Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(40), P. 15008 - 15016
Published: Sept. 26, 2023
Efficient
tuning
of
the
polarity
photoactive
nanomaterials
is
great
importance
in
improving
performance
photoelectrochemical
(PEC)
sensing
platforms.
Herein,
Ag2S/AgInS2
heterojunction
converted
by
radical-induced
positive
feedback
polydopamine
(PDA)
adhesion,
which
further
employed
to
develop
a
signal-switchable
PEC
biosensor.
In
nanocomposites,
Ag2S
and
AgInS2
achieve
electron-hole
separation,
exhibiting
strong
anodic
response.
Under
irradiation
light,
able
produce
superoxide
radical
hydroxyl
intermediate
species,
leading
polymerization
dopamine
(DA)
subsequent
adhesion
PDA
onto
(Ag2S/AgInS2@PDA).
By
constructing
new
electron-transfer
pathway
with
PDA,
converted,
response
changes
from
cathodic
photocurrents.
addition,
since
photoreduction
activity
stronger
than
that
heterojunction,
more
can
be
produced
Ag2S/AgInS2@PDA
once
generated,
thereby
promoting
generation
PDA.
Consequently,
mechanism
established
enhance
conversion
amplify
responding
DA.
As
result,
bioanalytical
method
capable
sensitively
quantifying
DA
10
orders
magnitude
an
ultralow
limit
detection.
Moreover,
applicability
this
biosensor
real
samples
identified
measuring
fetal
bovine
serum
compared
commercial
ELISA
method.
Overall,
work
offers
alternative
perspective
for
adjusting
photogenerated
carriers
designing
high-performance
biosensors.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(11), P. 4589 - 4596
Published: March 5, 2024
Herein,
novel
europium
metal–organic
gels
(Eu-MOGs)
with
excellent
cathode
electrochemiluminescence
(ECL)
emission
are
first
used
to
construct
biosensors
for
the
ultrasensitive
detection
of
miRNA-222.
Impressively,
N
and
O
elements
organic
ligand
2,2′:6,2″-terpyridine
4,4′,4″-tricarboxylic
acid
(H3-tctpy)
can
perfectly
coordinate
Eu3+
form
Eu-MOGs,
which
not
only
reduce
nonradiative
transition
caused
by
intramolecular
free
rotation
phenyl
rings
in
other
MOGs
enhance
ECL
signal
extraordinary
efficiency
as
high
37.2%
(vs
[Ru(bpy)3]2+/S2O82–
system)
but
also
reinforce
ligand-to-metal
charge
transfer
(LMCT)
strong
affinity
between
greatly
improve
stability
signals.
Besides,
an
improved
nucleic
cascade
amplification
reaction
is
developed
raise
conversion
from
target
miRNA-222
a
DNAzyme-mediated
dual-drive
DNA
walker
output
DNA,
simultaneously
shear
specific
recognition
sites
two
directions.
In
that
way,
proposed
biosensor
further
sensitivity
linear
range
10
aM–1
nM
limit
(LOD)
8.5
aM,
achieve
accurate
response
cancer
cell
lysates
MHCC-97L
HeLa.
Additionally,
be
self-regenerated
folding/unfolding
related
triplets
pH
changes
simplify
experimental
operations
cost.
Hence,
this
work
stable
intense
signals
construction
renewable
biosensor,
supplying
reliable
method
biomarker
analysis
disease
diagnosis.
ACS Sensors,
Journal Year:
2023,
Volume and Issue:
8(7), P. 2656 - 2663
Published: June 26, 2023
Currently,
studies
on
electrochemiluminescence
(ECL)
mainly
focused
the
single
emission
of
luminophores
while
those
multi-color
ECL
were
rarely
reported.
Here,
a
bi-mesoporous
composite
metal-organic
framework
(MOF)/covalent-organic
(COF)
with
strong
and
stable
dual-color
was
prepared
to
construct
novel
sensor
for
sensitive
detecting
targets.
A
PTCA-COF
excellent
performance
loaded
great
amount
another
emitter
Cu3(HHTP)2.
Remarkably,
integrated
had
both
properties
at
520
nm
Cu3(HHTP)2
600
wavelengths.
Furthermore,
good
electron
transfer
ability
can
greatly
enhance
electrical
conductivity
promote
electrochemical
activation.
Thus,
simultaneous
enhanced
two-color
intensity
catalytic
conductive
MOF
exerted
dual
enhancement
effect
signal
composite.
Significantly,
diclazepam
not
only
be
adsorbed
well
multi-stage
porous
structure
MOF/COF
by
π-π
interactions
but
also
selectively
quench
PTCA-COF,
realizing
detection.
The
showed
wide
detection
range
from
1.0
×
10-13
10-8
g/L,
limit
(LOD)
as
low
2.6
10-14
g/L
(S/N
=
3).
proposed
preparation
method
simple
sensitive,
providing
new
perspective
potential
application
in
sensing
field.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(27), P. 10457 - 10463
Published: June 29, 2023
The
lumiol-O2
electrochemiluminescence
(ECL)
system
constantly
emits
bright
light
at
positive
potential.
Notably,
compared
with
the
anodic
ECL
signal
of
luminol-O2
system,
great
virtues
cathodic
are
that
it
is
simple
and
causes
minor
damage
to
biological
samples.
Unfortunately,
little
emphasis
has
been
paid
ECL,
owing
low
reaction
efficacy
between
luminol
reactive
oxygen
species.
state-of-the-art
work
mainly
focuses
on
improving
catalytic
activity
reduction
reaction,
which
remains
a
significant
challenge.
In
this
work,
synergistic
amplification
pathway
established
for
ECL.
effect
based
decomposition
H2O2
by
catalase-like
(CAT-like)
CoO
nanorods
(CoO
NRs)
regeneration
carbonate/bicarbonate
buffer.
Compared
Fe2O3
nanorod
(Fe2O3
NR)-
NiO
microsphere-modified
glassy
carbon
electrodes
(GCEs),
intensity
nearly
50
times
stronger
when
potential
ranged
from
0
-0.4
V
NR-modified
GCE
in
carbonate
buffer
solution.
CAT-like
NRs
decompose
electroreduction
product
into
OH·
O2·-,
further
oxidize
HCO3-
CO32-
HCO3·
CO3·-.
These
radicals
very
effectively
interact
form
radical.
More
importantly,
can
be
regenerated
dimerizes
produce
(CO2)2*,
provides
cyclic
during
dimerization
HCO3·.
This
inspires
developing
new
avenue
improve
deeply
understand
mechanism
reaction.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(37), P. 14143 - 14149
Published: Sept. 7, 2023
High-performance
electrochemiluminescence
is
a
significant
approach
for
the
examination
of
disease
biomarkers,
and
utilization
innovative
detection
systems
represents
viable
strategy
to
enhance
efficacy
ECL
analysis.
In
this
work,
biomimetic
engineering
metal-organic
framework
(MOF-818)
has
realized
ultrasensitive
immunoassay
markers
based
on
guidance
free
radical
scavenging
provided
by
antioxidant
cascade.
Initially,
we
synthesized
hydrogen-bonded
organic
(HOF)
consisting
luminol
three
active
ligands
simple
room-temperature
self-assembly.
The
luminol-HOF
(L-HOF)
showed
more
stable
brighter
luminescence
activity
than
monomer
due
nano-confinement
enhancement
coordinated
units.
Subsequently,
MOF-818
with
superoxide
dismutase
(SOD)
catalase
(CAT)
activities
were
recruited
first
time
as
quenching
agents
sandwich
mode.
enzyme
leads
reverse
transformation
anion
radicals
(O2-)
further
decomposition,
decreasing
in
responsiveness
signals.
Using
carcinoembryonic
antigen
(CEA)
an
analytical
model,
limit
0.457
pg/mL
was
obtained
within
range
0.001-50
ng/mL.
We
believe
that
novel
sensing
model
provides
meaningful
potential
tool
precise
detection,
expanding
broader
application
nanoenzymes
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(34), P. 12754 - 12760
Published: Aug. 17, 2023
In
this
study,
a
pH-stimulated
self-locked
DNA
nanostructure
(SLDN)
was
developed
to
efficiently
distinguish
cancer
cells
from
other
for
the
simultaneous
detection
and
imaging
of
endogenous
dual-microRNAs
(miRNAs).
Impressively,
SLDN
specifically
unlocked
in
acidic
environment
form
unlocked-SLDN
disengage
i-motif
sequence
with
labeled
fluorophore
recovery
fluorescence
signal,
resulting
differentiation
normal
cells.
Meanwhile,
could
combine
recognize
targets
miRNA-21
miRNA-155
simultaneously
trigger
hybridization
chain
reaction
(HCR)
amplification
sensitive
dual-miRNA
detection,
limits
1.46
pM
0.72
miRNA-155.
Significantly,
compared
current
miRNA
strategy
based
on
traditional
nanostructure,
proposed
here
remarkably
eliminates
interference
achieve
high-resolution
colocation
miRNAs
tumor
an
ultralow
background
signal.
This
work
provided
specific
method
materialize
biomarker
distinguishable
high-definition
live-cell
precise
diagnosis
multifactor
research
progression.
