
Chemical Science, Journal Year: 2024, Volume and Issue: 15(31), P. 12291 - 12300
Published: Jan. 1, 2024
Herein, intensified electrochemiluminescence (ECL) and photoluminescence (PL)
Language: Английский
Chemical Science, Journal Year: 2024, Volume and Issue: 15(31), P. 12291 - 12300
Published: Jan. 1, 2024
Herein, intensified electrochemiluminescence (ECL) and photoluminescence (PL)
Language: Английский
Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(17), P. 7741 - 7749
Published: April 19, 2022
Luminol-based electrochemiluminescence (ECL) can be readily excited by various reactive oxygen species (ROS) electrogenerated with an reduction reaction (ORR). However, the multiple active intermediates involved in ORR catalyzed complex nanomaterials lead to recognizing role of ROS still elusive. Moreover, suffering from absence direct electrochemical oxidation luminol at cathode and poor transformation efficiency O2 ROS, weak cathodic ECL emission is often neglected. Herein, owing tunable coordination environment structure-dependent catalytic feature, single-atom catalysts (SACs) are employed uncover relationship between intrinsic activity behavior. Interestingly, traditionally negligible first boosted (ca. 70-fold) combination via SACs chemical luminol. The exhibits electron-transfer pathway-dependent response adjusting surrounding center metal atoms a controlled way selectively produce different intermediates. This work bridges performance behavior, which will guide development amplified sensing platform through rational tailoring potential-resolved assays based on high-efficiency reported.
Language: Английский
Citations
155Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(6), P. 3836 - 3843
Published: Feb. 2, 2024
Modulating the electronic structure of metal nanoparticles via metal–support interaction has attracted intense interest in field catalytic science. However, roles supporting substrates regulating properties electrochemiluminescence (ECL) remain elusive. Here, we find that use graphdiyne (GDY) as substrate for electroless deposition Pd (Pd/GDY) produces most pronounced anodic signal enhancement luminol–dissolved oxygen (O2) ECL system co-reactant accelerator over other carbon-based composite nanomaterials. Pd/GDY exhibits electrocatalytic activity reduction O2 through a four-electron pathway at approximately −0.059 V (vs Ag/AgCl) neutral solution forming reactive species (ROS) intermediates. The study shows and GDY increases amount stability ROS on electrode surface promotes reaction luminol anion radical to generate excited luminol, which significantly boosts emission. Based quenching consumption by antioxidants, develop platform detection intracellular antioxidants. This provides an avenue development efficient systems media expands biological application systems.
Language: Английский
Citations
39Biosensors, Journal Year: 2025, Volume and Issue: 15(1), P. 63 - 63
Published: Jan. 19, 2025
The sensitive detection of inflammatory biomarkers in gingival crevicular fluid (GCF) is highly desirable for the evaluation periodontal disease. Luminol-based electrochemiluminescence (ECL) immunosensors offer a promising approach fast and convenient biomarkers. However, luminol’s low ECL efficiency under neutral conditions remains challenge. This study developed an immunosensor by engineering immunorecognition interface on outer surface mesoporous silica nanochannel film (SNF) confining Co3O4 nanocatalyst within SNF nanochannels to improve luminol efficiency. was grown indium tin oxide (ITO) electrode using simple Stöber solution growth method. A successfully confined through situ electrodeposition, confirmed X-ray photoelectron spectroscopy (XPS) electrochemical measurements. demonstrated excellent electrocatalytic activity, effectively enhancing H2O2 oxidation boosting signal conditions. Using interleukin-6 (IL-6) as proof-of-concept demonstration, epoxy functionalization enabled covalent immobilization capture antibodies, forming specific interface. IL-6 binding induced immunocomplex formation, which reduced allowed quantitative detection. showed linear range from 1 fg mL−1 10 ng mL−1, with limit (LOD) 0.64 mL−1. It also good selectivity anti-interference capabilities, enabling successful artificial GCF samples.
Language: Английский
Citations
6Talanta, Journal Year: 2022, Volume and Issue: 248, P. 123611 - 123611
Published: May 29, 2022
Language: Английский
Citations
40Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(10), P. 4795 - 4802
Published: March 3, 2023
Simultaneous detection of different disease markers is significant for clinical diagnosis. In this work, a dual-signal electrochemiluminescence (ECL) immunosensor was constructed the simultaneous carbohydrate antigen 125 (CA125) and human epithelial protein 4 (HE4) ovarian cancer. The results showed that Eu metal–organic framework-loaded isoluminol–Au nanoparticles (Eu MOF@Isolu–Au NPs) could generate strong anodic ECL signal through synergistic interaction; as cathodic luminophore, composite carboxyl-functionalized CdS quantum dots N-doped porous carbon-anchored Cu single-atom catalyst catalyze H2O2 co-reactant to produce large amount •OH O2•–, making signals significantly increase become stable. Based on enhancement strategy, sandwich cancer-associated CA125 HE4 by combining antigen–antibody specific recognition magnetic separation technique. resulting displayed high sensitivity, wide linear response range 0.005∼500 ng mL–1, low limits 0.37 1.58 pg mL–1 HE4, respectively. Furthermore, it had excellent selectivity, stability, practicability in real serum samples. This work establishes framework in-depth design application catalysis sensing.
Language: Английский
Citations
37Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(8), P. 4131 - 4137
Published: Feb. 17, 2023
A novel ultrasensitive electrochemiluminescence (ECL) biosensor was constructed using two-dimensional (2D) Co3O4 nanosheets as a coreaction accelerator of the luminol/H2O2 ECL system for detection microRNA-21 (miRNA-21). Impressively, 2D with effective mutual conversion Co2+/Co3+ redox pair and abundant active sites could promote decomposition coreactant H2O2 to generate more superoxide anion radicals (O2•-), which reacted luminol significantly enhancing signals. Furthermore, trace target miRNA-21 transformed into large number G-wires through strand displacement amplification (SDA) process self-assemble highly ordered rolling DNA nanomachine (HORDNM), tremendously improve sensitivity biosensors. Hence, on basis luminol/H2O2/2D nanosheet ternary system, implemented limit low 4.1 aM, provided strategy design an emitter biomarkers early disease diagnosis.
Language: Английский
Citations
27Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(5), P. 2165 - 2172
Published: Jan. 29, 2024
The profiling of multiple glycans on a single cell is important for elucidating glycosylation mechanisms and accurately identifying disease states. Herein, we developed closed bipolar electrode (BPE) array chip live single-cell trapping in situ galactose sialic acid detection with the electrochemiluminescence (ECL) method. Methylene blue-DNA (MB-DNA) as well biotin-DNA (Bio-DNA) codecorated AuNPs were prepared nanoprobes, which selectively labeled surface through chemoselective labeling techniques. individual was captured microtrap cathodic chamber, under an appropriate potential, MB molecules cellular membrane underwent oxidation, triggering reduction [Ru(bpy)3]2+/TPA consequently generating ECL signals anodic chamber. abundance groups enabled selective monitoring both galactosyl high sensitivity using ECL. content per HepG2 detected to be 0.66 0.82 fmol, respectively. Through comprehensive evaluation these two types cell, tumor cells, normal cells could effectively discriminated accuracy heterogeneous analysis improved. Additionally, dynamic variations also achieved. This work introduced straightforward convenient approach heterogeneity among cells.
Language: Английский
Citations
13Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(16)
Published: Feb. 20, 2024
Abstract Single‐atom catalysts (SACs), distinguished by their maximum atom efficiency and precise control over the coordination electronic properties of individual atoms, show great promise in electrocatalysis. Gaining a comprehensive understanding electrochemical performance SACs requires screening electron transfer process at micro/nano scale. This research pioneers use electrogenerated chemiluminescence microscopy (ECLM) to observe electrocatalytic reactions SACs. It boasts sensitivity single photon level temporal resolution down 100 ms, enabling real‐time capture behavior during potential sweeping. Leveraging direct correlation between ECL emission heterogeneous processes, we introduced flux density for quantitative analysis, unveiling approach systematically reveals relationship based on different metal atoms peroxidase (POD)‐like activity. The outcomes contribute fundamental pave way designing with diverse technological industrial applications.
Language: Английский
Citations
12Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 261, P. 116495 - 116495
Published: June 11, 2024
Language: Английский
Citations
12Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 261, P. 116476 - 116476
Published: June 6, 2024
Language: Английский
Citations
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