Extracellular vesicle-loaded hydrogels for tissue repair and regeneration

Materials Today Bio, Journal Year: 2022, Volume and Issue: 18, P. 100522 - 100522

Published: Dec. 21, 2022

Extracellular vesicles (EVs) are a collective term for nanoscale or microscale secreted by cells that play important biological roles. Mesenchymal stem class of with the potential self-healing and multidirectional differentiation. In recent years, numerous studies have shown EVs, especially those mesenchymal cells, can promote repair regeneration various tissues and, thus, significant in regenerative medicine. However, due to rapid clearance capacity circulatory system, EVs barely able act persistently at specific sites target tissues. Hydrogels good biocompatibility loose porous structural properties allow them serve as EV carriers, thereby prolonging retention certain areas slowing release EVs. When needed function sites, EV-loaded hydrogels stand an excellent approach. this review, we first introduce sources, roles, extraction characterization methods describe their current application status. We then review different types discuss factors influencing abilities carry summarize several strategies loading into characterizing hydrogels. Furthermore, applications tissue repair. This article concludes summary state research on outlook future directions, which hope will provide promising ideas researchers.

Language: Английский

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Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages

Journal of Nanobiotechnology, Journal Year: 2022, Volume and Issue: 20(1)

Published: Jan. 20, 2022

Osteoarthritis (OA) is a degenerative illness that greatly impacts the life quality of patients. Currently, therapeutic approaches for OA are very limited in clinical. The extracellular vesicles (EVs) derived from different mesenchymal stem cells displayed prominent effect on OA. But most EVs have resources and risks host rejection, immunological response, etc. Human umbilical cord (hUCMSCs) hold advantages easy availability, minimal immune excellent immunomodulatory effects, although hUCMSCs-EVs seldom been applied Herein, we investigated potential anti-inflammatory effects treatment In our results, promoted polarization M2-type macrophages expression anti-inflammation-related cytokines (IL-10). Notably, supernate M2 induced by inhibited level inflammation-associated factors chondrocytes caused IL-1β. Further, injection articular lumen ameliorated progression exerted chondroprotective based joint model created surgical transection anterior cruciate ligament (ACLT). addition, found five highly enriched miRNAs hUCMSCs-EVs, including has-miR-122-5p, has-miR-148a-3p, has-miR-486-5p, has-miR-let-7a-5p, has-miR-100-5p High-throughput sequencing miRNAs, with targeted genes mainly PI3K-Akt signaling pathway. Furthermore, also detected protein abundance using liquidation chromatography tandem quadrupole mass spectrometry (LC-MS/MS) analysis. Thus, study indicates can alleviate cartilage degradation during progression, mechanically may through delivering key proteins modulating pathway mediated to promote macrophage, exhibiting potent potential. current findings suggest might serve as new reagent therapy

Language: Английский

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Current Understanding of Hydrogel for Drug Release and Tissue Engineering

Gels, Journal Year: 2022, Volume and Issue: 8(5), P. 301 - 301

Published: May 15, 2022

Due to their good absorption, satisfactory biocompatibility, and high safety, hydrogels have been widely used in the field of biomedicine, including for drug delivery tissue regeneration. In this review, we introduce characteristics crosslinking methods natural synthetic hydrogels. Then, highlight design principle intelligent (i.e., responsive hydrogels) release. Moreover, application release engineering, limitations research directions hydrogel engineering are also considered. We hope that review can provide a reference follow-up studies related fields.

Language: Английский

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Functional biomaterials for osteoarthritis treatment: From research to application

Smart Medicine, Journal Year: 2022, Volume and Issue: 1(1)

Published: Dec. 1, 2022

Osteoarthritis (OA) is a common disease that endangers millions of middle-aged and elderly people worldwide. Researchers from different fields have made great efforts achieved remarkable progress in the pathogenesis treatment OA. However, there still no cure for In this review, we discuss OA summarize current clinical therapies. Moreover, introduce various natural synthetic biomaterials drug release, cartilage transplantation, joint lubricant during treatment. We also present our perspectives insights on future. hope review will foster communication collaboration among biological, clinical, biomaterial researchers, paving way therapeutic breakthroughs.

Language: Английский

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Tailoring Hyaluronic Acid Hydrogels for Biomedical Applications

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 33(49)

Published: Sept. 7, 2023

Abstract Hyaluronic acid (HA) is an attractive anionic polysaccharide polymer with inherent pharmacological properties and versatile chemical groups for modification. Due to their water retention ability, biocompatibility, biodegradation, cluster of differentiation‐44 targeting, highly designable capacity, HA hydrogels have been emerging biomaterial, showing tailoring performance in terms modifications hydrogel forms. Various preparation technologies developed the fabrication unique structures functions. They utilized diverse biomedical applications like drug delivery tissue engineering scaffolds. Herein, this review comprehensively summarizes derivatives different molecule weights functional modifications. Then various methods obtain forms nanogel, nanofiber, microparticle, microneedle patch, injectable hydrogel, scaffold are reviewed as well. The emphasis focused on shining these anti‐bacteria, anti‐inflammation, wound healing, cancer treatment, regenerative medicine, psoriasis diagnosis, etc. potentials prospects subsequently given inspire further investigation, aiming at accelerating product translation from research clinic.

Language: Английский

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Progress of Microfluidic Hydrogel‐Based Scaffolds and Organ‐on‐Chips for the Cartilage Tissue Engineering

Advanced Materials, Journal Year: 2023, Volume and Issue: 35(26)

Published: Jan. 12, 2023

Abstract Cartilage degeneration is among the fundamental reasons behind disability and pain across globe. Numerous approaches have been employed to treat cartilage diseases. Nevertheless, none shown acceptable outcomes in long run. In this regard, convergence of tissue engineering microfabrication principles can allow developing more advanced microfluidic technologies, thus offering attractive alternatives current treatments traditional constructs used applications. Herein, developments involving hydrogel‐based scaffolds, promising structures for regeneration, ranging from hydrogels with channels prepared by devices, that enable therapeutic delivery cells, drugs, growth factors, as well cartilage‐related organ‐on‐chips are reviewed. Thereafter, anatomy types damages, present treatment options briefly overviewed. Various introduced, advantages scaffolds over thoroughly discussed. Furthermore, available technologies fabricating chips presented. The preclinical clinical applications regeneration development time further explained. developments, recent key challenges, prospects should be considered so develop systems repair highlighted.

Language: Английский

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Emerging 3D bioprinting applications in plastic surgery

Biomaterials Research, Journal Year: 2023, Volume and Issue: 27(1)

Published: Jan. 3, 2023

Plastic surgery is a discipline that uses surgical methods or tissue transplantation to repair, reconstruct and beautify the defects deformities of human tissues organs. Three-dimensional (3D) bioprinting has gained widespread attention because it enables fine customization implants in patient's area preoperatively while avoiding some adverse reactions complications traditional approaches. In this paper, we review recent research advances application 3D plastic surgery. We first introduce printing process basic principles technology, revealing advantages disadvantages different technologies. Then, describe currently available materials, dissect rationale for special dynamic (4D bioprinting) achieved by varying combination strategy materials. Later, focus on viable clinical applications effects Finally, summarize discuss challenges prospects believe can contribute further development provide lessons related research.

Language: Английский

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3D-printed fish gelatin scaffolds for cartilage tissue engineering

Bioactive Materials, Journal Year: 2023, Volume and Issue: 26, P. 77 - 87

Published: Feb. 24, 2023

Knee osteoarthritis is a chronic disease caused by the deterioration of knee joint due to various factors such as aging, trauma, and obesity, nonrenewable nature injured cartilage makes treatment challenging. Here, we present three-dimensional (3D) printed porous multilayer scaffold based on cold-water fish skin gelatin for osteoarticular regeneration. To make scaffold, was combined with sodium alginate increase viscosity, printability, mechanical strength, hybrid hydrogel according pre-designed specific structure using 3D printing technology. Then, scaffolds underwent double-crosslinking process enhance their strength even further. These mimic original network in way that allows chondrocytes adhere, proliferate, communicate each other, transport nutrients, prevent further damage joint. More importantly, found were nonimmunogenic, nontoxic, biodegradable. We also implanted into defective rat 12 weeks achieved satisfactory repair results this animal model. Thus, may have broad application potential regenerative medicine.

Language: Английский

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In Situ Sustained Macrophage-Targeted Nanomicelle–Hydrogel Microspheres for Inhibiting Osteoarthritis

Research, Journal Year: 2023, Volume and Issue: 6

Published: Jan. 1, 2023

There are still challenges in applying drug nanocarriers for situ sustained macrophage targeting and regulation, due to the rapid clearance of burst release vivo. Herein, a nanomicelle-hydrogel microsphere, characterized by its macrophage-targeted nanosized secondary structure that allows it accurately bind M1 macrophages through active endocytosis, is employed addresses insufficient osteoarthritis therapeutic efficacy caused nanocarriers. The 3-dimensional microsphere can prevent escape nanomicelle, thus keeping joints, while ligand-guided carry drugs target enter macrophages, via transition from hydrophobicity hydrophilicity nanomicelles under inflammatory stimulation inside macrophages. experiments show sustainably regulate more than 14 days attenuate local "cytokine storm" continuous apoptosis promotion polarization inhibition. This micro/nano-hydrogel system shows excellent ability macrophage, realizes improvement utilization thereby be potential platform treating macrophage-related diseases.

Language: Английский

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Pearl Powder Hybrid Bioactive Scaffolds from Microfluidic 3D Printing for Bone Regeneration

Advanced Science, Journal Year: 2023, Volume and Issue: 10(34)

Published: Oct. 23, 2023

The development of bioactive scaffolds by mimicking bone tissue extracellular matrix is promising for regeneration. Herein, inspired the composition, a novel pearl powder (PP) hybrid fish gelatin methacrylate (GelMa) hydrogel scaffold loaded with vascular endothelial growth factor (VEGF) regeneration presented. With help microfluidic-assisted 3D printing technology, composition and structure can be accurately controlled to meet clinical requirements. combination skin GelMa PP also endowed good biocompatibility, cell adhesion, osteogenic differentiation ability. Moreover, release VEGF enables promote angiogenesis. Thus, in proposed could accelerated under synergic effect osteogenesis angiogenesis, which has been proved rat skull defect model. These features indicate that will an ideal candidate applications.

Language: Английский

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