Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(2), P. 1085 - 1091
Published: Jan. 4, 2022
Small
extracellular
vesicles
(sEVs),
often
known
as
exosomes,
are
expected
to
be
a
promising
biomarker
for
the
early
diagnosis
of
cancer
because
they
carry
enriched
proteins
that
originated
from
parent
cells.
Profiling
surface
sEVs
offers
non-invasive
access
cancer.
However,
it
remains
challenging
simultaneously
detect
with
desired
sensitivity.
Herein,
dual
color
DNA
nanodevice
based
on
toehold-mediated
strand
displacement
signal
amplification
and
synchronous
fluorescence
technique
has
been
developed
simultaneous
analysis
high
As
nanodevice-based
system,
nanoconjugates
aptamer-magnetic
beads
can
recognize
lead
release
single-stranded
DNA.
Then,
released
trigger
amplification.
In
this
CD63
aptamer
MUC1
were
used
recognition
elements
detection
isolated
Under
optimal
conditions,
corresponding
determined
ultrasensitivity
by
method.
Also,
limits
two
67
particles/μL
37
MUC1.
Of
note,
as-constructed
method
applied
different
tumor
cell
lines
(SGC7901,
HepG2,
MCF-7
cells).
Furthermore,
system
successfully
precisely
identify
patients
healthy
people
serum
analysis.
The
strategy
demonstrates
great
potential
applications
in
Deleted Journal,
Journal Year:
2023,
Volume and Issue:
1(2)
Published: March 8, 2023
Abstract
Extracellular
vesicles
(EVs)
play
an
important
role
in
many
physiological
processes.
Thus,
EV
analysis
has
a
great
value
for
the
understanding
of
mechanisms
underlying
disease
progress
or
diagnosis,
prognosis
and
therapy.
The
overlapped
physical
immune
properties
between
EVs
events
body
fluids,
as
well
phenotypic
heterogeneity
EVs,
require
efficient
isolation
methods.
unique
aptamers,
such
facile
modification
programmability,
make
them
easily
assembled
powerful
platforms
analysis.
can
also
be
used
vehicles
drug
delivery,
benefiting
from
homing
ability,
hypo‐immunogenicity,
strong
tolerance.
affinity
recognition
ability
to
targets
feature
single
stranded
DNA
aptamers
useful
promoting
targetability
delivery
nucleic
acid
drugs.
This
review
summarizes
recent
aptamer‐based
isolation,
analysis,
aptamer‐functionalized
therapeutics.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 2, 2025
Conventional
solid/liquid
electrochemical
interfaces
typically
encounter
challenges
with
impeded
mass
transport
for
poor
quantification
due
to
the
intricate
pathways
of
reactants
from
bulk
solution.
To
address
this
issue,
work
reports
an
innovative
approach
integrating
a
target-activated
DNA
framework
nanomachine
electrochemically
driven
metal-organic
(MOF)
conversion
self-sacrificial
biosensing.
The
presence
target
biomarker
serotonin
initiates
by
entropy-driven
circuit
form
cross-linked
nanostructure
and
subsequently
release
Fe-MOF
probe.
Acting
as
natural
metal
precursor
nanoconfined
source
reactant,
probe
is
converted
into
electroactive
Prussian
Blue
during
processes.
Taking
advantage
confinement
effect,
our
proposed
biosensor
exhibits
excellent
capability
detect
in
linear
range
1
pM
5
μM
remarkable
detection
limit
0.4
exceptional
specificity
against
other
interferents.
proof-of-concept
demonstration
clinical
serum
samples
patients
carcinoid
tumors
highlights
utility
complex
sample
analysis.
design
could
be
applied
high
potential
inspire
sensing
approaches,
holding
promise
applications
biomedical
research
disease
diagnosis.
Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 19, 2025
Precise
identification
and
analysis
of
multiple
protein
biomarkers
on
the
surface
breast
cancer
cell-derived
extracellular
vesicles
(BC-EVs)
are
great
significance
for
noninvasive
diagnosis
subtypes,
but
it
remains
a
major
challenge
owing
to
their
high
heterogeneity
low
abundance.
Herein,
we
established
CRISPR-based
homogeneous
electrochemical
strategy
near-zero
background
ultrasensitive
detection
BC-EVs.
To
realize
high-performance
capture
isolation
BC-EVs,
fluidity-enhanced
magnetic
nanoprobes
were
facilely
prepared.
After
capturing
AND
logic
gate-based
catalytic
hairpin
assembly
(CHA)
trans-cleavage
activity
CRISPR-Cas12a
against
signal
triggered
successively,
generating
significant
signal.
Notably,
as-developed
metal-mediated
could
efficiently
decrease
by
separation,
endowing
method
with
signal-to-noise
ratio.
Consequently,
ingeniously
integrating
DNA
CRISPR-CHA
amplification
dual
in
strategy,
precise
BC-EVs
was
successfully
achieved
through
simultaneous
specific
recognition
markers
surface.
More
importantly,
this
approach
effectively
discriminate
subgroups
clinical
serum
samples,
which
may
provide
opportunities
accurate
prognosis
evaluation
manner.
Nano Letters,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 15, 2025
Accurate
and
reliable
quantification
of
disease-related
biomolecules
is
essential
for
clinical
diagnosis.
In
this
study,
a
novel
electrochemical
approach
developed
based
on
target
triggered
DNA
nanostructural
switch
from
hairpin
dimer
to
double-stranded
wheel.
During
the
process,
species
get
closer
electrode
interface,
multiple
intramolecular
strand
displacements
are
beneficial
low
abundant
analysis.
addition,
use
nicking
endonuclease
mitigates
background
interference.
This
strategy
enables
highly
sensitive
selective
miRNA.
A
linear
relationship
established
between
peak
current
intensity
logarithm
miRNA
concentration
within
range
0.1
20
fM.
method
also
demonstrates
high
accuracy
in
analysis
samples,
holding
great
potential
nanotechnology
diagnostic
applications.
ACS Applied Materials & Interfaces,
Journal Year:
2021,
Volume and Issue:
13(33), P. 38931 - 38946
Published: Aug. 10, 2021
With
the
continuous
development
of
DNA
nanotechnology,
various
spatial
structures
and
assembly
techniques
emerge.
Hybridization
chain
reaction
(HCR)
is
a
typical
example
with
exciting
features
bright
prospects
in
biosensing,
which
has
been
intensively
investigated
past
decade.
In
this
Spotlight
on
Applications,
we
summarize
principles
conventional
HCR
some
novel
forms
linear/nonlinear
HCR.
advantages
like
great
kinetics,
facile
operation,
an
enzyme-free
isothermal
reaction,
these
strategies
can
be
integrated
most
mainstream
reporters
(e.g.,
fluorescence,
electrochemistry,
colorimetry)
for
ultrasensitive
detection
abundant
targets.
Particularly,
select
several
representative
studies
to
better
illustrate
ideas
performances
strategies.
Theoretical
practical
utilities
are
confirmed
range
biosensing
applications.
end,
deep
discussion
provided
about
challenges
future
tasks
field.
Analytical Chemistry,
Journal Year:
2021,
Volume and Issue:
93(32), P. 11251 - 11258
Published: Aug. 9, 2021
Photoelectrochemical
(PEC)
sensors
are
relatively
new
sensing
platforms
with
high
detection
sensitivity
and
low
cost.
However,
the
current
PEC
biosensors
dependent
on
ultraviolet
or
visible
light
as
exciting
resource
cause
injuries
to
biological
samples
systems,
which
restrains
applications
in
complicated
matrixes.
Herein,
a
near-infrared
(NIR)-initiated
biosensor
based
NaYF4:Yb,Tm@NaYF4@TiO2@CdS
(csUCNRs@TiO2@CdS)
was
constructed
for
sensitive
of
acute
myocardial
infarction
(AMI)-related
miRNA-133a
an
immobilization-free
format
coupled
hybridization
chain
reaction
redox
circle
signal
amplification
strategy.
A
low-energy
980
nm
NIR
incident
laser
converted
300–480
excite
adjacent
TiO2@CdS
photosensitive
shell
generate
photocurrent
by
NaYF4:Yb,Tm@NaYF4
upconversion
nanorods.
Also,
magnetic
beads
were
employed
homogeneous
determination
target
reduce
recognition
steric
hindrance
improve
sensitivity.
The
response
positively
correlated
level
ascorbic
acid
energy
donor
consume
photoacoustic
holes
produced
surface
csUCNRs@TiO2@CdS,
generated
alkaline
phosphatase
catalyzation
regenerated
tris(2-carboxyethyl)
phosphine
reduction
upon
appearance
miRNA-133a.
Exerting
NIR-light-driven
strategy,
as-constructed
displayed
linearly
selective
limit
36.12
aM.
More
significantly,
assay
method
provided
concept
strategy
driven
detect
diverse
biomarkers
pronounced
sensitivity,
stability,
photodamage.
