Decorated bacteria and the application in drug delivery

Advanced Drug Delivery Reviews, Journal Year: 2022, Volume and Issue: 188, P. 114443 - 114443

Published: July 8, 2022

Language: Английский

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Promising dawn in tumor microenvironment therapy: engineering oral bacteria

International Journal of Oral Science, Journal Year: 2024, Volume and Issue: 16(1)

Published: March 13, 2024

Abstract Despite decades of research, cancer continues to be a major global health concern. The human mouth appears multiplicity local environments communicating with other organs and causing diseases via microbes. Nowadays, the role oral microbes in development progression has received increasing scrutiny. At same time, bioengineering technology nanotechnology is growing rapidly, which physiological activities natural bacteria are modified improve therapeutic efficiency cancers. These engineered were transformed achieve directed genetic reprogramming, selective functional reorganization precise control. In contrast endotoxins produced by typical genetically bacteria, flora exhibits favorable biosafety characteristics. To outline current cognitions upon microbes, cancers, related literatures searched reviewed based on PubMed database. We focused number mechanisms associated tumor microenvironment, involve occurrence development. Whether engineering can possible application therapy worth consideration. A deeper understanding relationship between may enhance our knowledge pathogenesis thus providing new insights strategies for prevention treatment.

Language: Английский

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Innovative Applications of Bacteria and Their Derivatives in Targeted Tumor Therapy

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Despite significant progress in cancer treatment, traditional therapies still face considerable challenges, including poor targeting, severe toxic side effects, and the development of resistance. Recent advances biotechnology have revealed potential bacteria their derivatives as drug delivery systems for tumor therapy by leveraging biological properties. Engineered bacteria, Escherichia coli, Salmonella, Listeria monocytogenes, along with derivatives─outer membrane vesicles (OMVs), bacterial ghosts (BGs), spores (BSPs)─can be loaded a variety antitumor agents, enabling precise targeting sustained release within microenvironment (TME). These possess intrinsic properties that stimulate immune system, enhancing both innate adaptive responses to further amplify therapeutic effects. The ability naturally accumulate hypoxic regions versatility genetic modifications allow tailored strategies synergistically enhance effectiveness chemotherapy, immunotherapy, targeted therapies. This review comprehensively examines fundamental principles therapy, focusing on employed engineering, loading, use therapy. It also discusses challenges faced optimizing systems, such safety concerns, unintended responses, scalability clinical applications. By exploring these aspects, this provides theoretical framework improving bacterial-based contributing more effective personalized treatments.

Language: Английский

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Emerging pyroptosis-engineered nanobiotechnologies regulate cancers and inflammatory diseases: A double-edged sword

Matter, Journal Year: 2022, Volume and Issue: 5(11), P. 3740 - 3774

Published: Nov. 1, 2022

Language: Английский

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Nanomedicine‐Enabled/Augmented Cell Pyroptosis for Efficient Tumor Nanotherapy

Advanced Science, Journal Year: 2022, Volume and Issue: 9(35)

Published: Oct. 20, 2022

The terrible morbidity and mortality of malignant tumors urgently require innovative therapeutics, especially for apoptosis-resistant tumors. Pyroptosis, a pro-inflammatory form programmed cell death (PCD), is featured with pore formation in plasma membrane, swelling giant bubbles, leakage cytoplasmic cytokines, which can remodel the tumor immune microenvironment by stimulating "cold" to be an immunogenic "hot" microenvironment, consequently augment therapeutic efficiency Benefiting from current advances nanotechnology, nanomedicine extensively applied potentiate, enable, pyroptosis enhancing cancer-therapeutic efficacy specificity. This review provides concentrated summary discussion most recent progress achieved this emerging field, highlighting nanomedicine-enabled/augmented specific strategy favoring construction next-generation nanomedicines efficiently induce PCD. It highly expected that further clinical translation accelerated inducing pyroptotic based on bioactive nanomedicines.

Language: Английский

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Sonodynamic-immunomodulatory nanostimulators activate pyroptosis and remodel tumor microenvironment for enhanced tumor immunotherapy

Theranostics, Journal Year: 2023, Volume and Issue: 13(5), P. 1571 - 1583

Published: Jan. 1, 2023

Rationale: Spatiotemporal control of pyroptosis has a profound impact on cancer immunotherapy.Owing to the precise spatiotemporal and reduction in side effects ultrasound (US), sonodynamic therapy (SDT) is expected be promising mean activate pyroptosis.Furthermore, pyroptosis-initiated immune response can amplified by enhanced lymphocyte infiltration occurring due extracellular matrix (ECM) depletion.Therefore, it highly desirable develop sonodynamic-immunomodulatory strategy amplify pyroptosis-mediated tumor immunotherapy remodeling microenvironment, thereby enhancing immunotherapy.Methods: We reported potent based sonosensitizer, which composed LY364947-loaded porous coordination network (PCN-224) camouflaged with red blood cell (RBC) membrane evaluated activation, collagen depletion, immunocyte infiltration, adaptive during vitro vivo.Results: The sonosensitizer generated reactive oxygen species (ROS) under US irradiation initiated caspase-3 apoptotic signaling pathway, regarded as key upstream activator gasdermin E (GSDME)-mediated pyroptosis.During subsequent anti-tumor mediated pyroptosis, LY364947 loosened ECM structure via resulting T-lymphocyte nearly complete eradication tumors mouse model formation immunological memory. Conclusion:Our findings indicate that pyroptotic exhibits robust efficacy well provides novel insights into role immunology.

Language: Английский

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Bacterial‐Mediated Tumor Therapy: Old Treatment in a New Context

Advanced Science, Journal Year: 2023, Volume and Issue: 10(12)

Published: March 12, 2023

Abstract Targeted therapy and immunotherapy have brought hopes for precision cancer treatment. However, complex physiological barriers tumor immunosuppression result in poor efficacy, side effects, resistance to antitumor therapies. Bacteria‐mediated provides new options address these challenges. Thanks their special characteristics, bacteria excellent ability destroy cells from the inside induce innate adaptive immune responses. Furthermore, bacterial components, including vesicles, spores, toxins, metabolites, other active substances, similarly inherit unique targeting properties capabilities. Bacteria accessory products can even be reprogrammed produce deliver agents according clinical needs. This review first discusses role of different development tumorigenesis latest advances bacteria‐based delivery platforms existing obstacles application. Moreover, prospect challenges transformation engineered are also summarized.

Language: Английский

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Bacteria-based immunotherapy for cancer: a systematic review of preclinical studies

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 3, 2023

Immunotherapy has been emerging as a powerful strategy for cancer management. Recently, accumulating evidence demonstrated that bacteria-based immunotherapy including naive bacteria, bacterial components, and derivatives, can modulate immune response via various cellular molecular pathways. The key mechanisms of antitumor immunity include inducing cells to kill tumor directly or reverse the immunosuppressive microenvironment. Currently, antigens synthesized vaccine candidates by bioengineering technology are novel immunotherapy. Especially combination therapy with conventional therapies may further achieve enhanced therapeutic benefits against cancers. However, clinical translation is limited biosafety concerns non-uniform production standards. In this review, we aim summarize strategies based on advanced therapeutics discuss their potential management, will also propose approaches optimizing facilitating translation.

Language: Английский

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Harnessing Engineered Immune Cells and Bacteria as Drug Carriers for Cancer Immunotherapy

ACS Nano, Journal Year: 2023, Volume and Issue: 17(2), P. 843 - 884

Published: Jan. 4, 2023

Immunotherapy continues to be in the spotlight of oncology therapy research past few years and has been proven a promising option modulate one's innate adaptive immune systems for cancer treatment. However, poor delivery efficiency agents, potential off-target toxicity, nonimmunogenic tumors significantly limit its effectiveness extensive application. Recently, emerging biomaterial-based drug carriers, including but not limited cells bacteria, are expected candidates break dilemma immunotherapy, with their excellent natures intrinsic tumor tropism immunomodulatory activity. More than that, tiny vesicles physiological components derived from them have similar functions source due inheritance various surface signal molecules proteins. Herein, we presented representative examples about latest advances employed cells, derivatives. Simultaneously, opportunities challenges bacteria-based carriers discussed provide reference future application immunotherapy.

Language: Английский

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Biomaterials Elicit Pyroptosis Enhancing Cancer Immunotherapy

Advanced Functional Materials, Journal Year: 2023, Volume and Issue: 34(7)

Published: Nov. 5, 2023

Abstract Cancer immunotherapy has the potential to revolutionize treatment of malignant tumors, but its effectiveness is limited by low immune response rate and immune‐related adverse events. Pyroptosis, as an inflammatory programmed cell death type, triggers strong acute antitumor immunity, converting “cold” tumors “hot”. Particularly, biomaterials loading pyroptosis inducers targeting tumor microenvironment engineer pyroptosis, have achieved great progress in recent years. Herein, design strategy, mechanism pathway, role induce cancer are comprehensively reviewed. The present review focuses on application biomaterials‐induced immunotherapy, including nanogel, polymer prodrug, nanovesicle, mesoporous material. Additionally, synthesis a series stimuli‐responsive nanoplatforms, glutathione‐responsive, pH‐responsive, reactive oxygen species‐responsive, enzyme‐mimicking catalytic performance, described. Meanwhile, it augments multiple processes uptake, antigen presentation, T‐cell activation, expansion. Finally, perspectives pyroptosis‐mediated inflammation break through vascular basement membrane barrier achieving efficient volcanic penetration discussed. Artificial intelligence, multi‐omics analysis, anthropogenic animal models organoids presented, aiming provide guidance assistance for constructing effective controllable pyroptosis‐engineered improving immunotherapy.

Language: Английский

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