Cell Sheets Formation Enhances Therapeutic Effects of Human Umbilical Cord Mesenchymal Stem Cells on Spinal Cord Injury DOI Creative Commons
Yulin Zhao, Zhixian Wu, Yuchen Zhou

et al.

CNS Neuroscience & Therapeutics, Journal Year: 2024, Volume and Issue: 30(12)

Published: Dec. 1, 2024

ABSTRACT Background In recent years, the utilization of stem cell therapy and sheet technology has emerged as a promising approach for addressing spinal cord injury (SCI). However, most appropriate type mechanism action remain unclear at this time. This study sought to develop an SCI rat model evaluate therapeutic effects human umbilical mesenchymal (hUC‐MSC) sheets in model. Furthermore, mechanisms underlying vascular repair effect hUC‐MSC following were investigated. Methods A temperature‐responsive culture method was employed preparation sheets. The extracellular matrix (ECM) produced by hUC‐MSCs serves two distinct yet interrelated purposes. Firstly, it acts biologically active scaffold transplanted cells, facilitating their attachment proliferation. Secondly, provides mechanical support bridges stumps, thereby restoration function. formation cavity within evaluated using Hematoxylin Eosin (H&E) staining method. Subsequently, endothelial cells cultivated with conditioned medium (CM) obtained from or pro‐angiogenic impact (MSC‐CM) (CS‐CM) through CCK‐8 assay, wound healing tube assay vitro context. development glial scars, blood vessels, neurons, axons assessed immunofluorescence staining. Results comparison hUC‐MSCs, demonstrated more pronounced capacity facilitate induce regeneration newborn neurons site, while also reducing scar significantly enhancing motor function rats. Notably, under identical conditions, been associated paracrine increase ability themselves secrete growth factors. During course experiment, observed that secretion uPAR among factors present MSC‐CM CS‐CM. finding subsequently corroborated subsequent experiments, wherein promote angiogenesis via PI3K/Akt signaling pathway. Conclusion creation not only enhances biological but effectively retains locally injury. Therefore, transplantation can maximize greatly formation, promoting axons. Additionally, research findings prove activate pathway enhance angiogenesis. transfer entire sheets, absence introduction additional exogenous synthetic biomaterials, further augment potential clinical application.

Language: Английский

Biomedical potential of nanozymes: Harnessing redox enzyme mimicry for theranostic applications DOI
Somaye Shahraki,

Esmaeil Vaziri,

Ali Akbar Saboury

et al.

Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 517, P. 215937 - 215937

Published: June 15, 2024

Language: Английский

Citations

30
Antioxidant Nanozyme‐Engineered Mesenchymal Stem Cells for In Vivo MRI Tracking and Synergistic Therapy of Myocardial Infarction DOI
Wenjun Le,

Zeyi Sun,

Tieyan Li

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: 34(23)

Published: Feb. 14, 2024

Abstract Cell therapy is a promising approach for myocardial infarction (MI) treatment. However, this strategy often restricted by the harsh microenvironment of MI, such as excess ROS, high oxidative stress, inflammation, etc., and thus decreases curative effect. Additionally, distribution, migration, homing transplanted stem cells ambiguous, which also becomes bottleneck clinical translation. To address these challenges, herein, versatile antioxidant nanozyme designed polymerically modifying dopamine (PDA) onto surface Mn 3 O 4 . The obtained acts an efficient SOD mimic, eliminating relieving reducing inflammation to improve MI microenvironment. Simultaneously, @PDA serves excellent MRI contrast agent tracking MSCs. Then, MSCs are engineered with via endocytosis form @PDA‐MSCs (Abbr. E‐MSCs), possess superior viability, migration ability compared normal Furthermore, E‐MSCs exhibit anti‐oxidant anti‐inflammatory activity, thereby enhancing therapeutic efficacy Finally, in vivo synergistic potential explored model mice. Overall, work provides combine activity imaging properties nanozymes simultaneously facilitate

Language: Английский

Citations

12
Nanozymes: Potential Therapies for Reactive Oxygen Species Overproduction and Inflammation in Ischemic Stroke and Traumatic Brain Injury DOI
Yunfan Yang, Zi-Xiang Li, Xiaochong Fan

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(26), P. 16450 - 16467

Published: June 19, 2024

Nanozymes, which can selectively scavenge reactive oxygen species (ROS), have recently emerged as promising candidates for treating ischemic stroke and traumatic brain injury (TBI) in preclinical models. ROS overproduction during the early phase of these diseases leads to oxidative damage, has been a major cause mortality worldwide. However, clinical application ROS-scavenging enzymes is limited by their short vivo half-life inability cross blood-brain barrier. mimic catalytic function natural enzymes, several advantages, including cost-effectiveness, high stability, easy storage. These advantages render them superior disease diagnosis therapeutic interventions. This review highlights recent advancements nanozyme applications TBI, emphasizing potential mitigate detrimental effect overproduction, inflammation, barrier compromise. Therefore, nanozymes represent treatment modality conditions future medical practices.

Language: Английский

Citations

12
Injectable and Photocurable Gene Scaffold Facilitates Efficient Repair of Spinal Cord Injury DOI
Yan Gao, Kaiyu Wang, Shan Wu

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 16(4), P. 4375 - 4394

Published: Jan. 8, 2024

RNA interference-based gene therapy has led to a strategy for spinal cord injury (SCI) therapy. However, there have been high requirements regarding the optimal delivery vector siRNA-based SCI Here, we developed an injectable and photocurable lipid nanoparticle GelMA (PLNG) hydrogel scaffold controlled dual siRNA at wound site. The prepared PLNG could efficiently protect retain bioactivity of nanocomplex. It facilitated sustainable release along with degradation in 7 days. After loading targeting phosphatase tensin homologue (PTEN) macrophage migration inhibitory factor (MIF) simultaneously, locally administered siRNAs/PLNG improved Basso mouse scale (BMS) score recovered ankle joint movement plantar stepping after treatment only three doses. We further proved that successfully regulated activities neurons, microglia, macrophages, thus promoting neuron axon regeneration remyelination. protein array results suggested increase expression growth factors decrease inflammatory regulate neuroinflammation create neural repair environment. Our system is potential candidate

Language: Английский

Citations

11
Functional biomaterials for modulating the dysfunctional pathological microenvironment of spinal cord injury DOI Creative Commons

Dezun Ma,

Changlong Fu,

Fenglu Li

et al.

Bioactive Materials, Journal Year: 2024, Volume and Issue: 39, P. 521 - 543

Published: May 30, 2024

Spinal cord injury (SCI) often results in irreversible loss of sensory and motor functions, most SCIs are incurable with current medical practice. One the hardest challenges treating SCI is development a dysfunctional pathological microenvironment, which mainly comprises excessive inflammation, deposition inhibitory molecules, neurotrophic factor deprivation, glial scar formation, imbalance vascular function. To overcome this challenge, implantation functional biomaterials at site has been regarded as potential treatment for modulating microenvironment to support axon regeneration, remyelination site, recovery after SCI. This review summarizes characteristics recent advances well technologies used modulate inflammatory regulate reshape revascularization microenvironment. Moreover, technological limitations, challenges, future prospects promote efficient repair also discussed. will aid further understanding

Language: Английский

Citations

10
Nanozymes meet hydrogels: Fabrication, progressive applications, and perspectives DOI

Manyan Qiu,

Chaoxin Man, Qianyu Zhao

et al.

Advances in Colloid and Interface Science, Journal Year: 2025, Volume and Issue: 338, P. 103404 - 103404

Published: Jan. 23, 2025

Language: Английский

Citations

1
Metal–Organic Frameworks for Nerve Repair and Neural Stem Cell Therapy DOI

Ling Meng,

Na Ren,

Mengwei Dong

et al.

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(3)

Published: Oct. 15, 2023

Abstract Recently, neurological disorders have led to a growing global burden of fatalities and disabilities. The limited capacity for natural self‐regeneration poses significant challenge in repairing nervous system injuries, which is closely related the complex microenvironment nonregenerative nature neurons. Metal–organic frameworks (MOFs), with their distinctive structure properties including high surface area, porosity, tunability, stimuli‐responsive behavior, biocompatibility, biodegradability, stand as an auspicious platform devising therapeutic strategies aiming at nerve regeneration repair. By taking advantage these characteristics, researchers opportunity explore innovative methods endogenous stimulation, magnetic response therapy, phototherapy, ultrasound drug delivery systems treatment diseases. Moreover, MOFs‐based stem cell therapy also developed inhibit neuroinflammation oxidative stress, promote axon growth, regulate differentiation, regeneration, finally restore function injured nerves. In this paper, preparation strategy biological characteristics MOFs, highlighting applications injuries treating neural cells presented. Finally, outlook on future development challenges field neuroscience concerning MOFs provided.

Language: Английский

Citations

13
Chemically programmed nanozyme with microenvironment remodeling for combinatorial treatment of osteoarthritis DOI

Jiang Guo,

Canfeng Li, Jianjing Lin

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 485, P. 149897 - 149897

Published: Feb. 21, 2024

Language: Английский

Citations

5
3D printing incorporating gold nanozymes with mesenchymal stem cell-derived hepatic spheroids for acute liver failure treatment DOI
Yuanyuan Jin, Jiabin Zhang,

Xiaodie Chen

et al.

Biomaterials, Journal Year: 2024, Volume and Issue: 315, P. 122895 - 122895

Published: Oct. 19, 2024

Language: Английский

Citations

4
The recent research progress in the application of the nanozyme-hydrogel composite system for drug delivery DOI Creative Commons
Haichang Li, Zhenghong Liu, Pu Zhang

et al.

Drug Delivery, Journal Year: 2024, Volume and Issue: 31(1)

Published: Oct. 25, 2024

Hydrogels, comprising 3D hydrophilic polymer networks, have emerged as promising biomaterial candidates for emulating the structure of biological tissues and delivering drugs through topical administration with good biocompatibility. Nanozymes can catalyze endogenous biomolecules, thereby initiating or inhibiting in vivo processes. A nanozyme-hydrogel composite inherits functions hydrogels nanozymes, where nanozyme serves catalytic core hydrogel forms structural scaffold. Moreover, concentrate nanozymes targeted lesions binding a specific group substrates, resulting pathological microenvironment remodeling drug-penetrating barrier impairment. The also shields to prevent burst release during production reduce related toxicity. Currently, application these composites has been extended antibacterial, anti-inflammatory, anticancer, tissue repair applications. In this review, we elucidate preparation methods composites, provide compelling evidence their advantages drug delivery comprehensive overview application.

Language: Английский

Citations

4