Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Aug. 23, 2023

Safe and efficient antibacterial materials are urgently needed to combat drug-resistant bacteria biofilm-associated infections. The rational design of nanoparticles for flexible elimination biofilms remains challenging. Herein, we propose the fabrication Janus-structured targeting extracellular polymeric substance achieve dispersion or near-infrared (NIR) light-activated photothermal biofilms, respectively. Asymmetrical dextran-bismuth selenide (Dex-BSe) fabricated exploit synergistic effects both components. Interestingly, Janus Dex-BSe realize enhanced dispersal over time. Alternatively, taking advantage preferential accumulation at infection sites, self-propelled active motion induced by unique structure enhances killing effect. application biofilm removal NIR-triggered eradication in vivo is demonstrated Staphylococcus aureus-infected mouse excisional wound model abscess model, developed nanoplatform holds great promise diverse scenarios.

Language: Английский

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Molecularly self‐fueled nano-penetrator for nonpharmaceutical treatment of thrombosis and ischemic stroke

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 17, 2023

Thrombotic cerebro-cardiovascular diseases are the leading causes of disability and death worldwide. However, current drug therapeutics compromised by narrow therapeutic windows, unsatisfactory thrombolysis effects, severe bleeding events, high recurrence rates. In this study, we exploit a self-propelling nano-penetrator with fuel loading controllable motion features, which is molecularly co-assembled using photothermal photosensitizer (DiR) photothermal-activable NO donor (BNN6). The precisely engineered BNN6-DiR pair shows distinct advantages in terms productivity autonomous under laser irradiation. animal models artery/vein thrombosis acute ischemic stroke, self-fueled enables self-navigated thrombus-homing accumulation, self-propelled clot deep penetration, fluorescence image-guided photothermal/mechanical thrombolysis, NO-mediated prevention stroke salvage. As expected, displayed favorable outcomes without risk compared to clinically available thrombolytic drug. This study offers facile, safe, effective nonpharmaceutical modality towards clinical treatment stroke.

Language: Английский

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Adding Flying Wings: Butterfly-Shaped NIR-II AIEgens with Multiple Molecular Rotors for Photothermal Combating of Bacterial Biofilms

Journal of the American Chemical Society, Journal Year: 2023, Volume and Issue: 145(47), P. 25705 - 25715

Published: Nov. 16, 2023

The ever-increasing threats of multidrug-resistant bacteria and their biofilm-associated infections have bred a desperate demand for alternative remedies to combat them. Near-infrared (NIR)-absorbing photothermal agent (PTAs)-mediated therapy (PTT) is particularly attractive biofilm ablation thanks its superiorities noninvasive intervention, satisfactory antibacterial efficiency, less likelihood develop resistance. Herein, three butterfly-shaped aggregation-induced emission luminogens (AIEgens) with balanced nonradiative decay (for conducting PTT) radiative supplying fluorescence in the NIR-II optical window) are rationally designed imaging-assisted obliteration bacterial biofilms. After being encapsulated into cationic liposomes, AIEgens-fabricated nanoparticles can eradicate wide spectrum biofilms formed by Gram-positive (methicillin-resistant Staphylococcus aureus Enterococcus faecalis) Gram-negative (Escherichia coli Pseudomonas aeruginosa) upon an 808 nm laser irradiation. In vivo experiments firmly demonstrate that AIE liposomes excellent biocompatibility perform well both P. aeruginosa biofilm-induced keratitis mouse model MSRA skin infection model.

Language: Английский

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Local Antibiotic Delivery Options in Prosthetic Joint Infection

Antibiotics, Journal Year: 2023, Volume and Issue: 12(4), P. 752 - 752

Published: April 14, 2023

Prosthetic Joint Infection (PJI) causes significant morbidity and mortality for patients globally. Delivery of antibiotics to the site infection has potential improve treatment outcomes enhance biofilm eradication. These can be delivered using an intra-articular catheter or combined with a carrier substance pharmacokinetic properties. Carrier options include non-resorbable polymethylmethacrylate (PMMA) bone cement resorbable calcium sulphate, hydroxyapatite, bioactive glass, hydrogels. PMMA allows creation structural spacers used in multi-stage revision procedures, however it requires subsequent removal antibiotic compatibility levels are variable. Calcium sulphate is most researched PJI, but associated wound leakage hypercalcaemia, clinical evidence its effectiveness remains at early stage. Hydrogels provide versatile combability adjustable elution profiles, usage currently limited. Novel anti-biofilm therapies bacteriophages which have been successfully small case series.

Language: Английский

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Type I photodynamic antimicrobial therapy: Principles, progress, and future perspectives

Acta Biomaterialia, Journal Year: 2024, Volume and Issue: 177, P. 1 - 19

Published: Feb. 7, 2024

Language: Английский

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Chemical multiscale robotics for bacterial biofilm treatment

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(5), P. 2284 - 2299

Published: Jan. 1, 2024

A biofilm constitutes a bacterial community encased in sticky matrix of extracellular polymeric substances. These intricate microbial communities adhere to various host surfaces such as hard and soft tissues well indwelling medical devices. aggregates form robust substances (EPSs), leading the majority human infections. Such infections tend exhibit high resistance treatment, often progressing into chronic states. The EPS protects bacteria from hostile environment prevents penetration antibacterial agents. Modern robots at nano, micro, millimeter scales are highly attractive candidates for biomedical applications due their diverse functionalities, navigating confined spaces targeted multitasking. In this tutorial review, we describe key milestones strategies developed removal eradication biofilms using different sizes shapes. It can be seen that useful effective tools treating biofilms, thus preventing persistent infections, loss costly implanted devices, additional costs associated with hospitalization therapies.

Language: Английский

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Strategies to Promote the Journey of Nanoparticles Against Biofilm‐Associated Infections

Small, Journal Year: 2024, Volume and Issue: 20(10)

Published: Jan. 4, 2024

Abstract Biofilm‐associated infections are one of the most challenging healthcare threats for humans, accounting 80% bacterial infections, leading to persistent and chronic infections. The conventional antibiotics still face their dilemma poor therapeutic effects due high tolerance resistance led by biofilm barriers. Nanotechnology‐based antimicrobials, nanoparticles (NPs), paid attention extensively considered as promising alternative. This review focuses on whole journey NPs against biofilm‐associated clarify it clearly, is divided into four processes in sequence 1) Targeting biofilms, 2) Penetrating barrier, 3) Attaching cells, 4) Translocating through cell envelope. Through outlining compositions properties biofilms bacteria recent advances present strategies each process comprehensively discussed combat well combined these with drug resistance, aiming guide rational design facilitate wide application

Language: Английский

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Na+ inserted metal-organic framework for rapid therapy of bacteria-infected osteomyelitis through microwave strengthened Fenton reaction and thermal effects

Nano Today, Journal Year: 2021, Volume and Issue: 37, P. 101090 - 101090

Published: Jan. 28, 2021

Language: Английский

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Dual‐Propelled Lanbiotic Based Janus Micromotors for Selective Inactivation of Bacterial Biofilms

Angewandte Chemie International Edition, Journal Year: 2020, Volume and Issue: 60(9), P. 4915 - 4924

Published: Nov. 20, 2020

Graphene oxide/PtNPs/Fe2 O3 "dual-propelled" catalytic and fuel-free rotary actuated magnetic Janus micromotors modified with the lanbiotic Nisin are used for highly selective capture/inactivation of gram-positive bacteria units biofilms. Specific interaction Lipid II unit Staphylococcus Aureus in connection enhanced micromotor movement generated fluid flow result a 2-fold increase capture/killing ability (both bubble propulsion modes) as compared free peptide static counterparts. The high stability along towing force allow efficient operation untreated raw media (tap water, juice serum) even blood flowing mode. selectivity approach is illustrated by dramatically lower gram-negative (Escherichia Coli). double-propulsion (catalytic or magnetic) mode biocompatibility holds considerable promise to design tailored lanbiotics that can response changes make resistant myriad clinical, environmental remediation food safety applications.

Language: Английский

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Gold–Platinum Nanodots with High-Peroxidase-like Activity and Photothermal Conversion Efficiency for Antibacterial Therapy

ACS Applied Materials & Interfaces, Journal Year: 2021, Volume and Issue: 13(31), P. 37535 - 37544

Published: July 29, 2021

Combined therapeutic strategies for bacterial infection have attracted worldwide attention owing to their faster and more effective therapy with fewer side effects compared monotherapy. In this work, gold–platinum nanodots (AuPtNDs) are simply quickly synthesized by a one-step method. They not only exhibit powerful peroxidase-like activity but also confer higher affinity hydrogen peroxide (H2O2), which is 3.4 times that of horseradish peroxidase. Under 808 nm laser irradiation, AuPtNDs excellent photothermal conversion efficiency (50.53%) strong stability. Excitingly, they can combat through the combination chemodynamic therapy. vitro antibacterial results show combined strategy has broad-spectrum property against both Escherichia coli (Gram negative, 97.1%) Staphylococcus aureus positive, 99.3%). Animal experiments further effectively promote healing wounds. addition, good biocompatibility low toxicity, hardly traceable in main organs mice, indicates be well excreted metabolism. These reveal application potential as simple magic multifunctional nanoparticle open up new applications clinical anti-infective near future.

Language: Английский

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