Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Language: Английский

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Single-Cell Liquid Biopsy of Lung Cancer: Ultra-Simplified Efficient Enrichment of Circulating Tumor Cells and Hand-Held Fluorometer Portable Testing

ACS Nano, Journal Year: 2024, Volume and Issue: 18(6), P. 5017 - 5028

Published: Feb. 2, 2024

Herein, we propose a paper-based laboratory via enzyme-free nucleic acid amplification and nanomaterial-assisted cation exchange reactions (CERs) assisted single-cell-level analysis (PLACS). This method allowed for the rapid detection of mucin 1 trace circulating tumor cells (CTCs) in peripheral blood lung cancer patients. Initially, an independently developed requiring one centrifuge, two reagents (lymphocyte separation solution erythrocyte lysate), three-step, 45 min sample pretreatment was employed. The core approach consisted competitive selective identifications: copper sulfide nanoparticles (CuS NPs) to C–Ag+–C Ag+, dual quantum dots (QDs) Cu2+ CuS NPs. To facilitate multimodal point-of-care testing (POCT), integrated visualization, test strip length reading, self-developed hand-held fluorometer readout. These methods were detectable down ag/mL concentration single-cell level. Forty-seven clinical samples assayed by fluorometer, yielding 94% (30/32) sensitivity 100% (15/15) specificity with area under curve (AUC) 0.945. Nine 15 retested respectively, AUC 0.95. All results consistent imaging folate receptor (FR)-PCR kit findings, supporting its potential early diagnosis postoperative monitoring.

Language: Английский

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Buoyant Metal–Organic Framework Corona-Driven Fast Isolation and Ultrasensitive Profiling of Circulating Extracellular Vesicles

ACS Nano, Journal Year: 2024, Volume and Issue: 18(22), P. 14569 - 14582

Published: May 23, 2024

Accurately assaying tumor-derived circulating extracellular vesicles (EVs) is fundamental in noninvasive cancer diagnosis and therapeutic monitoring but limited by challenges efficient EV isolation profiling. Here, we report a bioinspired buoyancy-driven metal-organic framework (MOF) corona that leverages on-bubble coordination dual-encoded surface-enhanced Raman scattering (SERS) nanotags to streamline rapid ultrasensitive profiling of plasma EVs single assay for diagnostics. This integrated bubble-MOF-SERS (IBMsv) allows barnacle-like high-density adhesion MOFs on self-floating bubble surface enable fast (2 min, near 90% capture efficiency) tumor via enhanced EV-MOF binding. Also, IBMsv harnesses four-plexed SERS profile the captured protein markers at single-particle level. Such sensitive multiplexed across five types, revealing heterogeneous expression patterns. Furthermore, enables pilot clinical cohort (

Language: Английский

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Small extracellular vesicles: Non-negligible vesicles in tumor progression, diagnosis, and therapy

Cancer Letters, Journal Year: 2023, Volume and Issue: 580, P. 216481 - 216481

Published: Nov. 15, 2023

Small extracellular vesicles (sEVs) such as exosomes are nanoscale membranous particles (<200 nm) that have emerged crucial targets for liquid biopsy and promising drug delivery vehicles. They play a significant role in tumor progression intercellular messengers. can serve biomarkers diagnosis carriers cancer treatment. This article reviews recent studies on sEVs oncology explores their potential Following tumorigenesis, the microenvironment (TME) circulatory system undergo modifications to regulate various events TME, including angiogenesis, epithelial-mesenchymal transition (EMT), immunity, with either pro- or anti-tumor effects. been investigated use diagnostic prognostic variety of tumors, lung cancer, melanoma, breast prostate hepatocellular carcinoma. be used therapy by packaging drugs proteins into them through pre- post-isolation modification techniques. The clinical trials also summarized. Finally, challenges described possible approaches tackling suggested. Overall, will advance precision medicine has shown great applications.

Language: Английский

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Bacterial extracellular vesicles: A position paper by the microbial vesicles task force of the Chinese society for extracellular vesicles

Deleted Journal, Journal Year: 2023, Volume and Issue: 1(3)

Published: July 1, 2023

Abstract Recently, the interest in extracellular vesicles released by bacteria has rapidly increased. Bacterial (BEVs) have been involved bacteria‐bacteria and bacteria‐host interactions, which strengthen health or bring about various pathologies. However, BEV separation, characterization, functional studies require establishment of guidelines further optimization order to stimulate development science research a following successful transformation into clinical applications. This position paper is authored Microbial Vesicles Task Force Chinese Society for Extracellular (CSEV) composed experienced medical laboratory specialists, microbiologists, virologists, biologists material who are actively engaged research. Herein, we present concise description discover challenges critical gaps current BEV‐based analyses Finally, also offer suggestions considerations improve experimental reproducibility interoperability promote progress field.

Language: Английский

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A novel electrochemiluminescent cytosensor using 3D multivalent aptamer recognition and covalent organic frameworks-associated DNA walker for highly efficient capture and detection of rare CTCs

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 481, P. 148606 - 148606

Published: Jan. 6, 2024

Language: Английский

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Portable Aptasensor Based on Parallel Rolling Circle Amplification for Tumor‐Derived Exosomes Liquid Biopsy

Advanced Science, Journal Year: 2024, Volume and Issue: 11(32)

Published: June 26, 2024

Here, a separation-free and label-free portable aptasensor is developed for rapid sensitive analysis of tumor-derived exosomes (TEXs). It integrated parallel rolling circle amplification (RCA) reaction, selective binding metal ions or small molecules to nucleic acid-specific conformations, low-cost, highly handheld fluorometer. Lung cancer, example, targeted with two typical biomarkers (mucin 1 programmed cell death ligand (PD-L1)) on its exosomes. The affinity aptamers the targets modulated amount RCA products (T-Hg

Language: Английский

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Plant-derived vesicle-like nanoparticles: A new tool for inflammatory bowel disease and colitis-associated cancer treatment

Molecular Therapy, Journal Year: 2024, Volume and Issue: 32(4), P. 890 - 909

Published: Feb. 18, 2024

Language: Английский

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Bacterial extracellular vesicles in the initiation, progression and treatment of atherosclerosis

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: Jan. 22, 2025

Atherosclerosis is the primary cause of cardiovascular and cerebrovascular diseases. However, current anti-atherosclerosis drugs have shown conflicting therapeutic outcomes, thereby spurring search for novel effective treatments. Recent research indicates crucial involvement oral gastrointestinal microbiota in atherosclerosis. While gut metabolites, such as choline derivatives, been extensively studied reviewed, emerging evidence suggests that bacterial extracellular vesicles (BEVs), which are membrane-derived lipid bilayers secreted by bacteria, also play a significant role this process. BEVs host-microbiota interactions remains insufficiently explored. This review aims to elucidate complex communication mediated along gut-heart axis. In review, we summarize knowledge on BEVs, with specific focus how pathogen-derived contribute promotion atherosclerosis, well from symbionts probiotics may mitigate its progression. We explore potential challenges associated engineered prevention treatment Finally, discuss benefits using atherosclerosis diagnosis treatment, propose future directions address these issues.

Language: Английский

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Exosome isolation and characterization for advanced diagnostic and therapeutic applications

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101613 - 101613

Published: Feb. 25, 2025

Language: Английский

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