Antimicrobial polymer-siRNA polyplexes as a dual-mode platform for the treatment of wound biofilm infections DOI
Taewon Jeon, Jessa Marie Makabenta, Jungmi Park

et al.

Materials Horizons, Journal Year: 2023, Volume and Issue: 10(12), P. 5500 - 5507

Published: Jan. 1, 2023

Treatment of wound biofilm infections faces challenges from both pathogens and uncontrolled host immune response. Treating issues through a single vector would provide enhanced healing. Here, we report the use potent cationic antimicrobial polymer to generate siRNA polyplexes for dual-mode treatment biofilms in vivo. These act as an antibiofilm agent delivery vehicle knockdown biofilm-associated pro-inflammatory MMP9 macrophages. The resulting were effective vitro, eradicating MRSA efficiently delivering macrophages vitro with concomitant MMP9. likewise vivo murine model, significantly reducing bacterial load (∼99% clearance) expression by 80% (qRT-PCR). This combination therapeutic strategy dramatically reduced purulence expedited Taken together, these translatable managing biofilm-infected wounds.

Language: Английский

Polymeric Nanoparticles for Drug Delivery DOI
Maximilian A. Beach,

Umeka Nayanathara,

Yanting Gao

et al.

Chemical Reviews, Journal Year: 2024, Volume and Issue: 124(9), P. 5505 - 5616

Published: April 16, 2024

The recent emergence of nanomedicine has revolutionized the therapeutic landscape and necessitated creation more sophisticated drug delivery systems. Polymeric nanoparticles sit at forefront numerous promising designs, due to their unmatched control over physiochemical properties such as size, shape, architecture, charge, surface functionality. Furthermore, polymeric have ability navigate various biological barriers precisely target specific sites within body, encapsulate a diverse range cargo efficiently release this in response internal external stimuli. However, despite these remarkable advantages, presence wider clinical application is minimal. This review will provide comprehensive understanding vehicles. affecting be outlined first, followed by description nanoparticle designs preparation methods, beginning with polymers on which they are based. meticulously explore current performance against myriad diseases including cancer, viral bacterial infections, before finally evaluating advantages crucial challenges that determine potential decades come.

Language: Английский

Citations

236
Applications and advancements of nanoparticle-based drug delivery in alleviating lung cancer and chronic obstructive pulmonary disease DOI
Gabriele De Rubis, Keshav Raj Paudel, Leander Corrie

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2023, Volume and Issue: 397(5), P. 2793 - 2833

Published: Nov. 22, 2023

Language: Английский

Citations

33
Nanotechnology in inflammation: cutting-edge advances in diagnostics, therapeutics and theranostics DOI Creative Commons
Yuting Liu, Ziqi Lin, Yuting Wang

et al.

Theranostics, Journal Year: 2024, Volume and Issue: 14(6), P. 2490 - 2525

Published: Jan. 1, 2024

Inflammatory dysregulation is intimately associated with the occurrence and progression of many life-threatening diseases.Accurate detection timely therapeutic intervention on inflammatory are crucial for effective therapy inflammation-associated diseases.However, clinical outcomes inflammation-involved disorders still unsatisfactory.Therefore, there an urgent need to develop innovative anti-inflammatory strategies by integrating emerging technological innovations traditional therapeutics.Biomedical nanotechnology one promising fields that can potentially transform diagnosis treatment inflammation.In this review, we outline recent advances in biomedical inflammation, special attention paid nanosensors nanoprobes precise inflammation-related diseases, nanotherapeutics, as well nanotheranostics combined applications.Moreover, prospects challenges translation nanomedicines highlighted.

Language: Английский

Citations

15
Recent developments of polymeric delivery systems in gene therapeutics DOI
Yijia Li,

Ruizhen Tian,

Jiayun Xu

et al.

Polymer Chemistry, Journal Year: 2024, Volume and Issue: 15(19), P. 1908 - 1931

Published: Jan. 1, 2024

A brief overview of main strategies to improve the gene transfection efficiency PCVs recently and their applications in certain diseases.

Language: Английский

Citations

10
Recent progress of iron-based nanomaterials in gene delivery and tumor gene therapy DOI Creative Commons

Ya Gong,

Xiao Hu, Ming Chen

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: June 2, 2024

Abstract Gene therapy aims to modify or manipulate gene expression and change the biological characteristics of living cells achieve purpose treating diseases. The safe, efficient, stable exogenous genes in is crucial for success therapy, which closely related vectors used therapy. Currently, are mainly divided into two categories: viral non-viral vectors. Viral widely due advantages persistent expression, high transfection efficiency, but they also have certain issues such as infectivity, immunological rejection, randomness insertion mutation, carcinogenicity, limited vector capacity. Non-viral non-infectivity, controllable chemical structure, unlimited capacity, efficiency low. With rapid development nanotechnology, unique physicochemical properties nanomaterials attracted increasing attention field drug delivery. Among many nanomaterials, iron-based much their superior properties, Fenton reaction, magnetic resonance imaging, magnetothermal photothermal delivery, magnetically-assisted cell tissue targeting, so on. In this paper, research progress delivery tumor reviewed, future application direction further prospected.

Language: Английский

Citations

10
Polymer-siRNA nanovectors for treating lung inflammation DOI
Ritabrita Goswami, Harini Nagaraj, Yağız Anıl Çiçek

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 378, P. 1092 - 1102

Published: Jan. 5, 2025

Language: Английский

Citations

1
Functionalization of polymers for intracellular protein delivery DOI
Yuhe Zhang, Jiahao Shi, Bin Ma

et al.

Progress in Polymer Science, Journal Year: 2023, Volume and Issue: 146, P. 101751 - 101751

Published: Oct. 1, 2023

Language: Английский

Citations

13
Pulmonary siRNA Delivery with Sophisticated Amphiphilic Poly(Spermine Acrylamides) for the Treatment of Lung Fibrosis DOI Creative Commons
Friederike Adams,

Christoph M. Zimmermann,

Domizia Baldassi

et al.

Small, Journal Year: 2023, Volume and Issue: 20(22)

Published: Dec. 21, 2023

Abstract RNA interference (RNAi) is an efficient strategy to post‐transcriptionally silence gene expression. While all siRNA drugs on the market target liver, lung offers a variety of currently undruggable targets, which can potentially be treated with therapeutics. To achieve this goal, synthesis poly(spermine acrylamides) (P(SpAA) reported herein. Polymers are prepared via polymerization N‐acryloxysuccinimide (NAS) and afterward active ester converted into spermine‐based pendant groups. Copolymerizations decylacrylamide employed increase hydrophobicity polymers. After deprotection, polymers show excellent encapsulation obtain perfectly sized polyplexes at very low polymer/RNA ratios. In vitro 2D 3D cell culture, ex vivo in experiments reveal superior properties amphiphilic spermine‐copolymers respect delivery cells comparison commonly used lipid‐based transfection agents. line results, human explants confirm more silencing protease‐activated receptor 2 (PAR2), G protein‐coupled involved fibrosis. This study reveals importance balance between polyplex formation, cellular uptake, knockdown, toxicity for vitro, vivo, fibrotic tissue vivo.

Language: Английский

Citations

13
Inhaled Macrophage Apoptotic Bodies-Engineered Microparticle Enabling Construction of Pro-Regenerative Microenvironment to Fight Hypoxic Lung Injury in Mice DOI Creative Commons
Chang Liu,

Xingping Quan,

Xidong Tian

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(20), P. 13361 - 13376

Published: May 10, 2024

Oxygen therapy cannot rescue local lung hypoxia in patients with severe respiratory failure. Here, an inhalable platform is reported for overcoming the aberrant hypoxia-induced immune changes and alveolar damage using camouflaged poly(lactic-co-glycolic) acid (PLGA) microparticles macrophage apoptotic body membrane (cMAB). cMABs are preloaded mitochondria-targeting superoxide dismutase/catalase nanocomplexes (NCs) modified pathology-responsive growth factor colony-stimulating (CSF) chains, which form a core-shell called C-cMAB/NC efficient deposition deeper alveoli high affinity to epithelial cells (AECs) after CSF chains cleaved by matrix metalloproteinase 9. Therefore, NCs can be effectively transported into mitochondria inhibit inflammasome-mediated AECs mouse models of hypoxic acute injury. Additionally, at-site release sufficient circulating monocytes macrophages alter their phenotypes, maximizing synergetic effects on creating pro-regenerative microenvironment that enables resolution injury inflammation. This may have applications numerous inflammatory diseases.

Language: Английский

Citations

4
Zwitterionic Polymer Lipid Nanoparticles Enabling Selective Organ Targeting Delivery of Small Interfering RNA for the Treatment of Hepatic and Pulmonary Inflammation DOI
Jiaxin Li, Nan Wang, Wei Zhang

et al.

Small, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 4, 2024

Abstract Lipid nanoparticles (LNPs) are clinically advanced small interfering RNA (siRNA) carriers, which can prevent the breakdown of siRNA during delivery and deliver into cytoplasm to down‐regulate target gene. However, clinical LNPs limited liver, enhancing extrahepatic targeting is vital expand application in vivo. Here, zwitterionic polymer (ZP‐LNPs) assembled with polycarboxybetaine (PCB) modified 1,2‐dimyristoylglycerol (DMG) lipid DMG‐PCB n selectively liver lung, respectively. Three libraries 90 ZP‐LNPs established by adjusting degree polymerization , molar ratio lipids, mass between lipids siRNA. Physicochemical vivo biodistribution results show that B4‐3@siRNA B5‐1@siRNA high encapsulation efficiency (>85%) achieve targeted The mechanism findings indicate pKa protein corona essential determine fate tendency for specific organs. Importantly, these two siTNF‐α effectively reduce levels tumor necrosis factor α (TNF‐α) mice hepatic inflammation pulmonary inflammation, respectively, indicating their promising treatment diseases associated lung.

Language: Английский

Citations

4