Exposure to 6-PPD Quinone at Environmentally Relevant Concentrations Inhibits Both Lifespan and Healthspan in C. elegans

Environmental Science & Technology, Journal Year: 2023, Volume and Issue: 57(48), P. 19295 - 19303

Published: Nov. 8, 2023

N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6-PPD), one of the most common additives used in rubber, enters environment due to significant emissions tire wear particles. 6-PPD quinone (6-PPDQ) is an important derivative after ozonization. With concentrations ranging from nanograms per liter μg/L, 6-PPDQ has so far been identified a series water samples. Acute lethality coho salmon (LC50 < 1 μg/L) was lower than environmental 6-PPDQ, highlighting exposure risks 6-PPDQ. It becoming increasingly necessary investigate potential toxicity at concentrations. Here, we examined effect on lifespan and healthspan underlying mechanism Caenorhabditis elegans. Exposure (1 10 shortened lifespan. Meanwhile, during aging process, (0.1–10 could decrease both pumping rate locomotion behavior, suggesting healthspan. For molecular mechanism, dysregulation insulin signaling pathway linked In pathway, DAF-2 restricted function DAF-16 activate downstream targets (SOD-3 HSP-6), which turn controlled Additionally, response toxicity, peptides (INS-6, INS-7, DAF-28) corresponding receptor DAF-2. Therefore, environmentally relevant potentially causes damage by activating organisms.

Language: Английский

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Widespread occurrence of two typical N, N’-substituted p-phenylenediamines and their quinones in humans: Association with oxidative stress and liver damage

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 468, P. 133835 - 133835

Published: Feb. 19, 2024

Language: Английский

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Occurrence and partitioning of p-phenylenediamine antioxidants and their quinone derivatives in water and sediment

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 914, P. 170046 - 170046

Published: Jan. 11, 2024

Language: Английский

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Occurrence of p-phenylenediamine antioxidants in human urine

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 914, P. 170045 - 170045

Published: Jan. 12, 2024

Language: Английский

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Evaluation of 6-PPD quinone toxicity on lung of male BALB/c mice by quantitative proteomics

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 922, P. 171220 - 171220

Published: Feb. 25, 2024

Language: Английский

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Environmental profiles, hazard identification, and toxicological hallmarks of emerging tire rubber-related contaminants 6PPD and 6PPD-quinone

Environment International, Journal Year: 2024, Volume and Issue: 187, P. 108677 - 108677

Published: April 21, 2024

N-(1,3-dimethylbutyl)-N'-phenyl-p-phenylenediamine (6PPD) is commonly used in rubber compounds as antioxidants to protect against degradation from heat, oxygen, and ozone exposure. This practice extends the lifespan of products, including tires, by preventing cracking, aging, deterioration. However, environmental consequences waste generated during product use, particularly formation 6PPD-quinone (6PPD-Q) through reaction 6PPD with ozone, have raised significant concerns due their detrimental effects on ecosystems. Extensive research has revealed widespread occurrence its derivate 6PPD-Q various compartments, air, water, soil. The emerging substance been shown pose acute mortality long-term hazards aquatic terrestrial organisms at concentrations below environmentally relevant levels. Studies demonstrated toxic a range organisms, zebrafish, nematodes, mammals. These include neurobehavioral changes, reproductive dysfunction, digestive damage exposure pathways. Mechanistic insights suggest that mitochondrial stress, DNA adduct formation, disruption lipid metabolism contribute toxicity induced 6PPD-Q. Recent findings human samples, such blood, urine, cerebrospinal fluid, underscore importance further public health toxicological implications these compounds. distribution, fate, biological effects, underlying mechanisms environment highlight urgent need for additional understand address impacts

Language: Английский

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Chronic exposure to tire rubber-derived contaminant 6PPD-quinone impairs sperm quality and induces the damage of reproductive capacity in male mice

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 470, P. 134165 - 134165

Published: March 28, 2024

Language: Английский

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Exposure to 6-PPD quinone causes ferroptosis activation associated with induction of reproductive toxicity in Caenorhabditis elegans

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 471, P. 134356 - 134356

Published: April 19, 2024

Language: Английский

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An overview of the key topics related to the study of tire particles and their chemical leachates: From problems to solutions

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 172, P. 117563 - 117563

Published: Jan. 30, 2024

The environmental contamination and biological impacts of tire particles their associated chemicals is great concern. Variously termed as wear (TWP), road (TRWP), end-of-life tires (ELTs) to differentiate between possible compositions sources in the environment, these rubber are a major source plastic pollution. array added that leach into environment presents an additional environmental, toxicological regulatory challenge. In this review, we define several topic areas relevant study particles, outlining current state ongoing challenges. Emissions estimates needed establish loads based on dated, summarised dataset rather than measured data, but determining presence hampered by lack standardised sampling methods. Several techniques for analysis available, variability model composition makes selection analytical markers challenging, does commercial standards. Toxicologically, there remains need delineate particle leachate effects and, addition, investigate emerging concern transformation products. Such knowledge required feed processes which currently regulate constituents separately whole tire. Technological solutions capture or replace progressing, often outside academia, it important scientific community collaborate with those attempting reduce impact tires, including industry.

Language: Английский

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In Vitro and In Vivo Biotransformation Profiling of 6PPD-Quinone toward Their Detection in Human Urine

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(21), P. 9113 - 9124

Published: May 14, 2024

The antioxidant N-(1,3-Dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD) and its oxidized quinone product 6PPD-quinone (6PPD-Q) in rubber have attracted attention due to the ecological risk that they pose. Both 6PPD 6PPD-Q been detected various environments humans cohabit. However, date, a clear understanding of biotransformation potential biomarker for exposure are lacking. To address this issue, study presents comprehensive analysis extensive across species, encompassing both vitro vivo models. We tentatively identified 17 metabolites vitro, 15 mice vivo, confirmed presence two human urine samples. Interestingly, different patterns were observed species. Through semiquantitative based on peak areas, we found almost all underwent within 24 h mice, primarily via hydroxylation subsequent glucuronidation. This suggests rapid metabolic processing mammals, underscoring importance identifying effective biomarkers exposure. Notably, monohydroxy 6PPD-Q-O-glucuronide consistently most predominant our studies, highlighting as key epidemiological research. These findings represent first data set mammalian systems, offering insights into pathways involved possible biomarkers.

Language: Английский

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