: 45(6), P. 994 - 1003

Published: Feb. 9, 2025

Due to the wide uses of plastic products, nanoplastics are ubiquitous contaminants in environment. Hence, extensive studies used various models evaluate toxicity nanoplastics. In present study, we developed yellow mealworm (Tenebrio molitor) as an alternative model investigate acute Our results showed that microinjection with 500 mg/kg significantly increased death rate mealworms after 24 or 48 h, 100 nm particles being more effective compared 20 ones. Meanwhile, dose-dependent increase was observed injection 2-200 Exposure 2 but not also led hyperactivity mealworms. Both types altered metabolite profiles, up-regulated and down-regulated 9 12 metabolites, whereas 16 25 respectively. Enrichment analysis revealed affected alpha-linolenic acid metabolism (ko00592) purine (ko00230). For metabolites belonging these pathways, stearidonic guanine. Combined, size-dependent effects on toxicity, profile changes These indicated potential a cheap simple

Language: Английский

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Intratracheal instillation of graphene oxide with different diameters suppressed toll-like receptor 3-mediated lipid droplet biogenesis in lungs and livers of mice

Toxicology Research, Journal Year: 2025, Volume and Issue: 14(3)

Published: May 2, 2025

Abstract Recent advances have established lipid droplets as dynamic innate immune hubs coordinating cellular metabolism and defense mechanisms. While previous studies primarily focused on nanomaterials (NMs) altering to influence droplet dynamics, this study pioneers the investigation of NM-induced modulation via Toll-like receptor (TLR) pathways a novel regulatory axis for droplets. Building our prior findings that graphene oxide (GO) impaired TLR3-mediated signaling, we systematically explored role GO’s diameter in modulating process. Mice were subjected daily intratracheal instillation three GO variants (50–200 nm, <500 nm or > 500 nm) at 1 mg/kg 7 days. Although no significant change body weight organ coefficient was observed, all exposure suppressed staining mouse lungs livers, correlating with altered co-localization TLR3 perilipin 2 (PLIN2), critical regulators biogenesis. Down-regulation signaling components, namely interferon induced protein tetratricopeptide repeats (IFIT1), radical S-adenosyl methionine domain containing (RSAD2), PLIN2, occurred diameter-dependent manner, 50–200 showing most pronounced effects, likely attributable smallest hydrodynamic size polydispersity index suspension. This work provides evidence NM geometry governs TLR-mediated regulation, bridging knowledge gap between nanotoxicology immunometabolic cross-talking, paradigm distinct from conventional metabolism-focused nanotoxicological studies.

Language: Английский

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Comparison of developmental toxicity of graphene oxide and graphdiyne to zebrafish larvae

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology, Journal Year: 2024, Volume and Issue: 281, P. 109924 - 109924

Published: April 13, 2024

Language: Английский

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An update on the biological effects of quantum dots: From environmental fate to risk assessment based on multiple biological models

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 879, P. 163166 - 163166

Published: April 1, 2023

Language: Английский

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Exposure to MoS2 nanosheets or bulk activated Kruppel‐like factor 4 in 3D Caco‐2 spheroids in vitro and mouse intestines in vivo

Environmental Toxicology, Journal Year: 2023, Volume and Issue: 38(8), P. 1925 - 1938

Published: April 26, 2023

MoS2 nanosheets (NSs) are novel 2D nanomaterials (NMs) being used in many important fields. Recently, we proposed the need to evaluate influences of NMs on Kruppel-like factors (KLFs) even if these materials relatively biocompatible. In this study, investigated NSs or bulk KLF4 signaling pathway 3D Caco-2 spheroids vitro and mouse intestines vivo. Through analysis our previous RNA-sequencing data, found that exposure activated expression spheroids. Consistently, also KLF4-related gene ontology (GO) terms down-regulated a panel KLF4-downstream genes. To verify findings, repeatedly exposed mice via intragastrical administration (1 mg/kg bodyweight, once day, for 4 days). It was shown oral decreased leading higher organ coefficients. As expected, both types increased Mo elements as well other trace elements, such Zn, Fe, Mn intestines. The induced morphological changes intestines, shortening intestinal villi crypt depth, which may result lipid staining. Consistent with material protein staining two downstream proteins, namely extracellular signal-regulated kinase (ERK) serine/threonine (AKT). We concluded were capable activate KLF4-signaling vivo vitro.

Language: Английский

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Effects of multi‐walled carbon nanotubes and halloysite nanotubes on plasma lipid profiles and autophagic lipolysis pathways in mouse aortas and hearts

Environmental Toxicology, Journal Year: 2024, Volume and Issue: 39(9), P. 4431 - 4446

Published: June 10, 2024

Abstract Multi‐walled carbon nanotubes (MWCNTs) and halloysite (HNTs) are widely used tubular‐structured nanomaterials (NMs), but their cardiovascular effects not clear. This study compared the of MWCNTs HNTs on lipid profiles in mouse plasma gene expression aortas hearts. Mice were intravenously injected with 50 μg NMs, once a day, for 5 days. Then, was collected lipidomics analysis, hearts RNA‐sequencing analysis. While or did induce obvious pathological changes hearts, altered. Further analysis revealed that more effectively upregulated sphingolipids sterol lipids, whereas glycerophospholipids fatty acyls. Consistently, data indicated altered signaling pathways related synthesis metabolism, as well those endoplasmic reticulum, lysosomes autophagy, significantly than We further verified proteins involved autophagic lipolysis, to suppress biomarker LC3, affect metabolism proteins. These results may provide novel understanding about influences systems. Importantly, previous studies considered biocompatible materials, from this suggested both capable lipolysis systems, although exact different.

Language: Английский

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Influences of TiO₂ nanoparticle and fipronil co‐exposure on metabolite profiles in mouse intestines

Journal of Applied Toxicology, Journal Year: 2024, Volume and Issue: 44(11), P. 1793 - 1803

Published: July 29, 2024

Abstract Food contaminates, such as insecticide, may influence the toxicity of nanoparticles (NPs) to intestine. The present study investigated combined TiO 2 NPs and fipronil male mouse Juvenile mice (8 weeks) were orally exposed 5.74 mg/kg NPs, 2.5 fipronil, or both, once a day, for 5 days. We found that both induced some pathological changes in intestines, accompanying with defective autophagy, but these effects not obviously enhanced after NP co‐exposure. Fipronil promoted Ti accumulation minimal impact on other trace elements NP‐exposed intestines. Metabolomics data revealed exposure altered metabolite profiles two KEGG pathways, namely, ascorbate aldarate metabolism (mmu00053) glutathione (mmu00480), only statistically significantly changed Five metabolites, including 2‐deoxy‐D‐erythro‐pentofuranose 5‐phosphate, 5alpha‐cholestanol, beta‐D‐glucopyranuronic acid, elaidic isopentadecanoic maltotriose, more up‐regulated co‐exposure, whereas trisaccharide xylonolactone down‐regulated by concluded had enhance intestines profiles.

Language: Английский

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