A Novel Conductive Polypyrrole‐Chitosan Hydrogel Containing Human Endometrial Mesenchymal Stem Cell‐Derived Exosomes Facilitated Sustained Release for Cardiac Repair

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(10)

Published: Jan. 4, 2024

Abstract Myocardial infarction (MI) results in cardiomyocyte necrosis and conductive system damage, leading to sudden cardiac death heart failure. Studies have shown that biomaterials can restore conduction, but cannot facilitate tissue regeneration. This study aims add regenerative capabilities the biomaterial by incorporating human endometrial mesenchymal stem cell (hEMSC)‐derived exosomes (hEMSC‐Exo) into poly‐pyrrole‐chitosan (PPY‐CHI), yield an injectable hydrogel effectively treat MI. In vitro, PPY‐CHI/hEMSC‐Exo, compared untreated controls, PPY‐CHI, or hEMSC‐Exo alone, alleviates H 2 O ‐induced apoptosis promotes tubule formation, while vivo, PPY‐CHI/hEMSC‐Exo improves post‐MI functioning, along with counteracting against ventricular remodeling fibrosis. All these activities are facilitated via increased epidermal growth factor (EGF)/phosphoinositide 3‐kinase (PI3K)/AKT signaling. Furthermore, properties of able resynchronize electrical transmission alleviate arrythmia. Overall, synergistically combines PPY‐CHI improve promoting angiogenesis inhibiting apoptosis, as well resynchronizing ultimately enable more effective MI treatment. Therefore, a provides dual benefits terms maintaining conductivity, facilitating long‐term exosome release sustained application their beneficial effects.

Language: Английский

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Understanding exosomes: Part 2—Emerging leaders in regenerative medicine

Periodontology 2000, Journal Year: 2024, Volume and Issue: 94(1), P. 257 - 414

Published: Feb. 1, 2024

Abstract Exosomes are the smallest subset of extracellular signaling vesicles secreted by most cells with ability to communicate other tissues and cell types over long distances. Their use in regenerative medicine has gained tremendous momentum recently due their be utilized as therapeutic options for a wide array diseases/conditions. Over 5000 publications currently being published yearly on this topic, number is only expected dramatically increase novel strategies continue developed. Today exosomes have been applied numerous contexts including neurodegenerative disorders (Alzheimer's disease, central nervous system, depression, multiple sclerosis, Parkinson's post‐traumatic stress disorders, traumatic brain injury, peripheral nerve injury), damaged organs (heart, kidney, liver, stroke, myocardial infarctions, ovaries), degenerative processes (atherosclerosis, diabetes, hematology musculoskeletal degeneration, osteoradionecrosis, respiratory disease), infectious diseases (COVID‐19, hepatitis), procedures (antiaging, bone regeneration, cartilage/joint osteoarthritis, cutaneous wounds, dental dermatology/skin erectile dysfunction, hair regrowth, intervertebral disc repair, spinal cord vascular regeneration), cancer therapy (breast, colorectal, gastric osteosarcomas), immune function (allergy, autoimmune regulation, inflammatory diseases, lupus, rheumatoid arthritis). This scoping review first its kind aimed at summarizing extensive potential broad range disorders.

Language: Английский

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Restoring Cardiac Functions after Myocardial Infarction–Ischemia/Reperfusion via an Exosome Anchoring Conductive Hydrogel

ACS Applied Materials & Interfaces, Journal Year: 2021, Volume and Issue: 13(48), P. 56892 - 56908

Published: Nov. 25, 2021

Both myocardial infarction (MI) and the follow-up reperfusion will lead to an inevitable injury tissues, such as cardiac dysfunctions, fibrosis, reduction of intercellular cell-to-cell interactions. Recently, exosomes (Exo) derived from stem cells have demonstrated a robust capability promote angiogenesis tissue repair. However, short half-life Exo rapid clearance insufficient therapeutic doses in lesion area. Herein, injectable conductive hydrogel is constructed bind human umbilical cord mesenchymal treat injuries after infarction-ischemia/reperfusion (MI-I/R). To this end, hyperbranched epoxy macromer (EHBPE) grafted by aniline tetramer (AT) was synthesized cross-link thiolated hyaluronic acid (HA-SH) anchoring CP05 peptide via epoxy/thiol "click" reaction. The resulting Gel@Exo composite system possesses multiple features, controllable gelation kinetics, shear-thinning injectability, conductivity matching native myocardium, soft dynamic stability adapting heartbeats, excellent cytocompatibility. After being injected into injured hearts rats, effectively prolongs retention ischemic myocardium. functions been considerably improved administration, indicated enhancing ejection fraction fractional shortening, reducing fibrosis Immunofluorescence staining reverse transcription-polymerase chain reaction (RT-PCR) results demonstrate that expression cardiac-related proteins (Cx43, Ki67, CD31, α-SMA) genes (VEGF-A, VEGF-B, vWF, TGF-β1, MMP-9, Serca2a) are remarkably upregulated. can significantly improve interactions, cell proliferation angiogenesis, result prominent effect on MI-I/R, providing promising method for tissues.

Language: Английский

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Extracellular vesicles as delivery systems at nano-/micro-scale

Advanced Drug Delivery Reviews, Journal Year: 2021, Volume and Issue: 179, P. 113910 - 113910

Published: Aug. 3, 2021

Language: Английский

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Constructing a cell microenvironment with biomaterial scaffolds for stem cell therapy

Stem Cell Research & Therapy, Journal Year: 2021, Volume and Issue: 12(1)

Published: Nov. 22, 2021

Stem cell therapy is widely recognized as a promising strategy for exerting therapeutic effects after injury in degenerative diseases. However, limitations such low retention and survival rates transplantation exist clinical applications. In recent years, emerging biomaterials that provide supportable cellular microenvironment transplanted cells have optimized the efficacy of stem injured tissues or organs. Advances engineered are revolutionizing our understanding cell-based therapies by co-transplanting with synthetic tissue-derived biomaterials, which offer scaffold propose an unprecedented opportunity to further employ significant influences tissue repair regeneration.

Language: Английский

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Chitosan hydrogel-loaded MSC-derived extracellular vesicles promote skin rejuvenation by ameliorating the senescence of dermal fibroblasts

Stem Cell Research & Therapy, Journal Year: 2021, Volume and Issue: 12(1)

Published: March 20, 2021

Abstract Background The senescence of dermal fibroblasts (DFLs) leads to an imbalance in the synthesis and degradation extracellular matrix (ECM) proteins, presenting so-called senescence-associated secretory phenotype (SASP), which ultimately skin aging. Recently, mesenchymal stem cell (MSC)-derived vesicles (EVs) have been recognized as a promising cell-free therapy for degenerative diseases, opens new avenue aging treatment. Methods In this study, we utilized chitosan (CS) hydrogel effective loading sustained release EVs. vitro, explored rejuvenation effects CS hydrogel-incorporated EVs (CS-EVs) on replicative DFLs through series experiments such β-galactosidase (SA-β-gal) staining, RT-PCR, Western blot analysis. Besides, employed local multi-site subcutaneous injection treat naturally aged mice with CS-EVs DiI fluorescent dye was used label achieve vivo real-time tracking. Results can significantly improve biological functions senescent fibroblasts, including promoting their proliferation, enhancing ECM inhibiting overexpression metalloproteinases (MMPs). Moreover, could prolong increase retention vivo. After treatment, tissues showed state, manifested explicitly enhanced expression collagen, decreased SASP-related factors, restoration tissue structures. Conclusions hydrogel-encapsulated delay processes by ameliorating function DFLs. Our results also highlight potential novel therapeutic strategy improving rejuvenation.

Language: Английский

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An injectable and conductive TEMPOL/polypyrrole integrated peptide co-assembly hydrogel promotes functional maturation of cardiomyocytes for myocardial infarction repair

Composites Part B Engineering, Journal Year: 2022, Volume and Issue: 236, P. 109794 - 109794

Published: March 11, 2022

Language: Английский

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A comprehensive review on peptide-bearing biomaterials: From ex situ to in situ self-assembly

Coordination Chemistry Reviews, Journal Year: 2023, Volume and Issue: 502, P. 215600 - 215600

Published: Dec. 14, 2023

Language: Английский

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Sustained Release of Neuroprotective Drugs Curcumin and Edaravone from Supramolecular Hydrogel for Ischemic Stroke Treatment

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(49)

Published: Aug. 2, 2023

Abstract Ischemic stroke is a major cause of death and disability worldwide. The poor drug delivery to cerebral ischemic regions remains challenging issue for treatment. Curcumin (Cur) edaravone (EDV) show remarkable therapeutic effects on stroke. However, the short half‐life aqueous solubility limit their application long‐term effective neuroprotection. Cur EDV with well‐studied hydrogelator Nap‐Phe‐Phe‐Tyr‐OH (NapFFY) co‐assembled prepare novel supramolecular peptide hydrogel EDV/Cur/NapFFY , which can improve bioavailability transport hydrophobic drugs sites precisely by local administration. In vitro release test demonstrate that co‐assembly NapFFY enables continuous about two weeks. Animal studies found effectively promote brain plasticity enhance functional recovery photothrombotic mouse model. Overall, work reveales great potential system in promoting repair

Language: Английский

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Production and Utility of Extracellular Vesicles with 3D Culture Methods

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(2), P. 663 - 663

Published: Feb. 16, 2023

In recent years, extracellular vesicles (EVs) have emerged as promising biomarkers, cell-free therapeutic agents, and drug delivery carriers. Despite their great clinical potential, poor yield unscalable production of EVs remain significant challenges. When using 3D culture methods, such scaffolds bioreactors, large numbers cells can be expanded the cell environment manipulated to control phenotype. This has been employed successfully increase well enhance effects. The physiological relevance cultures, spheroids, also provided a strategy for understanding role in pathogenesis several diseases evaluate tools deliver drugs. Additionally, methods encapsulate achieve more sustained effects prevent premature clearance enable localised concentrated exosome dosage. review highlights opportunities drawbacks different use EV research.

Language: Английский

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