Effects of DNA Damage and Oxidative Stress in Human Bronchial Epithelial Cells Exposed to PM2.5 from Beijing, China, in Winter DOI Open Access

Bingyu Niu,

Wenke Li, Jiangshuai Li

et al.

International Journal of Environmental Research and Public Health, Journal Year: 2020, Volume and Issue: 17(13), P. 4874 - 4874

Published: July 6, 2020

Epidemiological studies have corroborated that respiratory diseases, including lung cancer, are related to fine particulate matter (<2.5 μm) (PM2.5) exposure. The toxic responses of PM2.5 greatly influenced by the source PM2.5. However, effects from Beijing on bronchial genotoxicity scarce. In present study, was sampled and applied in vitro investigate its mechanisms behind it. Human epithelial cells 16HBE were used as a model for Low (67.5 μg/mL), medium (116.9 high (202.5 μg/mL) doses cell After exposure, viability, oxidative stress markers, DNA (deoxyribonucleic acid) strand breaks, 8-OH-dG levels, micronuclei formation, repair gene expression measured. results showed significantly induced cytotoxicity 16HBE. Moreover, levels reactive oxygen species (ROS), malondialdehyde (MDA), cellular heme oxygenase (HO-1) increased, level glutathione (GSH) decreased, which represented occurrence severe micronucleus rate elevated, damage occurred indicators comet assay, γ-H2AX 8-OH-dG, markedly enhanced PM2.5, accompanied influence 8-oxoguanine glycosylase (OGG1), X-ray cross-complementing 1 (XRCC1), poly (ADP-ribose) polymerase-1 (PARP1) expression. These support significant role cells, may occur through combined effect genes.

Language: Английский

Study on the oxidation potential of the water-soluble components of ambient PM2.5 over Xi’an, China: Pollution levels, source apportionment and transport pathways DOI Creative Commons
Yuqin Wang,

Mamin Wang,

Shengping Li

et al.

Environment International, Journal Year: 2020, Volume and Issue: 136, P. 105515 - 105515

Published: Jan. 29, 2020

Reactive oxygen species (ROS) are a class of substances that general concern in terms human health and used to represent the oxidation potential (OP) atmosphere. In this study, ROS levels 116 daily fine particulate matter (PM2.5) samples taken over Xi'an 2017 were measured with dithiothreitol (DTT) method. The sources DTTv (volume-based DTT consumption) PM2.5 as well their contributions identified by both positive matrix factorization (PMF) multiple linear regression (MLR) based on chemical (PM). results showed yearly average was 0.53 nmol/min/m3 (0.19-1.10 nmol/min/m3). highest level occurred winter, followed spring, summer autumn. most strongly correlated water-soluble organic carbon (WSOC; r = 0.85), but effects WSOC very limited. SO2, NO2, CO, elemental (EC) K+ (r > 0.64) had moderate correlations moderately related environmentally persistent free radicals (EPFRs) 0.56). mixed-effects model pollutants originating from incomplete combustion greater than those complete combustion. Source apportionment PMF motor vehicle emissions (27.4%), secondary sulfates (21.6%) coal (18.8%) more important contributors dust (8.4%), metal processing (4.9%), industrial (11.3%) nitrates (7.5%). for consistent MLR results, which verified feasible methods source specific such EPFRs. Backward trajectory clusters dominant cluster groups local regional transport, while OP affected long-range transport transport. As stated above, improvement atmospheric require not only efforts also large-scale joint cooperation. Furthermore, study PM information provides guidance effect research.

Language: Английский

Citations

112
Fine particular matter and its constituents in air pollution and gestational diabetes mellitus DOI Creative Commons

Guoqi Yu,

Junjie Ao, Jing Cai

et al.

Environment International, Journal Year: 2020, Volume and Issue: 142, P. 105880 - 105880

Published: June 25, 2020

Ambient air pollution has been linked to the development of gestational diabetes mellitus (GDM). However, previous studies provided inconsistent findings and no study examined effects complex chemical constituents particular matter on GDM, especially in developing countries. Therefore, we aim investigate associations exposure PM2.5 (particular ≤ 2.5 μm) its with identify susceptible window a large survey China.The China Labor Delivery Survey was cross-sectional investigation conducted 24 provinces between 2015 2016. A random sample all deliveries each participating hospital selected detailed obstetric newborn information extracted from medical records. Average concentrations six (organic matter, black carbon, sulfate, nitrate, ammonium soil dust) were estimated (1 km × 1 km) using combined geoscience-statistical model. GDM diagnosed based an oral glucose tolerance test (OGTT) 28 weeks gestation according IADPSG criteria. Generalized linear mixed models used adjust for potential confounders.A total 54,517 subjects 55 hospitals included. The incidence 10.8%. An interquartile range (IQR) increase 2nd trimester pregnancy associated increased risk single pollutant model, [adjusted odds ratio (aOR) = 1.11 95% confidence interval (CI): 1.01-1.22]. Exposure organic (aOR 1.14; 95%CI: 1.05-1.23), carbon 1.15; 1.07-1.25) nitrate 1.13; 1.02-1.24) during risks GDM. Associations robust after controlling mass accounting multi-collinearity.Exposure Organic may be main culprits association.

Language: Английский

Citations

82
Radical Formation by Fine Particulate Matter Associated with Highly Oxygenated Molecules DOI
Haijie Tong, Yun Zhang, Alexander Filippi

et al.

Environmental Science & Technology, Journal Year: 2019, Volume and Issue: 53(21), P. 12506 - 12518

Published: Sept. 19, 2019

Highly oxygenated molecules (HOMs) play an important role in the formation and evolution of secondary organic aerosols (SOA). However, abundance HOMs different environments their relation to oxidative potential fine particulate matter (PM) are largely unknown. Here, we investigated relative HOM radical yield laboratory-generated SOA PM ambient air ranging from remote forest areas highly polluted megacities. By electron paramagnetic resonance mass spectrometric investigations, found that HOMs, especially dimeric low-volatility types, was positively correlated with radicals aqueous extracts. photooxidation isoprene, ozonolysis α- β-pinene, tropical (central Amazon) boreal (Hyytiälä, Finland) forests exhibited a higher than naphthalene urban sites (Beijing, Guangzhou, Mainz, Shanghai, Xi'an), confirming constituents biogenic generate radicals. Our study provides new insights into chemical relationship abundance, composition, sources by laboratory aerosols, enabling better quantification component-specific contribution source- or site-specific its climate health effects.

Language: Английский

Citations

76
The Lung Microbiota Affects Pulmonary Inflammation and Oxidative Stress Induced by PM2.5 Exposure DOI
Simin Wang, Qixing Zhou, Yingze Tian

et al.

Environmental Science & Technology, Journal Year: 2022, Volume and Issue: 56(17), P. 12368 - 12379

Published: Aug. 19, 2022

Fine particulate matter (PM2.5) exposure causes respiratory diseases by inducing inflammation and oxidative stress. However, the correlation between pulmonary microbiota progression of stress caused PM2.5 is poorly understood. This study tested hypothesis that lung affects induced exposure. Mice were exposed to intranasally for 12 days. Then, transfer antibiotic intervention performed. Histological examinations, biomarker index detection, transcriptome analyses conducted. Characterization using 16S rRNA gene sequencing showed its diversity decreased 75.2% in PM2.5-exposed mice, with increased abundance Proteobacteria Bacteroidota. The altered composition was significantly correlated stress-related indicators. Intranasal from mice affected PM2.5, as shown proinflammatory cytokine levels dysregulated damage-related biomarkers. Antibiotic during alleviated damage mice. also substantial changes after treatment, reflected diversity, These results suggest microbial dysbiosis can promote affect

Language: Английский

Citations

53
PM2.5 air pollution prediction through deep learning using meteorological, vehicular, and emission data: A case study of New Delhi, India DOI
Deepti Shakya, Vishal Deshpande, Manish Kumar Goyal

et al.

Journal of Cleaner Production, Journal Year: 2023, Volume and Issue: 427, P. 139278 - 139278

Published: Oct. 13, 2023

Language: Английский

Citations

26
Urban airborne PM2.5 induces pulmonary fibrosis through triggering glycolysis and subsequent modification of histone lactylation in macrophages DOI Creative Commons
Jingyi Li, Guodong Zeng, Zezhong Zhang

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 273, P. 116162 - 116162

Published: March 1, 2024

Airborne fine particulate matter (PM2.5) can cause pulmonary inflammation and even fibrosis, however, the underlying molecular mechanisms of pathogenesis PM2.5 exposure have not been fully appreciated. In present study, we explored dynamics glycolysis modification histone lactylation in macrophages induced by PM2.5-exposure both vivo vitro models. Male C57BL/6 J mice were anesthetized administrated with intratracheal instillation once every other day for 4 weeks. Mouse RAW264.7 alveolar epithelial MLE-12 cells treated 24 h. We found that significantly increased lactate dehydrogenase (LDH) activities contents, up-regulated mRNA expression key glycolytic enzymes lungs bronchoalveolar lavage fluids mice. Moreover, levels PM2.5-exposed cells. The pro-fibrotic cytokines secreted from PM2.5-treated triggered epithelial-mesenchymal transition (EMT) through activating transforming growth factor-β (TGF-β)/Smad2/3 VEGFA/ERK pathways. contrast, LDHA inhibitor (GNE-140) pretreatment effectively alleviated PM2.5-induced fibrosis via inhibiting subsequent Thus, our findings suggest play critical role PM2.5-associated fibrosis.

Language: Английский

Citations

13
Toxic Potencies of Particulate Matter from Typical Industrial Plants Mediated with Acidity via Metal Dissolution DOI

Xiwen Song,

Di Wu, Chen Xiu

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(15), P. 6736 - 6743

Published: April 2, 2024

Acidity is an important property of particulate matter (PM) in the atmosphere, but its association with PM toxicity remains unclear. Here, this study quantitively reports effect acidity level on via pH-control experiments and cellular analysis. Oxidative stress cytotoxicity potencies acidified samples at pH 1–2 were up to 2.8–5.2 2.1–13.2 times higher than those 8–11, respectively. The toxic from real-world smoke plumes 2.3 9.1–18.2 greater 5.6, demonstrating a trend similar that samples. Furthermore, impact was manifested by promoting metal dissolution. dramatic increase 2–3 orders magnitude water-soluble content dominated variation toxicity. significant correlation between sulfate, value, Fe, IC20, EC1.5 (p < 0.05) suggested acidic sulfate could enhance dissolving insoluble metals. findings uncover superficial adverse health outcomes epidemiological research highlight control wet plume emissions mitigate effects acidity.

Language: Английский

Citations

13
Variable Valence State of Trace Elements Regulating Toxic Potencies of Inorganic Particulate Matter DOI
Chen Xiu, Di Wu,

Lixin Zheng

et al.

Environmental Science & Technology Letters, Journal Year: 2024, Volume and Issue: 11(3), P. 223 - 229

Published: Feb. 9, 2024

Trace element is known to be one major component in determining particulate matter (PM) toxicities. However, there still no accurate assessment of the toxic potency mixed valences. Here, we reported oxidative stress and cytotoxicity potencies 14 trace elements their various valence states estimated gaps inorganic PM resulting from variations Substantial discrepancies up 3 orders magnitude were observed among different When considering abundance PM, toxicity are range 5 6 times between greatest weakest emitted industrial sources, with iron contributing 65.5%–91.0% overall gaps. Furthermore, relative variation shows a significant correlation additive toxicities Fe(II) Fe(III) ions during aging process. The finding highlights that multiple coexisting need taken into account when estimating potencies.

Language: Английский

Citations

9
Hazardous trace elements in thoracic fraction of airborne particulate matter: Assessment of temporal variations, sources, and health risks in a megacity DOI
Omar Ramírez, Ana M. Sánchez de la Campa, Daniel Sánchez-Rodas

et al.

The Science of The Total Environment, Journal Year: 2019, Volume and Issue: 710, P. 136344 - 136344

Published: Dec. 28, 2019

Language: Английский

Citations

72
Health risk-oriented source apportionment of PM2.5-associated trace metals DOI
Jiawen Xie, Ling Jin, Jinli Cui

et al.

Environmental Pollution, Journal Year: 2020, Volume and Issue: 262, P. 114655 - 114655

Published: April 23, 2020

Language: Английский

Citations

70