Regenerative mesenchymal stem cell‐derived extracellular vesicles: A potential alternative to cell‐based therapy in viral infection and disease damage control DOI Creative Commons
Ayodeji O. Ipinmoroti, Rachana Pandit, Qiana L. Matthews

et al.

WIREs Mechanisms of Disease, Journal Year: 2022, Volume and Issue: 14(6)

Published: Sept. 5, 2022

Abstract Extracellular vesicles (EVs) released by regenerative cells such as mesenchymal stem are effective facilitators of healing, therapy, and repair. Conversely, EVs from infected and/or diseased could be useful markers in the detection diagnosis disease conditions cancer at their earliest most detectable, treatable stage. A very important type EVs, termed exosomes offer a hypothetical new paradigm detection, diagnosis, treatment. broad range exosome‐based biomedical therapeutic applications now being evaluated recent clinical trials. Exosomes found virtually all bodily fluids capable crossing tight junctions toughly regulated boundaries blood–brain barrier. Exosomes' expedition ends when they taken up bystander which corroborates fact that conduits for releasing them. have been associated with cell‐to‐cell progression diseases like viral disease, neurodegeneration, certain cancers. Due to high discrimination conditions, exosome uptake is usually cell‐specific. Lots research evidence revealed infusion derived impede development infections age‐related activating self‐repair machinery through RNA, DNA, protein, lipid transfer between patients. They also demonstrated restoration circulating population tissues fluid recipients. The first human trials therapies underway, establishing future medicine. This article categorized under: Cancer > Stem Cells Development Immune System Diseases Molecular Cellular Physiology

Language: Английский

Artificial intelligence and guidance of medicine in the bubble DOI Creative Commons
Asma Akbar,

Nagavalli Pillalamarri,

Sriya Jonnakuti

et al.

Cell & Bioscience, Journal Year: 2021, Volume and Issue: 11(1)

Published: June 9, 2021

Abstract Microbubbles are nanosized gas-filled bubbles. They used in clinical diagnostics, medical imaging, as contrast agents ultrasound and transporters for targeted drug delivery. can also be to treat thrombosis, neoplastic diseases, open arteries vascular plaques localized transport of chemotherapies cancer patients. filled with any type therapeutics, cure agents, growth factors, extracellular vesicles, exosomes, miRNAs, drugs. protect their cargo from immune attack because specialized encapsulated shell composed lipid protein. Filled curative medicine, they could effectively circulate through the whole body safely efficiently reach target area. The advanced bubble-based drug-delivery system, integrated artificial intelligence guidance, holds great promise delivery drugs medicines.

Language: Английский

Citations

17
Thyroid Cancer and COVID-19: Prospects for Therapeutic Approaches and Drug Development DOI Creative Commons
Na Qu,

Zongguang Hui,

Zhixin Shen

et al.

Frontiers in Endocrinology, Journal Year: 2022, Volume and Issue: 13

Published: May 4, 2022

Thyroid cancer is the most prevalent endocrine malignancy and reported incidence of thyroid has continued to increase in recent years. Since 2019, coronavirus disease 2019 (COVID-19) been spreading worldwide a global pandemic. COVID-19 aggravates primary illnesses affects management; relevant changes include delayed diagnosis treatment. The an organ that susceptible autoimmune attack; thus, after gradually attracted attention. Whether treatment also attention many researchers. This review examines literature regarding influence on pathogenesis, diagnosis, cancer; it focuses drug therapies promote research into strategies for improving therapy management patients with COVID-19.

Language: Английский

Citations

12
Extracellular vesicles in vaccine development and therapeutic approaches for viral diseases DOI Creative Commons
Parisa Moradi Pordanjani, Azam Bolhassani, Alireza Milani

et al.

Process Biochemistry, Journal Year: 2023, Volume and Issue: 128, P. 167 - 180

Published: Feb. 28, 2023

Language: Английский

Citations

7
The Role of Extracellular Vesicles in COVID-19 Pathology DOI Creative Commons
Aline Seiko Carvalho Tahyra, Rodrigo T. Calado, Fausto Almeida

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(16), P. 2496 - 2496

Published: Aug. 11, 2022

Extracellular vesicles (EVs) have become a trending topic in recent years; they constitute new intercellular communication paradigm. are 30-4000 nanometers diameter particles that limited by phospholipid bilayer and contain functional biomolecules, such as proteins, lipids, nucleic acids. They released virtually all types of eukaryotic cells; through their cargoes, EVs capable triggering signaling recipient cells. In addition to functions the homeostatic state, gained attention because roles pathological contexts, eventually contributing disease progression. Coronavirus 2019 (COVID-19) pandemic, aside from scientific race for development preventive therapeutic interventions, it is critical understand mechanisms involved SARS-CoV-2 infection. this sense, key players main processes COVID-19. Thus, review, we highlight role establishment viral infection procoagulant cytokine storm, immunoregulation innate adaptive immune responses.

Language: Английский

Citations

11
Bioengineered extracellular vesicles: future of precision medicine for sepsis DOI Creative Commons
Aina Areny‐Balagueró, Anna Solé‐Porta, Marta Camprubí‐Rimblas

et al.

Intensive Care Medicine Experimental, Journal Year: 2023, Volume and Issue: 11(1)

Published: March 10, 2023

Abstract Sepsis is a syndromic response to infection and frequently final common pathway death from many infectious diseases worldwide. The complexity high heterogeneity of sepsis hinder the possibility treat all patients with same protocol, requiring personalized management. versatility extracellular vesicles (EVs) their contribution progression bring along promises for one-to-one tailoring treatment diagnosis. In this article, we critically review endogenous role EVs in how current advancements have improved EVs-based therapies toward translational future clinical application, innovative strategies enhance effect. More complex approaches, including hybrid fully synthetic nanocarriers that mimic EVs, are also discussed. Several pre-clinical studies examined through offer general outlook perspectives EV-based diagnosis treatment.

Language: Английский

Citations

5
Heat shock protein 20 promotes sirtuin 1-dependent cell proliferation in induced pluripotent stem cells DOI Creative Commons
Mujib Ullah, Nicole Min Qian Pek, Gustavo Yannarelli

et al.

World Journal of Stem Cells, Journal Year: 2021, Volume and Issue: 13(6), P. 659 - 669

Published: June 24, 2021

BACKGROUNDHeat shock proteins (HSPs) are molecular chaperones that protect cells against cellular stresses or injury.However, it has been increasingly recognized they also play crucial roles in regulating fundamental processes.HSP20 implicated cell proliferation, but conflicting studies have shown can either promote suppress proliferation.The underlying mechanisms by which HSP20 regulates proliferation and pluripotency remain unexplored.While the effect of on recognized, its role inducing human-induced pluripotent stem (iPSCs) not addressed. AIMTo evaluate efficacy overexpression human iPSCs ability to purpose this study was investigate whether increase regeneration. METHODSWe used iPSCs, retain their potential for proliferation.HSP20 effectively enhanced pluripotency.Overexpression

Language: Английский

Citations

11
Characterization of extracellular vesicles in COVID-19 infection during pregnancy DOI Creative Commons

Ayelet Dangot,

Mor Zavaro,

Tali Hana Bar-Lev

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: July 25, 2023

Introduction: SARS-CoV-2 infection may cause a severe inflammatory response, inflicting morbidity and mortality. This risk is modestly increased in pregnant patients. Despite the hypercoagulability immunosuppression associated with pregnancy, most women experience mild COVID-19 infection. Maternal extracellular vesicles (EVs) interact endothelial immune components to facilitate favorable disease course. pilot study aimed explore characteristics of EVs released during occurring third trimester pregnancy. Methods: In this prospective study, blood samples were obtained from 16 healthy non-pregnant (NP), 18 healthy-pregnant (HP), 22 positive subjects (CoV-P). Disease course pregnancy outcomes assessed characterized. Of note, limited volumes sample acquired made it necessary use smaller different subsets for each analysis. Results: The majority (91%) COVID-19-pregnant (18 2 moderate disease) experienced good pregnancy-related outcomes. EV concentrations higher compared (p = 0.0041) lower 0.0150). CD63 exosome marker expression was 0.0149, p 0.0028, respectively). Similar levels entry proteins (ACE-2 TMPRSS2) found all three groups. Cytokine content subject-EVs EVs, while IL-2 IL-6 decreased 0.043, 0.0390, CD8+, cytotoxic T-cell marker, 0.0108, < 0.0001, COVID-19- demonstrated platelet activation (CD62P) than 0.0327) 0.0365). Endothelial EV-CD144+ versus 0.0093), but similar subjects. Other EVs' coagulation markers/activity, D-Dimer fibrinogen Conclusion: subjects' an attenuated no additional system.

Language: Английский

Citations

3
SAR-CoV-2 infection, emerging new variants and the role of activation induced cytidine deaminase (AID) in lasting immunity DOI Creative Commons
Asad Ullah,

Neelam Mabood,

Muhammad Maqbool

et al.

Saudi Pharmaceutical Journal, Journal Year: 2021, Volume and Issue: 29(10), P. 1181 - 1184

Published: Sept. 21, 2021

As the world faces a fourth COVID-19 spike, scientists are learning lot more about new SARS-CoV-2 strains that were previously unknown. Currently, Delta versions of have become prevalent in much since it first appeared India late 2020. Researchers believe they discovered why has been so successful: those infected with create significantly virus than original strain SARS-CoV-2, making extremely contagious. This redirected focus to how our immune system defends us from these various pathogens and initiates such varied responses. Hundreds research papers published on origins long-lasting responses disparities numbers different cell types COVID 19 survivors, but primary architect discrepancies yet be discovered. In this essay, we will concentrate protein, activation induced cytidine deaminase (AID), which triggers molecular processes allow produce powerful antibodies specific B cells, allowing outwit virus. We if ever achieve permanent immunity infection, AID key releasing it.

Language: Английский

Citations

7
Cytidine deamination-induced perpetual immunity to SAR-CoV-2 infection is a potential new therapeutic target DOI Creative Commons
Asad Ullah,

Neelam Mabood,

Muhammad Maqbool

et al.

International Journal of Medical Sciences, Journal Year: 2021, Volume and Issue: 18(16), P. 3788 - 3793

Published: Jan. 1, 2021

As the world is racing to develop perpetual immunity SARS-CoV-2 virus. The emergence of new viral strains, together with vaccination and reinfections, are all contributing a long-term against deadly virus that has taken over since its introduction humans in late December 2019. discovery more than 95 percent people who recovered from COVID-19 had long-lasting asymptomatic have different immune response symptomatic shifted attention how our system initiates such diverse responses. These findings provided reason believe days numbered. Hundreds research papers been published on causes responses variations numbers cell types COVID 19 survivors, but main these differences still not adequately identified. In this article, we focus activation-induced cytidine deaminase (AID), which molecular processes allow generate antibodies SARS-CoV-2. To establish lasting SARS-CoV-2, suggest AID could be key unlocking it.

Language: Английский

Citations

7
Enhancement of membrane vesicle production by disrupting the degP gene in Meiothermus ruber H328 DOI Creative Commons

Yuki Asano,

Manato Onishi,

Kaito Nishi

et al.

AMB Express, Journal Year: 2021, Volume and Issue: 11(1)

Published: Dec. 1, 2021

The phenomenon of membrane vesicle (MV) production is known to be common all bacterial cells. Although MVs are expected employed in a variety applications, improving MV productivity essential for applications. Since the deletion degP gene, periplasmic dual-function protease and chaperone, Escherichia coli has successfully improved capacity, we tried enhance thermophilic M. ruber H328 by deleting gene. One gene (mrH_0331) was selected from genome constructed mutant strain ∆degP that replaced with htk showing kanamaycin resistance homologous recombination. exhibited smooth growth but lower level turbidity at 60 °C although there no difference 55 between wild strain. Finally, have confirmed incubation increases about fivefold using two fluorescent dyes, DiI FM4-64, which followed TEM analysis. presumably causes an increase denatured proteins °C, leading enhanced production. Meanwhile, S-layer protein included outer increased fraction prepared cells incubated °C. This indicates this method effective enhances it H328.

Language: Английский

Citations

5