Wastewater-based estimation of temporal variation in shedding amount of influenza A virus and clinically identified cases using the PRESENS model DOI Creative Commons
Hiroki Ando, Michio Murakami, Masaaki Kitajima

et al.

Environment International, Journal Year: 2024, Volume and Issue: 195, P. 109218 - 109218

Published: Dec. 19, 2024

Language: Английский

Detection of Hemagglutinin H5 Influenza A Virus Sequence in Municipal Wastewater Solids at Wastewater Treatment Plants with Increases in Influenza A in Spring, 2024 DOI Creative Commons
Marlene K. Wolfe,

Dorothea Duong,

Bridgette Shelden

et al.

Environmental Science & Technology Letters, Journal Year: 2024, Volume and Issue: 11(6), P. 526 - 532

Published: May 20, 2024

Prospective influenza A (IAV) RNA monitoring at 190 wastewater treatment plants (WWTPs) across the US identified increases in IAV concentrations 59 spring 2024, after typical seasonal period, coincident with identification of highly pathogenic avian (subtype H5N1) circulating dairy cattle US. We developed and validated a hydrolysis-probe RT-PCR assay for quantification H5 hemagglutinin gene. applied it retrospectively to samples from four WWTPs where springtime were one WWTP they not. The marker was detected all coinciding not without an increase. Positive are located states confirmed outbreaks influenza, H5N1 clade 2.3.4.4b, cattle. Concentrations gene approached overall virus concentrations, suggesting large fraction inputs subtypes. At positive WWTPs, industrial discharges containing animal waste, including milk byproducts, permitted discharge into sewers. Our findings demonstrate that can detect animal-associated contributions highlight need consider agricultural wastewater. This work illustrates monitoring's value comprehensive surveillance, influenzas currently thought be primarily found animals important implications human health.

Language: Английский

Citations

26
Observations of Respiratory Syncytial Virus (RSV) Nucleic Acids in Wastewater Solids Across the United States in the 2022–2023 Season: Relationships with RSV Infection Positivity and Hospitalization Rates DOI Creative Commons
Alessandro Zulli, Meri R.J. Varkila, Julie Parsonnet

et al.

ACS ES&T Water, Journal Year: 2024, Volume and Issue: 4(4), P. 1657 - 1667

Published: Feb. 29, 2024

Respiratory syncytial virus (RSV) is a leading cause of respiratory illness and hospitalization, but clinical surveillance detects only minority cases. Wastewater could determine the onset extent RSV circulation in absence sensitive case detection, to date, studies wastewater are few. We measured RNA concentrations solids from 176 sites during 2022–2023 season compared those publicly available infection positivity hospitalization rates. Concentrations ranged undetectable 107 copies per gram. concentration aggregated at state national levels correlated with was determined using both rates independent algorithms for 14 states where data were start season. In 4 states, identified same week; 3 preceded onset, 7 occurred after onset. generally peaked week as peaked. Differences peaks versus may reflect inherent differences approaches.

Language: Английский

Citations

13
Wastewater Surveillance for Influenza A Virus and H5 Subtype Concurrent with the Highly Pathogenic Avian Influenza A(H5N1) Virus Outbreak in Cattle and Poultry and Associated Human Cases — United States, May 12–July 13, 2024 DOI Open Access

Souci Louis,

Miguella Mark‐Carew, Matthew Biggerstaff

et al.

MMWR Morbidity and Mortality Weekly Report, Journal Year: 2024, Volume and Issue: 73(37), P. 804 - 809

Published: Sept. 19, 2024

As part of the response to highly pathogenic avian influenza A(H5N1) virus outbreak in U.S. cattle and poultry associated human cases, CDC partners are monitoring A levels detection H5 subtype wastewater. Among 48 states District Columbia that performed testing wastewater during May 12-July 13, 2024, a weekly average 309 sites 38 had sufficient data for analysis, 11 four reported high virus. was conducted at 203 41 states, with detections 24 nine states. For each or level, state local health departments evaluated from other surveillance systems partnered utilities agriculture investigate potential sources. detected wastewater, three corresponding evidence activity systems. detections, 15 identified animal sources within sewershed adjacent county, including eight milk-processing inputs. Data these early investigations can help officials optimize use upcoming respiratory illness season.

Language: Английский

Citations

12
Early Detection of the Emerging SARS-CoV-2 BA.2.86 Lineage Through Wastewater Surveillance Using a Mediator Probe PCR Assay — Shenzhen City, Guangdong Province, China, 2023 DOI Creative Commons
Du Chen,

Yuejing Peng,

Ziquan Lyu

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 6(15), P. 332 - 338

Published: Jan. 1, 2024

Introduction The emergence of the new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron sublineage, BA.2.86, has sparked global public health concerns for its potential heightened transmissibility and immune evasion. Utilizing data from Shenzhen's city-wide wastewater surveillance system, we highlight presence BA.2.86 lineage in Shenzhen. Methods A mediator probe polymerase chain reaction (PCR) assay was developed to detect by targeting a specific mutation (Spike: A264D). Between September 19 December 10, 2023, 781 samples 38 treatment plants (WWTPs) 9 pump stations ten districts Shenzhen were examined. Through multiple short-amplicon sequencing, three positive identified. Results identified Futian Nanshan on 2, 2023. From total 21 BA.2.86-positive found across 6 (Futian, Nanshan, Longhua, Baoan, Longgang, Luohu) weighted average viral load 43.5 copies/L increased 219.8 4, then decreased approximately 100 6, 8, 10. Conclusions PCR assay, designed swift detection low concentrations samples, shows promise detecting different SARS-CoV-2 variants. Wastewater could serve as an early system promptly identifying variants they emerge.

Language: Английский

Citations

7
Human pathogen nucleic acids in wastewater solids from 191 wastewater treatment plants in the United States DOI Creative Commons
Alexandria B. Boehm, Marlene K. Wolfe, Amanda L. Bidwell

et al.

Scientific Data, Journal Year: 2024, Volume and Issue: 11(1)

Published: Oct. 17, 2024

We measured concentrations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its variants, influenza A B viruses, syncytial virus, human metapneumovirus, enterovirus D68, parainfluenza types 1, 2, 3, 4a, 4b in aggregate, norovirus genotype II, rotavirus, Candida auris, hepatitis adenovirus, mpox H5 pepper mild mottle virus nucleic acids wastewater solids prospectively at 191 treatment plants 40 states across the United States plus Washington DC. Measurements were made two to seven times per week from 1 January 2022 30 June 2024, depending on plant staff availability. using droplet digital (reverse-transcription-) polymerase chain reaction (ddRT-PCR) following best practices for making environmental molecular biology measurements. These data can be used better understand disease occurrence communities contributing wastewater.

Language: Английский

Citations

6
Detection of hemagglutinin H5 influenza A virus sequence in municipal wastewater solids at wastewater treatment plants with increases in influenza A in spring, 2024 DOI
Marlene K. Wolfe,

Dorothea Duong,

Bridgette Shelden

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 29, 2024

Abstract Prospective influenza A (IAV) RNA monitoring at 190 wastewater treatment plants across the US identified increases in IAV concentrations 59 spring 2024, after typical seasonal period, coincident with identification of highly pathogenic avian (subtype H5N1) circulating dairy cattle US. We developed and validated a hydrolysis-probe RT-PCR assay for quantification H5 hemagglutinin gene. applied it retrospectively to samples from three where springtime were identified. The marker was detected all coinciding increases. Plants located state confirmed outbreaks influenza, H5N1 clade 2.3.4.4b, cattle. Concentrations gene approached overall virus concentrations, suggesting large fraction inputs subtypes. At two plants, industrial discharges containing animal waste, including milk byproducts, permitted discharge into sewers. Our findings demonstrate can detect animal-associated contributions, highlight need consider agricultural wastewater. This work illustrates monitoring’s value comprehensive surveillance diseases zoonotic potential human populations.

Language: Английский

Citations

4
Challenges and Opportunities for Wastewater Monitoring of Influenza Viruses During the Multistate Outbreak of Highly Pathogenic Avian Influenza A(H5N1) Virus in Dairy Cattle and Poultry DOI
Margaret A. Honein, Sonja J. Olsen, Daniel B. Jernigan

et al.

American Journal of Public Health, Journal Year: 2024, Volume and Issue: unknown, P. e1 - e4

Published: Sept. 19, 2024

Language: Английский

Citations

4
Estimating epidemic trajectories of SARS-CoV-2 and influenza A virus based on wastewater monitoring and a novel machine learning algorithm DOI
Songzhe Fu, Yixiang Zhang,

Yinghui Li

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175830 - 175830

Published: Aug. 27, 2024

Language: Английский

Citations

3
Respiratory Virus Season Surveillance in the United States Using Wastewater Metrics, 2023–2024 DOI Creative Commons
Elana M. G. Chan, Alexandria B. Boehm

ACS ES&T Water, Journal Year: 2025, Volume and Issue: 5(2), P. 985 - 992

Published: Jan. 22, 2025

Wastewater measurements represent an entire contributing population and can be available within 24 h. Enhanced information about disease occurrence improve health through better timing of policies interventions. We aimed to infer seasonal patterns for common respiratory viruses alongside transmission dynamics SARS-CoV-2 across the USA using wastewater samples. used RNA concentrations influenza A B (IAV/IBV), syncytial virus (RSV), human metapneumovirus (HMPV), from 175 treatment plants (July 2023–June 2024). For IAV, IBV, RSV, HMPV, we determined epidemic onset, offset, peak, duration at national subnational scales. SARS-CoV-2, categorized based on recent levels trends. Epidemic onset occurred in November IAV RSV which aligned with prepandemic norms. Onset January IBV April HMPV were later than expected according historical data. Duration was longer shorter peak dates consistent norms all viruses. Peak coincided high, upward trending concentrations, suggesting potential co-occurrence these

Language: Английский

Citations

0
Influenza RNA fluxes monitoring in wastewater as a complementary epidemiological surveillance indicator: A four-year nationwide study in Luxembourg DOI Creative Commons
Manon Chassaing, Cécile Walczak, Aurélie Sausy

et al.

The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 982, P. 179621 - 179621

Published: May 13, 2025

Wastewater surveillance has demonstrated success in monitoring SARS-CoV-2 communities, indicating potential for extension to other respiratory viruses. This study investigates influenza A and B viruses (IAV; IBV) raw urban wastewater over a 4-year period, introducing two key concepts: the use of viral RNA fluxes instead concentration measurements determination epidemiological parameters directly from data. The estimation daily fluxes, representing number genome copies per day 100,000 inhabitants, offers an integrative approach that combines microbiological hydrological better assess particle dynamics water system. total 1013 samples collected between March 2020 2024 Luxembourg's four largest treatment plants (covering about 52 % population) were analysed using RT-qPCR RT-droplet digital PCR (RT-ddPCR), following particles by ultrafiltration. Data on presence IAV IBV expressed as either detection rates or fluxes. Significant correlations observed laboratory-confirmed cases both (RT-qPCR: Spearman ρ = 0.52; RT-ddPCR: 0.61, p-value <10-13) (RT-ddPCR: 0.64, <10-15). More importantly, our results critical seasonality (start, peak end weeks epidemic) can be effectively determined These findings establish cost-effective, non-invasive support complement existing programs, with applications pathogens.

Language: Английский

Citations

0