Exposure to 6-PPD Quinone at Environmentally Relevant Concentrations Causes Abnormal Locomotion Behaviors and Neurodegeneration in Caenorhabditis elegans

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(12), P. 4940 - 4950

Published: March 13, 2023

6-PPD quinone (6-PPDQ) can be transformed from through ozonation. Nevertheless, the potential neurotoxicity of 6-PPDQ after long-term exposure and underlying mechanism are largely unclear. In Caenorhabditis elegans, we here observed that 0.1–10 μg/L caused several forms abnormal locomotion behaviors. Meanwhile, neurodegeneration D-type motor neurons was in 10 6-PPDQ-exposed nematodes. The associated with activation Ca2+ channel DEG-3-mediated signaling cascade. this cascade, expressions deg-3, unc-68, itr-1, crt-1, clp-1, tra-3 were increased by 6-PPDQ. Moreover, among genes encoding neuronal signals required for control stress response, jnk-1 dbl-1 decreased 6-PPDQ, daf-7 glb-10 RNAi jnk-1, dbl-1, daf-7, resulted susceptibility to toxicity decreasing locomotory ability inducing neurodegeneration, suggesting JNK-1, DBL-1, DAF-7, GLB-10 also induction neurotoxicity. Molecular docking analysis further demonstrated binding DEG-3, GLB-10. Together, our data suggested risk at environmentally relevant concentrations causing organisms.

Language: Английский

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Benzimidazole Fungicide Carbendazim Induces Gut Inflammation through the TLR5/NF-κB Pathway in Grass Carp

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Fungicides have been increasingly used across various sectors, including agriculture and textiles. The biocidal properties of fungicides may negatively impact the stability intestinal microbiota, thereby posing a threat to health. In this study, we investigated mechanisms damage functional abnormalities in grass carp following 42-day exposure widely fungicide carbendazim at environmentally relevant concentrations (0.2 20 μg/L). Histopathological observations, mRNA protein expression analyses, biochemical analysis, quantification short-chain fatty acids (SCFAs), cytokines, lipopolysaccharide (LPS), 16S ribosomal ribonucleic acid (rRNA), as well internal transcribed spacer (ITS) sequencing, were performed. At concentrations, strongly induced inflammation, leading increased transcriptional translational levels genes involved toll-like receptor five (TLR5)/nuclear factor kappa B (NF-κB) pathway, TLR5, NF-κB, interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNFα). Additionally, damaged barriers reduced tight junction proteins (e.g., occludin zonula occludens-1/2), goblet cells, immunoglobulin M levels, while also disrupting gut microbiome, metabolic disorders, particularly decreases SCFAs increases LPS. Treatment with TLR5 antagonist TH1020 mitigated inflammation caused by carbendazim, subsequently improving mechanical barrier function. Overall, our findings provide new insights into toxicological underlying carp, indicating that poses significant nontarget organisms. Given its widespread detection environment, these results underscore substantial ecological risks health fish living carbendazim-contaminated water bodies.

Language: Английский

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A review of the toxic effects of ammonia on invertebrates in aquatic environments

Environmental Pollution, Journal Year: 2023, Volume and Issue: 336, P. 122374 - 122374

Published: Aug. 25, 2023

Language: Английский

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Bisphenol A Analogues Induce Neuroendocrine Disruption via Gut–Brain Regulation in Zebrafish

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(2), P. 1022 - 1035

Published: Jan. 2, 2024

There is epidemiological evidence in humans that exposure to endocrine-disrupting chemicals such as bisphenol A (BPA) tied abnormal neuroendocrine function with both behavioral and intestinal symptoms. However, the underlying mechanism of this effect, particularly role gut–brain regulation, poorly understood. We exposed zebrafish embryos a concentration series (including environmentally relevant levels) BPA its analogues. The analogue G (BPG) yielded strongest impact on larvae inhibited largest number neurotransmitters, an effective 0.5 μg/L, followed by AF (BPAF) BPA. In neurod1:EGFP transgenic zebrafish, BPG BPAF distribution enteroendocrine cells (EECs), which associated decreased neurotransmitters level activity. Immune staining ace-α-tubulin suggested vagal neural development at 50 500 μg/L. Single-cell RNA-Seq demonstrated disrupted system inducing inflammatory responses epithelial via TNFα-trypsin-EEC signaling. activated apoptosis developmental pathways neurons, consistent immunofluorescence imaging studies. These findings show affect through axis but different mechanisms, revealing new insights into modes bisphenol-mediated disruption.

Language: Английский

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An azole fungicide climbazole damages the gut-brain axis in the grass carp

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 465, P. 133463 - 133463

Published: Jan. 10, 2024

Language: Английский

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Flunitrazepam and its metabolites induced brain toxicity: Insights from molecular dynamics simulation and transcriptomic analysis

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 465, P. 133113 - 133113

Published: Nov. 29, 2023

Language: Английский

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Maternal or Paternal Antibiotics? Intergenerational Transmission and Reproductive Toxicity in Zebrafish

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 58(2), P. 1287 - 1298

Published: Dec. 19, 2023

Despite the known direct toxicity of various antibiotics to aquatic organisms, potential chronic impact through intergenerational transmission on reproduction remains elusive. Here, we exposed zebrafish a mixture 15 commonly consumed at environmentally relevant concentrations (1 and 100 μg L–1) with cross-mating design. A high accumulation was detected in ovary (up 904.58 ng g–1) testis 1704.49 F0 fish. The from generation subsequent (F1 offspring) confirmed rate (ki) ranging 0.11 2.32. maternal transfer significantly higher, relative paternal transfer, due greater role ovarian enrichment oviposition compared enrichment. There were similar impairments reproductive developmental indexes F1 eggs found following both female male parental exposure. Almost all eliminated F2 comparison eggs. However, there still toxic responses observed fish, suggesting that antibiotic concentration levels not only criterion for evaluating effects each generation. These findings unveil mechanism fish models underscore their lasting environments.

Language: Английский

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Inflammation and cardiometabolic diseases induced by persistent organic pollutants and nutritional interventions: Effects of multi-organ interactions

Environmental Pollution, Journal Year: 2023, Volume and Issue: 339, P. 122756 - 122756

Published: Oct. 14, 2023

Language: Английский

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Gut microbiota dysbiosis triggered by salinity stress enhances systemic inflammation in spotted scat (Scatophagus argus)

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110353 - 110353

Published: April 1, 2025

Language: Английский

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Mechanisms of neurocentral-eyestalk-intestinal immunotoxicity in whiteleg shrimp Litopenaeus vannamei under ammonia nitrogen exposure

Environmental Pollution, Journal Year: 2024, Volume and Issue: 349, P. 123956 - 123956

Published: April 14, 2024

Language: Английский

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