Homogeneous Aptasensor with Electrochemical and Electrochemiluminescence Dual Detection Channels Enabled by Nanochannel-Based Probe Enrichment and DNase I Cleavage for Tumor Biomarker Detection

Molecules, Journal Year: 2025, Volume and Issue: 30(3), P. 746 - 746

Published: Feb. 6, 2025

Homogeneous aptasensors that eliminate the need for probe labeling or immobilization hold significant potential rapid detection of tumor biomarkers. Herein, a homogeneous aptasensor with electrochemical (EC) and electrochemiluminescence (ECL) dual channels was developed by integrating nanochannel-based enrichment DNase I cleavage selective biomarker, carbohydrate antigen 125 (CA125). A two-dimensional (2D) composite prepared assembling CA125-specific aptamer cationic tris(2,2′-bipyridyl)Ru(II) (Ru(bpy)32+), which exhibited both EC ECL properties, onto graphene oxide (GO) nanosheets (Ru(bpy)32+/Apt@GO). vertically ordered mesoporous silica film (VMSF) ultrasmall, uniform, aligned nanochannel arrays rapidly grown on inexpensive disposable indium tin (ITO) electrode, forming interface. Due to size exclusion effect ultrasmall nanochannels in VMSF, Ru(bpy)32+/Apt@GO unable penetrate nanochannels, resulting no detectable Ru(bpy)32+ signal electrode. Upon specific recognition CA125 aptamer, an aptamer-CA125 complex formed subsequently detached from GO. then cleaved complex, releasing allowing dissociate into solution. This enzymatic enabled re-enter binding cycle, amplifying release The electrostatic adsorption VMSF significantly enhanced signals. constructed linear range 0.1 U/mL 100 ng/mL, limit (LOD) 91 mU/mL. For detection, detected over 0.001 U/mL, LOD as low 0.4 demonstrated excellent selectivity successfully applied dual-mode EC/ECL fetal bovine serum samples.

Language: Английский

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Simultaneous Detection of Ovarian Cancer-Concerned HE4 and CA125 Markers Based on Cu Single-Atom-Triggered CdS QDs and Eu MOF@Isoluminol ECL

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(10), P. 4795 - 4802

Published: March 3, 2023

Simultaneous detection of different disease markers is significant for clinical diagnosis. In this work, a dual-signal electrochemiluminescence (ECL) immunosensor was constructed the simultaneous carbohydrate antigen 125 (CA125) and human epithelial protein 4 (HE4) ovarian cancer. The results showed that Eu metal–organic framework-loaded isoluminol–Au nanoparticles (Eu MOF@Isolu–Au NPs) could generate strong anodic ECL signal through synergistic interaction; as cathodic luminophore, composite carboxyl-functionalized CdS quantum dots N-doped porous carbon-anchored Cu single-atom catalyst catalyze H2O2 co-reactant to produce large amount •OH O2•–, making signals significantly increase become stable. Based on enhancement strategy, sandwich cancer-associated CA125 HE4 by combining antigen–antibody specific recognition magnetic separation technique. resulting displayed high sensitivity, wide linear response range 0.005∼500 ng mL–1, low limits 0.37 1.58 pg mL–1 HE4, respectively. Furthermore, it had excellent selectivity, stability, practicability in real serum samples. This work establishes framework in-depth design application catalysis sensing.

Language: Английский

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Dual-channel molecularly imprinted sensor based on dual-potential electrochemiluminescence of Zn-MOFs for double detection of trace chloramphenicol

Food Chemistry, Journal Year: 2023, Volume and Issue: 413, P. 135627 - 135627

Published: Feb. 3, 2023

Language: Английский

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An electrochemiluminescence sensor based on lanthanide bimetallic MOFs with a “cascade sensitization mechanism” for the sensitive detection of CA242

Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125956 - 125956

Published: March 22, 2024

Language: Английский

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Gold Nanocluster Probe-Based Electron-Transfer-Mediated Electrochemiluminescence Sensing Strategy for an Ultrasensitive Copper Ion Detection

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(45), P. 15896 - 15901

Published: Nov. 3, 2022

Exploration of a novel and efficient sensing mechanism Au nanocluster (AuNC)-based electrochemiluminescence (ECL) sensors is still great challenge opportunity for further applications. Herein, we proposed that the electron transfer (ET) could be used as regulation factor construction an ECL-sensing platform based on AuNC probe. As proof-of-concept, ECL quenching effect Cu2+ pre-oxidation-treated l-methionine-capped AuNCs (OM-AuNCs) was investigated in detail. The results revealed after electrochemical excitation probe, transferred from highest occupied molecular orbital (HOMO) Met-Cu2+ to OM-AuNCs, along with ET lowest-unoccupied (LUMO) OM-AuNCs back HOMO Met-Cu2+, leading OM-AuNCs. Since intensity sensitively affected by process, preferable linear dependence obtained concentration range 1.0 × 10–18 10–14 M high selectivity. More importantly, record low detection limit (LOD, 2.3 10–20 M) at single copper ion level has been realized without any other amplification technique. Furthermore, actual sample exhibited satisfactory results. Therefore, this study enriches ET-mediated application promotes more rational design sensors.

Language: Английский

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A novel MCI-ECL dual organic ligand MOFs with low-potential by band gap regulation for ultrasensitive S.aureus detection

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162433 - 162433

Published: April 1, 2025

Language: Английский

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Progress, enhancement mechanisms and applications of electrochemiluminescence and photoluminescence metal nanoclusters

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 170, P. 117443 - 117443

Published: Nov. 18, 2023

Language: Английский

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Energy Level Engineering in Gold Nanoclusters for Exceptionally Bright NIR Electrochemiluminescence at a Low Trigger Potential

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(29), P. 11106 - 11112

Published: July 11, 2023

Electrochemiluminescence (ECL) is a widely used light output mechanism from electrochemical excitation. Understanding the intrinsic essence for ideal ECL generation remains fundamental challenge. Here, based on molecular orbital theory, we reported an energy level engineering strategy to regulate performance by using ligand-protected gold nanoclusters (AuNCs) as luminophores and N,N-diisopropylethylamine (DIPEA) coreactant. The matching between AuNCs DIPEA effectively promoted their electron transfer reactions, thus improving excitation efficiency reducing trigger potential. Simultaneously, narrow band gap of further enabled enhanced emission efficiency. Using theory developed here, dual-enhanced was proposed, β-CD-AuNCs were designed verify this mechanism. β-CD-AuNCs/DIPEA system resulted in highly stable near-infrared with unprecedented (145-fold higher than that classic Ru(bpy)32+/tetra-n-butylammonium perchlorate system) low potential 0.48 V. A visual NIR-ECL successfully realized infrared camera. This work provides original mechanistic understanding designing efficient systems, which promises be harbinger broad applicability other systems sensing platforms.

Language: Английский

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Protein-Directed Au(0)-Rich Gold Nanoclusters as Ratiometric Luminescence Sensors for Auric Ions via Comproportionation-Induced Emission Enhancement

Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(14), P. 5886 - 5893

Published: March 27, 2023

Gold nanoclusters (Au NCs) are widely used as fluorescent probes in biomedical sensing and imaging due to their versatile optical properties low cytotoxicity. Surface engineering of gold aims design a surface with physicochemical performances, but previous investigations have primarily focused on the acquisition "brightest" species. This has resulted other types Au NC being neglected. In present study, our group prepared series NCs that were rich Au(0), using "aged" form bovine serum albumin (BSA) via controlling pH during synthesis. We found slight increases alkalinity synthesis over which produced most intensive photoluminescence generated "darkest" NCs, exhibited strongest absorption. These included more atoms had higher Au(0) content. Furthermore, addition Au3+ quenched emission increased NCs. The Au(I) proportion observed Au3+-treated novel comproportionation-induced enhancement effect, we utilized construct "turn-on" ratiometric sensor for toxic Au3+. simultaneous, opposite effects blue-emissive diTyr BSA residues red-emissive After optimization, successfully constructed sensors high sensitivity, selectivity, accuracy. study will inspire new pathway redesign protein-framed analytical methodology comproportionation chemistry.

Language: Английский

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Surface motifs regulated aggregation induced emission in AuAg nanoclusters combined with Ce(III)/Ce(IV) catalytic cyclic amplification strategy for sensitive bioanalysis

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 400, P. 134911 - 134911

Published: Nov. 3, 2023

Language: Английский

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