3D extrusion bioprinting

Nature Reviews Methods Primers, Journal Year: 2021, Volume and Issue: 1(1)

Published: Nov. 11, 2021

Language: Английский

---

3D Bioprinting of Cell‐Laden Hydrogels for Improved Biological Functionality

Advanced Materials, Journal Year: 2021, Volume and Issue: 34(2)

Published: Oct. 20, 2021

Abstract The encapsulation of cells within gel‐phase materials to form bioinks offers distinct advantages for next‐generation 3D bioprinting. bioprinting has emerged as a promising tool patterning cells, but the technology remains limited in its ability produce biofunctional, tissue‐like constructs due dearth suitable bioinks. While early demonstrations commonly used viscous polymers optimized printability, these often lacked cell compatibility and biological functionality. In response, advanced that exist gel phase during entire printing process are being developed, since hydrogels uniquely positioned both protect extrusion provide signals embedded construct matures culture. Here, an overview design considerations is presented, with focus on their mechanical, biochemical, dynamic properties. Current challenges opportunities arise fact bioprinted active, living composed acellular cellular components also evaluated. Engineering consideration intrinsic component printed bioink will enable control over evolution after achieve greater biofunctionality.

Language: Английский

---

Hydrogels for Tissue Engineering: Addressing Key Design Needs Toward Clinical Translation

Frontiers in Bioengineering and Biotechnology, Journal Year: 2022, Volume and Issue: 10

Published: May 5, 2022

Graphical Abstract

Language: Английский

---

Advances in Gelatin Bioinks to Optimize Bioprinted Cell Functions

Advanced Healthcare Materials, Journal Year: 2023, Volume and Issue: 12(17)

Published: Feb. 19, 2023

Gelatin is a widely utilized bioprinting biomaterial due to its cell-adhesive and enzymatically cleavable properties, which improve cell adhesion growth. often covalently cross-linked stabilize bioprinted structures, yet the matrix unable recapitulate dynamic microenvironment of natural extracellular (ECM), thereby limiting functions cells. To some extent, double network bioink can provide more ECM-mimetic, niche for More recently, gelatin matrices are being designed using reversible cross-linking methods that emulate mechanical properties ECM. This review analyzes progress in developing formulations 3D culture, critically techniques, with focus on strategies optimize discusses new chemistries viscoelastic, stress-relaxing ECM, enable advanced functions, less explored engineering bioink. Finally, this work presents perspective areas future research argues next generation bioinks should be by considering cell-matrix interactions, constructs validated against currently established culture standards achieve improved therapeutic outcomes.

Language: Английский

---

Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion‐Volumetric Printing of Microgels

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(36)

Published: June 3, 2023

In living tissues, cells express their functions following complex signals from surrounding microenvironment. Capturing both hierarchical architectures at the micro- and macroscale, anisotropic cell patterning remains a major challenge in bioprinting, bottleneck toward creating physiologically-relevant models. Addressing this limitation, novel technique is introduced, termed Embedded Extrusion-Volumetric Printing (EmVP), converging extrusion-bioprinting layer-less, ultra-fast volumetric allowing spatially pattern multiple inks/cell types. Light-responsive microgels are developed for first time as bioresins (µResins) light-based providing microporous environment permissive homing self-organization. Tuning mechanical optical properties of gelatin-based microparticles enables use support bath suspended extrusion printing, which features containing high densities can be easily introduced. µResins sculpted within seconds with tomographic light projections into centimeter-scale, granular hydrogel-based, convoluted constructs. Interstitial microvoids enhanced differentiation stem/progenitor (vascular, mesenchymal, neural), otherwise not possible conventional bulk hydrogels. As proof-of-concept, EmVP applied to create synthetic biology-inspired intercellular communication models, where adipocyte regulated by optogenetic-engineered pancreatic cells. Overall, offers new avenues producing regenerative grafts biological functionality, developing engineered systems (metabolic) disease

Language: Английский

---

Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(52)

Published: April 23, 2023

Advances in bioprinting have enabled the fabrication of complex tissue constructs with high speed and resolution. However, there remains significant structural biological complexity within tissues that is unable to recapitulate. Bone, for example, has a hierarchical organization ranging from molecular whole organ level. Current techniques materials employed imposed limits on scale, speed, resolution can be achieved, rendering technique reproduce hierarchies cell-matrix interactions are observed bone. The shift toward biomimetic approaches bone engineering, where hydrogels provide biophysical biochemical cues encapsulated cells, promising approach enhancing function development vitro modeling. A major focus modeling creating dynamic microenvironmental niches support, stimulate, direct cellular processes formation remodeling. Hydrogels ideal imitating extracellular matrix since they engineered present various whilst allowing bioprinting. Here, recent advances 3D niche conducive engineering models reviewed.

Language: Английский

---

4D bioprinting of programmed dynamic tissues

Bioactive Materials, Journal Year: 2024, Volume and Issue: 37, P. 348 - 377

Published: April 23, 2024

Setting time as the fourth dimension, 4D printing allows us to construct dynamic structures that can change their shape, property, or functionality over under stimuli, leading a wave of innovations in various fields. Recently, smart biomaterials, biological components, and living cells into 3D constructs with effects has led an exciting field bioprinting. bioprinting gained increasing attention is being applied create programmed cell-laden such bone, cartilage, vasculature. This review presents overview on for engineering tissues organs, followed by discussion approaches, technologies, biomaterials design, bioink requirements, applications. While much progress been achieved, complex process facing challenges need be addressed transdisciplinary strategies unleash full potential this advanced biofabrication technology. Finally, we present future perspectives rapidly evolving bioprinting, view its potential, increasingly important roles development basic research, pharmaceutics, regenerative medicine.

Language: Английский

---

3D bioprinting advanced biomaterials for craniofacial and dental tissue engineering – A review

Materials & Design, Journal Year: 2024, Volume and Issue: 241, P. 112886 - 112886

Published: March 28, 2024

The rising incidence of defects in oral and maxillofacial tissues, linked to factors such as trauma, tumors, periodontal disease, aging, poses significant challenges. Current treatments, involving autografts, allografts, synthetic graft materials, face obstacles like secondary inflammation, inadequate biocompatibility. Tissue engineering, integrating cell biology material science since the 1990s, relies heavily on biomaterial scaffolds promote adhesion, proliferation, differentiation. Traditional scaffold fabrication, including 3D printing, methods lack precision, hindering effective tissue repair by controlling distribution extracellular matrix. Biomedical engineering advancements have introduced bioprinting an innovative solution, overcoming constraints conventional scaffolds. technology enables rapid precise reconstruction damaged tissues with loaded cells, mimicking vivo environments. This paper explores key technologies inkjet-based, extrusion-based, fused deposition modeling, laser-assisted, VAT photopolymerization, freeform reversible embedding suspended hydrogels, sacrificial template printing. selection materials suitable mechanical biological properties is crucial, considering distinct requirements each technique. review provides a comprehensive survey research progress printing applications craniofacial dental serving valuable reference for future medical research.

Language: Английский

---

Bioengineering methods for vascularizing organoids

Cell Reports Methods, Journal Year: 2024, Volume and Issue: 4(6), P. 100779 - 100779

Published: May 16, 2024

Organoids, self-organizing three-dimensional (3D) structures derived from stem cells, offer unique advantages for studying organ development, modeling diseases, and screening potential therapeutics. However, their translational ability to mimic complex in vivo functions are often hindered by the lack of an integrated vascular network. To address this critical limitation, bioengineering strategies rapidly advancing enable efficient vascularization organoids. These methods encompass co-culturing organoids with various cell types, lineage-specific organoids, co-differentiating cells into organ-specific lineages, using organoid-on-a-chip technology integrate perfusable vasculature within 3D bioprinting also create This review explores field organoid vascularization, examining biological principles that inform approaches. Additionally, envisions how converging disciplines biology, biomaterials, advanced fabrication technologies will propel creation increasingly sophisticated models, ultimately accelerating biomedical discoveries innovations.

Language: Английский

---

Biomaterials for extrusion-based bioprinting and biomedical applications

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: June 21, 2024

Amongst the range of bioprinting technologies currently available, by material extrusion is gaining increasing popularity due to accessibility, low cost, and absence energy sources, such as lasers, which may significantly damage cells. New applications extrusion-based are systematically emerging in biomedical field relation tissue organ fabrication. Extrusion-based presents a series specific challenges achievable resolutions, accuracy speed. Resolution particular paramount importance for realization microstructures (for example, vascularization) within tissues organs. Another major theme research cell survival functional preservation, extruded bioinks have cells subjected considerable shear stresses they travel through apparatus. Here, an overview main available printing related families materials (bioinks) provided. The achieving resolution whilst assuring viability function discussed application contexts

Language: Английский

---