Mitochondrial temperature homeostasis resists external metabolic stresses DOI Creative Commons
Mügen Terzioglu,

Kristo Veeroja,

Toni Montonen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 24, 2023

ABSTRACT Based on studies with a fluorescent reporter dye, Mito Thermo Yellow, and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell-line was estimated be up 15 °C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis, adenine nucleotide transporter and, if an auxiliary pathway for electron transfer available via alternative oxidase, even respiratory poisons acting downstream OXPHOS complex I. We propose high is inescapable consequence biochemistry oxidative phosphorylation homeostatically as primary feature metabolism. IMPACT STATEMENT Mitochondria are hotter than their living cells. In response diverse re-adjusts this value whenever possible.

Language: Английский

Spotlight on Luminescence Thermometry: Basics, Challenges, and Cutting‐Edge Applications DOI
Carlos D. S. Brites, Riccardo Marin, Markus Suta

et al.

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(36)

Published: July 21, 2023

Abstract Luminescence (nano)thermometry is a remote sensing technique that relies on the temperature dependency of luminescence features (e.g., bandshape, peak energy or intensity, and excited state lifetimes risetimes) phosphor to measure temperature. This provides precise thermal readouts with superior spatial resolution in short acquisition times. Although thermometry just starting become more mature subject, it exhibits enormous potential several areas, e.g., optoelectronics, photonics, micro‐ nanofluidics, nanomedicine. work reviews latest trends field, including establishment comprehensive theoretical background standardized practices. The reliability, repeatability, reproducibility are also discussed, along use multiparametric analysis artificial‐intelligence algorithms enhance readouts. In addition, examples provided underscore challenges faces, alongside need for continuous search design new materials, experimental techniques, procedures improve competitiveness, accessibility, popularity technology

Language: Английский

Citations

191
Local Temperature Increments and Induced Cell Death in Intracellular Magnetic Hyperthermia DOI Creative Commons
Yuanyu Gu, Rafael Piñol, Raquel Moreno‐Loshuertos

et al.

ACS Nano, Journal Year: 2023, Volume and Issue: 17(7), P. 6822 - 6832

Published: March 20, 2023

The generation of temperature gradients on nanoparticles heated externally by a magnetic field is crucially important in hyperthermia therapy. But the intrinsic low heating power nanoparticles, at conditions allowed for human use, limitation that restricts general implementation technique. A promising alternative local intracellular hyperthermia, whereby cell death (by apoptosis, necroptosis, or other mechanisms) attained small amounts heat generated thermosensitive sites. However, few experiments conducted determination have found increments are much higher than theoretical predictions, thus supporting hypothesis. Reliable measurements needed to get an accurate picture and resolve discrepancy. In this paper, we report real-time variation γ-Fe2O3 nanoheaters using Sm3+/Eu3+ ratiometric luminescent thermometer located its surface during exposure external alternating field. We measure maximum 8 °C without any appreciable increase membrane. Even with fields whose frequency intensity still well within health safety limits, these sufficient produce but noticeable death, which enhanced considerably as increased level tolerated consequently demonstrating feasibility hyperthermia.

Language: Английский

Citations

42
Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals DOI Creative Commons
Anneliese M. M. Gest, Ayse Z. Sahan, Yanghao Zhong

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(22), P. 12573 - 12660

Published: Nov. 13, 2024

Cellular function is controlled through intricate networks of signals, which lead to the myriad pathways governing cell fate. Fluorescent biosensors have enabled study these signaling in living systems across temporal and spatial scales. Over years there has been an explosion number fluorescent biosensors, as they become available for numerous targets, utilized spectral space, suited various imaging techniques. To guide users this extensive biosensor landscape, we discuss critical aspects proteins consideration development, smart tagging strategies, historical recent types, grouped by target, with a focus on design applications sensors systems.

Language: Английский

Citations

7
Quantitative Imaging of Genetically Encoded Fluorescence Lifetime Biosensors DOI Creative Commons
Cong Quang Vu, Satoshi Arai

Biosensors, Journal Year: 2023, Volume and Issue: 13(10), P. 939 - 939

Published: Oct. 19, 2023

Genetically encoded fluorescence lifetime biosensors have emerged as powerful tools for quantitative imaging, enabling precise measurement of cellular metabolites, molecular interactions, and dynamic processes. This review provides an overview the principles, applications, advancements in imaging with genetically using microscopy (go-FLIM). We highlighted distinct advantages lifetime-based measurements, including independence from expression levels, excitation power, focus drift, resulting robust reliable measurements compared to intensity-based approaches. Specifically, we on two types go-FLIM, namely Förster resonance energy transfer (FRET)-FLIM single-fluorescent protein (FP)-based FLIM biosensors, discuss their unique characteristics benefits. serves a valuable resource researchers interested leveraging study interactions metabolism high precision accuracy.

Language: Английский

Citations

12
Mitochondrial temperature homeostasis resists external metabolic stresses DOI Creative Commons
Mügen Terzioglu,

Kristo Veeroja,

Toni Montonen

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 11, 2023

Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the genetically encoded gTEMP ratiometric temperature indicator targeted to mitochondria, of active mitochondria in four mammalian one insect cell line was estimated be up 15°C above that external environment which cells were exposed. High mitochondrial maintained face variety metabolic stresses, including substrate starvation or modification, decreased ATP demand due inhibition cytosolic protein synthesis, adenine nucleotide transporter and, if an auxiliary pathway for electron transfer available via alternative oxidase, even respiratory poisons acting downstream oxidative phosphorylation (OXPHOS) complex I. We propose high is inescapable consequence biochemistry OXPHOS homeostatically as primary feature metabolism.

Language: Английский

Citations

12
Dual lanthanide Complexes-Loaded ratiometric nanothermometer for elucidating intracellular variations of temperature and calcium transport DOI

Nana Yin,

Yahua Yu,

Xiaojuan Wang

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 490, P. 151645 - 151645

Published: May 1, 2024

Language: Английский

Citations

4
Mitochondrial heat production: the elephant in the lab… DOI Creative Commons
Pierre Rustin, Howard T. Jacobs, Mügen Terzioglu

et al.

Trends in Biochemical Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Calcium-induced upregulation of energy metabolism heats neurons during neural activity DOI Creative Commons

Jia-Yang Wu,

Yutaka Shindo, Kohji Hotta

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 708, P. 149799 - 149799

Published: March 17, 2024

Language: Английский

Citations

3
Extremely Sensitive Genetically Encoded Temperature Indicator Enabling Measurement at the Organelle Level DOI Creative Commons

Shun‐ichi Fukushima,

Tetsuichi Wazawa,

Kazunori Sugiura

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(8), P. 3889 - 3897

Published: July 23, 2024

Intracellular temperature is a fundamental parameter in biochemical reactions. Genetically encoded fluorescent indicators (GETIs) have been developed to visualize intracellular thermogenesis; however, the sensitivity or localization capability specific organelles should further improved clearly capture when and where changes at subcellular level occur. Here, we new GETI, gMELT, composed of donor acceptor subunits, which cyan yellow proteins, respectively, as Förster resonance energy transfer (FRET) pair were fused with temperature-sensitive domains. The subunits associated dissociated response changes, altering FRET efficiency. Consequently, gMELT functioned fluorescence ratiometric indicator. Untagged was expressed cytoplasm, whereas versions signals targeted endoplasmic reticulum (ER) mitochondria. All variations enabled more sensitive measurements cellular compartments than those previous GETIs. gMELTs, tagged ER mitochondrial targeting sequences, used detect thermogenesis stimulated chemically, method previously known induce thermogenesis. observed comparable reports, assuming that readout exclusively due variations. Furthermore, demonstrated how macromolecular crowding influences given this factor can subtly affect readout. Investigating accounting for factors such crowding, will enhance our understanding phenomena.

Language: Английский

Citations

3
Mitochondria: great balls of fire DOI Creative Commons
Howard T. Jacobs, Pierre Rustin, Paule Bénit

et al.

FEBS Journal, Journal Year: 2024, Volume and Issue: 291(24), P. 5327 - 5341

Published: Nov. 14, 2024

Recent experimental studies indicate that mitochondria in mammalian cells are maintained at temperatures of least 50 °C. While acknowledging the limitations current methods and their interpretation, we here consider ramifications this finding for cellular functions evolution. We whether as heat-producing organelles had a role origin eukaryotes emergence homeotherms. The homeostatic responses mitochondrial temperature to externally applied heat imply existence molecular heat-sensing system mitochondria. findings high innermost compartments mitochondria, those surface immediately surrounding cytosol remain be determined. ask some aspects dynamics motility could reflect changes supply demand heat, production factor diseases immunity.

Language: Английский

Citations

3