Myofilaments promote wing expansion and maintain genitalia morphology in the American cockroach, Periplaneta americana DOI

Fangfang Liu,

Yingying Cui,

Huna Lu

et al.

Insect Molecular Biology, Journal Year: 2022, Volume and Issue: 32(1), P. 46 - 55

Published: Oct. 10, 2022

Abstract Insects are the most widely distributed and successful animals on planet. A large number of insects capable flight with functional wings. Wing expansion is an important process for to achieve wings after eclosion healthy genital morphology crucial adult reproduction. Myofilaments units that constitute sarcomeres trigger muscle contraction. Here, we identified four myofilament proteins, including Myosin, Paramyosin, Tropomyosin Troponin T, from wing pads nymphs in American cockroach, Periplaneta americana . RNAi‐mediated knockdown Myosin , Paramyosin T early stage final instar caused a severely curly phenotype imaginal moult, especially groups, indicating these proteins involved controlling behaviours during nymph‐adult transition. In addition, resulted abnormal external genitalia, ovulation failure, affected male accessory gland development. Interestingly, expression genes was induced by methoprene, juvenile hormone (JH) analogue, decreased depletion JH receptor gene Met Altogether, have determined play role promoting maintaining genitalia morphology, their signalling. Our data reveal novel mechanism which regulated myofilaments functions morphology.

Language: Английский

Peptidomics DOI
Roland Hellinger, Arnar Sigurdsson, Wenxin Wu

et al.

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: March 30, 2023

Language: Английский

Citations

57
NeuroPred-FRL: an interpretable prediction model for identifying neuropeptide using feature representation learning DOI
Md Mehedi Hasan, Md. Ashad Alam, Watshara Shoombuatong

et al.

Briefings in Bioinformatics, Journal Year: 2021, Volume and Issue: 22(6)

Published: April 10, 2021

Abstract Neuropeptides (NPs) are the most versatile neurotransmitters in immune systems that regulate various central anxious hormones. An efficient and effective bioinformatics tool for rapid accurate large-scale identification of NPs is critical immunoinformatics, which indispensable basic research drug development. Although a few NP prediction tools have been developed, it mandatory to improve their NPs’ performances. In this study, we developed machine learning-based meta-predictor called NeuroPred-FRL by employing feature representation learning approach. First, generated 66 optimal baseline models 11 different encodings, six classifiers two-step selection The predicted probability scores based on were combined be deemed as input vector. Second, order enhance ability, applied approach optimize 66-D vector then inputted one into random forest classifier final meta-model (NeuroPred-FRL) construction. Benchmarking experiments both cross-validation independent tests indicate achieves superior performance compared with other state-of-the-art predictors. We believe proposed can serve powerful NPs, facilitating characterization functional mechanisms expediting applications clinical therapy. Moreover, interpreted some model leveraging robust SHapley Additive exPlanation algorithm.

Language: Английский

Citations

78
Transcriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nodus DOI Creative Commons
Jens Habenstein, Franziska Schmitt, Sander Liessem

et al.

Journal of Neurochemistry, Journal Year: 2021, Volume and Issue: 158(2), P. 391 - 412

Published: March 11, 2021

Behavioral flexibility is an important cornerstone for the ecological success of animals. Social Cataglyphis nodus ants with their age-related polyethism characterized by behavioral phenotypes represent a prime example flexibility. We propose neuropeptides as powerful candidates flexible modulation transitions in individual ants. As neuropeptidome C. was unknown, we collected comprehensive peptidomic data set obtained transcriptome analysis ants' central nervous system combined brain extract Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling different regions matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. In total, identified 71 peptides likely bioactive function, encoded on 49 neuropeptide-, neuropeptide-like, protein hormone prepropeptide genes, including novel neuropeptide-like gene (fliktin). next spatial distribution subset 16 precursor proteins high resolution MALDI MS imaging (MALDI MSI) 14 µm sections. The accuracy our MSI were confirmed matching immunostaining patterns tachykinins ion images from consecutive Our provide solid framework future research into spatially resolved qualitative quantitative changes associated stage-specific functional role

Language: Английский

Citations

33
Effect of Periplaneta americana Residue Feed on Immunity, Antioxidant Capacity, and Transcriptome in Chickens: A Study on Sanhuang Chickens DOI Creative Commons

Yongfei Zhao,

Tianzhang Zhao,

Shunyi Zi

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(1), P. 94 - 94

Published: Jan. 3, 2025

This study investigated the effects of Periplaneta americana residue (PAR) on immune function, antioxidant capacity, and transcriptome Sanhuang chickens. Six hundred 30-day-old chickens were divided into six groups with diets varying PAR replacement soybean meal from 0 to 100% in increments 20%. Samples taken day 100. The results indicated that did not significantly affect growth performance (p > 0.05). elevated levels IL-1β, IL-2, TNF-α, IgA, IgG, IgM both serum spleen < Moreover, it markedly increased GSH-Px, T-AOC, SOD, CAT liver 0.05) reached optimal factors indicators at diet 2. Transcriptomic analysis revealed substituting for downregulated genes associated diseases infectious disease pathways. Substituting enhanced resistance pathogenic oxidative stress, no impact performance. improvement was observed

Language: Английский

Citations

0
Neuropeptides from a praying mantis: what the loss of pyrokinins and tryptopyrokinins suggests about the endocrine functions of these peptides DOI Creative Commons
Jan A. Veenstra

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e19036 - e19036

Published: Feb. 27, 2025

Neuropeptides play important roles in insects, but many cases their functions remain obscure. Comparative neuropeptidome analyses may provide clues to what these might be. Praying mantises are predators and close relatives of cockroaches that scavengers. Cockroach neuropeptidomes well established, little is known about mantis neuropeptides. The recently published genome assembly the praying Tenodera sinensis makes it Possible change that. from T. was analyzed for presence genes coding For comparison, publicly available short read archives this other species were also examined expression As a rule, Mantodea Blattodea almost identical; use very similar However, there one surprising exception. lack receptors pyrokinins, including those tryptopyrokinins. No typical pyrokinin found, some do have tryptopyrokinin gene, others has been lost and, speudogene. most no information where expressed, Deroplatys truncata thorax thus not suboesophageal ganglion, as insects. In genomic two species-out 52-sequences found specific receptor. phylogenetic position implies receptor gene independently on multiple occasions. loss happened more than once. independent losses suggests irrelevant mantises. This surprising, since strongly conserved neuroendocrine cells suboeosphageal ganglion. which known, its salivary gland. neuroendocrine, unlikely acutely regulate salivation, insects regulated by characterized neurons. If action prime gland subsequent would make perfect sense lose capacity, they can anticipate when will catch next prey. Priming days before actually needed be energetically costly. pyrokinins facilitate feeding fashion muscles moving food source digesting it. hypothesis provides good explanation why need function ubiquitous arthropod neuropeptides

Language: Английский

Citations

0
Neuropeptides in Rhipicephalus microplus and other hard ticks DOI Creative Commons
Jéssica Waldman, Marina Amaral Xavier, Larissa Rezende Vieira

et al.

Ticks and Tick-borne Diseases, Journal Year: 2022, Volume and Issue: 13(3), P. 101910 - 101910

Published: Jan. 31, 2022

Language: Английский

Citations

18
"Computational Approaches for Identifying Neuropeptides: A Comprehensive Review" DOI Creative Commons

Roger Rahmani,

Leila R Kalankesh, Reza Ferdousi

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 36(1), P. 102409 - 102409

Published: Nov. 29, 2024

Language: Английский

Citations

3
Different neuroendocrine cell types in the pars intercerebralis of Periplaneta americana produce their own specific IGF-related peptides DOI Creative Commons
Jan A. Veenstra

General and Comparative Endocrinology, Journal Year: 2023, Volume and Issue: 335, P. 114233 - 114233

Published: Feb. 13, 2023

Language: Английский

Citations

8
Differential expression of some termite neuropeptides and insulin/IGF-related hormones and their plausible functions in growth, reproduction and caste determination DOI Creative Commons
Jan A. Veenstra

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e15259 - e15259

Published: April 26, 2023

Background Insulin-like growth factor (IGF) and other insulin-like peptides (ilps) are important hormones regulating development in animals. Whereas most animals have a single female male adult phenotype, some insect species the same genome may lead to different final forms. Perhaps best known example is honeybee where females can either develop into queens or workers. More extreme forms of such polyphenism occur termites, queens, kings, workers soldiers coexist. Both juvenile hormone regulate reproduction as well polyphenism. In termites role induction soldier caste known, but IGF ilps these processes remains largely unknown. Here various termite identified hypotheses regarding their functions suggested. Methods Genome assemblies transcriptome short read archives (SRAs) were used identify neuropeptides determine expression species, tissues castes. Results Discussion Termites seven ilps, i.e. gonadulin, an ortholog Drosophila peptide 7 (dilp7), which commonly present insects, four smaller peptides, that collectively been called IGF-related (sirps) individually atirpin, birpin, cirpin brovirpin. Gonadulin lost from higher however amplified brovirpin gene, they often two three paralogs. Based on differential genes it seems likely atirpin autocrine tissue released when faces metabolic stress. Birpin be responsible for absence this reproductive adults or, present, soldiers. Brovirpin expressed both by brain ovary stimulates vitellogenesis, while function less clear.

Language: Английский

Citations

7
Comparative analysis of adipokinetic hormones and their receptors in Blattodea reveals novel patterns of gene evolution DOI Creative Commons
Shixiong Jiang, Heather G. Marco,

Nina Scheich

et al.

Insect Molecular Biology, Journal Year: 2023, Volume and Issue: 32(6), P. 615 - 633

Published: June 29, 2023

Adipokinetic hormone (AKH) is a neuropeptide produced in the insect corpora cardiaca that plays an essential role mobilising carbohydrates and lipids from fat body to haemolymph. AKH acts by binding rhodopsin-like G protein-coupled receptor (GPCR), adipokinetic (AKHR). In this study, we tackle ligand gene evolution as well evolutionary origins of paralogues order Blattodea (termites cockroaches). Phylogenetic analyses precursor sequences point ancient duplication event common ancestor Blaberoidea, yielding new group putative decapeptides. total, 16 different peptides 90 species were obtained. Two octapeptides seven putatively novel decapeptides are predicted for first time. 18 species, spanning solitary cockroaches subsocial wood roaches lower higher termites, subsequently acquired using classical molecular methods silico approaches employing transcriptomic data. Aligned AKHR open reading frames revealed 7 highly conserved transmembrane regions, typical arrangement GPCRs. based on support accepted relationships among termite, (Cryptocercus spp.) cockroach lineages large extent, while post-translational modification sites do not greatly differ between social termites. Our study provides important information only functional research but also further interested their development potential candidates biorational pest control agents against invasive termites cockroaches.

Language: Английский

Citations

7