Non-conventional yeasts: promising cell factories for organic acid bioproduction

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Reconfiguration of the reductive TCA cycle enables high-level succinic acid production by Yarrowia lipolytica

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 20, 2023

Abstract Succinic acid (SA) is an important C4-dicarboxylic acid. Microbial production of SA at low pH results in purification costs and hence good overall process economics. However, redox imbalances limited biosynthesis from glucose via the reductive tricarboxylic (TCA) cycle yeast. Here, we engineer strictly aerobic yeast Yarrowia lipolytica for efficient without control. Introduction TCA into cytosol a succinate dehydrogenase-disrupted strain causes arrested cell growth. Although adaptive laboratory evolution restores growth, NADH supply restricts production. Reconfiguration pathway mitochondria through coupling oxidative regeneration improved In pilot-scale fermentation, engineered produces 111.9 g/L with yield 0.79 g/g within 62 h. This study paves way industrial biobased SA.

Language: Английский

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Recent advances in bio-based production of top platform chemical, succinic acid: an alternative to conventional chemistry

Biotechnology for Biofuels and Bioproducts, Journal Year: 2024, Volume and Issue: 17(1)

Published: May 29, 2024

Succinic acid (SA) is one of the top platform chemicals with huge applications in diverse sectors. The presence two carboxylic groups on terminal carbon atoms makes SA a highly functional molecule that can be derivatized into wide range products. biological route for production cleaner, greener, and promising technological option potential to sequester potent greenhouse gas, dioxide. recycling renewable biomass (an indirect form CO

Language: Английский

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Bio-Electrocatalytically Regulated Selective Succinic Acid Production by Suppressing Pyruvate Channel Using Glycerol and CO₂

ACS Sustainable Chemistry & Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Language: Английский

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Issatchenkia orientalis as a platform organism for cost-effective production of organic acids

Metabolic Engineering, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Self-Buffering system for Cost-Effective production of lactic acid from glucose and xylose using Acid-Tolerant Issatchenkia orientalis

Bioresource Technology, Journal Year: 2024, Volume and Issue: 399, P. 130641 - 130641

Published: March 27, 2024

This study presents a cost-effective strategy for producing organic acids from glucose and xylose using the acid-tolerant yeast, Issatchenkia orientalis. I. orientalis was engineered to produce lactic acid xylose, resulting strain, SD108XL, successfully converted sorghum hydrolysates into acid. In order enable low-pH fermentation, self-buffering strategy, where generated by SD108XL strain during fermentation served as buffer, developed. As result, SD108 produced 67 g/L of 73 40 simulating sugar composition biomass hydrolysates. Moreover, techno-economic analysis (TEA) underscored efficiency in streamlining downstream process, thereby reducing production costs. These results demonstrate potential platform cellulosic

Language: Английский

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Mitochondrial membrane transporters as attractive targets for the fermentative production of succinic acid from glycerol in Saccharomyces cerevisiae

FEMS Yeast Research, Journal Year: 2024, Volume and Issue: 24

Published: Jan. 1, 2024

Previously, we reported an engineered Saccharomyces cerevisiae CEN.PK113-1A derivative able to produce succinic acid (SA) from glycerol with net CO2 fixation. Apart utilization pathway that generates NADH, the strain was equipped NADH-dependent reductive branch of TCA cycle (rTCA) and a heterologous SA exporter. However, results indicated significant amount carbon still entered CO2-releasing oxidative cycle. The current study aimed tune down flux through by targeting mitochondrial uptake pyruvate cytosolic intermediates rTCA pathway, as well succinate dehydrogenase complex. Thus, tested effects deletions MPC1, MPC3, OAC1, DIC1, SFC1, SDH1 on production. highest improvement achieved combined deletion MPC3 SDH1. respective produced up 45.5 g/L SA, reached maximum yield 0.66 gSA/gglycerol, accumulated lowest amounts byproducts when cultivated in shake-flasks. Based obtained data, consider further reduction import highly attractive. Moreover, approaches presented might also be valuable for improving production sugars (instead glycerol) are source carbon.

Language: Английский

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Progress on production of malic acid and succinic acid by industrially-important engineered microorganisms

Journal of Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Complete scheme for biosuccinic acid production from candy-factory waste with simultaneous fermentation-electrochemical product recovery, and purification

Biochemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 109687 - 109687

Published: Feb. 1, 2025

Language: Английский

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Transcriptional profiling reveals the effect of arginine on Actinobacillus succinogenes growth and fermentation

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)

Published: Feb. 27, 2025

Language: Английский

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