Optical Control of Invertebrate nAChR and Behaviors with Dithienylethene-Imidacloprid DOI
Qi Xu, Chao Zhang, Zhi Ping Xu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(29), P. 11048 - 11055

Published: July 13, 2023

Photopharmacology involving azobenzene or dithienylethene has changed established methods of studying receptor functions, allowing for increasing the spatiotemporal resolution. There are no photopharmacological tools available invertebrate nicotinic acetylcholine (nAChR). Here, we report a photochromic ligand, dithienylethene-imidacloprid (DitIMI), using photoswitch embedded in nAChR agonist imidacloprid. It was found that DitIMI displayed good photochromism and fluorescence switching behaviors upon irradiation with UV/vis light aqueous solution. We demonstrated open-DitIMI low spontaneous vitro vivo activity but can be photoisomerized to highly active closed-form. Surprisingly, photoswitchable showed large difference insecticidal between open closed forms, which against Aphis craccivora ring-closed isomers 355 times corresponding ring-open isomers. This photoisomerization further translated photomodulation neuron membrane potential behavioral responses living mosquito larvae American cockroaches. The photomanipulation nACh neurotransmission opens new avenues understanding inhibitory circuits intact animals.

Language: Английский

Advances and opportunities in the exciting world of azobenzenes DOI
Florica Adriana Jerca, Valentin Victor Jerca, Richard Hoogenboom

et al.

Nature Reviews Chemistry, Journal Year: 2021, Volume and Issue: 6(1), P. 51 - 69

Published: Nov. 18, 2021

Language: Английский

Citations

303
Biocompatible and Biodegradable Polymer Optical Fiber for Biomedical Application: A Review DOI Creative Commons
Yue Wang, Yu Huang, Hongyi Bai

et al.

Biosensors, Journal Year: 2021, Volume and Issue: 11(12), P. 472 - 472

Published: Nov. 23, 2021

This article discusses recent advances in biocompatible and biodegradable polymer optical fiber (POF) for medical applications. First, the POF material its properties are summarized. Then, several common fabrication methods thoroughly discussed. Following that, clinical applications of POFs discussed, including optogenetics, biosensing, drug delivery, neural recording. biomedical expanded specific functionalization or design. Different research necessitate use different equipment to achieve desired results. Finally, difficulty implanting flexible varies with flexibility. We present our a clear logical manner that will be useful researchers seeking broad perspective on proposed topic. Overall, content provides comprehensive overview POFs, previous breakthroughs, as well advancements. Biodegradable fibers have numerous applications, opening up new avenues biomedicine.

Language: Английский

Citations

75
Wireless multi-lateral optofluidic microsystems for real-time programmable optogenetics and photopharmacology DOI Creative Commons
Yixin Wu, Mingzheng Wu, Abraham Vázquez‐Guardado

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Sept. 22, 2022

Abstract In vivo optogenetics and photopharmacology are two techniques for controlling neuronal activity that have immense potential in neuroscience research. Their applications tether-free groups of animals been limited part due to tools availability. Here, we present a wireless, battery-free, programable multilateral optofluidic platform with user-selected modalities optogenetics, pharmacology photopharmacology. This system features mechanically compliant microfluidic electronic interconnects, capabilities dynamic control over the rates drug delivery real-time programmability, simultaneously up 256 separate devices single cage environment. Our behavioral experiments demonstrate motor behaviors grouped mice through co-located gene controlled photolysis caged glutamate. These systems may expand scope wireless study neural processing animal models.

Language: Английский

Citations

44
Micro/nanosystems for controllable drug delivery to the brain DOI Creative Commons

Mingzhen Tian,

Zhichao Ma, Guang‐Zhong Yang

et al.

The Innovation, Journal Year: 2023, Volume and Issue: 5(1), P. 100548 - 100548

Published: Nov. 27, 2023

•Micro/nanosystems show their potential to address the challenges of precise drug delivery brain.•Microfluidic platforms enable creation biomimetic in vitro brain models.•Micro/nano materials is emerging as a key player controllable delivery.•The minimally invasive fiberbot microsystem reduces procedure's invasiveness.•Image tracking micro/nanosystems allows for controlled therapeutic interventions. Drug crucial treatment central nervous system disorders. While significant progress has been made recent years, there are still major achieving brain. Unmet clinical needs arise from various factors, including transport, handling large doses, methods crossing biological barriers, use imaging guidance, and effective models analyzing delivery. Recent advances have shown promise addressing some these challenges. These include utilization microfluidic test validate process setting, development novel micro/nanocarriers loads across blood-brain barrier, implementation micro-intervention systems delivering drugs through intraparenchymal or peripheral routes. In this article, we present review latest developments We also delve into relevant diseases, conventional methods. addition, discuss future prospects robotic equipped with directed transportation, real-time image closed-loop control.

Language: Английский

Citations

34
In vivo photopharmacology with a caged mu opioid receptor agonist drives rapid changes in behavior DOI
Xiang Ma, Desiree A. Johnson, Xinyi Jenny He

et al.

Nature Methods, Journal Year: 2023, Volume and Issue: 20(5), P. 682 - 685

Published: March 27, 2023

Language: Английский

Citations

25
Photoswitchable Pseudoirreversible Butyrylcholinesterase Inhibitors Allow Optical Control of Inhibition in Vitro and Enable Restoration of Cognition in an Alzheimer’s Disease Mouse Model upon Irradiation DOI
Matthias Scheiner, Alexandra Sink, Matthias Hoffmann

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(7), P. 3279 - 3284

Published: Feb. 9, 2022

To develop tools to investigate the biological functions of butyrylcholinesterase (BChE) and mechanisms by which BChE affects Alzheimer's disease (AD), we synthesized several selective, nanomolar active, pseudoirreversible photoswitchable inhibitors. The compounds were able specifically influence different kinetic parameters inhibition process light. For one compound, a 10-fold difference in IC50-values (44.6 nM cis, 424 trans) vitro was translated an "all or nothing" response with complete recovery murine cognition-deficit AD model at dosages as low 0.3 mg/kg.

Language: Английский

Citations

35
A Lab-in-a-Fiber optofluidic device using droplet microfluidics and laser-induced fluorescence for virus detection DOI Creative Commons
Helen E. Parker, Sanghamitra Sengupta, Achar V. Harish

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: March 3, 2022

Abstract Microfluidics has emerged rapidly over the past 20 years and been investigated for a variety of applications from life sciences to environmental monitoring. Although continuous-flow microfluidics is ubiquitous, segmented-flow or droplet offers several attractive features. Droplets can be independently manipulated analyzed with very high throughput. Typically, carried out within planar networks microchannels, namely, microfluidic chips. We propose that fibers offer an interesting alternative format key advantages enhanced optical coupling. Herein, we demonstrate generation monodisperse droplets uniaxial optofluidic Lab-in-a-Fiber scheme. combine laser-induced fluorescence (LIF) detection achieved through development side-coupling fiber, which term periscope fiber. This arrangement provides stable compact alignment. Laser-induced sensitivity low limits rapid response time making it method in situ real-time measurements. use well-established fluorophore, fluorescein, characterize device determine $$\sim$$ ∼ 0.9 nL droplets. present characterization data range fluorescein concentrations, establishing limit (LOD) 10 nM fluorescein. Finally, show operates realistic relevant regime by detecting reverse-transcription loop-mediated isothermal amplification (RT-LAMP) products context COVID-19 diagnostics. The represents step towards point-of-care digital RT-LAMP platform.

Language: Английский

Citations

29
Structurally Aligned Multifunctional Neural Probe (SAMP) Using Forest‐Drawn CNT Sheet onto Thermally Drawn Polymer Fiber for Long‐Term In Vivo Operation DOI Creative Commons
Woojin Jeon, Jae Myeong Lee, Yeji Kim

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(27)

Published: March 29, 2024

Abstract Neural probe engineering is a dynamic field, driving innovation in neuroscience and addressing scientific medical demands. Recent advancements involve integrating nanomaterials to improve performance, aiming for sustained vivo functionality. However, challenges persist due size, stiffness, complexity, manufacturing intricacies. To address these issues, neural interface utilizing freestanding CNT‐sheets drawn from CNT‐forests integrated onto thermally functional polymer fibers proposed. This approach yields device with structural alignment, resulting exceptional electrical, mechanical, electrochemical properties while retaining biocompatibility prolonged periods of implantation. Structurally Aligned Multifunctional Probe (SAMP) employing forest‐drawn CNT sheets demonstrates capabilities recording, neurotransmitter detection, brain/spinal cord circuit manipulation via optogenetics, maintaining functionality over year post‐implantation. The straightforward fabrication method's versatility, coupled the device's reliability, underscores significance this technique next‐generation carbon‐based implants. Moreover, longevity multifunctionality position it as promising platform long‐term research.

Language: Английский

Citations

6
Optical control of targeted protein degradation DOI Creative Commons
Martin Reynders, Dirk Trauner

Cell chemical biology, Journal Year: 2021, Volume and Issue: 28(7), P. 969 - 986

Published: June 10, 2021

Molecular glues and proteolysis targeting chimeras (PROTACs) have emerged as small-molecule tools that selectively induce the degradation of a chosen protein shown therapeutic promise. Recently, several approaches employing light an additional stimulus to control induced been reported. Here, we analyze principles guiding design such systems, provide survey literature published date, discuss opportunities for further development. Light-responsive degraders enable precise temporal spatial levels, making them useful research but also potential candidates human precision medicine.

Language: Английский

Citations

40
Selective Photoswitchable Allosteric Agonist of a G Protein-Coupled Receptor DOI Creative Commons

Prashant Donthamsetti,

David B. Konrad, Belinda E. Hetzler

et al.

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(24), P. 8951 - 8956

Published: June 11, 2021

G protein-coupled receptors (GPCRs) are the most common targets of drug discovery. However, similarity between related GPCRs combined with complex spatiotemporal dynamics receptor activation in vivo has hindered development. Photopharmacology offers possibility using light to control location and timing action by incorporating a photoisomerizable azobenzene into GPCR ligand, enabling rapid reversible switching an inactive active configuration. Recent advances this area include (i) photoagonists photoantagonists that directly activity but nonselective because they bind conserved sites, (ii) photoallosteric modulators selectively nonconserved sites indirectly modulating response endogenous ligand. In study, we designed photoswitchable allosteric agonist site for selectivity activates on its own provide direct control. This work culminated development aBINA, Gi/o-coupled metabotropic glutamate 2 (mGluR2). aBINA is first example new class precision drugs other clinically important signaling proteins.

Language: Английский

Citations

39