Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)
Published: April 29, 2025
Language: Английский
Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 247, P. 115929 - 115929
Published: Dec. 15, 2023
Language: Английский
Citations
32
Cellular & Molecular Biology Letters, Journal Year: 2024, Volume and Issue: 29(1)
Published: March 8, 2024
Abstract Persistent infection with high-risk human papillomavirus (HR-HPV) is the primary and initiating factor for cervical cancer. With over 200 identified HPV types, including 14 types that integrate into host epithelial cell DNA, early determination of type crucial effective risk stratification management. Presently, on-site immediate testing during screening stage, known as Point Care Testing (POCT), remains immature, severely limiting scope scenarios screening. This study, guided by genomic sequence patterns HPV, established a multiplex recombinase polymerase amplification (RPA) technology based on concept “universal primers.” approach achieved multiple RPA, coupled CRISPR/Cas12a system serving medium signal conversion. The study successfully constructed POCT combined detection system, denoted H-MRC12a (HPV—Multiple RPA—CRISPR/Cas12a), applied it to typing detection. accomplished six (16, 18, 31, 33, 35, 45) can be completed within 40 min, entire process, from sample loading result interpretation, 45 depth reaching 1 copy/μL each type. Validation system’s reproducibility specificity was further conducted through QPCR 34 clinical samples. Additionally, this explored optimized RPA CRISPR at molecular mechanism level. Furthermore, primer design strategy developed in offers potential enhance throughput while ensuring sensitivity, providing novel research avenue high-throughput Point-of-Care pathogen studies.
Language: Английский
Citations
16
Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(2)
Published: Jan. 31, 2024
Abstract Persistent infection with high‐risk types of human papillomaviruses (HPV) is a major cause cervical cancer, and an important factor in other malignancies, for example, head neck cancer. Despite recent progress screening vaccination, the incidence mortality are still relatively high, especially low‐income countries. The financial burden associated treatment could be decreased if simple, rapid, inexpensive technology HPV testing becomes available, targeting individuals further monitoring increased risk developing Commercial tests available market often expensive, time‐consuming, require sophisticated instrumentation, which limits their more widespread utilization. To address these challenges, novel technologies being implemented also diagnostics that include isothermal amplification techniques, lateral flow assays, CRISPR‐Cas‐based systems, as well microfluidics, paperfluidics lab‐on‐a‐chip devices, ideal point‐of‐care decentralized settings. In this review, we first evaluate current commercial tests, followed by description advanced technologies, explanation principles, critical evaluation strengths weaknesses, suggestions possible implementation into medical diagnostics.
Language: Английский
Citations
14
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 8091 - 8108
Published: March 7, 2024
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTCRISPR-Powered Strategies for Amplification-Free Diagnostics of Infectious DiseasesYupiao XiaYupiao XiaState Key Laboratory Magnetic Resonance and Atomic Molecular Physics, in Biological Systems, National Center Wuhan, Wuhan Institute Physics Mathematics, Innovation Academy Precision Measurement Science Technology, Chinese Sciences − Optoelectronics, 430071, ChinaUniversity Sciences, Beijing 100049, ChinaMore by Yupiao XiaView Biography, Ruotong RaoRuotong RaoState RaoView Mengqiu XiongMengqiu XiongState ChinaDepartment Medicine, Nanjing First Hospital, Medical University, 210006, XiongView Bangshun He*Bangshun HeDepartment China*[email protected]More HeView Bingxin ZhengBingxin ZhengState ZhengView Yanwei JiaYanwei JiaState-Key Analog Mixed-Signal VLSI, Microelectronics, University Macau, Macau 999078, JiaView Biographyhttps://orcid.org/0000-0001-5795-7450, Ying Li*Ying LiState LiView Biographyhttps://orcid.org/0000-0001-8689-5578, Yunhuang Yang*Yunhuang YangState ChinaOptics Valley Laboratory, Hubei 430074, YangView BiographyCite this: Anal. Chem. 2024, 96, 20, 8091–8108Publication Date (Web):March 7, 2024Publication History Received27 September 2023Accepted23 February 2024Revised7 2024Published online7 March inissue 21 May 2024https://pubs.acs.org/doi/10.1021/acs.analchem.3c04363https://doi.org/10.1021/acs.analchem.3c04363review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views1329Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biotechnology,Fluorescence,Genetics,Nucleic acids,SARS-CoV-2 Get e-Alerts
Language: Английский
Citations
12
Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 29, 2025
The incidence of cervical cancer continues to rise in underdeveloped regions due low human papillomavirus (HPV) vaccination rates and inadequate screening systems. To achieve convenient, rapid, accurate detection HPV, we developed a three-wire lateral flow strip assay system based on dual-OR logic gates for rapid simultaneous HPV subtypes 16 18 single test. combines three-branch-catalytic hairpin assembly (TCHA)-mediated signal amplification with simple OR gate-based output improve while enabling 16/18 subtype identification. limit the method was calculated be 10 aM selected target sequences. Meanwhile, showed excellent specificity no false-positive real-world detection. sensitivity colorimetric test strips exceeded 90%, fluorescence-based surpassed 95% detecting clinical samples, demonstrating high degree concordance results obtained from real-time quantitative polymerase chain reaction (qPCR). This provides easy HPV.
Language: Английский
Citations
1
Talanta, Journal Year: 2025, Volume and Issue: 291, P. 127815 - 127815
Published: Feb. 27, 2025
Language: Английский
Citations
1
Biosensors, Journal Year: 2023, Volume and Issue: 13(4), P. 490 - 490
Published: April 19, 2023
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing disease (COVID-19) outbreak and a rising demand for the development of accurate, timely, cost-effective diagnostic tests SARS-CoV-2 as well other viral infections in general. Currently, traditional virus screening methods such plate culturing real-time PCR are considered gold standard with accurate sensitive results. However, these still require sophisticated equipment, trained personnel, long analysis time. Alternatively, integration microfluidic biosensor technologies, microfluidic-based biosensors offer ability to perform sample preparation simultaneous detection many analyses one platform. High sensitivity, accuracy, portability, low cost, high throughput, can be achieved using single This review presents recent advances from works demonstrate advantages merging two technologies sensing viruses. Different platforms classified into main sections: immunoassays molecular assays. Moreover, available commercial analyzed.
Language: Английский
Citations
21
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: July 25, 2024
Abstract Addressing the global disparity in cancer care necessitates development of rapid and affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical cancer, where molecular detection human papillomavirus (HPV) has emerged as an accurate screening method. However, implementing this transition low- middle-income countries been challenging due to high costs centralized facilities required current NA tests. Here, we present CreDiT (CRISPR Enhanced Digital Testing) on-site detection. The platform integrates i) a one-pot CRISPR strategy that simultaneously amplifies both target NAs analytical signals ii) robust fluorescent based on digital communication (encoding/decoding) technology. These features enable assay (<35 minutes) single streamlined workflow. We demonstrate sensitive cell-derived HPV DNA targets down copies identification types clinical brushing specimens ( n = 121).
Language: Английский
Citations
8
Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14
Published: Feb. 28, 2024
Cervical cancer is the third most common threatening women’s health globally, and high-risk human papillomavirus (HR-HPV) infection main cause of cervical worldwide. Given recurrent nature HR-HPV infection, accurate screening essential for its control. Since commonly used polymerase chain reaction (PCR) technique limited by professional equipment personnel, convenient ultrasensitive detection methods are still highly needed. As new molecular methods, nucleic acid amplification-based biosensors have advantages high sensitivity, rapid operation, portability, which helpful point-of-care testing in rural remote areas. This review summarized based on a variety amplification strategies involved improved PCR, loop-mediated isothermal amplification, recombinase hybridization reaction, catalyzed hairpin assembly, CRISPR/Cas systems. In combination with microfluidic technology, lateral flow assays, electrochemical analysis other sensing technologies, throughput, short response time, sensitivity easy operation field. Although there shortcomings, such as cost poor reproducibility, this approach will be suitable on-site or auxiliary clinical diagnosis complex environments areas future.
Language: Английский
Citations
6
Physics of Fluids, Journal Year: 2024, Volume and Issue: 36(2)
Published: Feb. 1, 2024
From their initial application to the fields of chemistry and biology, microfluidic chips used as micro total analysis systems have developed into new technologies satisfy requirements various societal industries. Microchannels are essential components chips; they play a vital role in connecting inlet outlet well determining flow distribution reagent mixing. Microfluidic research has always been devoted minimizing energy dissipation, fluid resistance, pressure drop realize energy-efficient chips. This study proposes theory for manipulating microchannels based on hydrodynamic metamaterials according spatial transformation theory. In particular, specifically designed construct shifters, splitters, combiners, theoretical numerical simulations performed assess performance. The systematic design metamaterial devices proposed this work establishes framework achieve steady state without inducing unstable disturbances complex-shape microchannels.
Language: Английский
Citations
5