Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(2), P. 1085 - 1091
Published: Jan. 4, 2022
Small
extracellular
vesicles
(sEVs),
often
known
as
exosomes,
are
expected
to
be
a
promising
biomarker
for
the
early
diagnosis
of
cancer
because
they
carry
enriched
proteins
that
originated
from
parent
cells.
Profiling
surface
sEVs
offers
non-invasive
access
cancer.
However,
it
remains
challenging
simultaneously
detect
with
desired
sensitivity.
Herein,
dual
color
DNA
nanodevice
based
on
toehold-mediated
strand
displacement
signal
amplification
and
synchronous
fluorescence
technique
has
been
developed
simultaneous
analysis
high
As
nanodevice-based
system,
nanoconjugates
aptamer-magnetic
beads
can
recognize
lead
release
single-stranded
DNA.
Then,
released
trigger
amplification.
In
this
CD63
aptamer
MUC1
were
used
recognition
elements
detection
isolated
Under
optimal
conditions,
corresponding
determined
ultrasensitivity
by
method.
Also,
limits
two
67
particles/μL
37
MUC1.
Of
note,
as-constructed
method
applied
different
tumor
cell
lines
(SGC7901,
HepG2,
MCF-7
cells).
Furthermore,
system
successfully
precisely
identify
patients
healthy
people
serum
analysis.
The
strategy
demonstrates
great
potential
applications
in
Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(34), P. 11792 - 11799
Published: Aug. 19, 2021
The
exosome
has
emerged
as
a
promising
noninvasive
biomarker
for
the
early
diagnosis
of
cancer.
Therefore,
it
is
highly
desirable
to
develop
simple,
inexpensive,
and
user-friendly
biosensors
convenient,
sensitive,
quantitative
assay.
Herein,
we
developed
simple
cost-efficient
electrochemical
biosensor
by
combining
metal-organic
framework
(MOF)-functionalized
paper
screen-printed
electrode
(SPE)
portable,
ultrasensitive,
determination
cancer-derived
exosomes.
In
principle,
relied
on
recognition
Zr-MOFs
aptamer
initiate
hybridization
chain
reaction
(HCR)
formation
DNAzyme
signal
amplification.
Benefiting
from
high
amplification
ability
HCR,
label-free
paper-based
capable
ultrasensitive
assay
with
detection
limit
down
5
×
103
particles/mL,
which
superior
that
most
reported
methods.
Moreover,
proposed
possessed
advantages
low
cost,
operation,
sensitivity,
making
affordable
deliverable
point-of-care
(POC)
in
resource-limited
settings.
Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(8), P. 3669 - 3676
Published: Feb. 15, 2022
Common
homogeneous
electrochemical
(HEC)
sensors
usually
suffer
from
the
drawbacks
of
high
background
signal,
low
signal-to-noise
ratio,
and
even
false
positive
results
due
to
preaddition
electroactive
substances.
Thus,
it
is
necessary
develop
novel
HEC
based
on
in
situ
generation
substances
overcome
these
shortcomings,
which,
however,
underexplored.
In
this
work,
two-dimensional
(2D)
nanozymes,
i.e.,
cobalt-doped
2D
Ti3C2
MXene
nanosheets
(CMNSs),
with
excellent
peroxidase-like
properties
were
utilized
for
organophosphate
pesticides
(OPs)
detection.
The
CMNSs
synthesized
via
a
template-directed
wet
chemical
approach
displayed
outstanding
features
hydrophilia
water
dispersibility,
which
could
catalyze
oxidation
o-phenylenediamine
(OPD)
generate
significantly
increased
reduction
current.
Interestingly,
exhibited
unique
response
thiol
compounds
thus
employed
as
highly
efficient
catalysts
OPs
hydrolysis
acetylthiocholine
(ATCh)
form
thiocholine
catalyzed
by
acetylcholinesterase
(AChE)
inhibition
AChE
activity
OPs.
recovery
analysis
pakchoi
extract
solutions
ranged
97.4%
103.3%.
as-proposed
sensor
will
provide
new
way
development
high-performance
demonstrate
potential
applicability
determination
pesticide
residues
real
samples.
Journal of the American Chemical Society,
Journal Year:
2022,
Volume and Issue:
144(30), P. 13475 - 13486
Published: July 8, 2022
Breast
cancer
is
very
heterogeneous
and
the
most
frequently
diagnosed
worldwide,
precise
therapy
targeting
specific
subtypes
may
improve
survival
rates
of
breast
patients.
In
this
study,
we
designed
a
biomimetic
vesicle
by
camouflaging
catalytic
DNA
machinery
with
cell
membrane,
which
enabled
molecular
classification
circulating
exosomes
for
subtype-based
diagnosis
through
homotypic
recognition.
addition,
vesicles
specifically
targeted
fused
phenotypic
homology
manipulated
to
amplify
electrochemical
signaling
using
exosomal
RNA
as
an
endogenous
trigger.
The
prepared
MCF-7
cell-derived
membranes
were
shown
recognize
estrogen
receptor-positive
exhibited
low
detection
limit
557
particles
mL–1
microRNA-375
used
biomarker.
Furthermore,
MDA-MB-231
displayed
satisfactory
performance
in
analysis
triple-negative
potential
therapeutic
target,
PD-L1
mRNA,
Most
importantly,
cross-validation
experiments
confirmed
high
accuracy
selectivity
recognition-driven
subtyping
cancer.
When
applied
clinical
samples
patients,
demonstrated
feasibility
reliability
evaluating
features
stage-specific
monitoring
patients
because
signals
showed
positive
correlation
disease
progression.
Therefore,
work
provide
new
ideas
personalized
treatment
throughout
whole
process.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(4), P. 1529 - 1535
Published: March 24, 2023
Design
and
exploitation
of
high-property
multifunctional
emitters
for
achieving
immobilization-free,
label-free,
enzyme-free,
dual-mode
determination
microRNA
(miRNA)
with
high
sensitivity
selectivity
are
particularly
urgent
but
remain
a
huge
challenge.
Herein,
the
first
time,
we
reported
an
Ir3+-based
N-heterocyclic
complex,
namely,
IrPyPt,
ascertained
its
phosphorescence
electrochemiluminescence
(ECL)
dual-emission
behavior
in
homogeneous
solution,
discovered
turn-up
signal-off
ECL
response
to
G-quadruplex
DNA
over
other
DNAs.
Inspired
by
this,
IrPyPt
was
pioneered
as
emitter
combine
nucleic
acid
probes
(ON1
ON2)
developing
biosensor,
which
applied
highly
sensitive,
credible
analysis
miRNA-21
used
target
analyte.
triggered
hybridization
chain
reaction
ON1
ON2
generate
abundant
G-quadruplexes,
caught
yield
IrPyPt@G-quadruplex,
intramolecular
rotary
motion
inhibited
unable
diffuse
electrode,
contributing
boosted
emission
sharply
declined
emission.
With
this
design,
achieved
limits
detection
down
1.40
0.18
pM,
respectively.
Furthermore,
biosensor
triumphantly
determine
concentration
cancer
cell
lysates
thus
provided
great
potential
alternative
miRNA-related
diseases'
early
accurate
diagnosis.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(48), P. 17834 - 17842
Published: Nov. 21, 2023
Precise
and
sensitive
analysis
of
exosomal
microRNA
(miRNA)
is
great
importance
for
noninvasive
early
disease
diagnosis,
but
it
remains
a
challenge
to
detect
miRNA
in
human
blood
samples
because
their
small
size,
high
sequence
homology,
low
abundance.
Herein,
we
integrated
reliable
Pt–S
bond-mediated
three-dimensional
(3D)
DNA
nanomachine
magnetic
separation
homogeneous
electrochemical
strategy
the
detection
with
background
sensitivity.
The
3D
was
easily
prepared
via
facile
rapid
freezing
method,
capable
resisting
influence
biothiols,
thus
endowing
stability.
Notably,
as-developed
not
only
enabled
system
have
also
coupled
liposome
nanocarriers
synergistically
amplify
current
signal.
Consequently,
by
ingeniously
combining
multiple
signal-amplification
strategies
biosensing,
highly
successfully
achieved.
More
significantly,
good
anti-interference
ability,
as-proposed
method
could
effectively
discriminate
plasma
from
cancer
patients
healthy
subjects,
showing
potential
application
nondestructive
clinical
diagnosis
disease.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(2), P. 875 - 883
Published: Feb. 1, 2023
Due
to
the
heterogeneity
of
breast
cancer,
its
early
accurate
diagnosis
remains
a
challenge.
Exosomes
carry
abundant
genetic
materials
and
proteins
are
ideal
biomarkers
for
cancer
detection.
Herein,
ratiometric
surface-enhanced
Raman
scattering
(SERS)
biosensor
exosome
detection
was
constructed
using
regularly
arranged
Au@Ag
nanoparticles/graphene
oxide
(Au@Ag
NPs/GO)
substrate
with
4-nitrothiophenol
(4-NTP)
molecules
as
an
internal
standard.
Aptamers
two
overexpressed
(epithelial
cell
adhesion
molecule
human
epidermal
growth
factor
receptor
2)
were
linked
by
short
complementary
DNA
rhodamine
X
modified
at
3′-terminal
form
V-shaped
double-stranded
DNA,
which
attached
surface
NPs/GO
selective
recognition
cell-derived
exosomes.
In
presence
exosomes,
competitive
reaction
occurred,
resulting
in
formation
DNA/exosomes
complex,
separated
from
SERS
substrate.
The
signal
on
decreased
concentration
exosomes
increasing,
whereas
4-NTP
remained
stable.
strategy
provides
huge
electromagnetic
enhancement
adsorbing
sites
GO
layer,
achieving
wide
range
2.7
×
102
108
particles/mL
ultralow
limit
down
1.5
particles/mL,
without
requirement
any
nucleic
acid
amplification.
Particularly,
proposed
method
has
significant
applications
it
can
accurately
identify
clinical
serum
samples
differentiate
pancreatic
patients
healthy
individuals.
Small,
Journal Year:
2023,
Volume and Issue:
20(14)
Published: Nov. 20, 2023
Abstract
Nanozymes,
as
innovative
materials,
have
demonstrated
remarkable
potential
in
the
field
of
electrochemical
biosensors.
This
article
provides
an
overview
mechanisms
and
extensive
practical
applications
nanozymes
First,
definition
characteristics
are
introduced,
emphasizing
their
significant
role
constructing
efficient
sensors.
Subsequently,
several
common
categories
nanozyme
materials
delved
into,
including
metal‐based,
carbon‐based,
metal‐organic
framework,
layered
double
hydroxide
nanostructures,
discussing
Regarding
mechanisms,
two
key
roles
particularly
focused
biosensors:
selective
enhancement
signal
amplification,
which
crucially
support
sensor
performance.
In
terms
applications,
widespread
use
nanozyme‐based
biosensors
showcased
various
domains.
From
detecting
biomolecules,
pollutants,
nucleic
acids,
proteins,
to
cells,
providing
robust
means
for
high‐sensitivity
detection.
Furthermore,
insights
into
future
development
is
provided,
encompassing
improvements
optimizations
design
integration,
expansion
application
fields
through
interdisciplinary
collaboration.
conclusion,
this
systematically
presents
biosensors,
offering
valuable
references
prospects
research
field.
