Journal of the American Chemical Society,
Journal Year:
2022,
Volume and Issue:
144(17), P. 7741 - 7749
Published: April 19, 2022
Luminol-based
electrochemiluminescence
(ECL)
can
be
readily
excited
by
various
reactive
oxygen
species
(ROS)
electrogenerated
with
an
reduction
reaction
(ORR).
However,
the
multiple
active
intermediates
involved
in
ORR
catalyzed
complex
nanomaterials
lead
to
recognizing
role
of
ROS
still
elusive.
Moreover,
suffering
from
absence
direct
electrochemical
oxidation
luminol
at
cathode
and
poor
transformation
efficiency
O2
ROS,
weak
cathodic
ECL
emission
is
often
neglected.
Herein,
owing
tunable
coordination
environment
structure-dependent
catalytic
feature,
single-atom
catalysts
(SACs)
are
employed
uncover
relationship
between
intrinsic
activity
behavior.
Interestingly,
traditionally
negligible
first
boosted
(ca.
70-fold)
combination
via
SACs
chemical
luminol.
The
exhibits
electron-transfer
pathway-dependent
response
adjusting
surrounding
center
metal
atoms
a
controlled
way
selectively
produce
different
intermediates.
This
work
bridges
performance
behavior,
which
will
guide
development
amplified
sensing
platform
through
rational
tailoring
potential-resolved
assays
based
on
high-efficiency
reported.
Journal of the American Chemical Society,
Journal Year:
2024,
Volume and Issue:
146(6), P. 3836 - 3843
Published: Feb. 2, 2024
Modulating
the
electronic
structure
of
metal
nanoparticles
via
metal–support
interaction
has
attracted
intense
interest
in
field
catalytic
science.
However,
roles
supporting
substrates
regulating
properties
electrochemiluminescence
(ECL)
remain
elusive.
Here,
we
find
that
use
graphdiyne
(GDY)
as
substrate
for
electroless
deposition
Pd
(Pd/GDY)
produces
most
pronounced
anodic
signal
enhancement
luminol–dissolved
oxygen
(O2)
ECL
system
co-reactant
accelerator
over
other
carbon-based
composite
nanomaterials.
Pd/GDY
exhibits
electrocatalytic
activity
reduction
O2
through
a
four-electron
pathway
at
approximately
−0.059
V
(vs
Ag/AgCl)
neutral
solution
forming
reactive
species
(ROS)
intermediates.
The
study
shows
and
GDY
increases
amount
stability
ROS
on
electrode
surface
promotes
reaction
luminol
anion
radical
to
generate
excited
luminol,
which
significantly
boosts
emission.
Based
quenching
consumption
by
antioxidants,
develop
platform
detection
intracellular
antioxidants.
This
provides
an
avenue
development
efficient
systems
media
expands
biological
application
systems.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(1), P. 63 - 63
Published: Jan. 19, 2025
The
sensitive
detection
of
inflammatory
biomarkers
in
gingival
crevicular
fluid
(GCF)
is
highly
desirable
for
the
evaluation
periodontal
disease.
Luminol-based
electrochemiluminescence
(ECL)
immunosensors
offer
a
promising
approach
fast
and
convenient
biomarkers.
However,
luminol’s
low
ECL
efficiency
under
neutral
conditions
remains
challenge.
This
study
developed
an
immunosensor
by
engineering
immunorecognition
interface
on
outer
surface
mesoporous
silica
nanochannel
film
(SNF)
confining
Co3O4
nanocatalyst
within
SNF
nanochannels
to
improve
luminol
efficiency.
was
grown
indium
tin
oxide
(ITO)
electrode
using
simple
Stöber
solution
growth
method.
A
successfully
confined
through
situ
electrodeposition,
confirmed
X-ray
photoelectron
spectroscopy
(XPS)
electrochemical
measurements.
demonstrated
excellent
electrocatalytic
activity,
effectively
enhancing
H2O2
oxidation
boosting
signal
conditions.
Using
interleukin-6
(IL-6)
as
proof-of-concept
demonstration,
epoxy
functionalization
enabled
covalent
immobilization
capture
antibodies,
forming
specific
interface.
IL-6
binding
induced
immunocomplex
formation,
which
reduced
allowed
quantitative
detection.
showed
linear
range
from
1
fg
mL−1
10
ng
mL−1,
with
limit
(LOD)
0.64
mL−1.
It
also
good
selectivity
anti-interference
capabilities,
enabling
successful
artificial
GCF
samples.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(10), P. 4795 - 4802
Published: March 3, 2023
Simultaneous
detection
of
different
disease
markers
is
significant
for
clinical
diagnosis.
In
this
work,
a
dual-signal
electrochemiluminescence
(ECL)
immunosensor
was
constructed
the
simultaneous
carbohydrate
antigen
125
(CA125)
and
human
epithelial
protein
4
(HE4)
ovarian
cancer.
The
results
showed
that
Eu
metal–organic
framework-loaded
isoluminol–Au
nanoparticles
(Eu
MOF@Isolu–Au
NPs)
could
generate
strong
anodic
ECL
signal
through
synergistic
interaction;
as
cathodic
luminophore,
composite
carboxyl-functionalized
CdS
quantum
dots
N-doped
porous
carbon-anchored
Cu
single-atom
catalyst
catalyze
H2O2
co-reactant
to
produce
large
amount
•OH
O2•–,
making
signals
significantly
increase
become
stable.
Based
on
enhancement
strategy,
sandwich
cancer-associated
CA125
HE4
by
combining
antigen–antibody
specific
recognition
magnetic
separation
technique.
resulting
displayed
high
sensitivity,
wide
linear
response
range
0.005∼500
ng
mL–1,
low
limits
0.37
1.58
pg
mL–1
HE4,
respectively.
Furthermore,
it
had
excellent
selectivity,
stability,
practicability
in
real
serum
samples.
This
work
establishes
framework
in-depth
design
application
catalysis
sensing.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(8), P. 4131 - 4137
Published: Feb. 17, 2023
A
novel
ultrasensitive
electrochemiluminescence
(ECL)
biosensor
was
constructed
using
two-dimensional
(2D)
Co3O4
nanosheets
as
a
coreaction
accelerator
of
the
luminol/H2O2
ECL
system
for
detection
microRNA-21
(miRNA-21).
Impressively,
2D
with
effective
mutual
conversion
Co2+/Co3+
redox
pair
and
abundant
active
sites
could
promote
decomposition
coreactant
H2O2
to
generate
more
superoxide
anion
radicals
(O2•-),
which
reacted
luminol
significantly
enhancing
signals.
Furthermore,
trace
target
miRNA-21
transformed
into
large
number
G-wires
through
strand
displacement
amplification
(SDA)
process
self-assemble
highly
ordered
rolling
DNA
nanomachine
(HORDNM),
tremendously
improve
sensitivity
biosensors.
Hence,
on
basis
luminol/H2O2/2D
nanosheet
ternary
system,
implemented
limit
low
4.1
aM,
provided
strategy
design
an
emitter
biomarkers
early
disease
diagnosis.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(5), P. 2165 - 2172
Published: Jan. 29, 2024
The
profiling
of
multiple
glycans
on
a
single
cell
is
important
for
elucidating
glycosylation
mechanisms
and
accurately
identifying
disease
states.
Herein,
we
developed
closed
bipolar
electrode
(BPE)
array
chip
live
single-cell
trapping
in
situ
galactose
sialic
acid
detection
with
the
electrochemiluminescence
(ECL)
method.
Methylene
blue-DNA
(MB-DNA)
as
well
biotin-DNA
(Bio-DNA)
codecorated
AuNPs
were
prepared
nanoprobes,
which
selectively
labeled
surface
through
chemoselective
labeling
techniques.
individual
was
captured
microtrap
cathodic
chamber,
under
an
appropriate
potential,
MB
molecules
cellular
membrane
underwent
oxidation,
triggering
reduction
[Ru(bpy)3]2+/TPA
consequently
generating
ECL
signals
anodic
chamber.
abundance
groups
enabled
selective
monitoring
both
galactosyl
high
sensitivity
using
ECL.
content
per
HepG2
detected
to
be
0.66
0.82
fmol,
respectively.
Through
comprehensive
evaluation
these
two
types
cell,
tumor
cells,
normal
cells
could
effectively
discriminated
accuracy
heterogeneous
analysis
improved.
Additionally,
dynamic
variations
also
achieved.
This
work
introduced
straightforward
convenient
approach
heterogeneity
among
cells.
Angewandte Chemie International Edition,
Journal Year:
2024,
Volume and Issue:
63(16)
Published: Feb. 20, 2024
Abstract
Single‐atom
catalysts
(SACs),
distinguished
by
their
maximum
atom
efficiency
and
precise
control
over
the
coordination
electronic
properties
of
individual
atoms,
show
great
promise
in
electrocatalysis.
Gaining
a
comprehensive
understanding
electrochemical
performance
SACs
requires
screening
electron
transfer
process
at
micro/nano
scale.
This
research
pioneers
use
electrogenerated
chemiluminescence
microscopy
(ECLM)
to
observe
electrocatalytic
reactions
SACs.
It
boasts
sensitivity
single
photon
level
temporal
resolution
down
100
ms,
enabling
real‐time
capture
behavior
during
potential
sweeping.
Leveraging
direct
correlation
between
ECL
emission
heterogeneous
processes,
we
introduced
flux
density
for
quantitative
analysis,
unveiling
approach
systematically
reveals
relationship
based
on
different
metal
atoms
peroxidase
(POD)‐like
activity.
The
outcomes
contribute
fundamental
pave
way
designing
with
diverse
technological
industrial
applications.
