Empirical Optimization of Peptide Sequence and Nanoparticle Colloidal Stability: The Impact of Surface Ligands and Implications for Colorimetric Sensing

ACS Applied Materials & Interfaces, Journal Year: 2023, Volume and Issue: 15(16), P. 20483 - 20494

Published: April 14, 2023

Surface ligands play a critical role in controlling and defining the properties of colloidal nanocrystals. These aspects have been exploited to design nanoparticle aggregation-based colorimetric sensors. Here, we coated 13-nm gold nanoparticles (AuNPs) with large library (e.g., from labile monodentate monomers multicoordinating macromolecules) evaluated their aggregation propensity presence three peptides containing charged, thiolate, or aromatic amino acids. Our results show that AuNPs polyphenols sulfonated phosphine were good choices for electrostatic-based aggregation. capped citrate labile-binding polymers worked well dithiol-bridging π–π stacking-induced In example assays, stress sensing performance requires aggregating low charge valence paired charged NPs weak stability vice versa. We then present modular peptide versatile residues agglomerate variety ligated detection coronavirus main protease. Enzymatic cleavage liberates segment, which turn triggers NP agglomeration thus rapid color changes <10 min. The protease limit is 2.5 nM.

Language: Английский

---

The Potential of Optical Technologies in Early Virus Detection; Prospects in Addressing Future Viral Outbreaks

Critical Reviews in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: March 27, 2025

The urgent need for sensitive, rapid, and reliable diagnostic methodologies to control prevent life-threatening pandemic infectious disease, such as COVID-19, remains a critical priority. Timely on-site detection of viral pathogens is essential effective disease management mitigation societal disruptions. Recent advancements in optical methods have positioned them at the forefront healthcare diagnostics, offering high sensitivity specificity viable alternatives conventional techniques Polymerase Chain Reaction (PCR), which often suffer from time delays limited accessibility resource-constrained environments. This review elucidates potential various techniques, highlighting their advantages over traditional methods. It encompasses range modalities, including fluorescence-based approaches, Raman spectroscopy (RS), Plasmonic (e.g., surface plasmon resonance (SPR), localized SPR, (LSPR), surface-enhanced (SERS), fluorescence (SEF)), super resolution microscopies (SRMs), attenuated total reflectance-Fourier transform infrared (ATR-FTIR), integrated platforms waveguides molecularly imprinted polymer (MIP)-based biosensors. Additionally, evolution novel biosensors, particularly 5th 6th generation challenges related these technologies were discussed. studies reviewed aims advance development portable, specific, cost-effective point-of-care (POC) devices rapid pathogens.

Language: Английский

---

Metasurface biosensors: Status and prospects

Applied Physics Reviews, Journal Year: 2025, Volume and Issue: 12(2)

Published: April 4, 2025

Metasurfaces have emerged as a rapidly evolving frontier in the fields of optics and photonics, with growing emphasis on their potential for practical applications. The considerable volume contributions to study metasurfaces has expanded, creating challenges tracking all advancements within this dynamic field. In review, we select practically useful among diverse studied so far. We refer selected hot research topics at beginning, succeedingly outline status several applications that are nearing applications, then focus biosensing particular attention metasurface fluorescence (FL) biosensors, because FL detection is major approach bioscience biotechnology. However, by biosensors not been reviewed an extensive comprehensive manner. Indeed, demonstrated capability detecting wide range biomolecules including nucleic acids proteins, such antigens antibodies. Notably, they offer enhanced sensitivity assays reduced assay time when compared conventional commercial assays. here provide focused review current future directions biosensors.

Language: Английский

---

A heparan sulfate proteoglycan-mimicking AIE fluorescent probe for SARS-CoV-2 detection

New Journal of Chemistry, Journal Year: 2024, Volume and Issue: 48(10), P. 4208 - 4212

Published: Jan. 1, 2024

A heparan sulfate proteoglycan (HSPG)-mimicking fluorescent probe, tetrasulfonated tetraphenylethene (TPES), with aggregation-induced emission (AIE) characteristics is developed for fast SARS-CoV-2 detection.

Language: Английский

---

Multicolor-Assay-on-a-Chip Processed by Robotic Operation (MACpro) with Improved Diagnostic Accuracy for Field-Deployable Detection

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(17), P. 6634 - 6642

Published: April 15, 2024

The ability to deploy decentralized laboratories with autonomous and reliable disease diagnosis holds the potential deliver accessible healthcare services for public safety. While microfluidic technologies provide precise manipulation of small fluid volumes improved assay performance, their limited automation versatility confine them laboratories. Herein, we report utility multicolor assay-on-a-chip processed by robotic operation (MACpro), address this unmet need. MACpro platform comprises a robot-microfluidic interface an eye-in-hand module that provides flexible yet stable actions execute tasks in programmable manner, such as chip along different paths. Notably, shows detection performance integrating microbead-based antibody immobilization enhanced target recognition sensing via Cu2+-catalyzed plasmonic etching gold nanorods rapid sensitive analyte quantification. Using interferon-gamma example, demonstrate completes sample-to-answer immunoassay within 30 min achieves 10-fold broader dynamic range lower limit compared standard enzyme-linked immunosorbent assays (0.66 vs 5.2 pg/mL). extends applications beyond traditional presents automated solution expand diagnostic capacity diverse settings.

Language: Английский

---

Air sampling and simultaneous detection of airborne influenza virus via gold nanorod-based plasmonic PCR

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 477, P. 135180 - 135180

Published: July 14, 2024

Language: Английский

---

The current emergence of monkeypox: The recurrence of another smallpox?

Biosafety and Health, Journal Year: 2022, Volume and Issue: 4(6), P. 369 - 375

Published: Sept. 17, 2022

Language: Английский

---

Two-Way Detection of COVID-19 Spike Protein and Antibody Using All-Dielectric Metasurface Fluorescence Sensors

Biosensors, Journal Year: 2022, Volume and Issue: 12(11), P. 981 - 981

Published: Nov. 7, 2022

COVID-19 (or SARS-CoV-2) has deeply affected human beings worldwide for over two years, and its flexible mutations indicate the unlikeliness of termination in a short time. Therefore, it is important to develop quantitative platform direct detection status monitoring. Such should be simpler than nucleic acid amplification techniques such as polymerase chain reaction, more reliable disposable test kits that are based on immunochromatography. To fulfill these requirements, we conducted proof-of-concept experiments spike glycoprotein peptides antibodies one platform, i.e., all-dielectric metasurface fluorescence (FL) sensors. The high capability enhance FL intensity enabled us quantitatively measure glycoproteins efficiently compared with previous methods reported date. Furthermore, intrinsic limit sensors was examined via confocal microscopy found less 0.64 pg/mL peptides. Moreover, had dynamic range five orders target concentrations, indicating extremely sensitivity. These two-way functions can helpful reducing daily loads clinics providing values proper diagnosis cures.

Language: Английский

---

Binding-Induced Folding of DNA Oligonucleotides Targeted to the Nucleocapsid Gene Enables Electrochemical Sensing of SARS-CoV-2

ACS Applied Bio Materials, Journal Year: 2023, Volume and Issue: 6(3), P. 1133 - 1145

Published: March 6, 2023

In the wake of COVID-19 pandemic, millions confirmed cases and deaths have been reported around world. spread can be slowed eventually stopped by a rapid test to diagnose positive disease on spot. It is still important for quickly regardless availability vaccine. Using binding-induced folding principle, we developed an electrochemical detecting SARS-CoV-2 with no RNA extraction or nucleic acid amplification. The showed high sensitivity limit detection 2.5 copies/μL. An electrode mounted capture probe portable potentiostat are used conduct test. To target N-gene SARS-CoV-2, highly specific oligo-capturing was used. Based "folding" sensor detects binding between oligo RNA. When absent, tends form hairpin as secondary structure, retaining redox reporter close surface. This seen large anodic cathodic peak current. present, structure will open hybridize its complementary sequence, causing pull away from electrode. Consequently, anodic/cathodic currents reduced, indicating presence genetic material. Validation performance performed using 122 clinical samples (55 positives 67 negatives) benchmarked gold standard reverse transcription-polymerase chain reaction (RT-PCR) As result our test, accuracy, sensitivity, specificity measured at 98.4%, 98.2%, 98.5%, respectively.

Language: Английский

---

Access to and utilization of COVID-19 antigen rapid diagnostic tests (Ag-RDTs) among people living with HIV (PLWH): A mixed methods study from Cambodia

PLOS Global Public Health, Journal Year: 2024, Volume and Issue: 4(2), P. e0002940 - e0002940

Published: Feb. 13, 2024

Several COVID-19 antigen rapid diagnostic tests have been approved in Cambodia, but no evidence exists about the access to and utilization of these tests. This limits public health interventions increase testing, especially among vulnerable populations such as people living with HIV (PLWH). We conducted a mixed method study PLWH Phnom Penh, between July August 2022 understand their current Ag-RDT levels, well key barriers drivers. undertook cross-sectional survey focus group discussions 280 10 PLWH, respectively, from five treatment centres using probability-proportional-to-size simple random sampling approach. Access was defined having received within six months administered Ag-RDT, either oneself or others, 12 prior study. calculated means, standard deviations proportions for continuous categorical variables, linear regression model effects account clustering. Additionally, we fitted logistic assess factors associated access. For qualitative data, used thematic analyses identify barriers/enablers utilization. About 35% (n = 101) reported had an test past months. 11% 32) participants themselves, 4% 10) others 9% 24) done both, Age education appeared be models. Price advice pharmacists were commonly main selection criteria brand chosen. Ag-RDTs are important tool familiarity use price could hinder better uptake,

Language: Английский

---