Promising Solutions to Address the Non-Specific Adsorption in Biosensors Based on Coupled Electrochemical-Surface Plasmon Resonance Detection DOI Creative Commons
Alina Vasilescu, Szilveszter Gáspár, Mihaela Gheorghiu

et al.

Chemosensors, Journal Year: 2025, Volume and Issue: 13(3), P. 92 - 92

Published: March 5, 2025

Nonspecific adsorption (NSA) impacts the performance of biosensors in complex samples. Coupled electrochemical–surface plasmon resonance (EC-SPR) offer interesting opportunities to evaluate NSA. This review details main solutions minimize fouling electrochemical (EC), surface (SPR) and EC-SPR biosensors. The discussion was centered on blood, serum milk as examples matrices. Emphasis placed antifouling coatings, NSA evaluation protocols universal functionalization strategies obtain In last 5 years, various coatings were developed for EC biosensors, including new peptides, cross-linked protein films hybrid materials. Due comparatively much more scarce literature, SPR extended early 2010s. analysis revealed a wide range materials with tunable conductivity, thickness functional groups that can be tested future EC-SPR. high-throughput screening materials, molecular simulations machine learning-assisted evaluations will even further widen available minimization NSA’s impact analytical signal is moreover facilitated by unique sensing mechanisms associated bioreceptor or particularities detection method. It hoped this encourage research field

Language: Английский

Biomimic Nanozymes with Tunable Peroxidase-like Activity Based on the Confinement Effect of Metal–Organic Frameworks (MOFs) for Biosensing DOI
Nuanfei Zhu,

Chengbin Liu,

Rui Liu

et al.

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(11), P. 4821 - 4830

Published: March 9, 2022

Biomimic nanozymes coassembled by peptides or proteins and small active molecules provide an effective strategy to design attractive nanozymes. Although some promising have been reported, rational regulation for higher catalytic activity of biomimic remains challenging. Hence, we proposed a novel nanozyme encapsulating the coassembly hemin/bovine serum albumin (BSA) in zeolite imidazolate frameworks (ZIF-8) achieve controllable tailoring peroxidase-like via confinement effect. The assembly Hemin@BSA was inspired structure horseradish peroxidase (HRP), which hemin served as cofactor surrounded BSA blocking pocket construct favorable hydrophobic space substrate enrichment. Benefiting from effect, ZIF-8 with porous intracavity identified ideal outer layer accelerate transport internal circulation catalysis, significantly enhancing its activity. Especially, precise encapsulation could also prevent it decomposition harsh environments rapid crystallization around form protective shell. Based on improved Hemin@BSA@ZIF-8, several applications were successfully performed sensitive detection including H2O2, glucose, bisphenol A (BPA). Satisfactory results highlight that using encapsulate offers very successful improve stability nanozymes, broadening potential application biocatalytic metal-organic (MOFs).

Language: Английский

Citations

116
Comprehensive review on the electrochemical biosensors of different breast cancer biomarkers DOI
Islam M. Mostafa, Ye Tian,

Saima Anjum

et al.

Sensors and Actuators B Chemical, Journal Year: 2022, Volume and Issue: 365, P. 131944 - 131944

Published: April 25, 2022

Language: Английский

Citations

71
Polymer Gels: Classification and Recent Developments in Biomedical Applications DOI Creative Commons
Mariana Chelu, Adina Magdalena Musuc

Gels, Journal Year: 2023, Volume and Issue: 9(2), P. 161 - 161

Published: Feb. 17, 2023

Polymer gels are a valuable class of polymeric materials that have recently attracted significant interest due to the exceptional properties such as versatility, soft-structure, flexibility and stimuli-responsive, biodegradability, biocompatibility. Based on their properties, polymer can be used in wide range applications: food industry, agriculture, biomedical, biosensors. The utilization different medical industrial applications requires better understanding formation process, factors which affect gel’s stability, structure-rheological relationship. present review aims give an overview gels, classification gels’ highlight important features, recent development biomedical applications. Several perspectives future advancement hydrogel offered.

Language: Английский

Citations

60
Electrochemical biosensors represent promising detection tools in medical field DOI Creative Commons
Li Zhang, Wenqiang Guo,

Chenrui Lv

et al.

Advanced Sensor and Energy Materials, Journal Year: 2023, Volume and Issue: 2(4), P. 100081 - 100081

Published: Oct. 29, 2023

In recent years, there has been a growing demand for rapid detection methods, leading to the proliferation of biosensors, particularly electrochemical which have garnered substantial attention from researchers. The fusion biosensors with advanced sensing technology given rise diverse array characterized by their capabilities and heightened sensitivity. Consequently, an imperative exists comprehensively recapitulate advances in especially within realm clinical medicine. This review aims elucidate applications delineate future development prospects, facilitating more profound comprehension further progression this field. Specifically, we will examine utilization medicine, encompassing roles point-of-care testing, disease diagnosis, therapeutic monitoring. Moreover, spotlight emerging trends prospective innovations, such as evolution nanostructured sensors portable devices. By providing comprehensive overview potential significantly contribute advancement field serve catalyst research into innovative tools.

Language: Английский

Citations

51
Electrochemical Biosensors for the Analysis of Breast Cancer Biomarkers: From Design to Application DOI
Jing Le,

Chongyu Xie,

Qianqian Li

et al.

Analytical Chemistry, Journal Year: 2021, Volume and Issue: 94(1), P. 269 - 296

Published: Dec. 2, 2021

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTElectrochemical Biosensors for the Analysis of Breast Cancer Biomarkers: From Design to ApplicationLe JingLe JingState Key Laboratory Biogeology Environmental Geology, Engineering Research Center Nano-Geomaterials Ministry Education, Faculty Materials Science and Chemistry, China University Geosciences, Wuhan 430074, ChinaMore by Le JingView Biography, Chongyu XieChongyu XieState XieView Qianqian LiQianqian LiState LiView Meiqing YangMeiqing YangState YangView Shaoguang Li*Shaoguang China*Email: [email protected]More Hui LiHui Biographyhttps://orcid.org/0000-0001-7849-9735, Fan XiaFan XiaState XiaView Biographyhttps://orcid.org/0000-0001-7705-4638Cite this: Anal. Chem. 2022, 94, 1, 269–296Publication Date (Web):December 2, 2021Publication History Published online2 December 2021Published inissue 11 January 2022https://pubs.acs.org/doi/10.1021/acs.analchem.1c04475https://doi.org/10.1021/acs.analchem.1c04475review-articleACS PublicationsCopyright © 2021 American Chemical SocietyRequest reuse permissionsArticle Views5918Altmetric-Citations33LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biomarkers,Biotechnology,Cancer,Differential pulse voltammetry,Sensors Get e-Alerts

Language: Английский

Citations

85
Enabling Multiplexed Electrochemical Detection of Biomarkers with High Sensitivity in Complex Biological Samples DOI
Sharma T. Sanjay, Pawan Jolly, Nolan Durr

et al.

Accounts of Chemical Research, Journal Year: 2021, Volume and Issue: 54(18), P. 3529 - 3539

Published: Sept. 3, 2021

ConspectusThe ability to perform multiplexed detection of various biomarkers within complex biological fluids in a robust, rapid, sensitive, and cost-effective manner could transform clinical diagnostics enable personalized healthcare. Electrochemical (EC) sensor technology has been explored as way address this challenge because it does not require optical instrumentation is readily compatible with both integrated circuit microfluidic technologies; yet approach had little impact viable commercial bioanalytical tool date. The most critical limitation hindering their application the fact that EC sensors undergo rapid biofouling when exposed samples (e.g., blood, plasma, saliva, urine), leading loss sensitivity selectivity. Thus, break through barrier, we must solve problem.In response challenge, our group developed low-cost nanocomposite-based antifouling coating for enables unprecedented performance terms biomarker signal compared reported literature. bioinspired nanoporous composite contains conductive nanomaterials, including gold nanowires (AuNWs), carbon nanotubes (CNTs), or reduced graphene oxide nanoflakes (rGOx). Each study progressively evolved provide increasing while simplifying process flow, reducing time, decreasing cost. For example, after successfully developing semipermeable nanocomposite containing AuNWs cross-linked bovine serum albumin (BSA) using glutaraldehyde, replaced nanomaterials oxide, cost by 100-fold maintaining similar transduction properties. We, subsequently, localized heat-induced method significantly improved efficiency drop-casting occurs time <1 min (at least 3 orders magnitude faster than state-of-the-art). Moreover, resulting coated electrodes can be stored at room temperature 5 months still maintain full specificity. Importantly, showed excellent activity against fluids, serum, whole urine, saliva.To affinity-based sensing multiple simultaneously, have then employed sandwich enzyme-linked immunosorbent assay (ELISA) format which substrate enzyme bound secondary antibody precipitates locally molecular binding site above electrode surface. Using platform, demonstrated ultrasensitive wide range from biomarkers, single multiplex formats (N = 4) times 37 15 system. These biosensors demonstrate vast potential solving problem, how clinically important diagnostic applications. This Account reviews surface chemistry sensor-based biodetection places context innovative contributions made other researchers field. We are optimistic future iterations these systems will change testing done, where carried out, future.

Language: Английский

Citations

73
Copper Peroxide Nanodots Encapsulated in a Metal–Organic Framework for Self-Supplying Hydrogen Peroxide and Signal Amplification of the Dual-Mode Immunoassay DOI

Dali Wei,

Dinghui Xiong, Nuanfei Zhu

et al.

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(38), P. 12981 - 12989

Published: Sept. 16, 2022

The necessary step of directly adding hydrogen peroxide (H2O2) into the detection system in traditional immunoassays hampers their applications as a portable device for point-of-care analysis due to unstable liquid form H2O2. Herein, strategy self-supplying H2O2 and signal amplification triggering by copper nanodots encapsulated (CPNs) metal–organic frameworks (ZIF-8) was proposed an immunoassay dual-signal bisphenol A (a typical emerging organic pollutant), which further fabricated lab-in-a-tube integrated with smartphone sensing platform. CPNs@ZIF-8 modified on antibody against A; after competitive binding analytes, coating antigens, antibodies, released Cu2+ from CPNs under acidic condition will trigger Fenton-like reaction generate ·OH oxidization TMB; meanwhile, could quench fluorescence GSH-Au NCs, resulting dual-mode signals measurements. Most importantly, high stability undertaken CPNs, remarkably increased molecule (CPN) loading ascribed excellent capacity (ZIF-8). In addition, good recoveries were obtained colorimetric/fluorescent strategy. constructed demonstrated great potential universal platform rapid various environmental contaminants using corresponding antibodies relying its performance satisfactory stability, sensitivity, accuracy.

Language: Английский

Citations

64
Peptide-Based Hydrogels: New Materials for Biosensing and Biomedical Applications DOI Open Access
Roya Binaymotlagh, Laura Chronopoulou, Farid Hajareh Haghighi

et al.

Materials, Journal Year: 2022, Volume and Issue: 15(17), P. 5871 - 5871

Published: Aug. 25, 2022

Peptide-based hydrogels have attracted increasing attention for biological applications and diagnostic research due to their impressive features including biocompatibility biodegradability, injectability, mechanical stability, high water absorption capacity, tissue-like elasticity. The aim of this review will be present an updated report on the advancement peptide-based activity in recent years field anticancer drug delivery, antimicrobial wound healing materials, 3D bioprinting tissue engineering, vaccines. Additionally, biosensing key group discussed mainly focusing cancer detection.

Language: Английский

Citations

61
Antifouling zwitterionic peptide hydrogel based electrochemical biosensor for reliable detection of prostate specific antigen in human serum DOI

Qiushu Du,

Wenqi Wang,

Xianghua Zeng

et al.

Analytica Chimica Acta, Journal Year: 2022, Volume and Issue: 1239, P. 340674 - 340674

Published: Nov. 25, 2022

Language: Английский

Citations

41
A Wearable Electrochemical Sensor Based on Anti-Fouling and Self-Healing Polypeptide Complex Hydrogels for Sweat Monitoring DOI

Xiujuan Qiao,

Y. Cai,

Zhaoyang Kong

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(7), P. 2834 - 2842

Published: July 20, 2023

Although continuous monitoring of constituents in complex sweat is crucial for noninvasive physiological evaluation, biofouling on the sensor surface and inadequate flexible self-healing materials restrict its applications. Herein, a fully strong anti-biofouling polypeptide hydrogel (AuNPs/MoS2/Pep hydrogel) with excellent electrochemical performances was created. The anti-fouling fabricated based AuNPs/MoS2/Pep to address these issues. It found that designed form network structure carried abundant hydrophilic groups, resulting superior properties 30 min even long-term stability undiluted human sweat. In addition, SEM, TEM, UV, XPS, infrared spectrogram demonstrated binding force π-π stacking between MoS2 naphthalene groups peptide endowed an property. Furthermore, preserved wearable device function continuously uric acid (UA) ascorbic (AA) situ. This novel high ability, property, sensitive selective analytical capability describes new opportunity health

Language: Английский

Citations

35