Recent Progress in Flexible Microelectrode Arrays for Combined Electrophysiological and Electrochemical Sensing DOI Creative Commons

Umisha Siwakoti,

Steven A. Jones, Deepak Kumbhare

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 100 - 100

Published: Feb. 10, 2025

Understanding brain function requires advanced neural probes to monitor electrical and chemical signaling across multiple timescales regions. Microelectrode arrays (MEAs) are widely used record neurophysiological activity various depths regions, providing single-unit resolution for extended periods. Recent advancements in flexible MEAs, built on micrometer-thick polymer substrates, have improved integration with tissue by mimicking the brain's soft nature, reducing mechanical trauma inflammation. These flexible, subcellular-scale MEAs can stable signals months, making them ideal long-term studies. In addition recording, been functionalized electrochemical neurotransmitter detection. Electroactive neurotransmitters, such as dopamine, serotonin, adenosine, be directly measured via methods, particularly carbon-based surfaces. For non-electroactive neurotransmitters like acetylcholine, glutamate, γ-aminobutyric acid, alternative strategies, enzyme immobilization aptamer-based recognition, employed generate signals. This review highlights recent developments MEA fabrication functionalization achieve both electrophysiological recordings, minimizing sensor fowling damage when implanted long-term. It covers multi-time scale detection, development of conducting nanomaterial composite coatings enhance sensitivity, incorporation recognition carbon electrodes MEAs. Finally, it summarizes strategies acquire measurements from same device.

Language: Английский

Frontiers in electrochemical sensors for neurotransmitter detection: towards measuring neurotransmitters as chemical diagnostics for brain disorders DOI Creative Commons
Yangguang Ou, Anna Marie Buchanan, Colby E. Witt

et al.

Analytical Methods, Journal Year: 2019, Volume and Issue: 11(21), P. 2738 - 2755

Published: Jan. 1, 2019

It is extremely challenging to chemically diagnose disorders of the brain. There hence great interest in designing and optimizing tools for direct detection chemical biomarkers implicated neurological improve diagnosis treatment. Tools that are capable monitoring brain chemicals, neurotransmitters particular, need be biocompatible, perform with high spatiotemporal resolution, ensure selectivity sensitivity. Recent advances electrochemical methods addressing these criteria; resulting devices demonstrate promise vivo neurotransmitter detection. None currently used diagnostic purposes, however cutting-edge technologies promising more sensitive, selective, faster, less invasive measurements. Via this review we highlight significant technical studies, performed last 5 years, believe will facilitate development disorders.

Language: Английский

Citations

107
Sub-second Dopamine and Serotonin Signaling in Human Striatum during Perceptual Decision-Making DOI Creative Commons
Dan Bang, Kenneth T. Kishida, Terry Lohrenz

et al.

Neuron, Journal Year: 2020, Volume and Issue: 108(5), P. 999 - 1010.e6

Published: Oct. 12, 2020

Recent animal research indicates that dopamine and serotonin, neuromodulators traditionally linked to appetitive aversive processes, are also involved in sensory inference decisions based on such inference. We tested this hypothesis humans by monitoring sub-second striatal serotonin signaling during a visual motion discrimination task separates uncertainty from decision difficulty factorial design. Caudate nucleus recordings (n = 4) revealed multi-scale encoding: three participants, tracked uncertainty, and, one participant, both deviations expected trial transitions within our Putamen 1) supported cognition-action separation between caudate putamen—a sub-division unique primates—with tracking times. These first-of-their-kind observations the human brain reveal role for non-reward-based aspects of cognition action.

Language: Английский

Citations

106
Fluorescent Biosensors for Neurotransmission and Neuromodulation: Engineering and Applications DOI Creative Commons
Anna V. Leopold, Daria M. Shcherbakova, Vladislav V. Verkhusha

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2019, Volume and Issue: 13

Published: Oct. 23, 2019

Understanding how neuronal activity patterns in the brain correlate with complex behavior is one of primary goals modern neuroscience. Chemical transmission major way communication between neurons; however, traditional methods detection neurotransmitter and neuromodulator transients mammalian lack spatiotemporal precision. Modern fluorescent biosensors for neurotransmitters neuromodulators allow monitoring chemical vivo millisecond precision single cell resolution. Changes biosensor brightness occur upon binding can be detected using fiber photometry, stationary microscopy miniaturized head-mounted microscopes. Biosensors expressed animal adeno-associated viral vectors, their cell-specific expression achieved Cre-recombinase expressing animals. Although initially were represented by glutamate biosensors, nowadays GABA, acetylcholine, glycine, norepinephrine dopamine are available as well. In this review, we overview functioning principles existing intensiometric ratiometric provide brief insight into variety neurotransmitter-binding proteins from bacteria, plants eukaryotes including G-protein coupled receptors, which may serve scaffolds. We next describe a workflow development biosensors. then discuss advanced setups functional imaging awake freely moving conclude providing application examples studies single-neuron

Language: Английский

Citations

105
Electrochemical Detection of Neurotransmitters DOI Creative Commons
Saikat Banerjee,

Stephanie McCracken,

Md Faruk Hossain

et al.

Biosensors, Journal Year: 2020, Volume and Issue: 10(8), P. 101 - 101

Published: Aug. 18, 2020

Neurotransmitters are important chemical messengers in the nervous system that play a crucial role physiological and physical health. Abnormal levels of neurotransmitters have been correlated with physical, psychotic, neurodegenerative diseases such as Alzheimer's, Parkinson's, dementia, addiction, depression, schizophrenia. Although multiple neurotechnological approaches reported literature, detection monitoring brain remains challenge continues to garner significant attention. Neurotechnology provides high-throughput, well fast specific quantification target analytes brain, without negatively impacting implanted region is highly desired for complex intercommunication neurotransmitters. Therefore, it develop clinical assessment techniques sensitive reliable monitor modulate these screen diseases. This review focuses on summarizing current electrochemical measurement capable sensing high temporal resolution real time. Advanced neurotransmitter platforms integrate nanomaterials biorecognition elements explored.

Language: Английский

Citations

100
pMAT: An open-source software suite for the analysis of fiber photometry data DOI
Carissa A. Bruno, Christopher OʼBrien,

Svetlana Bryant

et al.

Pharmacology Biochemistry and Behavior, Journal Year: 2020, Volume and Issue: 201, P. 173093 - 173093

Published: Dec. 29, 2020

Language: Английский

Citations

93
Versatile Tools for Understanding Electrosynthetic Mechanisms DOI
Eric C.R. McKenzie, Seyyedamirhossein Hosseini, Ana G. Couto Petro

et al.

Chemical Reviews, Journal Year: 2021, Volume and Issue: 122(3), P. 3292 - 3335

Published: Dec. 17, 2021

Electrosynthesis is a popular, green alternative to traditional organic methods. Understanding the mechanisms not trivial yet necessary optimize reaction processes. To this end, multitude of analytical tools available identify and quantitate products intermediates. The first portion review serves as guide that underscores electrosynthesis fundamentals, including instrumentation, electrode selection, impacts electrolyte solvent, cell configuration, methods electrosynthesis. Next, broad base techniques aid in mechanism elucidation are covered detail. These divided into electrochemical, spectroscopic, chromatographic, microscopic, computational. Technique selection dependent on predicted pathways electrogenerated Often, combination must be utilized ensure accuracy proposed model. conclude, future prospects aim enhance field discussed.

Language: Английский

Citations

92
A Highly Sensitive A-Kinase Activity Reporter for Imaging Neuromodulatory Events in Awake Mice DOI Creative Commons
Lei Ma, Bart C. Jongbloets,

Wei-Hong Xiong

et al.

Neuron, Journal Year: 2018, Volume and Issue: 99(4), P. 665 - 679.e5

Published: Aug. 1, 2018

Language: Английский

Citations

84
Deep Learning for Voltammetric Sensing in a Living Animal Brain DOI
Yifei Xue, Wenliang Ji, Ying Jiang

et al.

Angewandte Chemie International Edition, Journal Year: 2021, Volume and Issue: 60(44), P. 23777 - 23783

Published: Aug. 19, 2021

Abstract Numerous neurochemicals have been implicated in the modulation of brain function, making them appealing analytes for sensors and diagnostics. However, it is a grand challenge to selectively measure multiple simultaneously vivo because their great variations concentrations, dynamic nature, composition. Herein, we present deep learning‐based voltammetric sensing platform highly selective simultaneous analysis three living animal brain. The system features carbon fiber electrode capable capturing mixed dynamics neurotransmitter, neuromodulator, ions. Then powerful neural network employed resolve individual chemical spatial‐temporal information. With this, single electrochemical measurement reveals an interplaying concentration changes dopamine, ascorbate, ions rat brain, which unobtainable with existing analytical methodologies. Our strategy provides means expedite research neuroscience empower sensing‐aided diagnostic applications.

Language: Английский

Citations

65
Significance of an Electrochemical Sensor and Nanocomposites: Toward the Electrocatalytic Detection of Neurotransmitters and Their Importance within the Physiological System DOI Creative Commons
Harjot Kaur, Samarjeet Singh Siwal, Reena V. Saini

et al.

ACS Nanoscience Au, Journal Year: 2022, Volume and Issue: 3(1), P. 1 - 27

Published: Oct. 28, 2022

A prominent neurotransmitter (NT), dopamine (DA), is a chemical messenger that transmits signals between one neuron to the next pass on signal and from central nervous system (CNS). The imbalanced concentration of DA may cause numerous neurological sicknesses syndromes, for example, Parkinson's disease (PD) schizophrenia. There are many types NTs in brain, including epinephrine, norepinephrine (NE), serotonin, glutamate. Electrochemical sensors have offered creative direction biomedical analysis testing. Researches progress improve performance develop new protocols sensor design. This review article focuses area growth discover applicability polymers metallic particles composite materials as tools electrochemical surface incorporation. attracted attention researchers they possess high sensitivity, quick reaction rate, good controllability, instantaneous detection. Efficient complex provide considerable benefits biological detection exclusive physical properties. Due distinctive electrocatalytic characteristics, nanoparticles add fascinating traits depend material's morphology size. Herein, we collected much information their importance within physiological system. Furthermore, corresponding techniques (such voltammetric, amperometry, impedance, chronoamperometry) different electrodes' roles discussed. other methods detecting include optical microdialysis methods. Finally, show advantages disadvantages conclude remarks with future perspectives.

Language: Английский

Citations

54
Fast‐Scanning Potential‐Gated Organic Electrochemical Transistors for Highly Sensitive Sensing of Dopamine in Living Rat Brain DOI

Weiqi Li,

Jing Jin, Tianyi Xiong

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(31)

Published: May 18, 2022

Developing techniques for the highly sensitive assay of neurotransmitters is essential understanding physiological and pathological processes. Here, we demonstrate a fast-scanning potential (FSP)-gated organic electrochemical transistor (OECT): sensing dopamine (DA) in living rat brain. The configuration combines selectivity fast-scan cyclic voltammetry (FSCV) with high sensitivity an OECT. combined use FSP as gating mode transconductance (gm ) parameter further improve performance terms sensitivity, limit detection, reproducibility, stability. FSP-OECT exhibits 0.899 S M-1 low detection down to 5 nM was validated vivo monitoring basal level electrically stimulated release DA.

Language: Английский

Citations

49