Wastewater concentrations of human influenza, metapneumovirus, parainfluenza, respiratory syncytial virus, rhinovirus, and seasonal coronavirus nucleic-acids during the COVID-19 pandemic: a surveillance study

The Lancet Microbe, Journal Year: 2023, Volume and Issue: 4(5), P. e340 - e348

Published: March 22, 2023

Respiratory disease is a major cause of morbidity and mortality; however, surveillance for circulating respiratory viruses passive biased. Wastewater-based epidemiology has been used to understand SARS-CoV-2, influenza A, syncytial virus (RSV) infection rates at community level but not investigate other viruses. We aimed use wastewater-based viral occurrence.A retrospective study was carried out large wastewater treatment plant located in California, USA. Using droplet digital RT-PCR, we measured RNA concentrations A B viruses, RSV B, parainfluenza (1-4) rhinovirus, seasonal coronaviruses, metapneumovirus solids three times per week 17 months (216 samples) between Feb 1, 2021, June 21, 2022. Novel probe-based RT-PCR assays non-influenza targets were developed validated. compared positivity infections from clinical specimens submitted California Sentinel Clinical Laboratories (sentinel laboratories) assess concordance the two datasets.We detected all tested solids. Human rhinovirus (median concentration 4300 [0-9500] copies gram dry weight) human coronaviruses (35 000 [17 000-56 000]) found highest concentrations. Concentrations correlated significantly positively with associated diseases sentinel laboratories (tau 0·32-0·57, p<0·0009); only exceptions which rarely Measurements indicated coronavirus OC43 dominated whereas 3 among during period. decreased noticeably after omicron BA.1 surge suggesting connection changes behaviour transmission viruses.Wastewater-based can be obtain information on circulation localised, without need test many individuals because single sample represents entire contributing community. Results available within 24 h collection, generating real time inform public health responses, decision making, individual modifications.CDC Foundation.

Language: Английский

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Wastewater-Based Detection of Two Influenza Outbreaks

Environmental Science & Technology Letters, Journal Year: 2022, Volume and Issue: 9(8), P. 687 - 692

Published: July 5, 2022

Traditional influenza surveillance informs control strategies but can lag behind outbreak onset and undercount cases. Wastewater is effective for monitoring near real-time dynamics of outbreaks has not been attempted influenza. We quantified A virus (IAV) RNA in wastewater during two active on university campuses different parts the United States times year using case data from an investigation high-quality student athletes. In both cases, IAV concentrations were strongly associated with reported incidence rates (Kendall's τ values 0.58 0.67 University Michigan Stanford University, respectively). Furthermore, reflected patterns magnitudes. For outbreak, evidence sequencing indicated same circulating strain identified cases outbreak. The results demonstrate that effectively detect will therefore be a valuable supplement to traditional forms surveillance.

Language: Английский

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Wastewater Surveillance for Infectious Disease: A Systematic Review

American Journal of Epidemiology, Journal Year: 2022, Volume and Issue: 192(2), P. 305 - 322

Published: Oct. 13, 2022

Abstract Wastewater surveillance for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been shown to be a valuable source of information regarding SARS-CoV-2 transmission and disease 2019 (COVID-19) cases. Although the method used several decades track other infectious diseases, there not comprehensive review outlining all pathogens that have surveilled through wastewater. Herein we identify diseases previously studied via wastewater prior COVID-19 pandemic. Infectious were identified in 100 studies across 38 countries, as themes how measures linked. Twenty-five separate pathogen families included studies, with majority examining from family Picornaviridae, including polio nonpolio enteroviruses. Most did link what was found transmission. Among those did, value reported varied by study. should considered potential public health tool many diseases. can improved incorporating at population-level incidence hospitalizations.

Language: Английский

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Use of Wastewater for Mpox Outbreak Surveillance in California

New England Journal of Medicine, Journal Year: 2023, Volume and Issue: 388(6), P. 570 - 572

Published: Jan. 18, 2023

Language: Английский

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Occurrence of multiple respiratory viruses in wastewater in Queensland, Australia: Potential for community disease surveillance

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 864, P. 161023 - 161023

Published: Dec. 18, 2022

The early warning and tracking of COVID-19 prevalence in the community provided by wastewater surveillance has highlighted its potential for much broader viral disease surveillance. In this proof-of-concept study, 46 samples from four treatment plants (WWTPs) Queensland, Australia, were analyzed presence abundance 13 respiratory viruses, results compared with reported clinical cases. viruses concentrated using adsorption-extraction (AE) method, extracted nucleic acids qPCR RT-qPCR. Among tested, bocavirus (BoV), parechovirus (PeV), rhinovirus A (RhV A) B B) detected all samples. All tested except influenza virus (IBV) sample at least one WWTP. BoV was greatest concentration (4.96-7.22 log

Language: Английский

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Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond

Clinical Microbiology Reviews, Journal Year: 2023, Volume and Issue: 37(1)

Published: Dec. 14, 2023

SUMMARY Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context of coronavirus disease 2019 (COVID-19) pandemic. The power and potential this platform technology were rapidly realized when it became evident that not only did WBS-measured SARS-CoV-2 RNA correlate strongly with COVID-19 clinical within monitored populations but also, fact, functioned as a leading indicator. Teams from across globe innovated novel approaches by which wastewater could be collected diverse sewersheds ranging treatment plants (enabling community-level surveillance) to more granular locations including individual neighborhoods high-risk buildings such long-term care facilities (LTCF). Efficient processes enabled extraction concentration highly dilute matrix. Molecular genomic tools identify, quantify, characterize its various variants adapted programs applied these mixed environmental systems. Novel data-sharing allowed information mobilized made immediately available public health government decision-makers even public, enabling evidence-informed decision-making based on local dynamics. WBS since been recognized tool transformative potential, providing near-real-time cost-effective, objective, comprehensive, inclusive data changing prevalence measured analytes space time populations. However, consequence rapid innovation hundreds teams simultaneously, tremendous heterogeneity currently exists literature. This manuscript provides state-of-the-art review established details current work underway expanding scope other infectious targets.

Language: Английский

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Monitoring influenza and respiratory syncytial virus in wastewater. Beyond COVID-19

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 892, P. 164495 - 164495

Published: May 26, 2023

Wastewater-based surveillance can be a valuable tool to monitor viral circulation and serve as an early warning system. For respiratory viruses that share similar clinical symptoms, namely SARS-CoV-2, influenza, syncytial virus (RSV), identification in wastewater may allow differentiation between seasonal outbreaks COVID-19 peaks. In this study, these well standard indicators of fecal contamination, weekly sampling campaign was carried out for 15 months (from September 2021 November 2022) two treatment plants the entire population Barcelona (Spain). Samples were concentrated by aluminum hydroxide adsorption-precipitation method then analyzed RNA extraction RT-qPCR. All samples positive while positivity rates influenza RSV significantly lower (10.65 % A (IAV), 0.82 B (IBV), 37.70 RSV-A 34.43 RSV-B). Gene copy concentrations SARS-CoV-2 often approximately 1 2 logarithmic units higher compared other viruses. Clear peaks IAV H3:N2 February March 2022 winter observed, which matched chronological incidence infections recorded Catalan Government database. conclusion, data obtained from provided new information on abundance area correlated favorably with data.

Language: Английский

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Wastewater surveillance of SARS-CoV-2 and influenza in preK-12 schools shows school, community, and citywide infections

Water Research, Journal Year: 2023, Volume and Issue: 231, P. 119648 - 119648

Published: Jan. 20, 2023

Language: Английский

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Human viral nucleic acids concentrations in wastewater solids from Central and Coastal California USA

Scientific Data, Journal Year: 2023, Volume and Issue: 10(1)

Published: June 22, 2023

Abstract We measured concentrations of SARS-CoV-2, influenza A and B virus, respiratory syncytial virus (RSV), mpox human metapneumovirus, norovirus GII, pepper mild mottle nucleic acids in wastewater solids at twelve treatment plants Central California, USA. Measurements were made daily for up to two years, depending on the plant. using digital droplet (reverse-transcription–) polymerase chain reaction (RT-PCR) following best practices making environmental molecular biology measurements. These data can be used better understand disease occurrence communities contributing wastewater.

Language: Английский

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Impact of the COVID-19 pandemic on the prevalence of influenza A and respiratory syncytial viruses elucidated by wastewater-based epidemiology

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 880, P. 162694 - 162694

Published: March 8, 2023

Since the COVID-19 pandemic, a decrease in prevalence of Influenza A virus (IAV) and respiratory syncytial (RSV) has been suggested by clinical surveillance. However, there may be potential biases obtaining an accurate overview infectious diseases community. To elucidate impact on IAV RSV, we quantified RSV RNA wastewater collected from three treatment plants (WWTPs) Sapporo, Japan, between October 2018 January 2023, using highly sensitive EPISENS™ method. From to April 2020, M gene concentrations were positively correlated with confirmed cases corresponding area (Spearman's r = 0.61). Subtype-specific HA genes also detected, their showed trends that consistent clinically reported cases. B serotypes detected wastewater, 0.36-0.52). The detection ratios decreased 66.7 % (22/33) 42.4 (14/33) 4.56 (12/263) 32.7 (86/263), respectively city after prevalence. present study demonstrates usefulness wastewater-based epidemiology combined preservation (wastewater banking) as tool for better management viral diseases.

Language: Английский

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