3D extrusion bioprinting

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: Nov. 11, 2021

Language: Английский

---

Emergence of FRESH 3D printing as a platform for advanced tissue biofabrication

APL Bioengineering, Journal Year: 2021, Volume and Issue: 5(1)

Published: Feb. 16, 2021

In tissue engineering, an unresolved challenge is how to build complex 3D scaffolds in order recreate the structure and function of human tissues organs. Additive manufacturing techniques, such as bioprinting, have potential biological material with unprecedented spatial control; however, printing soft materials air often results poor fidelity. Freeform Reversible Embedding Suspended Hydrogels (FRESH) embedded approach that solves this problem by extruding bioinks within a yield-stress support bath holds place until cured. Perspective, we discuss challenges liquid-like emergence for FRESH related techniques solution. This includes development bioprinting field rapid growth adoption, well advantages biofabrication new research has enabled. Specific focus on customizability technique where chemical composition aqueous phase crosslinker can all be tailored wide range structures. Finally, look ahead at future printing, discussing both opportunities see develops.

Language: Английский

---

Natural Hydrogel-Based Bio-Inks for 3D Bioprinting in Tissue Engineering: A Review

Gels, Journal Year: 2022, Volume and Issue: 8(3), P. 179 - 179

Published: March 14, 2022

Three-dimensional (3D) printing is well acknowledged to constitute an important technology in tissue engineering, largely due the increasing global demand for organ replacement and regeneration. In 3D bioprinting, which a step ahead of biomaterial printing, ink employed impregnated with cells, without compromising printability. This allows immediate scaffold cellularization generation complex structures. The use cell-laden inks or bio-inks provides opportunity enhanced cell differentiation fabrication Recognizing importance such bio-inks, current study comprehensively explores state art utilization based on natural polymers (biopolymers), as cellulose, agarose, alginate, decellularized matrix, bioprinting. Discussions regarding progress techniques approaches bioprinting polymers, limitations prospects concerning future trends human-scale are also presented.

Language: Английский

---

Advances in hydrogel-based vascularized tissues for tissue repair and drug screening

Bioactive Materials, Journal Year: 2021, Volume and Issue: 9, P. 198 - 220

Published: July 10, 2021

The construction of biomimetic vasculatures within the artificial tissue models or organs is highly required for conveying nutrients, oxygen, and waste products, improving survival engineered tissues in vitro. In recent times, remarkable progress utilizing hydrogels understanding vascular biology have enabled creation three-dimensional (3D) composed complex systems. this review, we give an emphasis on utilization their advantages vascularization tissues. Initially, significance elements regeneration mechanisms vascularization, including angiogenesis vasculogenesis, are briefly introduced. Further, highlight importance as microenvironments fabricating vascularized organs, terms tunable physical properties, high similarity physiological environments, alternative shaping mechanisms, among others. Furthermore, discuss such hydrogels-based various applications, regeneration, drug screening, organ-on-chips. Finally, put forward key challenges, multifunctionalities hydrogels, selection suitable cell phenotype, sophisticated engineering techniques, clinical translation behind development with towards future development.

Language: Английский

---

Kidney Decellularized Extracellular Matrix Enhanced the Vascularization and Maturation of Human Kidney Organoids

Advanced Science, Journal Year: 2022, Volume and Issue: 9(15)

Published: March 24, 2022

Kidney organoids derived from human pluripotent stem cells (hPSCs) have extensive potential for disease modelling and regenerative medicine. However, the limited vascularization immaturity of kidney been still remained to overcome. Extracellular matrix (ECM) can provide mechanical support a biochemical microenvironment cell growth differentiation. Here in vitro methods using decellularized extracellular (dECM) hydrogel culture hPSC-derived organoids, which vascular network their own endothelial cells, are reported. Single-cell transcriptomics reveal that vascularized cultured dECM more mature patterns glomerular development higher similarity than those without dECM. Differentiation α-galactosidase A (GLA)-knock-out hPSCs generated CRISPR/Cas9 into by method efficiently recapitulate Fabry nephropathy with vasculopathy. Transplantation mouse accelerates recruitment host maintains integrity organized slit diaphragm-like structures The methodology inducing maturation be applied studies development, modeling,

Language: Английский

---

Light‐Activated Decellularized Extracellular Matrix‐Based Bioinks for Volumetric Tissue Analogs at the Centimeter Scale

Advanced Functional Materials, Journal Year: 2021, Volume and Issue: 31(32)

Published: May 13, 2021

Abstract Tissue engineering requires not only tissue‐specific functionality but also a realistic scale. Decellularized extracellular matrix (dECM) is presently applied to the extrusion‐based 3D printing technology. It has demonstrated excellent efficiency as bioscaffolds that allow of living constructs with elaborate microarchitectures well biochemical milieu target tissues and organs. However, dECM bioinks have poor printability physical properties, resulting in limited shape fidelity scalability. In this study, new light‐activated ruthenium/sodium persulfate (dERS) are introduced. The materials can be polymerized via dityrosine‐based cross‐linking system rapid reaction kinetics improved mechanical properties. Complicated high aspect ratios fabricated similar geometry desired increased versatility using dERS. Furthermore, tissue safely regenerative capacity identical pure dECM. dERS may serve platform for wider biofabrication window through building complex centimeter‐scale supporting performances encapsulated cells. This capability opens avenues upscaling production hydrogel‐based without additional processes, applicable medicine.

Language: Английский

---

Application of 3D bioprinting in the prevention and the therapy for human diseases

Signal Transduction and Targeted Therapy, Journal Year: 2021, Volume and Issue: 6(1)

Published: May 14, 2021

Abstract Rapid development of vaccines and therapeutics is necessary to tackle the emergence new pathogens infectious diseases. To speed up drug discovery process, conventional pipeline can be retooled by introducing advanced in vitro models as alternatives disease employing technology for production medicine cell/drug delivery systems. In this regard, layer-by-layer construction with a 3D bioprinting system or other technologies provides beneficial method developing highly biomimetic reliable research. addition, high flexibility versatility offer advantages effective vaccines, therapeutics, relevant Herein, we discuss potential control We also suggest that research could significant platform rapid automated tissue/organ medicines near future.

Language: Английский

---

Application of decellularized bone matrix as a bioscaffold in bone tissue engineering

Journal of Biological Engineering, Journal Year: 2022, Volume and Issue: 16(1)

Published: Jan. 5, 2022

Autologous bone grafts are commonly used as the gold standard to repair and regenerate diseased bones. However, they strongly associated with postoperative complications, especially at donor site, increased surgical costs. In an effort overcome these limitations, tissue engineering (TE) has been proposed alternative promote repair. The successful outcome of depends on microstructure composition materials scaffold. Decellularized matrix-based biomaterials have applied bioscaffolds in engineering. These play important role providing mechanical physical microenvironment needed by cells proliferate survive. extracellular matrix (dECM) can be a powder, hydrogel electrospun scaffolds. mimic native due their structure similar original tissue. aim this review is highlight decellularization techniques. Herein we discuss: (1) structure; (2) properties ideal scaffold; (3) potential decellularized bioscaffolds; (4) terminal sterilization bone; (5) cell removing confirmation tissues; (6) post procedures. Finally, improvement formation dECM immunogenicity aspect using presented, illustrate how novel dECM-based bioscaffold A comprehensive understanding may allow for better incorporation therapeutic approaches defects allowing regeneration.

Language: Английский

---

Multifunctional Hydroxyapatite Composites for Orthopedic Applications: A Review

ACS Biomaterials Science & Engineering, Journal Year: 2022, Volume and Issue: 8(8), P. 3162 - 3186

Published: July 15, 2022

Being a bioactive material, hydroxyapatite (HAp) is regarded as one of the most attractive ceramic biomaterials for bone and hard-tissue replacement regeneration. Despite its substantial biocompatibility, osteoconductivity, compositional similarity to that bone, employment HAp still limited in orthopedic applications due poor mechanical (low fracture toughness bending strength) antibacterial properties. These significant challenges lead notion developing novel HAp-based composites via different fabrication routes. HAp, when efficaciously combined with functionally graded materials agents, like Ag, ZnO, Co, etc., form render remarkable crack resistance toughening, well enhance bactericidal efficacy. The addition method, 3D printing, greatly influence porosity structure and, turn, control cell adhesion, thereby enabling biological fixation material. This article encompasses an elaborate discussion on multifunctional developed particular emphasis incorporation agents. printing scaffolds, vitro vivo studies conducted these, have all been included here. Furthermore, present review not only provides insights broad understanding by elucidating recent advancements toward 4D but also directs reader future research directions design application composite coatings scaffolds.

Language: Английский

---

Extracellular matrix dynamics: tracking in biological systems and their implications

Journal of Biological Engineering, Journal Year: 2022, Volume and Issue: 16(1)

Published: May 30, 2022

Abstract The extracellular matrix (ECM) constitutes the main acellular microenvironment of cells in almost all tissues and organs. ECM not only provides mechanical support, but also mediates numerous biochemical interactions to guide cell survival, proliferation, differentiation, migration. Thus, better understanding everchanging temporal spatial shifts composition structure – dynamics will provide fundamental insight regarding regulation tissue homeostasis how states transition from one another during diverse pathophysiological processes. This review outlines mechanisms mediating ECM-cell highlights changes modulate development disease progression, using lung as primary model organ. We then discuss existing methodologies for revealing compositional dynamics, with a particular focus on tracking newly synthesized proteins. Finally, we ramifications have engineering implement specific microenvironments into bioengineered tissues. Overall, this communicates current capabilities studying native delineates new research directions discovering implementing push frontier forward.

Language: Английский

---