TrAC Trends in Analytical Chemistry, Journal Year: 2021, Volume and Issue: 136, P. 116189 - 116189
Published: Jan. 18, 2021
Language: Английский
Chemical Reviews, Journal Year: 2022, Volume and Issue: 122(10), P. 9497 - 9570
Published: March 31, 2022
In-cell structural biology aims at extracting information about proteins or nucleic acids in their native, cellular environment. This emerging field holds great promise and is already providing new facts outlooks of interest both fundamental applied levels. NMR spectroscopy has important contributions on this stage: It brings a broad variety nuclei the atomic scale, which ensures its versatility uniqueness. Here, we detail methods, knowledge, applications biomedical engineering related to in-cell by NMR. We finally propose brief overview main other techniques (EPR, smFRET, cryo-ET, etc.) draw some advisable developments for In era large-scale screenings deep learning, accurate qualitative experimental evidence are as essential ever understand interior life cells. can generate such it does so scale. review meant deliver comprehensive but accessible information, with advanced technical details reflections nature results, future field.
Language: Английский
Citations
81
Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 480, P. 215020 - 215020
Published: Jan. 25, 2023
Language: Английский
Citations
77
Advanced Science, Journal Year: 2023, Volume and Issue: 10(12)
Published: Feb. 19, 2023
Developing activatable fluorescent probes with superlative fluorescence enhancement factor (F/F0 ) to improve the signal-to-noise (S/N) ratio is still an urgent issue. "AND" molecular logic gates are emerging as a useful tool for enhanced selectivity and accuracy. Here, gate developed super-enhancers designing huge F/F0 S/N ratio. It utilizes lipid-droplets (LDs) controllable background input sets target analyte variable input. The tremendously quenching due double locking, thus extreme of obtained. Importantly, this probe can transfer LDs after response occurs. be directly visualized through spatial location without control group. Accordingly, peroxynitrite (ONOO- (CNP2-B) de novo designed. CNP2-B achieves 2600 reacting ONOO- . Furthermore, from mitochondria lipid droplets being activated. higher obtained than commercial 3'-(p-hydroxyphenyl) fluorescein (HPFin vitro in vivo. Therefore, atherosclerotic plaques at mouse models delineated clearly administration situ gel. Such envisioned execute more imaging tasks.
Language: Английский
Citations
44
Chemical Science, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
A one-pot palladium-catalysed arylation process has been used to expedite the synthesis of biaryl α-amino acids, leading discovery an environment-sensitive fluorescent probe that was monitor activity a serine protease.
Language: Английский
Citations
2
Coordination Chemistry Reviews, Journal Year: 2025, Volume and Issue: 533, P. 216550 - 216550
Published: Feb. 24, 2025
Citations
2
Coordination Chemistry Reviews, Journal Year: 2020, Volume and Issue: 427, P. 213557 - 213557
Published: Sept. 16, 2020
Language: Английский
Citations
137
Chemical Society Reviews, Journal Year: 2020, Volume and Issue: 50(2), P. 1219 - 1250
Published: Dec. 8, 2020
Parkinson's disease (PD), as the second most common neurodegenerative disease, is caused by complex pathological processes and currently remains very difficult to treat. PD brings great distress patients imposes a heavy economic burden on society. The number of growing aging population increases worldwide. Therefore, it crucial develop new tools for aiding early diagnosis treatment PD. significant features involved in include abnormal accumulation α-synuclein, metal ion dyshomeostasis, oxidative stress, mitochondrial dysfunction neurotransmitter deficiencies. In recent years, fluorescent probes have emerged powerful bioimaging tool with potential help understand via detection monitoring features. this review, we comprehensively summarize design working mechanisms along their applications various biomarkers. We also discuss current limitations provide perspectives how these can be overcome better suitable application clinical trials future. hope that review provides valuable information guidance development used clinically contributes efficient drugs
Language: Английский
Citations
133
Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(29), P. 15724 - 15742
Published: May 20, 2020
Abstract As a consequence of their intrinsic advantageous properties, luminogens that show aggregation‐induced emission (AIEgens) have received increasing global interest for wide range applications. Whereas general synthetic methods towards AIEgens largely rely on tedious procedures and limited reaction types, various innovative now emerged as complementary, even alternative, strategies. In this Review, we systematically highlight advancements made in metal‐catalyzed functionalization metal‐free‐promoted pathways the construction over past five years, briefly illustrate new perspectives area. The development will enable facile synthesis with great structural diversity multifunctional
Language: Английский
Citations
106
Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(33), P. 17797 - 17809
Published: March 12, 2021
In recent years, polymeric nanoparticles (NPs) have become increasingly popular for in vitro and vivo bioimaging because of their excellent versatility exceptional optical properties. Following a brief introduction on fluorescence photoacoustic imaging, as well the merits using NPs over other types fluorescent probes, this Minireview gives concise summary key advances made years (2017-2020) use imaging. A particular focus is placed various strategies used enhanced applications, including that encapsulate near-infrared dyes, aggregation-induced-emission fluorogens, cationic dyes doped with bulky hydrophobic counterions, semiconducting polymers. Next, current limitations some these NP systems are summarized potential solutions offered to overcome them. Finally, critical considerations regard design given future sensing applications.
Language: Английский
Citations
87
Analytical Chemistry, Journal Year: 2021, Volume and Issue: 93(18), P. 7079 - 7085
Published: April 28, 2021
Pesticide residues, significantly hampering the overall environmental and human health, have become an increasingly severe issue. Thus, developing rapid, cost-effective, sensitive tools for monitoring pesticide residues in food water is extremely important. Compared to conventional chromatographic techniques, enzyme inhibition-based biosensors conjugated with fluorogenic probes provide effective alternative methods detecting due inherent advantages including high selectivity sensitivity, simple operation, capability of providing situ real-time information. However, detection efficiency a single enzyme-targeted biosensor practical samples strongly impeded by structural diversity pesticides their distinct targets. In this work, we developed strategy multienzyme-targeted fluorescent probe design accordingly obtained novel (named as 3CP) presence wide variety pesticides. The designed 3CP, targeting cholinesterases, carboxylesterases, chymotrypsin simultaneously, yielded intense fluorescence solid state upon enzyme-catalyzed hydrolysis. It showed excellent sensitivity against organophosphorus carbamate pesticides, limit dichlorvos achieved 1.14 pg/L. Moreover, it allowed diffusion-resistant visualization live cells zebrafish measurement fresh vegetables, demonstrating promising potential tracking environment biological systems.
Language: Английский
Citations
84