When nanozymes meet deoxyribonucleic acid: Understanding their interactions and biomedical diagnosis applications

Deleted Journal, Journal Year: 2024, Volume and Issue: 2(2)

Published: March 4, 2024

Abstract As emerging alternatives to natural enzymes, nanoscale materials featuring enzyme‐like catalytic behaviors (nanozymes) exhibit some attractive merits including robust activity, low cost, and easy‐to‐regulate performance. These have enabled them be intensively used in the biomedical field recent years. To remedy lack of selectivity most nanozymes, deoxyribonucleic acid (DNA) chains with specific recognition functions are utilized integrate nanozymes produce various nanozyme–DNA combinations via adsorption/desorption. In formed combinations, DNA component provides molecular/ionic role, nanozyme part offers response catalytically amplified signals, enabling detect analytes biomarkers selectively sensitively. highlight this interesting topic, here we made a critical review interactions between their applications biosensing disease diagnosis. First, strategies for conjugation onto surface were introduced briefly. Then, summarized detail, where flexible modulations activity by adsorption/desorption as well factors analyzed, potential impacts caused on characteristics pointed out. After that, typical DNA‐mediated modulation toxic ion sensing, health risk factor monitoring, diagnosis introduced. end, prospects combination presented, future challenges also discussed, attract more interest effort advance promising area.

Language: Английский

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Fluorescent Fingerprint Identification of Protein Structural Changes and Disease-Specific Amyloid Beta Aggregates Based on a Single-Nanozyme Sensor Array

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

The misfolding of amyloid β (Aβ) peptides into an aggregation state is a central hallmark the onset Alzheimer's disease (AD). However, conventional methods are mainly focused on detecting specific Aβ peptide, which makes it difficult to recognize multiple analytes with different topological features and unfolded states at same time. Here, we propose simple universal sensing strategy construct fluorescence sensor array by using single-nanozyme probe combined three fluorescent substrates as recognition units protein structural changes identify between assemblies. In this system, fingerprint-like patterns produced from nonspecific interactions proteins units. As result, can accurately 13 kinds their mixtures ratios. Moreover, discriminate against diverse conformational forms. Most importantly, successfully distinguishes species, even in artificial cerebrospinal fluid samples human serum samples. This work provides attractive reliable for predicting pathologically relevant clinical diagnosis AD.

Language: Английский

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Recent advances in colorimetric sensors based on nanozymes with peroxidase-like activity

The Analyst, Journal Year: 2022, Volume and Issue: 148(3), P. 487 - 506

Published: Nov. 30, 2022

The research progress and applications of colorimetric sensors based on nanozymes with peroxidase-like activity.

Language: Английский

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Signal Amplification Strategy Design in Nanozyme‐Based Biosensors for Highly Sensitive Detection of Trace Biomarkers

Small Methods, Journal Year: 2023, Volume and Issue: 7(11)

Published: Oct. 10, 2023

Abstract Nanozymes show great promise in enhancing disease biomarker sensing by leveraging their physicochemical properties and enzymatic activities. These qualities facilitate signal amplification matrix effects reduction, thus boosting performance. In this review, recent studies from the last five years, concentrating on detection improvement through nanozyme‐based biosensing are examined. This enhancement primarily involves modulations of size, morphology, doping, modification, electromagnetic mechanisms, electron conduction efficiency, surface plasmon resonance nanozymes for increased sensitivity. addition, a comprehensive description synthesis tuning strategies employed has been provided. includes detailed elucidation catalytic mechanisms alignment with fundamental principles enhanced technology, accompanied presentation quantitatively analyzed results. Moreover, diverse applications strip sensing, colorimetric electrochemical surface‐enhanced Raman scattering have outlined. Additionally, limitations, challenges, corresponding recommendations concerning application summarized. Furthermore, insights offered into future development outlook biosensing. review aims to serve not only as reference sensitivity biosensors but also catalyst exploring nanozyme broader

Language: Английский

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Intelligent Identification of Cerebrospinal Fluid for the Diagnosis of Parkinson’s Disease

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(6), P. 2534 - 2542

Published: Feb. 1, 2024

Cerebrospinal fluid (CSF) biomarkers are more sensitive than the Movement Disorder Society (MDS) criteria for detecting prodromal Parkinson's disease (PD). Early detection of PD provides best chance successful implementation disease-modifying treatments, making it crucial to effectively identify CSF extracted from patients or normal individuals. In this study, an intelligent sensor array was built by using three metal–organic frameworks (MOFs) that exhibited varying catalytic kinetics after reacting with potential protein markers. Machine learning algorithms were used process fingerprint response patterns, allowing qualitative and quantitative assessment proteins. The results robust capable discriminating between non-PD via detection. k-nearest neighbor regression algorithm predict MDS scores a minimum mean square error 38.88. MOF is expected promote due its ability multiple targets could be in conjunction other techniques diagnose sensitively selectively.

Language: Английский

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Enhanced “Electronic Tongue” for Dental Bacterial Discrimination and Elimination Based on a DNA-Encoded Nanozyme Sensor Array

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(9), P. 11228 - 11238

Published: Feb. 25, 2024

Bacterial infections are the second leading cause of death around world, especially those caused by delayed treatment and misdiagnosis. Therefore, rapid discrimination effective elimination multiple bacteria great importance for improving survival rate in clinic. Herein, a novel colorimetric sensor array bacterial is constructed using programmable DNA-encoded iron oxide nanoparticles (IONPs) as sensing elements. Utilizing differential interactions on IONPs, 11 kinds dental 6 proteins have been successfully identified linear discriminant analysis (LDA). Moreover, developed system also performs well quantitative determination individual identification mixtures. More importantly, practicability this strategy further verified precise differentiation blind artificial saliva samples. Furthermore, used efficiently killing bacteria, demonstrating potential clinical prophylaxis therapy.

Language: Английский

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Colorimetric Nanozyme Sensor Array Based on Metal Nanoparticle-Decorated CNTs for Quantification of Pesticides in Real Water and Soil Samples

ACS Sustainable Chemistry & Engineering, Journal Year: 2024, Volume and Issue: 12(2), P. 728 - 736

Published: Jan. 2, 2024

Pesticides have become a concern to the environment and human health, since they are frequently employed control pests regulate plant growth. Although array-based pattern recognition has been demonstrated be successful method for identifying variety of analytes, developing sensing components that possess desirable surface diversity remains difficult task. Herein, we fabricated metal nanoparticle-decorated CNT (MDC) nanozyme in presence cationic receptor produce diverse noncovalent interactions when various pesticides were adsorbed on MDCs, which resulted enhancement their peroxidase-mimicking activities with different degrees, utilized it as colorimetric sensor array pesticide identification. The designed MDC could successfully distinguish eight exhibited fingerprint-like sequences at concentration 10 μM or segregated CBZ, DTM, ISP having linear range varying from 1 8 quantified low 10.8, 28.8, 16.8 nM R2 values 0.983, 0.949, 0.977, respectively. Very interestingly, two similar structures (CBZ ISP) precisely discriminated by without any overlapping. Additionally, effective segregation spiked soil water samples cross-classification practicability array.

Language: Английский

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Plasmon-Stimulated Colorimetry Biosensor Array for the Identification of Multiple Metabolites

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(6), P. 6849 - 6858

Published: Jan. 31, 2024

Rationally designing highly catalytic and stable nanozymes for metabolite monitoring is of great importance because their huge potential in early disease diagnosis. Herein, a novel nanozyme based on hierarchically structured CuS/ZnS with efficient peroxidase (POD)-mimic capability was developed synthesized multiple determination recognition via the plasmon-stimulated biosensor array strategy. The designed can simultaneously harvest plasmon triggered hot electron–hole pairs generate photothermal properties, leading to sharply boosted POD-mimic under 808 nm laser irradiation. Interestingly, interaction diversity POD-like nanomaterials, unique inhibitory effect metabolites activity could be signal response as differentiation. Thus, utilizing TMB typical chromogenic substrate addition H2O2, colorimetric produce diverse fingerprints three vital metabolisms (cysteine (Cys), ascorbic acid (AA), glutathione (GSH)), which precisely identified by principal component analysis (PCA). Notably, distinct fingerprint single different levels mixtures also achieved detection limit 1 μM. Most importantly, cell lysis effectively discriminated assay, implying its clinical

Language: Английский

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Accelerated and Precise Identification of Antioxidants and Pesticides Using a Smartphone-Based Colorimetric Sensor Array

Talanta, Journal Year: 2024, Volume and Issue: 277, P. 126275 - 126275

Published: May 22, 2024

Language: Английский

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Iridium nanoparticles supported on polyaniline nanotubes for peroxidase mimicking towards total antioxidant capacity assay of fruits and vegetables

Food Chemistry, Journal Year: 2024, Volume and Issue: 445, P. 138732 - 138732

Published: Feb. 13, 2024

Language: Английский

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