A Programmable Automatic Cascade Machinery for Single-Molecule Profiling of Multiple Noncoding RNAs in Breast Tissues DOI
Li Wen, Wenjing Liu, Jun Lu

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

Noncoding RNAs (ncRNAs) are identified as critical regulatory molecules in tumorigenesis and progression. Investigating the expression patterns of multiple ncRNAs living cells tissues may facilitate diagnosis cancers. Herein, we develop a programmable automatic cascade machinery for single-molecule profiling ncRNAs. This method involves two successive amplification events that can convert extremely low-abundance target into abundant FAM/Cy5 generation amplified fluorescence signals. The subsequent detection identify piR-36026 with FAM signal DSCAM-AS1 Cy5 signal. Due to high efficiency signal-to-noise ratio imaging, this achieve sensitive limit 44.67 aM 45.71 DSCAM-AS1, it measure endogenous at single-cell level. Moreover, healthy breast cancer demonstrates feasibility proposed diagnostics. By programming recognition sequences dumbbell probes, be extended other cancer-related ncRNAs, great prospects clinical applications.

Language: Английский

Supramolecular systems for bioapplications: recent research progress in China DOI
Yue‐Yang Liu, Xiao-Yong Yu, Yu‐Chen Pan

et al.

Science China Chemistry, Journal Year: 2024, Volume and Issue: 67(5), P. 1397 - 1441

Published: March 22, 2024

Language: Английский

Citations

30
A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food DOI
Yuan Zhao, Wenqin Wu, Xiaoqian Tang

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 225, P. 115102 - 115102

Published: Jan. 25, 2023

Language: Английский

Citations

28
The Landscape of Biomimetic Nanovesicles in Brain Diseases DOI Open Access
Qing You, Fuming Liang,

Gege Wu

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 36(7)

Published: Sept. 15, 2023

Abstract Brain diseases, such as brain tumors, neurodegenerative cerebrovascular and injuries, are caused by various pathophysiological changes, which pose a serious health threat. disorders often difficult to treat due the presence of blood–brain barrier (BBB). Biomimetic nanovesicles (BNVs), including endogenous extracellular vesicles (EVs) derived from cells artificial nanovesicles, possess ability penetrate BBB thus can be utilized for drug delivery brain. BNVs, especially EVs, widely distributed in body fluids usually carry disease‐related signal molecules proteins, RNA, DNA, may also analyzed understand etiology pathogenesis diseases. This review covers exhaustive classification characterization BNVs roles involved emphatically focuses on nanotechnology‐integrated disease theranostics, diagnosis strategies precise therapeutic regulations (e.g., immunity regulation, disordered protein clearance, anti‐neuroinflammation, neuroregeneration, angiogenesis, gut–brain axis regulation). The remaining challenges future perspectives regarding treatment diseases discussed outlined.

Language: Английский

Citations

28
Microporous PdCuB nanotag-based electrochemical aptasensor with Au@CuCl2 nanowires interface for ultrasensitive detection of PD-L1-positive exosomes in the serum of lung cancer patients DOI Creative Commons

Luyue Chang,

Haiping Wu, Rui Chen

et al.

Journal of Nanobiotechnology, Journal Year: 2023, Volume and Issue: 21(1)

Published: March 11, 2023

Programmed cell death ligand 1 protein-positive (PD-L1+) exosomes have been found to be a potential biomarker for the diagnosis of non-small lung cancer (NSCLC). However, development highly sensitive detection technique PD-L1+ is still challenge in clinical applications. Herein, sandwich electrochemical aptasensor based on ternary metal-metalloid palladium-copper-boron alloy microporous nanospheres (PdCuB MNs) and Au@CuCl2 nanowires (NWs) was designed exosomes. The excellent peroxidase-like catalytic activity PdCuB MNs high conductivity NWs endow fabricated with intense signal, thus enabling low abundance analytical results revealed that maintained favorable linearity over wide concentration range 6 orders magnitude reached limit 36 particles/mL. successfully applied analysis complex serum samples achieves accurate identification NSCLC patients. Overall, developed provides powerful tool early NSCLC.

Language: Английский

Citations

25
One-step multiplex analysis of breast cancer exosomes using an electrochemical strategy assisted by gold nanoparticles DOI

Mingwan Zhang,

Liang Xia, Wenjing Mei

et al.

Analytica Chimica Acta, Journal Year: 2023, Volume and Issue: 1254, P. 341130 - 341130

Published: March 22, 2023

Language: Английский

Citations

25
Proximity-Guaranteed DNA Machine for Accurate Identification of Breast Cancer Extracellular Vesicles DOI
Shuang Yang, Liang Zhou,

Zhikai Fang

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(4), P. 2194 - 2202

Published: April 15, 2024

Breast cancer is one of the most diagnosed cancers worldwide. Precise diagnosis and subtyping have important significance for targeted therapy prognosis prediction breast cancer. Herein, we design a proximity-guaranteed DNA machine accurate identification extracellular vesicles (EVs), which beneficial to explore subtype features In our design, two proximity probes are located close on same EV through specific recognition coexisting surface biomarkers, thus being ligated with help click chemistry. Then, product initiates operation involving catalytic hairpin assembly clusters regularly interspaced short palindromic repeats (CRISPR)-Cas12a-mediated trans-cleavage, finally generates significant response that enables EVs expressing both biomarkers. Principle-of-proof studies performed using derived from cell line BT474 as models, confirming high sensitivity specificity machine. When further applied clinical samples, shown be capable not only distinguishing patients special subtypes but also realizing tumor staging regarding disease progression. Therefore, work may provide new insights into subtype-based well more potential therapeutic targets in future.

Language: Английский

Citations

11
CRISPR/Cas detection with nanodevices: moving deeper into liquid biopsy DOI
Huimin Kong, Ke Yi, Rachel L. Mintz

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(17), P. 2301 - 2319

Published: Jan. 1, 2024

This feature article summarises the latest advances, potential challenges, and future prospects of CRISPR/Cas gene-editing technologies in conjunction with emerging nanodevices for refinement precision liquid biopsy techniques.

Language: Английский

Citations

10
Tunable membrane penetration-directed signal amplification for electrochemical determination of matrix metalloproteinase-2 in cancer diagnosis DOI

Guozhang Zhou,

Yizhang Wu,

Yulin Xiong

et al.

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 431, P. 137410 - 137410

Published: Feb. 7, 2025

Language: Английский

Citations

1
Reliable Detection of Extracellular PD-L1 by DNA Computation-Mediated Microfluidics DOI
Yuqian Zhang, Qiuyue Wu,

Yihao Huang

et al.

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(24), P. 9373 - 9379

Published: June 5, 2023

Extracellular vesicle PD-L1 (programmed death-1 ligand 1) is of greater value in tumor diagnosis, prognosis, and efficacy monitoring anti-PD-1/PD-L1 immunotherapy. However, soluble interferes with the accurate detection extracellular (EV) PD-L1. Here, we developed a microfluidic differentiation method for PD-L1, without interference soluble, by DNA computation lipid probes aptamer as inputs (DECLA). For DECLA method, cholesterol-DNA probe was designed that efficiently embeds into EV membrane, an aptamer-based used recognition. Due to stable secondary structure connector, only cobinding affinity induced biotin-labeled connector activation, while cannot hybridize. As result, EVs can be captured streptavidin-functioned herringbone chip quantified anti-CD63-induced fluorescence signal. The high specificity dual-input allied sensitivity microfluidic-based suitable distinguishing lung cancer patients from healthy donors, highlighting its potential translation clinical diagnosis therapy monitoring.

Language: Английский

Citations

18
Recent Advancement of PD-L1 Detection Technologies and Clinical Applications in the Era of Precision Cancer Therapy DOI Creative Commons
Yuanfeng Zhang,

Juanjuan Wu,

Chaobin Zhao

et al.

Journal of Cancer, Journal Year: 2023, Volume and Issue: 14(5), P. 850 - 873

Published: Jan. 1, 2023

Programmed death-1 is a protein found on the surface of immune cells that can interact with its ligand, programmed death-ligand 1 (PD-L1), which expressed plasma membrane, secreted cellular exosomes, in cell nuclei, or as circulating soluble protein. This interaction lead to escape cancer patients. In clinical settings, PD-L1 plays an important role tumor disease diagnosis, determining therapeutic effectiveness, and predicting patient prognosis. inhibitors are also essential components immunotherapy. Thus, detection levels crucial, especially era precision therapy. recent years, innovations have been made traditional immunoassay methods development new immunoassays for detection. review aims summarize research progress technology highlight applications PD-L1.

Language: Английский

Citations

16