Paper‐based Wearable Electrochemical Sensors: A New Generation of Analytical Devices

Electroanalysis, Journal Year: 2022, Volume and Issue: 35(1)

Published: June 9, 2022

Abstract Over the past few years, emergence of electrochemical wearable sensors has attracted considerable attention because their promising application in point‐of‐care testing due to some features such as high sensitivity, simplicity, miniaturization, and low fabrication cost. Recent developments new approaches innovative substrates have resulted able real‐time on‐body measurements. Wearable also been combined with paper‐based directly used on human skin for different applications non‐invasive analyses. Furthermore, enable monitoring analytes biofluids without complex procedures, pre‐treatment or sample manipulation. The coupling IoT various data collection handling remote resource‐limited conditions. This mini‐review presents significant advances developing devices, sampling, collection, connection protocols, power sources, discusses critical challenges higher performance this field. We present an overview paper intelligent substrate discuss advantages drawbacks. Lastly, conclude by highlighting future diagnostics measurements multiplexed detection biomarkers simultaneously, reducing cost time classical analysis provide fast complete overall physiological conditions wearer.

Language: Английский

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Development of Nucleic‐Acid‐Based Electrochemical Biosensors for Clinical Applications

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(50)

Published: Sept. 30, 2022

Nucleic acids are remarkable molecules. In addition to Watson-Crick base pairing, the different structural motifs of these molecules can bind non-nucleic acid targets or catalyze chemical reactions. Additionally, nucleic easily modified with functional groups. These properties make acids, particularly DNA, ideally suited for use in electrochemical biosensors, both as biorecognition elements and redox reporter probes. this Minireview, we will review historical evolution probes biosensors. We then specific examples nucleic-acid-based biosensors that have been evaluated clinical areas infectious disease, cancer, cardiovascular health.

Language: Английский

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Nanomaterial-based microfluidic systems for cancer biomarker detection: Recent applications and future perspectives

TrAC Trends in Analytical Chemistry, Journal Year: 2022, Volume and Issue: 158, P. 116835 - 116835

Published: Nov. 18, 2022

Language: Английский

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COF-Coated Microelectrode for Space-Confined Electrochemical Sensing of Dopamine in Parkinson’s Disease Model Mouse Brain

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(43), P. 23727 - 23738

Published: Oct. 20, 2023

Parkinson's disease (PD) is a progressive neurodegenerative disorder causing the loss of dopaminergic neurons in substantia nigra and drastic depletion dopamine (DA) striatum; thus, DA can act as marker for PD diagnosis therapeutic evaluation. However, detecting brain not easy because its low concentration difficulty sampling. In this work, we report fabrication covalent organic framework (COF)-modified carbon fiber microelectrode (cCFE) that enables real-time detection mouse thanks to outstanding antibiofouling antichemical fouling ability, excellent analytical selectivity, sensitivity offered by COF modification. particular, inhibit polymerization on electrode (namely, chemical fouling) spatially confining molecular conformation electrochemical oxidation DA. The cCFE stably continuously work detect monitor variation concentration. Furthermore, it was combined with levodopa administration devise closed-loop feedback mode therapy, which monitors model instruct dose injection time levodopa, allowing customized medication improve efficacy meanwhile avoid adverse side effects. This demonstrates fascinating properties fabricating sensors vivo bioanalysis. We believe structural tunability diversity will offer enormous promise selective neurotransmitters brain.

Language: Английский

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Programmable DNA-Fueled Electrochemical Analysis of Lung Cancer Exosomes

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(24), P. 8748 - 8755

Published: June 1, 2022

Molecular diagnostics devoted to discover and monitor new biomarkers is gaining increasing attention in clinical diagnosis. In this work, a programmable DNA-fueled electrochemical analysis strategy designed for the determination of an emerging biomarker lung cancer, PD-L1-expressing exosomes. Specifically, exosomes are first enriched onto magnetic beads functionalized with PD-L1 antibody able interact cholesterol-modified hairpin templates. Then, DNA synthesis starts from template-triggered primer exchange reaction generates large number extension products activate trans-cleavage activity CRISPR-Cas12a. After that, CRISPR-Cas12a-catalyzed random cleavage boosts degradation methylene blue-labeled signaling strands, so electro-active blue molecules can be cucurbit[7]uril-modified electrode quantitative determination. Our method demonstrates high sensitivity specificity toward range 103 109 particles mL-1 low detection limit 708 mL-1. When applied samples, our reveals elevated level circulating cancer patients, especially those at advanced stages. Therefore, may provide insight into liquid biopsy better implementation immunotherapy future.

Language: Английский

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Affinity-based electrochemical sensors for biomolecular detection in whole blood

Analytical and Bioanalytical Chemistry, Journal Year: 2023, Volume and Issue: 415(18), P. 3983 - 4002

Published: March 14, 2023

Abstract The detection and/or quantification of biomarkers in blood is important for the early detection, diagnosis, and treatment a variety diseases medical conditions. Among different types sensors detecting molecular biomarkers, such as proteins, nucleic acids, small-molecule drugs, affinity-based electrochemical offer advantages high analytical sensitivity specificity, fast times, simple operation, portability. However, biomolecular whole challenging due to its highly complex matrix, necessitating sample purification (i.e., centrifugation), which involves use bulky, expensive equipment tedious sample-handling procedures. To address these challenges, various strategies have been employed, purifying directly on sensor, employing micro-/nanoparticles enhance signal, coating electrode surface with blocking agents reduce nonspecific binding, improve performance without requiring pre-processing steps or laboratory equipment. In this article, we present an overview sensor technologies that employ blood. Graphical abstract

Language: Английский

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Hydrogel-Coated Microelectrode Resists Protein Passivation of In Vivo Amperometric Sensors

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(6), P. 3390 - 3397

Published: Feb. 1, 2023

Passivation of electrodes caused by nonspecific adsorption protein can dramatically reduce sensing sensitivity and accuracy, which is a great challenge for in vivo neurochemical monitoring. However, most antipassivation strategies are not suitable to carbon fiber microelectrodes (CFMEs) measurement, these methods also do work on electrochemical biosensors that fix biometric elements. In this study, we demonstrate chitosan hydrogel-coated avoid the current passivation surface because hydrogel prepared local pH gradient hydrogen evolution reaction has three-dimensional networks containing large amounts water. The highly hydrophilic structure only forms biocompatible interface confine enzymes but keeps fast mass transfer analytes, such as dopamine, ascorbic acid, glucose. consistency precalibration postcalibration sensor enables amperometric detection both electroactive species based their redox property electroinactive enzyme. This study provides simple versatile strategy constitute an resist complex biological environment brain.

Language: Английский

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Nanoarchitectonics-based electrochemical aptasensors for highly efficient exosome detection

Science and Technology of Advanced Materials, Journal Year: 2024, Volume and Issue: 25(1)

Published: April 22, 2024

Exosomes, a type of extracellular vesicles, have attracted considerable attention due to their ability provide valuable insights into the pathophysiological microenvironment cells from which they originate. This characteristic implicates potential use as diagnostic disease biomarkers clinically, including cancer, infectious diseases, neurodegenerative disorders, and cardiovascular diseases. Aptasensors, are electrochemical aptamers based biosensing devices, emerged new class powerful detection technology conventional methods like ELISA Western analysis, primarily because capability for high-performance bioanalysis. review covers current research landscape on exosomes utilizing nanoarchitectonics strategy development aptasensors. Strategies involving signal amplification biofouling prevention discussed, with an emphasis nanoarchitectonics-based bio-interfaces, showcasing enhance sensitivity selectivity through optimal conduction mass transport properties. The ongoing challenges broaden clinical applications these biosensors also highlighted.

Language: Английский

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DNA Nanomaterial-Based Electrochemical Biosensors for Clinical Diagnosis

ACS Nano, Journal Year: 2024, Volume and Issue: 18(46), P. 31713 - 31736

Published: Nov. 7, 2024

Sensitive and quantitative detection of chemical biological molecules for screening, diagnosis monitoring diseases is essential to treatment planning response monitoring. Electrochemical biosensors are fast, sensitive, easy miniaturize, which has led rapid development in clinical diagnosis. Benefiting from their excellent molecular recognition ability high programmability, DNA nanomaterials could overcome the Debye length electrochemical by simple design well suited as elements biosensors. Therefore, enhance sensitivity specificity biosensors, significant progress been made recent years optimizing design. Here, establishment sensing strategies based on reviewed detail. First, structural nanomaterial examined improving overcoming length. In addition, electrical signal transduction amplification reviewed, applications nanomaterial-based integrated devices further summarized. Finally, main opportunities challenges detecting disease biomarkers presented an aim guide with specificity.

Language: Английский

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Single‐Response Duplexing of Electrochemical Label‐Free Biosensor from the Same Tag

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(11)

Published: Jan. 21, 2024

Abstract Multiplexing is a valuable strategy to boost throughput and improve clinical accuracy. Exploiting the vertical, meshed design of reproducible low‐cost ultra‐dense electrochemical chips, unprecedented single‐response multiplexing typical label‐free biosensors reported. Using cheap, handheld one‐channel workstation single redox probe, that is, ferro/ferricyanide, recognition events taking place on two spatially resolved locations same working electrode can be tracked along voltammetry scan by collecting signatures probe in relation different quasi‐reference electrodes, Au (0 V) Ag/AgCl ink (+0.2 V). This spatial isolation prevents crosstalk between tags interferences over functionalization binding steps, representing an advantage existing non‐spatially multiplex strategies. As proof concept, peptide‐tethered immunosensors are demonstrated provide duplex detection COVID‐19 antibodies, thereby doubling while achieving 100% accuracy serum samples. The approach envisioned enable broad applications high‐throughput multi‐analyte platforms, as it tailored other biosensing devices formats.

Language: Английский

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