Diagnostic Modalities in Critical Care: Point-of-Care Approach DOI Creative Commons
Saša Rajšić, Robert Breitkopf, Mirjam Bachler

et al.

Diagnostics, Journal Year: 2021, Volume and Issue: 11(12), P. 2202 - 2202

Published: Nov. 25, 2021

The concept of intensive care units (ICU) has existed for almost 70 years, with outstanding development progress in the last decades. Multidisciplinary critically ill patients become an integral part every modern health system, ensuing improved and reduced mortality. Early recognition severe medical surgical illnesses, advanced prehospital organized immediate trauma centres led to a rise ICU patients. Due underlying disease its need complex mechanical support monitoring treatment, it is often necessary facilitate bed-side diagnostics. Immediate diagnostics are essential successful treatment life threatening conditions, early complications good quality care. Management incomprehensible without continuous sophisticated monitoring, bedside ultrasonography, diverse radiologic diagnostics, blood gas analysis, coagulation management, laboratory other point-of-care (POC) diagnostic modalities. Moreover, time acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic, particular attention given POC techniques due additional concerns related risk infection transmission, patient healthcare workers safety potential adverse events relocation. This review summarizes most actual information on possible modalities critical care, special focus importance approach imaging procedures.

Language: Английский

Recent Advances in Colorimetric Sensors Based on Gold Nanoparticles for Pathogen Detection DOI Creative Commons
Jianyu Yang, Xin Wang, Yuyang Sun

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 13(1), P. 29 - 29

Published: Dec. 26, 2022

Infectious pathogens cause severe threats to public health due their frightening infectivity and lethal capacity. Rapid accurate detection of is great significance for preventing infection. Gold nanoparticles have drawn considerable attention in colorimetric biosensing during the past decades unique physicochemical properties. Colorimetric diagnosis platforms based on functionalized AuNPs are emerging as a promising pathogen-analysis technique with merits high sensitivity, low-cost, easy operation. This review summarizes recent development this field. We first introduce detecting characteristics gold nanoparticles. Four types strategies, including application indirect target-mediated aggregation, chromogenic substrate-mediated catalytic activity, point-of-care testing (POCT) devices, machine learning-assisted sensor arrays, systematically introduced. In particular, three biomolecule-functionalized AuNP-based sensors described detail. Finally, we conclude by presenting our subjective views present challenges some appropriate suggestions future research directions sensors.

Language: Английский

Citations

50
Electrochemical Immunoassay for the Detection of SARS-CoV-2 Nucleocapsid Protein in Nasopharyngeal Samples DOI
Isabelle C. Samper, Catherine McMahon,

Melissa S. Schenkel

et al.

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(11), P. 4712 - 4719

Published: March 9, 2022

Point-of-care (POC) methods currently available for detecting SARS-CoV-2 infections still lack accuracy. Here, we report the development of a highly sensitive electrochemical immunoassay capable quantitatively presence virus in patient nasopharyngeal samples using stencil-printed carbon electrodes (SPCEs) functionalized with capture antibodies targeting nucleocapsid protein (N protein). Samples are added to electrode surface, followed by horseradish peroxidase (HRP)-conjugated detection also N protein. The concentration is quantified chronoamperometry 3,3'5,5'-tetramethylbenzidine. Limits equivalent less than 50 plaque forming units/mL (PFU/mL) were determined sample volumes 20 μL. No cross-reactivity was detected influenza and other coronavirus proteins. Patient tested as part proof-of-concept clinical study where gold-standard real-time quantitative polymerase chain reaction (RT-qPCR) method. Preliminary results from data set 22 demonstrated specificity 100% (n = 9 negative according RT-qPCR) sensitivity 70% RT-PCR cycle threshold (Ct) values under 30 10) Ct 25 5), which complies World Health Organization (WHO) criteria POC COVID-19 diagnostic tests. Our SPCEs validated against standard assays, very good agreement found between both (R2 0.9993, n 6), suggesting that our assay could be used assess infectivity. takes 70 min sampling results.

Language: Английский

Citations

43
Highly sensitive and selective surface plasmon resonance biosensor for the detection of SARS-CoV-2 spike S1 protein DOI
Qiong Wu, Wu Wen, Fangfang Chen

et al.

The Analyst, Journal Year: 2022, Volume and Issue: 147(12), P. 2809 - 2818

Published: Jan. 1, 2022

The reality that the coronavirus disease 2019 (COVID-19) is still raging around world and making a comeback with strong presence has highlighted need for rapid sensitive quantitative detection methods of viral RNA, antibody antigen widespread tracking screening applications. Surface plasmon resonance (SPR) technology achieved development become standard measurement method in fields biosensing, biomedicine, biochemistry biopharmaceuticals due to its advantages high sensitivity, fast response no labelling. Here, we report sandwiched structure-based SPR biosensor detecting specific antigen, severe acute respiratory syndrome 2 (SARS-CoV-2) spike S1 protein. sensor combines Ti3C2-MXene nanosheet modified sensing platform polydopamine (PDA)-Ag nanoparticle (AgNP)/anti-SARS-CoV-2 protein nanoconjugate signal enhancers, exhibiting wide linear range 0.0001 1000 ng mL-1 low limit 12 fg (S/N = 3). In analysis artificial saliva human serum samples, proposed exhibits good reproducibility specificity, which indicates potential application complex bodily fluids. exploitation MXene-based biochip recognizing SARS-CoV-2 provides an accessible way COVID-19 diagnosis, promotes 2D nanomaterial-based chips clinical diagnosis screening. Significantly, possesses general applicability can be reprogrammed detect any if corresponding nanobody available.

Language: Английский

Citations

43
Microfluidic point-of-care device for detection of early strains and B.1.1.7 variant of SARS-CoV-2 virus DOI
Jongwon Lim, Robert A. Stavins, Victoria Kindratenko

et al.

Lab on a Chip, Journal Year: 2022, Volume and Issue: 22(7), P. 1297 - 1309

Published: Jan. 1, 2022

Additively manufactured plastic cartridges and low-cost optical reader system to successfully detect the SARS-CoV-2 virus from saliva samples within 30 min.

Language: Английский

Citations

40
Diagnostics and analysis of SARS-CoV-2: current status, recent advances, challenges and perspectives DOI Creative Commons
Tao Dong, Mingyang Wang,

Junchong Liu

et al.

Chemical Science, Journal Year: 2023, Volume and Issue: 14(23), P. 6149 - 6206

Published: Jan. 1, 2023

The disastrous spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has induced public healthcare issues and weakened the global economy significantly. Although SARS-CoV-2 infection is not as fatal initial outbreak, many infected victims suffer from long COVID. Therefore, rapid large-scale testing critical in managing patients alleviating its transmission. Herein, we review recent advances techniques to detect SARS-CoV-2. sensing principles are detailed together with their application domains analytical performances. In addition, advantages limits each method discussed analyzed. Besides molecular diagnostics antigen antibody tests, also neutralizing antibodies emerging variants. Further, characteristics mutational locations different variants epidemiological features summarized. Finally, challenges possible strategies prospected develop new assays meet diagnostic needs. Thus, this comprehensive systematic detection technologies may provide insightful guidance direction for developing tools diagnosis analysis support effective long-term pandemic management control.

Language: Английский

Citations

38
Smart Bandaid Integrated with Fully Textile OECT for Uric Acid Real-Time Monitoring in Wound Exudate DOI Creative Commons
Danilo Arcangeli, Isacco Gualandi, Federica Mariani

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(4), P. 1593 - 1608

Published: March 17, 2023

Hard-to-heal wounds (i.e., severe and/or chronic) are typically associated with particular pathologies or afflictions such as diabetes, immunodeficiencies, compression traumas in bedridden people, skin grafts, third-degree burns. In this situation, it is critical to constantly monitor the healing stages and overall wound conditions allow for better-targeted therapies faster patient recovery. At moment, operation performed by removing bandages visually inspecting wound, putting at risk of infection disturbing stages. Recently, new devices have been developed address these issues monitoring important biomarkers related health status, pH, moisture, etc. contribution, we present a novel textile chemical sensor exploiting an organic electrochemical transistor (OECT) configuration based on poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) uric acid (UA)-selective exudate. The combination special medical-grade materials provides passive sampling system that enables real-time non-invasive analysis fluid: UA was detected benchmark analyte status since represents relevant biomarker infections necrotization processes human tissues. sensors proved reliably reversibly detect concentration synthetic exudate biologically range 220–750 μM, operating flow better mimicking real bed. This forerunner device paves way smart integrated OECT-based wound-healing evaluation.

Language: Английский

Citations

33
Conventional and Novel Diagnostic Tools for the Diagnosis of Emerging SARS-CoV-2 Variants DOI Creative Commons
Vivek P. Chavda,

Disha Valu,

Palak K. Parikh

et al.

Vaccines, Journal Year: 2023, Volume and Issue: 11(2), P. 374 - 374

Published: Feb. 6, 2023

Accurate identification at an early stage of infection is critical for effective care any infectious disease. The “coronavirus disease 2019 (COVID-19)” outbreak, caused by the virus “Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)”, corresponds to current and global pandemic, characterized several developing variants, many which are classified as variants concern (VOCs) “World Health Organization (WHO, Geneva, Switzerland)”. primary diagnosis made using either molecular technique RT-PCR, detects parts viral genome’s RNA, or immunodiagnostic procedures, identify proteins antibodies generated host. As demand RT-PCR test grew fast, inexperienced producers joined market with innovative kits, increasing number laboratories diagnostic field, rendering results increasingly prone mistakes. It difficult determine how outcomes one unnoticed result could influence decisions about patient quarantine social isolation, particularly when patients themselves health providers. development point-of-care testing helps in rapid in-field disease, such can also be used a bedside monitor mapping progression patients. In this review, we have provided readers available techniques their pitfalls detecting emerging VOCs SARS-CoV-2, lastly, discussed AI-ML- nanotechnology-based smart SARS-CoV-2 detection.

Language: Английский

Citations

31
Impedimetric Nanobiosensor for the Detection of SARS-CoV-2 Antigens and Antibodies DOI Creative Commons
Diana Isabel Sandoval Bojórquez, Željko Janićijević, Brenda Palestina Romero

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(2), P. 576 - 586

Published: Feb. 10, 2023

Detection of antigens and antibodies (Abs) is great importance in determining the infection immunity status population, as they are key parameters guiding handling pandemics. Current point-of-care (POC) devices a convenient option for rapid screening; however, their sensitivity requires further improvement. We present an interdigitated gold nanowire-based impedance nanobiosensor to detect COVID-19-associated (receptor-binding domain S1 protein SARS-CoV-2 virus) respective Abs appearing during after infection. The electrochemical spectroscopy technique was used assess changes measured resulting from binding analytes surface chip. After 20 min incubation, sensor demonstrate high about 57 pS·s

Language: Английский

Citations

25
An aM-level sensitive cascade CRISPR-Dx system (ASCas) for rapid detection of RNA without pre-amplification DOI
Yibin Zhang, Yong Chen, Qianling Zhang

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 230, P. 115248 - 115248

Published: March 28, 2023

Language: Английский

Citations

25
Multiplexed detection of viral antigen and RNA using nanopore sensing and encoded molecular probes DOI Creative Commons
Ren Ren, Shenglin Cai, Xiaona Fang

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 14, 2023

Abstract We report on single-molecule nanopore sensing combined with position-encoded DNA molecular probes, chemistry tuned to simultaneously identify various antigen proteins and multiple RNA gene fragments of SARS-CoV-2 high sensitivity selectivity. show that this strategy can directly detect spike (S) nucleocapsid (N) in unprocessed human saliva. Moreover, our approach enables the identification from patient samples using nasal/throat swabs, enabling critical mutations such as D614G, G446S, or Y144del among viral variants. In particular, it discriminate between lineages wild-type B.1.1.7 (Alpha), B.1.617.2 (Delta), B.1.1.539 (Omicron) within a single measurement without need for nucleic acid sequencing. The probes is easily adaptable other targets diseases be expanded depending application required.

Language: Английский

Citations

25