TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 179, P. 117890 - 117890
Published: July 30, 2024
Language: Английский
Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 214, P. 114501 - 114501
Published: June 25, 2022
Language: Английский
Citations
60
Journal of Colloid and Interface Science, Journal Year: 2022, Volume and Issue: 613, P. 406 - 414
Published: Jan. 10, 2022
Language: Английский
Citations
48
Small Methods, Journal Year: 2022, Volume and Issue: 6(10)
Published: Sept. 16, 2022
Abstract Infectious pathogens cause severe human illnesses and great deaths per year worldwide. Rapid, sensitive, accurate detection of is importance for preventing infectious diseases caused by optimizing medical healthcare systems. Inspired a microbial defense system (i.e., CRISPR/ CRISPR‐associated proteins (Cas) system, an adaptive immune protecting microorganisms from being attacked invading species), many new biosensors have been successfully developed widely applied in the viruses pathogenic bacteria. Moreover, advanced nanotechnologies also integrated into these to improve their stability, sensitivity, accuracy. In this review, recent advance CRISPR/Cas systems‐based nano/biosensors applications bacteria are comprehensively reviewed. First all, categories working principles systems establishing simply introduced. Then, design construction discussed. end, attentions focused on Impressively, remaining opportunities challenges further development system‐based promising proposed.
Language: Английский
Citations
48
ACS Sensors, Journal Year: 2023, Volume and Issue: 8(3), P. 1076 - 1084
Published: Jan. 18, 2023
Next-generation biosensing tools based on CRISPR/Cas have revolutionized the molecular detection. A number of CRISPR/Cas-based biosensors been reported for detection nucleic acid targets. The establishment efficient methods non-nucleic target would further broaden scope this technique, but up to now, concerning research is limited. In current study, we a versatile platform small-molecule called SMART-Cas12a (small-molecule aptamer regulated test using CRISPR/Cas12a). Simply, hybridization chain reaction cascade signal amplification was first trigged by functional (aptamer) through binding. Then, system integrated recognize amplified products followed activation trans-cleavage. As such, can be ingeniously converted signals and then fluorescent that readily visualized analyzed customized 3D-printed visualizer with help home-made App-enabled smartphone. Adenosine triphosphate selected as model target, under optimized conditions, achieved fine analytical performance linear range from 0.1 750 μM limit 1.0 nM. satisfactory selectivity recoveries obtained demonstrated method suitable complex sample environment. sample-to-answer time less than 100 min. Our work not only expanded reach CRISPR-Cas in also provided prototype generalized detecting wider analytes desirable adaptability, sensitivity, specificity, on-site capability.
Language: Английский
Citations
43
Coordination Chemistry Reviews, Journal Year: 2022, Volume and Issue: 459, P. 214453 - 214453
Published: Feb. 14, 2022
Language: Английский
Citations
41
Small, Journal Year: 2023, Volume and Issue: 19(21)
Published: Feb. 25, 2023
Due to their superiority in the simple design and precise targeting, clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have attracted significant interest for biosensing. On one hand, CRISPR-Cas capacity precisely recognize cleave specific DNA RNA sequences. other such as orthologs of Cas9, Cas12, Cas13 exhibit cis-cleavage or trans-cleavage activities after recognizing target sequence. Owing cleavage activities, can be designed biosensing by degrading tagged nucleic acids produce detectable signals. To meet requirements point-of-care detection versatile signal readouts, gold nanomaterials with excellent properties high extinction coefficients, easy surface functionalization, biocompatibility are implemented CRISPR-Cas-based biosensors. In combination nanoparticles, nanorods, nanostars, great efforts devoted fabricating biosensors diverse targets. This review focuses on current advances nanomaterials-implemented biosensors, particularly working mechanism performance these systems, including CRISPR-Cas9, CRISPR-Cas12a, CRISPR-Cas13a discussed highlighted. Meanwhile, prospects challenges also strategies based systems.
Language: Английский
Citations
40
Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 219, P. 114827 - 114827
Published: Oct. 19, 2022
Language: Английский
Citations
39
Trends in Food Science & Technology, Journal Year: 2023, Volume and Issue: 135, P. 174 - 189
Published: April 3, 2023
Language: Английский
Citations
37
Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(37), P. 13577 - 13594
Published: Sept. 1, 2023
Non-nucleic acid targets have posed a serious challenge to food safety. The detection of non-nucleic can enable us monitor contamination in timely manner. In recent years, the CRISPR/Cas system has been extensively explored biosensing. However, there is lack summary CRISPR/Cas-powered tailored involved This review comprehensively summarizes advances on construction and promising applications field safety related targets. current challenges futuristic perspectives are also proposed accordingly. rapidly evolving provided powerful propellant for target via integration with aptamer and/or DNAzyme. Compared traditional analytical methods, conceptually novel, essentially eliminates dependence large instruments, demonstrates capability rapid, accurate, sensitive, on-site testing.
Language: Английский
Citations
30
Food Bioscience, Journal Year: 2023, Volume and Issue: 56, P. 103110 - 103110
Published: Sept. 9, 2023
Language: Английский
Citations
30