Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 91, P. 103248 - 103248
Published: Dec. 31, 2024
Language: Английский
Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 238 - 238
Published: Jan. 23, 2024
Gut luminal dysbiosis and pathobiosis result in compositional biodiversified alterations the microbial host co-metabolites. The primary mechanism of bacterial evolution is horizontal gene transfer (HGT), acquisition new traits can be achieved through exchange mobile genetic elements (MGEs). Introducing genetically engineered microbes (GEMs) might break harmonized balance intestinal compartment. present objectives are: 1. To reveal role played by GEMs’ transfers changing landscape enteric microbiome eubiosis 2. expand on potential detrimental effects those changes human genome health. A search articles published PubMed/MEDLINE, EMBASE, Scielo from 2000 to August 2023 using appropriate MeSH entry terms was performed. exchanges induce multiple diseases. GEMs change long-term natural pro- or eukaryotic cell inhabitants. worldwide regulatory authority’s safety control not enough protect public Viability, biocontainment, many other aspects are only partially controlled harmful consequences for health should avoided. It important remember that prevention most cost-effective strategy primum non nocere focus.
Language: Английский
Citations
9
World Journal of Clinical Cases, Journal Year: 2024, Volume and Issue: 12(1), P. 1 - 8
Published: Jan. 2, 2024
Type 2 diabetes mellitus (T2DM) is a chronic metabolic disorder characterized by hyperglycemia and insulin resistance. The global prevalence of T2DM has reached epidemic proportions, affecting approximately 463 million adults worldwide in 2019. Current treatments for include lifestyle modifications, oral antidiabetic agents, therapy. However, these therapies may carry side effects fail to achieve optimal glycemic control some patients. Therefore, there growing interest the role gut microbiota more gut-targeted management T2DM. microbiota, which refers community microorganisms that inhabit human gut, been shown play crucial regulation glucose metabolism sensitivity. Alterations composition diversity have observed patients, with reduction beneficial bacteria an increase pathogenic bacteria. This dysbiosis contribute pathogenesis disease promoting inflammation impairing barrier function. Several developed modulate improve One potential approach use probiotics, are live confer health benefits host when administered adequate amounts. randomized controlled trials demonstrated certain such as Lactobacillus Bifidobacterium species, can sensitivity Mechanisms production short-chain fatty acids, improvement function, inflammation. Another therapy fecal transplantation (FMT), involves transfer material from healthy donor recipient. FMT used successfully treatment
Language: Английский
Citations
7
Published: Jan. 3, 2024
Gut luminal dysbiosis and pathobiosis result in compositional biodiversify alterations the microbial host co-metabolites. The main mechanism of bacterial evolution is horizontal gene transfer (HGT) acquisition new traits can be achieved through mobile genetic element (MGEs) exchange. Introducing genetically engineered microbes (GEMs) might break harmonized balance intestinal compartment. present objectives are: 1. To reveal role played by GEMs' transfers changing landscape enteric microbiome eubiosis 2. expand on potential detrimental effects those changes human genome health. A search articles published PubMed/MEDLINE, EMBASE, Scielo from 2000 to august 2023, using appropriate MeSH entry terms were performed. exchanges induce multiple diseases. GEMs change long-term natural pro- or eukaryotic cells inhabitants. worldwide regulatory authority's safety control not enough protect public Viability biocontainment many other aspects are only partially controlled harmful consequences for health should avoided. Always remember that prevention most cost- effective strategy "primum non nocere" name game.
Language: Английский
Citations
5
Insects, Journal Year: 2024, Volume and Issue: 15(5), P. 369 - 369
Published: May 19, 2024
Bees play a crucial role as pollinators, contributing significantly to ecosystems. However, the honeybee population faces challenges such global warming, pesticide use, and pathogenic microorganisms. Promoting bee growth using several approaches is therefore for maintaining their roles. To this end, bacterial microbiota well-known its native in supporting respects. Maximizing capabilities of these microorganisms holds theoretical potential promote bees. Recent advancements have made it feasible achieve enhancement through application genetic engineering. In review, we present roles gut symbionts promoting collectively summarize engineering that would be needed future applications. Particularly, has not been advanced, dominant symbiotic bacteria Snodgrassella alvi Gilliamella apicola are main focus paper, along with other species. Moreover, propose strategies will allow improvement listed gene targets modification further encourage use engineered growth.
Language: Английский
Citations
4
FEMS Microbiology Reviews, Journal Year: 2024, Volume and Issue: 48(6)
Published: Nov. 1, 2024
Abstract Microbes compete and cooperate with each other via a variety of chemicals circuits. Recently, to decipher, simulate, or reconstruct microbial communities, many researches have been engaged in engineering microbiomes bottom-up synthetic biology approaches for diverse applications. However, they separately focused on individual perspectives including genetic circuits, communications tools, microbiome engineering, promising The strategies coordinating ecosystems based different regulation circuits not systematically summarized, which calls more comprehensive framework the assembly communities. In this review, we summarize cross-talk orthogonal modules de novo assembling functional ecosystems, thus promoting further consortia-based First, review communication-based regulations among various communities from intra-species inter-species aspects. Then, are summarized at metabolites, transcription, translation, post-translation levels, respectively. Furthermore, give details better design optimize propose design-build-test-learn procedure function specification, chassis selection, interaction design, system build, performance test, modeling analysis, global optimization. Finally, current challenges opportunities discussed development application ecosystems.
Language: Английский
Citations
4
Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(50), P. 27679 - 27700
Published: Nov. 26, 2024
The burgeoning field of microbiome research has profoundly reshaped our comprehension human health, particularly highlighting the potential probiotics and fecal microbiota transplantation (FMT) as therapeutic interventions. While benefits traditional are well-recognized, efficacy mechanisms remain ambiguous, FMT's long-term effects still being investigated. Recent advancements in high-throughput sequencing have identified gut microbes with significant health benefits, paving way for next-generation (NGPs). These NGPs, engineered through synthetic biology bioinformatics, designed to address specific disease states enhanced stability viability. This review synthesizes current on NGP stability, challenges delivery, their applications preventing treating chronic diseases such diabetes, obesity, cardiovascular diseases. We explore physiological characteristics, safety profiles, action various strains while also addressing opportunities presented by integration into clinical practice. NGPs revolutionize microbiome-based therapies improve outcomes is immense, underscoring need further optimize ensure safety.
Language: Английский
Citations
4
PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown
Published: March 11, 2025
ABSTRACT Extracellular proteins are supposed to play crucial roles in the formation and structure of biofilms aggregates. However, often little is known about these proteins, particular for microbial communities. Here, we use two advanced metaproteomic approaches study extracellular proteome a granular Candidatus Accumulibacter enrichment as proxy communities that form solid granules, such those used biological wastewater treatment. Limited proteolysis whole granules metaproteome isolation from culture's supernatant successfully classified over 50% identified protein biomass be secreted. Moreover, structural sequence‐based classification 387 corresponding secreted biomass, with characteristics could aid aggregates, including filamentous, beta‐barrel containing, cell surface proteins. While various aggregate‐forming originated Ca . Accumulibacter, some associated other taxa. This suggests not only range different but also multiple organisms contribute biofilm formation. Therefore, obtained data provides resource exploring potentially support stability biofilms, whereas demonstrated can applied explore general.
Language: Английский
Citations
0
Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)
Published: April 18, 2025
Language: Английский
Citations
0
mSystems, Journal Year: 2025, Volume and Issue: unknown
Published: April 29, 2025
ABSTRACT The human gut microbiome’s role in colorectal cancer (CRC) pathogenesis has gained increasing recognition. This study aimed to delineate the microbiome characteristics that distinguish CRC patients from healthy individuals, while also evaluating influence of aging, through a comprehensive metagenomic approach. analyzed cohort 80 and matched controls, dividing participants into normal group, further categorized by age young, middle-aged, old-aged subgroups. Extensive sequencing fecal samples allowed for exploration both structural functional profiles microbiome, with findings validated an independent ensure robustness. Our results highlight notable differences composition between which exhibit age-dependent variations. Specifically, higher prevalence pathogenic bacteria, such as Bacteroides vulgatus , known drive inflammation carcinogenesis, was observed patients, alongside reduction beneficial microbes, including Lactobacillus . Functionally, CRC-associated showed increase pathways related DNA repair, cell cycle regulation, metabolic activities, Citrate Galactose metabolism, underscoring distinct microbial alterations could disease onset progression. These insights lay foundation future research microbiome-based diagnostics treatments CRC. IMPORTANCE underscores critical pathogenesis, particularly context aging. By identifying age-specific biomarkers associated CRC, our provide novel how activities discoveries pave way developing personalized diagnostic tools therapeutic strategies, potentially improving prevention treatment outcomes across different groups. Understanding these dynamics inform interventions targeting microbiota mitigate risk
Language: Английский
Citations
0
ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)
Published: Jan. 1, 2024
Abstract Tremendous advances in mass spectrometric and bioinformatic approaches have expanded proteomics into the field of microbial ecology. The commonly used spectral annotation method for metaproteomics data relies on database searching, which requires sample-specific databases obtained from whole metagenome sequencing experiments. However, creating these is complex, time-consuming, prone to errors, potentially biasing experimental outcomes conclusions. This asks alternative that can provide rapid orthogonal insights data. Here, we present NovoLign, a de novo pipeline performs sequence alignment sequences complete enables taxonomic profiling complex communities evaluates coverage searches. Furthermore, NovoLign supports creation reference searching ensure comprehensive coverage. We assessed false positive annotations using wide range silico data, including pure strains, laboratory enrichment cultures, synthetic communities, environmental communities. In summary, employs large-scale enable profiling, evaluation outcomes, databases. publicly available via: https://github.com/hbckleikamp/NovoLign.
Language: Английский
Citations
2