Processes,
Journal Year:
2024,
Volume and Issue:
12(11), P. 2411 - 2411
Published: Nov. 1, 2024
The
immobilization
of
enzymes,
especially
lipases,
presents
a
significant
challenge
in
contemporary
biotechnology
due
to
their
wide-ranging
application
industrial
processes.
Given
the
array
available
techniques
for
enzyme
immobilization,
this
study
aimed
immobilize
Candida
antarctica
B
(CALB)
lipase
within
silica
xerogel
and
sonogel
matrices
obtained
through
sol–gel
technique.
Polyethylene
glycol
(PEG)
was
incorporated
as
an
additive,
with
tetramethylorthosilicate
(TMOS)
serving
precursor.
This
assessed
operational
stability,
storage
thermal
properties
resulting
supports.
Results
revealed
that
both
supports,
supplemented
PEG,
maintained
stability
above
50%
throughout
365-day
period.
Moreover,
tests
demonstrated
support
could
be
reused
up
21
times,
while
exhibited
10
reuses.
Thermal
analysis
further
highlighted
reduction
deactivation
constant
elongation
half-life
time
These
observations
suggest
supports
effectively
shield
from
inactivation.
Overall,
these
findings
underscore
potential
utility
PEG-enhanced
various
applications,
providing
valuable
insights
into
operational,
storage,
stability.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(16)
Published: Feb. 20, 2024
Abstract
9,10‐Secosteroids
are
an
important
group
of
marine
steroids
with
diverse
biological
activities.
Herein,
we
report
a
chemoenzymatic
strategy
for
the
concise,
modular,
and
scalable
synthesis
ten
naturally
occurring
9,10‐secosteroids
from
readily
available
in
three
to
eight
steps.
The
key
feature
lies
utilizing
Rieske
oxygenase‐like
3‐ketosteroid
9α‐hydroxylase
(KSH)
as
biocatalyst
achieve
efficient
C9−C10
bond
cleavage
A‐ring
aromatization
tetracyclic
through
9α‐hydroxylation
fragmentation.
With
synthesized
9,10‐secosteroides,
structure–activity
relationship
was
evaluated
based
on
bioassays
terms
previously
unexplored
anti‐infective
activity.
This
study
provides
experimental
evidence
support
hypothesis
that
biosynthetic
pathway
which
formed
nature
shares
similar
fragmentation
cascade.
In
addition
development
biomimetic
approach
9,10‐secosteroid
synthesis,
this
highlights
great
potential
strategies
chemical
synthesis.
Frontiers in Pharmacology,
Journal Year:
2024,
Volume and Issue:
15
Published: May 31, 2024
Rheumatoid
arthritis
is
a
chronic
immunological
disease
leading
to
the
progressive
bone
and
joint
destruction.
Angiogenesis,
accompanied
by
synovial
hyperplasia
inflammation
underlies
Delaying
or
even
blocking
angiogenesis
has
emerged
as
an
important
target
of
RA
treatment.
Natural
medicines
long
history
treating
RA,
numerous
reports
have
suggested
that
natural
strong
inhibitory
activity
on
angiogenesis,
thereby
improving
progression
RA.
could
regulate
following
signaling
pathways:
HIF/VEGF/ANG,
PI3K/Akt
pathway,
MAPKs
NF-κB
PPARγ
JAK2/STAT3
etc.,
inhibiting
angiogenesis.
Tripterygium
wilfordii
Hook.
f.
(TwHF),
sinomenine,
total
glucoside
Paeonia
lactiflora
Pall.
Are
currently
most
representative
all
products
worthy
development
utilization.
In
this
paper,
main
factors
affecting
were
discussed
different
types
inhibit
systematically
summarized.
Their
specific
anti-angiogenesis
mechanisms
are
also
reviewed
which
aiming
provide
new
perspective
options
for
management
targeting
RSC Advances,
Journal Year:
2021,
Volume and Issue:
11(45), P. 28223 - 28270
Published: Jan. 1, 2021
This
review
article
discusses
developments
in
the
chemo-enzymatic
synthesis
of
alkaloids
since
2013,
showcasing
how
modern
methods
organic
and
biocatalysis
are
combined
to
establish
novel
routes
towards
these
important
natural
products.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(5), P. 2622 - 2622
Published: Feb. 27, 2022
Natural
products
are
usually
highly
complicated
organic
molecules
with
special
scaffolds,
and
they
an
important
resource
in
medicine.
structures
produced
by
enzymes,
this
is
still
a
challenging
research
field,
its
mechanisms
requiring
detailed
methods
for
elucidation.
Flavin
adenine
dinucleotide
(FAD)-dependent
monooxygenases
(FMOs)
catalyze
many
oxidation
reactions
chemo-,
regio-,
stereo-selectivity,
involved
the
synthesis
of
natural
products.
In
review,
we
introduce
different
FMOs,
classical
FAD
(C4a)-hydroperoxide
as
major
oxidant.
We
also
summarize
difference
between
FMOs
cytochrome
P450
(CYP450)
emphasizing
advantages
their
specificity
substrates.
Finally,
present
examples
FMO-catalyzed
Based
on
these
explanations,
review
will
expand
our
knowledge
powerful
well
implementation
effective
tools
biosynthesis.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(12), P. 8716 - 8726
Published: March 14, 2024
The
successful
biomimetic
or
chemoenzymatic
synthesis
of
target
natural
products
(NPs)
and
their
derivatives
relies
on
enzyme
discovery.
Herein,
we
discover
a
fungal
P450
BTG5
that
can
catalyze
the
formation
bicyclo[3.2.2]nonane
structure
through
an
unusual
two-step
mechanism
dimerization
cyclization
in
biosynthesis
beticolin
1,
whose
skeleton
connects
anthraquinone
moiety
xanthone
moiety.
Further
investigation
reveals
BTG5-T318
not
only
determines
substrate
selectivity
but
also
alters
catalytic
reactions,
which
allows
separation
reaction
to
two
individual
steps,
thereby
understanding
its
mechanism.
It
first
heterodimerization
undergoes
common
oxidation
process
for
P450s,
while
second
uncommon
formal
redox-neutral
step
is
proved
as
redox-mediated
reaction,
has
never
been
reported.
Therefore,
this
work
advances
our
P450-catalyzed
reactions
paves
way
expansion
diversity
class
NPs
synthetic
biology.
Marine Drugs,
Journal Year:
2025,
Volume and Issue:
23(2), P. 69 - 69
Published: Feb. 7, 2025
Marine
organisms
represent
a
source
of
unique
chemical
entities
with
valuable
biomedical
potentialities,
broad
diversity,
and
complexity.
It
is
essential
to
ensure
reliable
sustainable
supply
marine
natural
products
(MNPs)
for
their
translation
into
commercial
drugs
other
products.
From
structural
point
view
few
exceptions,
MNPs
pharmaceutical
importance
derive
from
the
so-called
secondary
metabolism
organisms.
When
production
strategies
rely
on
macroorganisms,
harvesting
or
culturing
coupled
extraction
procedures
frequently
remain
only
alternative
producing
these
compounds
an
industrial
scale.
Their
can
often
be
implemented
laboratory
scale
cultures
bacterial,
fungal,
microalgal
sources.
However,
diverse
approach,
combining
traditional
methods
modern
synthetic
biology
biosynthesis
strategies,
must
considered
invertebrate
MNPs,
as
they
are
usually
naturally
accumulated
in
very
small
quantities.
This
review
offers
comprehensive
examination
various
addressing
challenges
related
supply,
synthesis,
scalability.
also
underscores
recent
biotechnological
advancements
that
likely
transform
current
industrial-scale
manufacturing
pharmaceuticals
derived
