Microvascular Research, Journal Year: 2021, Volume and Issue: 136, P. 104172 - 104172
Published: April 21, 2021
Language: Английский
Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2020, Volume and Issue: 19(6), P. 3130 - 3158
Published: Sept. 4, 2020
Abstract Chlorogenic acids (CGAs), a group of hydroxycinnamates, are generally abundant in everyday foods and beverages, most prominently certain coffee drinks. Among them, the chlorogenic acid (CGA), also termed as 5‐ O ‐caffeoylquinic (5‐CQA), is one abundant, highly functional polyphenolic compounds human diet. The evidence its health benefits obtained from clinical studies, well basic research, indicates an inverse correlation between 5‐CQA consumption lower risk metabolic syndromes chronic diseases. This review focuses on beneficial properties for mechanisms action 5‐CQA, starting with history, isomers, dietary sources, processing effects, preparation methods, pharmacological safety evaluation, bioavailability. It provides possible molecular mechanistic bases to explain effects including neuroprotective, cardiovascular protective, gastrointestinal renoprotective, hepatoprotective, glucose lipid metabolism regulatory, anticarcinogenic effects. information summarized here could aid research natural additive, potential drug candidate, promoter.
Language: Английский
Citations
313
Molecular Medicine Reports, Journal Year: 2021, Volume and Issue: 23(5)
Published: March 1, 2021
Atherosclerosis (AS) is a chronic inflammatory disease of the vascular wall with multiple causes. AS primary pathological basis cardiovascular and stroke. Moreover, carotid plaque rupture thrombus formation are main causes ischemic Therefore, understanding plaques may help improve prediction prevention cerebrovascular events. Endothelial cell dysfunction results in re‑endothelialization angiogenesis atherosclerotic plaques, thus promoting destabilization. The aim present study was to evaluate effect circular RNA (circRNA) molecules serum exosomes (serum‑Exos) from patients stable atherosclerosis (SA) unstable/vulnerable (UA). Specifically, circRNA on human umbilical vein endothelial (HUVEC) behavior mechanisms underlying destabilization were evaluated. Serum‑Exos isolated, then identified using transmission electron microscopy, nanoparticle tracking analysis western blotting. serum‑Exo‑circRNA expression profile SA or UA investigated array. relationship between circRNA‑006896 serum‑Exos biochemical parameters analyzed Spearman's correlation. In addition, HUVECs incubated for in vitro functional assays. demonstrated that circRNAs profiles significantly different, indicating potential role circRNA‑0006896 positively correlated triglyceride, low‑density lipoprotein cholesterol (LDL‑C) C‑reactive protein levels, negatively albumin levels UA. However, LDL‑C SA. Using bioinformatic analysis, competing endogenous (ceRNA) network selected regulatory roles serum‑Exos. Additionally, treated derived UA, upregulated. This accompanied by decreased microRNA‑1264 SOCS3, increased DNMT1 phosphorylated STAT3. HUVEC proliferation migration group, compared mock groups. finding indicates circRNA‑0006896‑miR-1264‑DNMT1 axis plays an important regulating cells. it suggests represent therapeutic target controlling JNK/STAT3 signaling HUVECs. Thus, this provide insight interventions against vulnerable AS.
Language: Английский
Citations
56
Cancer Gene Therapy, Journal Year: 2022, Volume and Issue: 29(12), P. 1988 - 2000
Published: Aug. 1, 2022
Abstract Although androgen receptor (AR) can influence bladder cancer (BCa) initiation and progression, its impact on tumor immune escape remains unclear. Here, we found that targeting AR could enhance natural killer (NK) cell tumor-killing efficacy by decreasing PD-L1 expression. Both antiandrogen treatment knockdown effectively reduced membrane PD-LI expression to facilitate NK cell-mediated BCa killing downregulating circ_0001005. Mechanistically, upregulated circRNA circ_0001005 via the RNA-editing gene ADAR2. competitively sponged miRNA miR-200a-3p promote A preclinical xenograft mouse model further confirmed this newly identified signaling using small molecule circ_0001005-shRNA improve of cells. Collectively, these results suggest ADAR2/circ_0001005/miR-200a-3p/PD-L1 pathway antitumor immunity may suppress progression boost immunotherapeutic in BCa.
Language: Английский
Citations
35
Heliyon, Journal Year: 2024, Volume and Issue: 10(4), P. e25515 - e25515
Published: Feb. 1, 2024
Facing the growing issue of cardiovascular diseases, metallic materials with higher tensile strength and fatigue resistance play an important role in treating diseases. This review lists advantages drawbacks commonly used medical for vascular stents. To avoid post-procedural threats such as thrombosis in-stent restenosis, surface treatments, coating methods have been to further improve biocompatibility these materials. Surface treatments including laser, plasma treatment, polishing, oxidization, fluorination can by modifying charges, morphology, properties material. Coating based on polymer coatings, carbon-based drug-functional coatings regulate properties, also serve effective barrier interaction biomaterial surfaces biomolecules, which be corrosion stability, well their biocompatibility. Biocompatibility serves most fundamental property stents, maintaining excellent stable stent is a current research bottleneck. Few reviews published biomaterials stents treatments. For purpose advancing common metal biomaterials, treatment methods, comprehensive are described this review. Finally, we suggest future directions development, continuously improving durability stability permanent accelerating development biodegradable strengthening feedback safety reliability
Language: Английский
Citations
8
Metabolites, Journal Year: 2020, Volume and Issue: 10(10), P. 391 - 391
Published: Sept. 30, 2020
Liraglutide has shown favourable effects on several cardiometabolic risk factors, beyond glucose control. MicroRNAs (miRNAs) regulate gene expression, resulting in post-transcriptional modifications of cell response and function. Specific miRNAs, including miRNA-27b, miRNA-130a, miRNA-210, play a role disease. We aimed to determine the effect liraglutide serum levels miRNA-130a miRNA-210. Twenty-five subjects with type-2 diabetes mellitus (T2DM), naïve incretin-based therapy, were treated (1.2 mg/day as an add-on metformin) for 4 months. miRNAs quantified using real-time polymerase chain reaction. After treatment, we found significant reductions fasting (from 9.8 ± 5.3 6.7 1.6 mmol/L, p = 0.0042), glycosylated haemoglobin (HbA1c) 8.1 0.8 6.6 1.0%, 0.0008), total cholesterol 5.0 1.0 4.0 0.7 0.0011), triglycerides 1.9 1.5 0.0104) low-density lipoprotein 2.9 1.2 2.2 0.6 0.0125), while miRNA-210a significantly increased (median (interquartile range, IQR) changes: 1.73 (7.12) (p 0.0401), 1.91 (3.64) 0.0401) 2.09 (11.0) 0.0486), respectively). Since changes independent all metabolic parameters investigated, seems exert direct epigenetic T2DM patients, regulating microRNAs involved maintenance endothelial homeostasis. These might be implicated liraglutide's benefits may represent useful targets management.
Language: Английский
Citations
46
FEBS Journal, Journal Year: 2020, Volume and Issue: 287(24), P. 5260 - 5283
Published: May 5, 2020
Neointimal hyperplasia (NIH) is a pathological process occurring in the blood vessel wall during atherosclerosis and in‐stent restenosis (ISR). Due to abundance of vascular smooth muscle cells (VSMCs) within neointimal lesions, VSMCs have long been considered as key cellular target preventing NIH. Noncoding RNA molecules such microRNA (miRNAs), noncoding (lncRNAs) circular RNAs (circRNAs) expressed offer unique therapeutic targets for tackling VSMC phenotype switching, proliferation, migration apoptosis processes responsible promoting In this review, we provide an extensive overview biology, highlighting most recent discoveries field lncRNAs circRNAs, with aim identifying molecular players that could be harnessed future interventions, our quest halt NIH disease.
Language: Английский
Citations
43
Heliyon, Journal Year: 2023, Volume and Issue: 9(5), P. e15451 - e15451
Published: April 19, 2023
Cardiomyocyte apoptosis has been characterized as one of the major mechanisms underlying doxorubicin (DOX)-induced cardiomyopathy. MicroRNA-21-5p (miR-21-5p) was reported to mitigate ischemia-induced cardiomyocyte and cardiac injury. However, our knowledge, functional role miR-21-5p in DOX-induced cardiomyopathy is unclear. In this study, we explored The expression level detected by quantitative real-time polymerase chain reaction (qRT-PCR). Dual luciferase reporter assay used verify potential target gene miR-21-5p. rate NRCMs TUNEL staining assay. Western blot analysis detect protein levels Bax, Bcl-2, Caspase3, cleaved-Caspase3 BTG2. For animal studies, mice were injected with AAV9-miR-21-5p or AAV9-Empty viruses, treated DOX at a dose 5 mg/kg per week through intraperitoneally administration. After 4 weeks treatment, subjected echocardiography measure left ventricular ejection fraction (EF) fractional shortening (FS). Results showed that upregulated both DOX-treated primary cardiomyocytes mouse heart tissues. Interestingly, enhanced inhibited oxidative stress, while decreased promoted stress. Furthermore, overexpression protected against mechanistic study indicated BTG2 anti-apoptotic effect could be overexpression. Conversely, inhibition rescued pro-apoptotic inhibitor. Taken together, prevent downregulating
Language: Английский
Citations
14
Frontiers in Aging, Journal Year: 2024, Volume and Issue: 5
Published: March 28, 2024
MicroRNAs (miRNAs) are short RNA molecules that not involved in coding for proteins. They have a significant function regulating gene expression after the process of transcription. Their participation several biological processes has rendered them appealing subjects investigating age-related disorders. Increasing data indicates miRNAs can be influenced by dietary variables, such as macronutrients, micronutrients, trace minerals, and nutraceuticals. This review examines influence factors nutraceuticals on regulation miRNA relation to aging. We examine present comprehension disruption illnesses emphasize possibility manipulation means prevention or treatment. Consolidating animal human research is essential validate significance control living organisms, despite abundance information already provided studies. elucidates complex interaction among miRNAs, nutrition, aging, offering valuable insights into promising areas further potential therapies
Language: Английский
Citations
6
Cellular Physiology and Biochemistry, Journal Year: 2017, Volume and Issue: 41(2), P. 510 - 518
Published: Jan. 1, 2017
Our previous microarray results identified numerous microRNAs (miRNAs), including miR-29b, that were differentially expressed in the serum of intracranial aneurysm (IA) patients. The current study aimed to investigate whether miR-29b downregulation IA could promote phenotypic modulation vascular smooth muscle cells (VSMCs) involved pathogenesis by activating ATG14-mediated autophagy.First, levels and autophagy related genes (ATGs) between patients normal subjects compared. Next, we modified level via lentivirus particles VSMCs examined effects on proliferation, migration, from a contractile phenotype synthetic phenotype, as well autophagy. Finally, binding 3'UTR ATG14 mRNA its expression analysed luciferase reporter assay Western blot, respectively.The was decreased, markers increased compared subjects. Knockdown significantly promoted proliferation migration and, more importantly, induced associated with activation, whereas overexpression showed opposite effects. demonstrated functional target gene miR-29b. Notably, knockdown siRNA apparently abrogated inhibition-mediated modulation.Downregulation directly autophagy, which is formation, growth rupture IAs.
Language: Английский
Citations
48
Frontiers in Cell and Developmental Biology, Journal Year: 2020, Volume and Issue: 8
Published: Feb. 25, 2020
The incidence of certain forms tumors has increased progressively in recent years and is expected to continue growing as life expectancy continues increase. Tumor-infiltrating NK cells may contribute develop an anti-tumor response. Optimized combinations different cancer therapies,, including cell-based approaches for targeting tumor cells, have the potential open new avenues immunotherapy. Functional inhibitory receptors on are needed prevent their attack healthy cells. Nevertheless disruption function increases cytotoxic capacity against MicroRNAs (miRNAs) small non-coding RNA molecules that target mRNA thus regulate expression genes involved development, maturation, effector functions Therapeutic strategies regulatory effects miRNAs improve efficiency Interestingly, emerging evidence points out some can, directly indirectly, control surface immune checkpoints or ligands This suggests a possible use context therapy. review provides current overview connections between regulation cell discusses these innovative biomarkers/targets
Language: Английский
Citations
36