Re-vitalizing wastewater: Nutrient recovery and carbon capture through microbe-algae synergy using omics-biology DOI Creative Commons
Muneer Ahmad Malla, Faiz Ahmad Ansari, Faizal Bux

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 259, P. 119439 - 119439

Published: June 18, 2024

Increasing amounts of wastewater is the most pervasive and challenging environmental problem globally. Conventional treatment methods are costly entail huge energy carbon consumption greenhouse gas emissions. Owing to their unique ability capturing resource recovery, microalgae-microbiome based a potential approach widely used for carbon-neutral treatment. Microalgae-bacteria synergy (i.e., functionally beneficial microbial synthetic communities) performs better enhances carbon-sequestration nutrient recovery from plants. This review presents comprehensive information regarding as sustainable agent discusses synergistic approaches effective removal. Moreover, this discusses, role omics-biology Insilco in unravelling understanding algae-microbe synergism response toward Finally, it various microbiome engineering developing microalgae-bacteria partners sequestration wastewater, summarizes future research perspectives on bioremediation.

Language: Английский

Engineered enzymes for the synthesis of pharmaceuticals and other high-value products DOI
Manfred T. Reetz, Ge Qu, Zhoutong Sun

et al.

Nature Synthesis, Journal Year: 2024, Volume and Issue: 3(1), P. 19 - 32

Published: Jan. 4, 2024

Language: Английский

Citations

69
Automated in vivo enzyme engineering accelerates biocatalyst optimization DOI Creative Commons
Enrico Orsi, Lennart Schada von Borzyskowski, Stephan Noack

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 24, 2024

Achieving cost-competitive bio-based processes requires development of stable and selective biocatalysts. Their realization through in vitro enzyme characterization engineering is mostly low throughput labor-intensive. Therefore, strategies for increasing while diminishing manual labor are gaining momentum, such as vivo screening evolution campaigns. Computational tools like machine learning further support efforts by widening the explorable design space. Here, we propose an integrated solution to challenges whereby ML-guided, automated workflows (including library generation, implementation hypermutation systems, adapted laboratory evolution, growth-coupled selection) could be realized accelerate pipelines towards superior

Language: Английский

Citations

26
Biosynthesis of poly(ester amide)s in engineered Escherichia coli DOI
Tong Un Chae, So Young Choi,

Da‐Hee Ahn

et al.

Nature Chemical Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

2
An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis DOI Creative Commons
Vinh Tran, Somesh Mishra, Sarang S. Bhagwat

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Oct. 3, 2023

Abstract Microbial production of succinic acid (SA) at an industrially relevant scale has been hindered by high downstream processing costs arising from neutral pH fermentation for over three decades. Here, we metabolically engineer the acid-tolerant yeast Issatchenkia orientalis SA production, attaining highest titers in sugar-based media low (pH 3) fed-batch fermentations, i.e. 109.5 g/L minimal medium and 104.6 sugarcane juice medium. We further perform batch using a pilot-scale fermenter (300×) achieve 63.1 SA, which can be directly crystallized with yield 64.0%. Finally, simulate end-to-end low-pH pipeline, techno-economic analysis life cycle assessment indicate our process is financially viable reduce greenhouse gas emissions 34–90% relative to fossil-based processes. expect I. serve as general industrial platform organic acids.

Language: Английский

Citations

33
Synthetically-primed adaptation of Pseudomonas putida to a non-native substrate D-xylose DOI Creative Commons
Pavel Dvořák, Barbora Burýšková, Barbora Popelářová

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 26, 2024

Abstract To broaden the substrate scope of microbial cell factories towards renewable substrates, rational genetic interventions are often combined with adaptive laboratory evolution (ALE). However, comprehensive studies enabling a holistic understanding adaptation processes primed by metabolic engineering remain scarce. The industrial workhorse Pseudomonas putida was engineered to utilize non-native sugar D-xylose, but its assimilation into bacterial biochemical network via exogenous xylose isomerase pathway remained unresolved. Here, we elucidate metabolism and establish foundation for further followed ALE. First, native glycolysis is derepressed deleting local transcriptional regulator gene hexR . We then enhance pentose phosphate implanting transketolase transaldolase two lag-shortened strains allow ALE finetune rewired metabolism. Subsequent multilevel analysis reverse provide detailed insights parallel paths carbon source, highlighting enhanced expression along as key events during process.

Language: Английский

Citations

12
Tumor cell membrane‐based vaccines: A potential boost for cancer immunotherapy DOI Creative Commons
Muyang Yang, Jie Zhou,

Liseng Lu

et al.

Exploration, Journal Year: 2024, Volume and Issue: 4(6)

Published: March 28, 2024

Abstract Because therapeutic cancer vaccines can, in theory, eliminate tumor cells specifically with relatively low toxicity, they have long been considered for application repressing progression. Traditional containing a single or few discrete epitopes failed the clinic, possibly due to challenges epitope selection, target downregulation, cell heterogeneity, microenvironment immunosuppression, lack of vaccine immunogenicity. Whole membrane vaccines, which provide rich source antigens, are emerging as viable alternatives. Autologous and allogenic cellular evaluated clinical treatments. Tumor membranes (TCMs) an intriguing antigen source, membrane‐accessible targets and, at same time, serve integrated carriers adjuvants other agents. This review provides summary properties technologies TCM vaccines. Characteristics, categories, mechanisms, preparation methods discussed, demonstrable additional benefits derived from combining chemotherapy, sonodynamic therapy, phototherapy, oncolytic viruses. Further research chemistry, biomedicine, immunology, bioinformatics address current drawbacks could facilitate adoption

Language: Английский

Citations

12
TRYing to evaluate production costs in microbial biotechnology DOI Creative Commons
Oliver Konzock, Jens Nielsen

Trends in biotechnology, Journal Year: 2024, Volume and Issue: 42(11), P. 1339 - 1347

Published: May 28, 2024

Microbial fermentations offer the opportunity to produce a wide range of chemicals in sustainable fashion, but it is important carefully evaluate production costs. This can be done on basis evaluation titer, rate, and yield (TRY) fermentation process. Here we describe how three TRY metrics impact technoeconomics microbial process, illustrate use these for different processes two commodity chemicals, 1,3-propanediol (PDO) ethanol, as well fine chemical penicillin. On our discussions, provide some recommendations should reported when new are described.

Language: Английский

Citations

12
Engineering bacteria for cancer immunotherapy DOI Creative Commons
Jesse G. Zalatan, Lorenzo Petrini, Roger Geiger

et al.

Current Opinion in Biotechnology, Journal Year: 2024, Volume and Issue: 85, P. 103061 - 103061

Published: Jan. 13, 2024

Bacterial therapeutics have emerged as promising delivery systems to target tumors. These engineered live can be harnessed modulate the tumor microenvironment or deliver and selectively release therapeutic payloads A major challenge is bacteria systemically without causing widespread inflammation, which critical for many tumors that are not accessible direct intratumoral injection. We describe potential strategies address this challenge, along with approaches specific payload biocontainment ensure safety. will pave way development of cost-effective, widely applicable next-generation cancer therapeutics.

Language: Английский

Citations

11
Dual cytoplasmic‐peroxisomal compartmentalization engineering and multiple metabolic engineering strategies for high yield non‐psychoactive cannabinoid in Saccharomyces cerevisiae DOI
Yun‐kun Ding, Yuan Ning,

Di Xin

et al.

Biotechnology Journal, Journal Year: 2024, Volume and Issue: 19(2)

Published: Feb. 1, 2024

Abstract CBG (Cannabigerol), a nonpsychoactive cannabinoid, has garnered attention due to its extensive antimicrobial and anti‐inflammatory properties. However, the natural content of in Cannabis sativa L. is minimal. In this study, we developed an engineered cell factory for production using Saccharomyces cerevisiae . We introduced CBGA biosynthetic pathway into S. employed several strategies enhance production. These included dynamically inhibiting competitive bypass key metabolic pathways regulated by Erg20p. Additionally, implemented dual cytoplasmic‐peroxisomal compartmentalization approach further increase Furthermore, ensured efficient optimizing NADPH acetyl‐CoA pools. Ultimately, our strain achieved titer 138 mg L −1 through fed‐batch fermentation 5 bioreactor, facilitated microwave decarboxylation extraction. findings underscore significant potential yeast factories achieving higher yields cannabinoid

Language: Английский

Citations

11
A pilot oral history of plant synthetic biology DOI Creative Commons
Jaya Joshi, Andrew D. Hanson

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(1), P. 36 - 47

Published: Jan. 2, 2024

Abstract The whole field of synthetic biology (SynBio) is only about 20 years old, and plant SynBio younger still. Nevertheless, within that short time, in general has drawn more scientific, philosophical, government, private-sector interest than anything since the recombinant DNA revolution. Plant SynBio, particular, now drawing relation to plants’ potential help solve planetary problems such as carbon capture storage replacing fossil fuels feedstocks. As so young fast-developing, we felt it was too soon try analyze its history. Instead, set out essence SynBio's origins early development through interviews with 8 field's founders, representing 5 countries 3 continents. We then distilled these founders’ personal recollections reflections into this review, centering narrative on timelines for pivotal events, articles, funding programs, quoting from interviews. have archived interview recordings documented timeline entries. This work provides a resource future historical scholarship.

Language: Английский

Citations

10